Digital microscopy:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Ausgabe: | 3. ed. |
| Schriftenreihe: | Methods in cell biology
81 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 608 S., [9] Bl. Ill., graph. Darst. |
| ISBN: | 9780123740250 0123740258 |
Internformat
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| 245 | 1 | 0 | |a Digital microscopy |c ed. by Greenfield Sluder ... |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XVII, 608 S., [9] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 81 | |
| 650 | 7 | |a Digitale technieken |2 gtt | |
| 650 | 7 | |a Microscopie |2 gtt | |
| 650 | 4 | |a Traitement d'images - Techniques numériques | |
| 650 | 4 | |a Vidéomicroscopie | |
| 650 | 4 | |a Image Processing, Computer-Assisted |x instrumentation | |
| 650 | 4 | |a Image Processing, Computer-Assisted |x methods | |
| 650 | 4 | |a Image processing |x Digital techniques | |
| 650 | 4 | |a Microscopy |x instrumentation | |
| 650 | 4 | |a Microscopy |x methods | |
| 650 | 4 | |a Video microscopy | |
| 650 | 0 | 7 | |a Digitalkamera |0 (DE-588)4519830-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bildanalyse |0 (DE-588)4145391-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Videomikroskopie |0 (DE-588)4367875-0 |2 gnd |9 rswk-swf |
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| 830 | 0 | |a Methods in cell biology |v 81 |w (DE-604)BV002534878 |9 81 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015715151&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804136609865531392 |
|---|---|
| adam_text | CONTENTS
Contributors
Preface
1.
Greenfield Sluder and Joshua J.
I. Introduction
II. How Microscopes Work
III. Objective Basics
IV. Mounting Video Cameras on the Microscope
Reference
2.
David
I. Introduction
II. Physical Optics: The Superposition of Waves
III. Huygens Principle
IV. Young s Experiment: Two-Slit Interference
V. Diffraction from a Single Slit
VI. The Airy Disk and the Issue of Microscope Resolution
VII.
VIII.
IX. Resolution and Contrast
X. Conclusions
XL Appendix I
XII.
XIII.
References
3.
H. Ernst Keller
I. Key Components of Every Light Microscope
II. Koehler Illumination
vj
4.
Jan Hinsch
I. Introduction
II. Optical Considerations
5.
David E. Wolf
I. Introduction
II. Light Absorption and Beer s Law
III. Atomic Fluorescence
IV. Organic Molecular Fluorescence
V. Excited State Lifetime and Fluorescence Quantum Efficiency
VI. Excited State Saturation
VII.
VIII.
IX. Fluorescence Depolarization
X. Measuring Fluorescence in the Steady State
XI. Construction of a Monochromator
XII.
XIII.
XIV.
XV. The Fluorescence Microscope
XVI.
References
6.
Aaron F. Straight
I. Introduction
II. The Identification of Green Fluorescent Protein
III. Formation of the GFP Chromophore
IV. The Structure of GFP
V. Mutagenesis to Alter the Properties of GFP
VI. Imaging FPs
VII.
VIII.
References 111
Contents
7.
Jennifer C. Waters
I. Introduction
II. Preparing a Specimen for Fluorescence Live-Cell Imaging
III. Choice of Microscope
IV. Wide-Field Illumination of the Specimen
V. Choosing the Best Objective Lens for Your Application
VI. Acquiring Digital Images Over Time
VII.
VIII.
IX. Conclusion
References
8.
Edward H. Hinchdiffe and Greenfield Sluder
I. Introduction
II. The Black and White Video Signal
III. Adjusting the Camera and Video Monitor
IV. Practical Aspects of Coordinately Adjusting Camera and Monitor
References
9.
Joshua
I. Introduction
II. Measuring Gray-Level Information
III. Camera Settings
IV. Contrast Stretching
V. Camera Versus Image Display Controls
References
10.
Kenneth R. Spring
I. Overview
II. Basic Principles
III. Application of
IV. Future Developments in Imaging Detectors
References
11.
E. D. Salmon, Sidney L. Shaw, Jennifer
Paul S. Maddox, Elaine Yeh, and Kerry Bloom
I. Introduction
II. Design Criteria
III. Microscope Design
IV. Cooled
V. Digital Imaging System
VI. Example Applications
References
12.
Ivan Rasnik, Todd French, Ken
I. Introduction
II. Parameters Characterizing Imaging Devices
III. Specific Imaging Detectors and Features
IV. Conclusions
References
13.
Butch Moomaw
I. Overview
II. Sensitivity
III. Dynamic Range
IV. Spatial Resolution Limits
V. Temporal Resolution
VI. Geometric Distortion
VII.
VIII.
IX. Advanced Technology
References
14.
