Magnetic cell separation:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
2008
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Laboratory techniques in biochemistry and molecular biology
32 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 454 S. Ill., graph. Darst. |
| ISBN: | 9780444527547 0444527540 |
Internformat
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| 245 | 1 | 0 | |a Magnetic cell separation |c ed. by M. Zborowski ; J. J. Chalmers |
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Datensatz im Suchindex
| _version_ | 1804137170690113536 |
|---|---|
| adam_text | Contents
Preface xi
Chapter 1. Magnetic susceptibility 1
Maciej Zborowski
1.1. Mass and volume magnetic susceptibilities 3
1.2. Molar magnetic susceptibility 4
1.3. Magnetic susceptibilities in CGS and SI systems of units 5
1.4. Bulk magnetic susceptibility of a mixture 7
1.5. Volume magnetic susceptibility and Bohr magneton 9
1.6. Multiphase magnetic suspensions 14
1.7. Magnetic phase separation 16
References 26
Chapter 2. Magnetic formulary 29
Maciej Zborowski
2.1. Langevin theory of paramagnetism 29
2.2. Paramagnetic substances: lanthanide solutions 32
2.3i Para mag hSf ic s Ubstancfes: hemoglobin and
« its derivatives * 33
2.4V.. SuperparamagBetispaijticles and ferrofluids 35
2.5; Fe^rprrtagnetistrt* and magnetic properties of iron 38
2.6.1 Dia4ifegnet4sm i T . . . . 45
2.7. Magnetic fiekTvectors 46
2.8. Magnetic field magnitude 47
2.9. Magnetic field sources 48
2.10. Field gradients 49
2.11. Magnetic field lines 51
2.12. Magnetic field in matter 53
References 57
V
Vi CONTENTS
Chapter 3. Maxwell stress and magnetic force 63
Maciej Zborowski
3.1. Introduction 63
3.2. Magnetic force density and the body force 64
3.3. Magnetic force acting on a small, magnetically
susceptible particle (diamagnetic and
paramagnetic materials) 66
3.4. Magnetic force on a small particle acting as a permanent
magnetic dipole (ferromagnetic materials) 68
3.5. Potential energy of an elementary magnetic dipole 68
3.6. Magnetic field induced particle motion 70
3.7. Magnetic pathlines 71
3.8. Cell deposition contour surfaces 76
References 78
Chapter 4. Basic magnetic field configurations 81
Maciej Zborowski
4.1. Introduction 81
4.2. Infinite cylinder 81
4.3. Sphere and a stack of spheres 86
4.4. Interpolar gap 88
4.5. Isodynamic field 95
4.6. Quadrupole field 98
4.7. Other magnet configurations 101
References 102
Chapter 5. Magnetophoresis 105
Maciej Zborowski
5.1. Magnetophoretic mobility 105
References 116
CONTENTS Vii
Chapter 6. Synthesis and characterization of
nano and micron sized iron oxide and iron particles
for biomedical applications 119
Shlomo Margel, Tammy Lublin Tennenbaum, Sigalit Gura,
Merav Tsubery, Udi Akiva, Nava Shpaisman, Anna Galperin,
Benny Perlstein, Polina Lapido, Yonit Boguslavsky,
Jenny Goldshtein and Ofra Ziv
6.1. Introduction 120
6.2. Maghemite nanoparticles of narrow size distribution 123
6.3. Air stable iron nanocrystalline particles 135
6.4. Solid and hollow maghemite/polystyrene and silica/maghemite/
polystyrene micron sized composite particles of narrow
size distribution 146
6.5. Magnetic/nonmagnetic polystyrene/poly(methyl methacrylate)
hemispherical composite micron sized particles 154
Summary 156
References 157
Chapter 7. The biocompatibility and toxicity of
magnetic particles 163
Urs O. Hafeli, Jennifer Aue and Jaime Damani
7.1. Introduction 163
7.2. Definition of toxicity and biocompatibility 165
7.3. Particle characteristics that influence toxicity 168
7.4. Testing for particle toxicity and biocompatibility 178
7.5. Immunogenicity of biological targeting reagents 197
7.6. Legal standards for toxicity testing 203
7.7. Conclusions 212
References 214
Chapter 8. Analytical magnetic techniques in biology 225
Jeffrey J. Chalmers and Lee R. Moore
