Human cell culture protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2005
|
| Edition: | 2. ed. |
| Series: | Methods in molecular medicine
107 |
| Subjects: | |
| Online Access: | Table of contents Inhaltsverzeichnis Klappentext |
| Physical Description: | XI, 359 S. zahlr. Ill., graph. Darst. |
| ISBN: | 1588292223 9781588292223 |
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| 250 | |a 2. ed. | ||
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| 300 | |a XI, 359 S. |b zahlr. Ill., graph. Darst. | ||
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Record in the Search Index
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| adam_text | In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading
authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range
of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum
of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to
control of proliferation and differentiation, New to this edition are chapters on
gastric arid
Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction out¬
lining the principle behind the technique, lists of the necessary equipment and reagents, and tips on trouble¬
shooting and avoiding known pitfalls.
Wide-ranging and highly practical. Human Cell Culture Protocols, Second Edition, provides novice and
experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
Cutting-edge techniques for culturing
human cells
Applications suitable for the major tissue
groups
New chapters on
and gastric and
Step-by-step instructions that ensure
successful results the first
Contents
Establishment and Maintenance of Normal Human Keratinocyte Cul¬
tures. Cultivation of Normal Human Epidermal Melanocytes in the
Absence of Phorbo! Esters. Isolation and Culture of Human Osteo-
blasts. Human Osteodast Culture from Peripheral Blood Monocytes:
Phenotypic Characterization andQuantitation of
Cnondrocyte Cultures as Models of Cartilage-SpecificGene Regulation.
Human Myoblastsand Muscle-DerivedSP Cells. Cell Cultures of Autop¬
sy-Derived Fibroblasjs. Primaiy Culture and Differentiation of Human
Adipocyie
Culture. Purification of Peripheral Blood Natural Killer Cells. Human
Fetal Brain Cell Culture. Culturing Human Schwann Cells. Well-Differ¬
entiated Human Aiiway Epithelial Cell Cultures. Isolation and Culture
of HumanAlveolar Epithelial CefeANew Approach to
of Human GastncEpMielium. Isolation and Culture
Epithelial Cells Using a Modified ExpIantTechnique Employing a Non-
injurious Approach. Isolation and Culture of Human Hepatocytes.
Glomerular Epithelial and Mesangial Cell Culture and Characterization.
Isolation and Culture of HumanRenal Cortical Cells with Characteristics
of Proximal Tubules. Culture of Parathyroid Cells,
and Maintenance of Human Islets of
Free Media. Primary Culture of Human
Cells. Conjunctiva Organ and Cell Culture. Establishment Mainte¬
nance, andlransfectionofln Vitro Cultures of Human Retinal Pigment
Epithelium. Index.
Contents
Preface
Contributors
1
Keratinocyte Cultures
Claire
2
in the Absence of Phorbol Esters
Mei-Yu Hsu, Ling Li, and Meenhard Herlyn
3
Alison Gartland,
Judith M.
4
Phenotypic Characterization and
Katherine
and James A. Gallager
5
Gene Regulation
Mary B.
6
Grace K. Pavlath and
7
Volker
8
Precursor Cells
Vanessa van
9
YonaKeisari
10
Bice Perussia and Matthew J.
11
Mark P.
VII
viii Contents
12 Culturing Human Schwann
Victor J. Turnbull...............................................................................173
13
M.
James R.
14
Carsten
15
Pierre Chailler and Daniel
16
Using a Modified
a Noninjurious Approach
Hamid
17
Martin Bayliss and Graham Somers
18
and Characterization
Heather M. Wilson and Keith
19
with Characteristics of Proximal Tubules
Gábriellé
20
PerHellman
21
of
Daniel W.
22
Susan B. Curtis and Alison M. J.
23
Monica Berry and Marcus Radburn-Smith
24
of Human Retinal Pigment Epithelium
Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman,
Monte A. DelMonte, and Douglas A. Greene
Index
|
| adam_txt |
In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading
authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range
of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum
of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to
control of proliferation and differentiation, New to this edition are chapters on
gastric arid
Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction out¬
lining the principle behind the technique, lists of the necessary equipment and reagents, and tips on trouble¬
shooting and avoiding known pitfalls.
Wide-ranging and highly practical. Human Cell Culture Protocols, Second Edition, provides novice and
experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
Cutting-edge techniques for culturing
human cells
Applications suitable for the major tissue
groups
New chapters on
and gastric and
Step-by-step instructions that ensure
successful results the first
Contents
Establishment and Maintenance of Normal Human Keratinocyte Cul¬
tures. Cultivation of Normal Human Epidermal Melanocytes in the
Absence of Phorbo! Esters. Isolation and Culture of Human Osteo-
blasts. Human Osteodast Culture from Peripheral Blood Monocytes:
Phenotypic Characterization andQuantitation of
Cnondrocyte Cultures as Models of Cartilage-SpecificGene Regulation.