Noise Reduction, Contrast Enhancement, and Feature Extraction
Richard A. Cardullo and Edward H. Hinchcliffe
I. Introduction
II. Digitization of Images
III. Using Gray Values to Quantify Intensity in the Microscope
IV. Noise Reduction
Contents
V.
VI. Transforms, Convolutions, and Further Uses for Digital Masks
VII.
References
15.
Thomas M. Durcan and Edward H. Hinchcliffe
I. Introduction
II. What Is an Image File?
III. Sampling and Resolution
IV. Bit Depth
V.
VI. Color
VII.
VIII.
IX. Video Files
X. Video Codecs
XL Choosing a Codec
XII.
References
16.
Contrast Light Microscopy
E. D. Salmon and Phong
I. Introduction
II. Basics of DIC Image Formation and Microscope Alignment
III. Basics of Video-Enhanced Contrast
IV. Selection of Microscope and Video Components
V. Test Specimens for Microscope and Video Performance
References
17.
David E. Wolf, Champika Samarasekera, and Jason R. Swedlow
I. So You Want to Do Digital Imaging
II. An Illustrative Example
III. What Is an Image?
IV. What Kind of Quantitative Information Do You Want?
V. Quantitative Microscopy: A Tool
VI. Exercise
Contents
VII.
VIII.
IX. Standard Deviations, Standard Errors of the Mean,
and Other Confusions
X. Dynamic Range, Do Not Waste It
XI. Signal-to-Noise Ratio, S/N, and Signal-to-Background Ratio, S/B
XII.
XIII.
XIV.
XV. Flatfield Correction
XVI.
XVII.
XVIII.
XIX.
XX. Exercise
XXI.
XXII.
XXIII.
XXIV.
XXV.
References
18.
Methods, Analysis, and Corrective Actions
Paul C. Goodwin
I. Introduction
II. Rationale
III. Methods
IV. Discussion
V. Summary
References
19.
Ca + and
Nathan O Connor and
I. Introduction
II. Why Ratio Imaging?
III. Properties of the Indicators BCECF and Fura-2
IV. Calibration of the Fluorescence Signal
V. Components of an Imaging Workstation
VI. Experimental Chamber and
VII.
References
Contents xi
20.
Deconvolution, and Pattern Recognition
Yu-li Wang
I. Introduction
II. Adaptive Noise Filtration
III. Deconvolution
IV. Pattern Recognition-Based Image Restoration
V. Prospectus
References
21.
Jason R. Swedlow
I. Introduction
II. Quantitative Imaging of Biological Samples Using
Fluorescence Microscopy
III. Image Blurring in Biological Samples
IV. Applications for Image Deconvolution
V. Concluding Remarks
References
22.
John M. Murray
I. Introduction
II. Setting Up for Quantitative Imaging
III. Correcting
IV. Limitations to Exact Quantitation
References
23.
Resonance Energy Transfer Microscopy
Richard A. Cardullo
I. Introduction
II. Principles and Basic Methods of FRET
III. FRET Microscopy
IV.
References
xii Contents
24.
Ching- Wei Chang, Dhruv
I. Introduction
II. Fluorophore Excited State Lifetime:
III. Methods for Creating Intracellular Lifetime Maps
IV. FLIM Techniques for Quantitative Cell Biology
V. Summary
References
25.
in Solution and in Living Cells
Dylan A. Bulseco and David E. Wolf
I. Introduction
II. Studying Biological Systems with FCS
III. Designing and Building an FCS Instrument
IV. What Are the Current Commercial Sources of FCS?
V. Summary
References
26.
Rainer
I. Introduction
II. What Is Resolution?
III. Methods Within the Combined Illumination
and Detection Abbe Limit
IV. Methods Circumventing the Abbe Limit
V. A Comment on Near Field Methods and Pendry s Lens
VI. Key Points
References
Index
Volumes in Series
|
| adam_txt |
CONTENTS
Contributors
Preface
1.
Greenfield Sluder and Joshua J.
I. Introduction
II. How Microscopes Work
III. Objective Basics
IV. Mounting Video Cameras on the Microscope
Reference
2.
David
I. Introduction
II. Physical Optics: The Superposition of Waves
III. Huygens' Principle
IV. Young's Experiment: Two-Slit Interference
V. Diffraction from a Single Slit
VI. The Airy Disk and the Issue of Microscope Resolution
VII.
VIII.
IX. Resolution and Contrast
X. Conclusions
XL Appendix I
XII.
XIII.
References
3.
H. Ernst Keller
I. Key Components of Every Light Microscope
II. Koehler Illumination
vj
4.
Jan Hinsch
I. Introduction
II. Optical Considerations
5.
David E. Wolf
I. Introduction
II. Light Absorption and Beer's Law
III. Atomic Fluorescence
IV. Organic Molecular Fluorescence
V. Excited State Lifetime and Fluorescence Quantum Efficiency
VI. Excited State Saturation
VII.
VIII.