8.1. Introduction 225
8.2. Measurements of magnetic susceptibility 225
Viii CONTENTS
8.3. Measurements of magnetophoretic mobility: cell tracking
velocimetry (CTV) 226
8.4. Simultaneous measurements of sedimentation rate and
magnetophoretic mobility 233
8.5. Magnetophoretic mobility and antibody binding
capacity (ABC) 235
8.6. Antibody magnetic nanoparticle binding to cells 238
8.7. Effect of antibody labeling concentration (antibody titration)
on magnetophoretic mobility 243
References 246
Chapter 9. Preparative applications of magnetic separation
in biology and medicine 249
Jeffrey J. Chalmers, Xiaodong Tong, Oscar Lara and
Lee R. Moore
9.1. Introduction 249
9.2. Examples of positive selection 251
9.3. Depletion of undesirable cells 251
9.4. Enrichment of rare cells by depletion of normal cells 255
References 260
Chapter 10. Commercial magnetic cell separation
instruments and reagents 265
Maciej Zborowski
10.1. Invitrogen DYNAL Magnetic Particle Concentrator 268
10.2. R D Systems Immunicon MagCellect™ Ferrofluid 271
10.3. Miltenyi Biotec GmbH 273
10.4. StemCell Technologies, Inc 279
10.5. Immunicon Corporation 284
10.6. BD Biosciences 287
References 290
CONTENTS ix
Chapter 11. Worked examples of cell sample preparation
and magnetic separation procedures 293
Maciej Zborowski, Lee R. Moore, Kristie Melnik and
Ying Jing
11.1. Dynal® T Cell Negative Isolation Kit 293
11.2. Dynal CD34 Progenitor Cell Selection System 297
11.3. Miltenyi Biotec MACS® T Cell Isolation Kit II (human) 303
11.4. Miltenyi Biotec Direct CD34 Progenitor Cell Isolation Kit .... 308
11.5. MACS Progenitor Cell Kit performance evaluation 316
11.6. Post separation analyses in other magnetic
separation systems 323
References 330
Chapter 12. New challenges and opportunities 331
Maciej Zborowski, P. Stephen Williams, Lee R. Moore,
Jeffrey J. Chalmers and Peter A. Zimmerman
12.1. Introduction 331
12.2. Magnetophoresis and magnetic capture of malaria infected
erythrocytes 332
12.3. Magnetic flow cell sorting 345
12.4. Magnetic field flow fractionation 365
References 398
Appendix A. Nomenclature, abbreviations, units, and
conversion factors 413
A.I. Nomenclature 413
A.2. Abbreviations 417
A.3. Selected units and conversion factors 420
Appendix B. Vector notation 421
References 426
X CONTENTS
Appendix C. Magnetic body force and the
Maxwell stresses 427
C.I. Magnetic body force and the Maxwell stresses 427
C.2. Maxwell stress on a sphere 428
C.3. Magnetic force on a particle in the limit of small
particle size 436
C.4. Elastic body, discrete surface magnetization 440
References 442
Appendix D. Volume magnetic susceptibilities of
selected substances 443
References 444
Index 445
See Color Plate Section in the back of this book
|
| adam_txt |
Contents
Preface xi
Chapter 1. Magnetic susceptibility 1
Maciej Zborowski
1.1. Mass and volume magnetic susceptibilities 3
1.2. Molar magnetic susceptibility 4
1.3. Magnetic susceptibilities in CGS and SI systems of units 5
1.4. Bulk magnetic susceptibility of a mixture 7
1.5. Volume magnetic susceptibility and Bohr magneton 9
1.6. Multiphase magnetic suspensions 14
1.7. Magnetic phase separation 16
References 26
Chapter 2. Magnetic formulary 29
Maciej Zborowski
2.1. Langevin theory of paramagnetism 29
2.2. Paramagnetic substances: lanthanide solutions 32
2.3i Para mag'hSf'ic s Ubstancfes: hemoglobin and
« its derivatives * 33
2.4V. SuperparamagBetispaijticles and ferrofluids 35
2.5; Fe^rprrtagnetistrt* and magnetic properties of iron 38
2.6.1 Dia4ifegnet4sm''i'T . . . . \ 45
2.7. Magnetic "fiekTvectors 46
2.8. Magnetic field magnitude 47
2.9. Magnetic field sources 48
2.10. Field gradients 49
2.11. Magnetic field lines 51
2.12. Magnetic field in matter 53
References 57
V
Vi CONTENTS
Chapter 3. Maxwell stress and magnetic force 63
Maciej Zborowski
3.1. Introduction 63