Human Myoblastsand Muscle-DerivedSP Cells. Cell Cultures of Autop¬
sy-Derived Fibroblasjs. Primaiy Culture and Differentiation of Human
Adipocyie
Culture. Purification of Peripheral Blood Natural Killer Cells. Human
Fetal Brain Cell Culture. Culturing Human Schwann Cells. Well-Differ¬
entiated Human Aiiway Epithelial Cell Cultures. Isolation and Culture
of HumanAlveolar Epithelial CefeANew Approach to
of Human GastncEpMielium. Isolation and Culture
Epithelial Cells Using a Modified ExpIantTechnique Employing a Non-
injurious Approach. Isolation and Culture of Human Hepatocytes.
Glomerular Epithelial and Mesangial Cell Culture and Characterization.
Isolation and Culture of HumanRenal Cortical Cells with Characteristics
of Proximal Tubules. Culture of Parathyroid Cells,
and Maintenance of Human Islets of
Free Media. Primary Culture of Human
Cells. Conjunctiva Organ and Cell Culture. Establishment Mainte¬
nance, andlransfectionofln Vitro Cultures of Human Retinal Pigment
Epithelium. Index.
Contents
Preface
Contributors
1
Keratinocyte Cultures
Claire
2
in the Absence of Phorbol Esters
Mei-Yu Hsu, Ling Li, and Meenhard Herlyn
3
Alison Gartland,
Judith M.
4
Phenotypic Characterization and
Katherine
and James A. Gallager
5
Gene Regulation
Mary B.
6
Grace K. Pavlath and
7
Volker
8
Precursor Cells
Vanessa van
9
YonaKeisari
10
Bice Perussia and Matthew J.
11
Mark P.
VII
viii Contents
12 Culturing Human Schwann
Victor J. Turnbull.173
13
M.
James R.
14
Carsten
15
Pierre Chailler and Daniel
16
Using a Modified
a Noninjurious Approach
Hamid
17
Martin Bayliss and Graham Somers
18
and Characterization
Heather M. Wilson and Keith
19
with Characteristics of Proximal Tubules
Gábriellé
20
PerHellman
21
of
Daniel W.
22
Susan B. Curtis and Alison M. J.
23
Monica Berry and Marcus Radburn-Smith
24
of Human Retinal Pigment Epithelium
Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman,
Monte A. DelMonte, and Douglas A. Greene
Index |
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| edition | 2. ed. |
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| language | English |
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| spelling | Human cell culture protocols ed. by Joanna Picot 2. ed. Totowa, NJ Humana Press 2005 XI, 359 S. zahlr. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 107 Celkweek gtt Cellules humaines - Culture - Manuels de laboratoire Protocollen (zorgsector) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Human cell culture Laboratory manuals Cells, Cultured Laboratory Manuals Cytological Techniques Laboratory Manuals Mensch (DE-588)4038639-9 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Mensch (DE-588)4038639-9 s Zellkultur (DE-588)4067547-6 s b DE-604 Picot, Joanna Sonstige oth Methods in molecular medicine 107 (DE-604)BV011334960 107 http://www.loc.gov/catdir/toc/ecip0416/2004007186.html Table of contents Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015733229&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015733229&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Human cell culture protocols Methods in molecular medicine Celkweek gtt Cellules humaines - Culture - Manuels de laboratoire Protocollen (zorgsector) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Human cell culture Laboratory manuals Cells, Cultured Laboratory Manuals Cytological Techniques Laboratory Manuals Mensch (DE-588)4038639-9 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4038639-9 (DE-588)4067547-6 |
| title | Human cell culture protocols |
| title_auth | Human cell culture protocols |
| title_exact_search | Human cell culture protocols |
| title_exact_search_txtP | Human cell culture protocols |
| title_full | Human cell culture protocols ed. by Joanna Picot |
| title_fullStr | Human cell culture protocols ed. by Joanna Picot |
| title_full_unstemmed | Human cell culture protocols ed. by Joanna Picot |
| title_short | Human cell culture protocols |
| title_sort | human cell culture protocols |
| topic | Celkweek gtt Cellules humaines - Culture - Manuels de laboratoire Protocollen (zorgsector) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Human cell culture Laboratory manuals Cells, Cultured Laboratory Manuals Cytological Techniques Laboratory Manuals Mensch (DE-588)4038639-9 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Celkweek Cellules humaines - Culture - Manuels de laboratoire Protocollen (zorgsector) Wetenschappelijke technieken Wissenschaftliches Arbeiten Human cell culture Laboratory manuals Cells, Cultured Laboratory Manuals Cytological Techniques Laboratory Manuals Mensch Zellkultur |
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