IX. Fluorescence Depolarization
X. Measuring Fluorescence in the Steady State
XI. Construction of a Monochromator
XII.
XIII.
XIV.
XV. The Fluorescence Microscope
XVI.
References
6.
Aaron F. Straight
I. Introduction
II. The Identification of Green Fluorescent Protein
III. Formation of the GFP Chromophore
IV. The Structure of GFP
V. Mutagenesis to Alter the Properties of GFP
VI. Imaging FPs
VII.
VIII.
References 111
Contents
7.
Jennifer C. Waters
I. Introduction
II. Preparing a Specimen for Fluorescence Live-Cell Imaging
III. Choice of Microscope
IV. Wide-Field Illumination of the Specimen
V. Choosing the Best Objective Lens for Your Application
VI. Acquiring Digital Images Over Time
VII.
VIII.
IX. Conclusion
References
8.
Edward H. Hinchdiffe and Greenfield Sluder
I. Introduction
II. The Black and White Video Signal
III. Adjusting the Camera and Video Monitor
IV. Practical Aspects of Coordinately Adjusting Camera and Monitor
References
9.
Joshua
I. Introduction
II. Measuring Gray-Level Information
III. Camera Settings
IV. Contrast Stretching
V. Camera Versus Image Display Controls
References
10.
Kenneth R. Spring
I. Overview
II. Basic Principles
III. Application of
IV. Future Developments in Imaging Detectors
References
11.
E. D. Salmon, Sidney L. Shaw, Jennifer
Paul S. Maddox, Elaine Yeh, and Kerry Bloom
I. Introduction
II. Design Criteria
III. Microscope Design
IV. Cooled
V. Digital Imaging System
VI. Example Applications
References
12.
Ivan Rasnik, Todd French, Ken
I. Introduction
II. Parameters Characterizing Imaging Devices
III. Specific Imaging Detectors and Features
IV. Conclusions
References
13.
Butch Moomaw
I. Overview
II. Sensitivity
III. Dynamic Range
IV. Spatial Resolution Limits
V. Temporal Resolution
VI. Geometric Distortion
VII.
VIII.
IX. Advanced Technology
References
14.
Noise Reduction, Contrast Enhancement, and Feature Extraction
Richard A. Cardullo and Edward H. Hinchcliffe
I. Introduction
II. Digitization of Images
III. Using Gray Values to Quantify Intensity in the Microscope
IV. Noise Reduction
Contents
V.
VI. Transforms, Convolutions, and Further Uses for Digital Masks
VII.
References
15.
Thomas M. Durcan and Edward H. Hinchcliffe
I. Introduction
II. What Is an Image File?
III. Sampling and Resolution
IV. Bit Depth
V.
VI. Color
VII.
VIII.
IX. Video Files
X. Video Codecs
XL Choosing a Codec
XII.
References
16.
Contrast Light Microscopy
E. D. Salmon and Phong
I. Introduction
II. Basics of DIC Image Formation and Microscope Alignment
III. Basics of Video-Enhanced Contrast
IV. Selection of Microscope and Video Components
V. Test Specimens for Microscope and Video Performance
References
17.
David E. Wolf, Champika Samarasekera, and Jason R. Swedlow
I. So You Want to Do Digital Imaging
II. An Illustrative Example
III. What Is an Image?
IV. What Kind of Quantitative Information Do You Want?
V. Quantitative Microscopy: A Tool
VI. Exercise
Contents
VII.
VIII.
IX. Standard Deviations, Standard Errors of the Mean,
and Other Confusions
X. Dynamic Range, Do Not Waste It
XI. Signal-to-Noise Ratio, S/N, and Signal-to-Background Ratio, S/B
XII.
XIII.
XIV.
XV. Flatfield Correction
XVI.
XVII.
XVIII.
XIX.
XX. Exercise
XXI.
XXII.
XXIII.
XXIV.
XXV.
References
18.
Methods, Analysis, and Corrective Actions
Paul C. Goodwin
I. Introduction
II. Rationale
III. Methods
IV. Discussion
V. Summary
References
19.
Ca"+ and
Nathan O'Connor and
I. Introduction
II. Why Ratio Imaging?
III. Properties of the Indicators BCECF and Fura-2
IV. Calibration of the Fluorescence Signal
V. Components of an Imaging Workstation
VI. Experimental Chamber and
VII.
References
Contents xi
20.
Deconvolution, and Pattern Recognition
Yu-li Wang
I. Introduction
II. Adaptive Noise Filtration
III. Deconvolution
IV. Pattern Recognition-Based Image Restoration
V. Prospectus
References
21.
Jason R. Swedlow
I. Introduction
II. Quantitative Imaging of Biological Samples Using
Fluorescence Microscopy
III. Image Blurring in Biological Samples
IV. Applications for Image Deconvolution
V. Concluding Remarks
References
22.