3.2. Magnetic force density and the body force 64
3.3. Magnetic force acting on a small, magnetically
susceptible particle (diamagnetic and
paramagnetic materials) 66
3.4. Magnetic force on a small particle acting as a permanent
magnetic dipole (ferromagnetic materials) 68
3.5. Potential energy of an elementary magnetic dipole 68
3.6. Magnetic field induced particle motion 70
3.7. Magnetic pathlines 71
3.8. Cell deposition contour surfaces 76
References 78
Chapter 4. Basic magnetic field configurations 81
Maciej Zborowski
4.1. Introduction 81
4.2. Infinite cylinder 81
4.3. Sphere and a stack of spheres 86
4.4. Interpolar gap 88
4.5. Isodynamic field 95
4.6. Quadrupole field 98
4.7. Other magnet configurations 101
References 102
Chapter 5. Magnetophoresis 105
Maciej Zborowski
5.1. Magnetophoretic mobility 105
References 116
CONTENTS Vii
Chapter 6. Synthesis and characterization of
nano and micron sized iron oxide and iron particles
for biomedical applications 119
Shlomo Margel, Tammy Lublin Tennenbaum, Sigalit Gura,
Merav Tsubery, Udi Akiva, Nava Shpaisman, Anna Galperin,
Benny Perlstein, Polina Lapido, Yonit Boguslavsky,
Jenny Goldshtein and Ofra Ziv
6.1. Introduction 120
6.2. Maghemite nanoparticles of narrow size distribution 123
6.3. Air stable iron nanocrystalline particles 135
6.4. Solid and hollow maghemite/polystyrene and silica/maghemite/
polystyrene micron sized composite particles of narrow
size distribution 146
6.5. Magnetic/nonmagnetic polystyrene/poly(methyl methacrylate)
hemispherical composite micron sized particles 154
Summary 156
References 157
Chapter 7. The biocompatibility and toxicity of
magnetic particles 163
Urs O. Hafeli, Jennifer Aue and Jaime Damani
7.1. Introduction 163
7.2. Definition of toxicity and biocompatibility 165
7.3. Particle characteristics that influence toxicity 168
7.4. Testing for particle toxicity and biocompatibility 178
7.5. Immunogenicity of biological targeting reagents 197
7.6. Legal standards for toxicity testing 203
7.7. Conclusions 212
References 214
Chapter 8. Analytical magnetic techniques in biology 225
Jeffrey J. Chalmers and Lee R. Moore
8.1. Introduction 225
8.2. Measurements of magnetic susceptibility 225
Viii CONTENTS
8.3. Measurements of magnetophoretic mobility: cell tracking
velocimetry (CTV) 226
8.4. Simultaneous measurements of sedimentation rate and
magnetophoretic mobility 233
8.5. Magnetophoretic mobility and antibody binding
capacity (ABC) 235
8.6. Antibody magnetic nanoparticle binding to cells 238
8.7. Effect of antibody labeling concentration (antibody titration)
on magnetophoretic mobility 243
References 246
Chapter 9. Preparative applications of magnetic separation
in biology and medicine 249
Jeffrey J. Chalmers, Xiaodong Tong, Oscar Lara and
Lee R. Moore
9.1. Introduction 249
9.2. Examples of positive selection 251
9.3. Depletion of undesirable cells 251
9.4. Enrichment of rare cells by depletion of normal cells 255
References 260
Chapter 10. Commercial magnetic cell separation
instruments and reagents 265
Maciej Zborowski
10.1. Invitrogen DYNAL Magnetic Particle Concentrator 268
10.2. R D Systems Immunicon MagCellect™ Ferrofluid 271
10.3. Miltenyi Biotec GmbH 273
10.4. StemCell Technologies, Inc 279
10.5. Immunicon Corporation 284
10.6. BD Biosciences 287
References 290
CONTENTS ix
Chapter 11. Worked examples of cell sample preparation
and magnetic separation procedures 293
Maciej Zborowski, Lee R. Moore, Kristie Melnik and
Ying Jing
11.1. Dynal® T Cell Negative Isolation Kit 293
11.2. Dynal CD34 Progenitor Cell Selection System 297
11.3. Miltenyi Biotec MACS® T Cell Isolation Kit II (human) 303
11.4. Miltenyi Biotec Direct CD34 Progenitor Cell Isolation Kit . 308
11.5. MACS Progenitor Cell Kit performance evaluation 316
11.6. Post separation analyses in other magnetic
separation systems 323
