Working with DNA:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York, N.Y. [u.a.]
Taylor & Francis
2007
|
| Edition: | 1. publ. |
| Series: | The basics
|
| Subjects: | |
| Online Access: | Table of contents only Inhaltsverzeichnis |
| Physical Description: | VI, 414 S. graph. Darst. |
| ISBN: | 0415374642 9780415374644 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Abbreviations vii
Preface ix
Chapter 1 Understanding recombinant DNA techniques 1
1.1 The in vitro workbench 1
1.2 When the goal is a clone 4
Chapter 2 The recombinant DNA laboratory 11
2.1 Supplies 11
2.2 Reagents 17
2.3 Technique 24
2.4 Looking at DNA on a gel 35
2.5 Safety 49
2.6 Documentation 58
2.7 Special lab problems 61
Solutions to problems 66
Chapter 3 Solutions, buffers, stocks and cocktails 67
3.1 Solutions, suspensions and slurries 67
3.2 Solubility of macromolecules 69
3.3 Solute concentration 72
3.4 Solution pH 75
3.5 Precipitation methods 76
3.6 Preparing a solution 81
3.7 Buffer considerations 88
3.8 Stock solutions 100
Solutions to problems 105
Chapter 4 Cloning vectors 109
4.1 Plasmids 109
4.2 Viruses 147
4.3 Chimeras and artificial chromosomes 161
4.4 Common applications 167
Solutions to problems 181
Chapter 5 Restriction endonucleases 185
5.1 Recognition sequences 185
5.2 Cleavage sites 189
5.3 Reaction conditions 198
5.4 Restriction mapping 206
Solutions to problems 216
vi Contents
Chapter 6 Polymerases 221
6.1 Overview 221
6.2 DNA labeling 230
6.3 Site specific mutagenesis 234
6.4 DNA sequencing 242
6.5 Reverse transcriptases 257
6.6 Terminal deoxynucleotidyl transferases 268
6.7 RNA polymerases 270
6.8 The special problem of specific activity 275
Solutions to problems 283
Chapter 7 The polymerase chain reaction 291
7.1 Overview 291
7.2 Primer design 292
7.3 Cycle efficiency 306
7.4 Setting up a reaction 316
7.5 Mutagenesis and engineering 325
7.6 Diagnostic methods 353
7.7 Real time or quantitative PCR 356
7.8 Troubleshooting 360
Solutions to problems 367
Chapter 8 Working with DNA: the reasons 375
8.1 Engineering DNA sequences 375
8.2 Identifying DNA sequences 381
8.3 Functioning of DNA sequences 393
Index 401
|
| adam_txt |
Contents
Abbreviations vii
Preface ix
Chapter 1 Understanding recombinant DNA techniques 1
1.1 The in vitro workbench 1
1.2 When the goal is a clone 4
Chapter 2 The recombinant DNA laboratory 11
2.1 Supplies 11
2.2 Reagents 17
2.3 Technique 24
2.4 Looking at DNA on a gel 35
2.5 Safety 49
2.6 Documentation 58
2.7 Special lab problems 61
Solutions to problems 66
Chapter 3 Solutions, buffers, stocks and cocktails 67
3.1 Solutions, suspensions and slurries 67
3.2 Solubility of macromolecules 69
3.3 Solute concentration 72
3.4 Solution pH 75
3.5 Precipitation methods 76
3.6 Preparing a solution 81
3.7 Buffer considerations 88
3.8 Stock solutions 100
Solutions to problems 105
Chapter 4 Cloning vectors 109
4.1 Plasmids 109
4.2 Viruses 147
4.3 Chimeras and artificial chromosomes 161
4.4 Common applications 167
Solutions to problems 181
Chapter 5 Restriction endonucleases 185
5.1 Recognition sequences 185
5.2 Cleavage sites 189
5.3 Reaction conditions 198
5.4 Restriction mapping 206
Solutions to problems 216
vi Contents
Chapter 6 Polymerases 221
6.1 Overview 221
6.2 DNA labeling 230
6.3 Site specific mutagenesis 234
6.4 DNA sequencing 242
6.5 Reverse transcriptases 257
6.6 Terminal deoxynucleotidyl transferases 268
6.7 RNA polymerases 270
6.8 The special problem of specific activity 275
Solutions to problems 283
Chapter 7 The polymerase chain reaction 291
7.1 Overview 291
7.2 Primer design 292
7.3 Cycle efficiency 306
7.4 Setting up a reaction 316
7.5 Mutagenesis and engineering 325
7.6 Diagnostic methods 353
7.7 Real time or quantitative PCR 356
7.8 Troubleshooting 360
Solutions to problems 367
Chapter 8 Working with DNA: the reasons 375
8.1 Engineering DNA sequences 375
8.2 Identifying DNA sequences 381
8.3 Functioning of DNA sequences 393
Index 401 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T17:59:10Z |
| indexdate | 2024-07-09T20:59:11Z |
| institution | BVB |
| isbn | 0415374642 9780415374644 |
| language | English |
| lccn | 2006034192 |
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| physical | VI, 414 S. graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Taylor & Francis |
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| series2 | The basics |
| spelling | Metzenberg, Stan Verfasser aut Working with DNA Stan Metzenberg 1. publ. New York, N.Y. [u.a.] Taylor & Francis 2007 VI, 414 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier The basics ADN recombinant - Recherche - Méthodologie Recombinant DNA Research Methodology DNA, Recombinant Genetic Techniques Labortechnik (DE-588)4123602-6 gnd rswk-swf Rekombinante DNS (DE-588)4225578-8 gnd rswk-swf Rekombinante DNS (DE-588)4225578-8 s Labortechnik (DE-588)4123602-6 s b DE-604 http://www.loc.gov/catdir/toc/ecip073/2006034192.html Table of contents only HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015717326&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Metzenberg, Stan Working with DNA ADN recombinant - Recherche - Méthodologie Recombinant DNA Research Methodology DNA, Recombinant Genetic Techniques Labortechnik (DE-588)4123602-6 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4225578-8 |
| title | Working with DNA |
| title_auth | Working with DNA |
| title_exact_search | Working with DNA |
| title_exact_search_txtP | Working with DNA |
| title_full | Working with DNA Stan Metzenberg |
| title_fullStr | Working with DNA Stan Metzenberg |
| title_full_unstemmed | Working with DNA Stan Metzenberg |
| title_short | Working with DNA |
| title_sort | working with dna |
| topic | ADN recombinant - Recherche - Méthodologie Recombinant DNA Research Methodology DNA, Recombinant Genetic Techniques Labortechnik (DE-588)4123602-6 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| topic_facet | ADN recombinant - Recherche - Méthodologie Recombinant DNA Research Methodology DNA, Recombinant Genetic Techniques Labortechnik Rekombinante DNS |
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