John M. Murray
I. Introduction
II. Setting Up for Quantitative Imaging
III. Correcting
IV. Limitations to Exact Quantitation
References
23.
Resonance Energy Transfer Microscopy
Richard A. Cardullo
I. Introduction
II. Principles and Basic Methods of FRET
III. FRET Microscopy
IV.
References
xii Contents
24.
Ching- Wei Chang, Dhruv
I. Introduction
II. Fluorophore Excited State Lifetime:
III. Methods for Creating Intracellular Lifetime Maps
IV. FLIM Techniques for Quantitative Cell Biology
V. Summary
References
25.
in Solution and in Living Cells
Dylan A. Bulseco and David E. Wolf
I. Introduction
II. Studying Biological Systems with FCS
III. Designing and Building an FCS Instrument
IV. What Are the Current Commercial Sources of FCS?
V. Summary
References
26.
Rainer
I. Introduction
II. What Is Resolution?
III. Methods Within the Combined Illumination
and Detection Abbe Limit
IV. Methods Circumventing the Abbe Limit
V. A Comment on Near Field Methods and Pendry's Lens
VI. Key Points
References
Index
Volumes in Series |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV022508197 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
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| callnumber-sort | QH 3585 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 3200 WC 5100 |
| ctrlnum | (OCoLC)122282792 (DE-599)BVBBV022508197 |
| dewey-full | 570.282 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570.282 |
| dewey-search | 570.282 |
| dewey-sort | 3570.282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | 3. ed. |
| format | Book |
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| id | DE-604.BV022508197 |
| illustrated | Illustrated |
| index_date | 2024-07-02T17:58:23Z |
| indexdate | 2024-07-09T20:59:08Z |
| institution | BVB |
| isbn | 9780123740250 0123740258 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015715151 |
| oclc_num | 122282792 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 DE-29 |
| owner_facet | DE-355 DE-BY-UBR DE-20 DE-29 |
| physical | XVII, 608 S., [9] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Digital microscopy ed. by Greenfield Sluder ... 3. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2007 XVII, 608 S., [9] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 81 Digitale technieken gtt Microscopie gtt Traitement d'images - Techniques numériques Vidéomicroscopie Image Processing, Computer-Assisted instrumentation Image Processing, Computer-Assisted methods Image processing Digital techniques Microscopy instrumentation Microscopy methods Video microscopy Digitalkamera (DE-588)4519830-5 gnd rswk-swf Bildanalyse (DE-588)4145391-8 gnd rswk-swf Videomikroskopie (DE-588)4367875-0 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Videomikroskopie (DE-588)4367875-0 s Bildanalyse (DE-588)4145391-8 s DE-604 Mikroskopie (DE-588)4039238-7 s Digitalkamera (DE-588)4519830-5 s b DE-604 Sluder, Greenfield Sonstige oth Methods in cell biology 81 (DE-604)BV002534878 81 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015715151&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Digital microscopy Methods in cell biology Digitale technieken gtt Microscopie gtt Traitement d'images - Techniques numériques Vidéomicroscopie Image Processing, Computer-Assisted instrumentation Image Processing, Computer-Assisted methods Image processing Digital techniques Microscopy instrumentation Microscopy methods Video microscopy Digitalkamera (DE-588)4519830-5 gnd Bildanalyse (DE-588)4145391-8 gnd Videomikroskopie (DE-588)4367875-0 gnd Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4519830-5 (DE-588)4145391-8 (DE-588)4367875-0 (DE-588)4039238-7 |
| title | Digital microscopy |
| title_auth | Digital microscopy |
| title_exact_search | Digital microscopy |
| title_exact_search_txtP | Digital microscopy |
| title_full | Digital microscopy ed. by Greenfield Sluder ... |
| title_fullStr | Digital microscopy ed. by Greenfield Sluder ... |
| title_full_unstemmed | Digital microscopy ed. by Greenfield Sluder ... |
| title_short | Digital microscopy |
| title_sort | digital microscopy |
| topic | Digitale technieken gtt Microscopie gtt Traitement d'images - Techniques numériques Vidéomicroscopie Image Processing, Computer-Assisted instrumentation Image Processing, Computer-Assisted methods Image processing Digital techniques Microscopy instrumentation Microscopy methods Video microscopy Digitalkamera (DE-588)4519830-5 gnd Bildanalyse (DE-588)4145391-8 gnd Videomikroskopie (DE-588)4367875-0 gnd Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Digitale technieken Microscopie Traitement d'images - Techniques numériques Vidéomicroscopie Image Processing, Computer-Assisted instrumentation Image Processing, Computer-Assisted methods Image processing Digital techniques Microscopy instrumentation Microscopy methods Video microscopy Digitalkamera Bildanalyse Videomikroskopie Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015715151&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT sludergreenfield digitalmicroscopy |