References 330
Chapter 12. New challenges and opportunities 331
Maciej Zborowski, P. Stephen Williams, Lee R. Moore,
Jeffrey J. Chalmers and Peter A. Zimmerman
12.1. Introduction 331
12.2. Magnetophoresis and magnetic capture of malaria infected
erythrocytes 332
12.3. Magnetic flow cell sorting 345
12.4. Magnetic field flow fractionation 365
References 398
Appendix A. Nomenclature, abbreviations, units, and
conversion factors 413
A.I. Nomenclature 413
A.2. Abbreviations 417
A.3. Selected units and conversion factors 420
Appendix B. Vector notation 421
References 426
X CONTENTS
Appendix C. Magnetic body force and the
Maxwell stresses 427
C.I. Magnetic body force and the Maxwell stresses 427
C.2. Maxwell stress on a sphere 428
C.3. Magnetic force on a particle in the limit of small
particle size 436
C.4. Elastic body, discrete surface magnetization 440
References 442
Appendix D. Volume magnetic susceptibilities of
selected substances 443
References 444
Index 445
See Color Plate Section in the back of this book |
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| index_date | 2024-07-02T18:56:41Z |
| indexdate | 2024-07-09T21:08:02Z |
| institution | BVB |
| isbn | 9780444527547 0444527540 |
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| physical | XVIII, 454 S. Ill., graph. Darst. |
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| publisher | Elsevier |
| record_format | marc |
| series | Laboratory techniques in biochemistry and molecular biology |
| series2 | Laboratory techniques in biochemistry and molecular biology |
| spelling | Magnetic cell separation ed. by M. Zborowski ; J. J. Chalmers 1. ed. Amsterdam [u.a.] Elsevier 2008 XVIII, 454 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Laboratory techniques in biochemistry and molecular biology 32 Cell separation Magnetic separation Cytologie (DE-588)4070177-3 gnd rswk-swf Magnetismus (DE-588)4037021-5 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Zellfraktionierung (DE-588)4304966-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellfraktionierung (DE-588)4304966-7 s Magnetismus (DE-588)4037021-5 s DE-604 Cytologie (DE-588)4070177-3 s Labortechnik (DE-588)4123602-6 s 2\p DE-604 Zborowski, Maciej Sonstige oth Laboratory techniques in biochemistry and molecular biology 32 (DE-604)BV005871299 32 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016141066&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Magnetic cell separation Laboratory techniques in biochemistry and molecular biology Cell separation Magnetic separation Cytologie (DE-588)4070177-3 gnd Magnetismus (DE-588)4037021-5 gnd Labortechnik (DE-588)4123602-6 gnd Zellfraktionierung (DE-588)4304966-7 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4037021-5 (DE-588)4123602-6 (DE-588)4304966-7 (DE-588)4143413-4 |
| title | Magnetic cell separation |
| title_auth | Magnetic cell separation |
| title_exact_search | Magnetic cell separation |
| title_exact_search_txtP | Magnetic cell separation |
| title_full | Magnetic cell separation ed. by M. Zborowski ; J. J. Chalmers |
| title_fullStr | Magnetic cell separation ed. by M. Zborowski ; J. J. Chalmers |
| title_full_unstemmed | Magnetic cell separation ed. by M. Zborowski ; J. J. Chalmers |
| title_short | Magnetic cell separation |
| title_sort | magnetic cell separation |
| topic | Cell separation Magnetic separation Cytologie (DE-588)4070177-3 gnd Magnetismus (DE-588)4037021-5 gnd Labortechnik (DE-588)4123602-6 gnd Zellfraktionierung (DE-588)4304966-7 gnd |
| topic_facet | Cell separation Magnetic separation Cytologie Magnetismus Labortechnik Zellfraktionierung Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016141066&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV005871299 |
| work_keys_str_mv | AT zborowskimaciej magneticcellseparation |