Characterization of virulence genes in Salmonella pathogenicity island 4:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | II, 144 S. Ill., graph. Darst. |
Staff View
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| 245 | 1 | 0 | |a Characterization of virulence genes in Salmonella pathogenicity island 4 |c von Roman G. Gerlach |
| 264 | 1 | |c 2007 | |
| 300 | |a II, 144 S. |b Ill., graph. Darst. | ||
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| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2007 | ||
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Record in the Search Index
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| adam_text | Table of contents
1. INTRODUCTION 1
1.1. Salmonella 1
1.2. Secretion systems in Gram negative bacteria 2
1.2.1. The general secretory pathway (GSP) 2
1.2.2. Type I 3
1.2.3. Type II 4
1.2.4. Type III 4
1.2.5. Type IV 6
1.2.6. TypeV 7
1.2.7. Chaperone usher (CU) pathway 8
1.2.8. Other secretion pathways 8
1.3. Salmonella virulence factors 10
1.3.1. SPI1 10
1.3.2. SPI2 11
1.3.3. SPI3 12
1.3.4. SPI4 13
1.3.5. SPI5 13
1.3.6. SPI9 14
1.3.7. Other SPI 14
1.4. Bacterial adhesion factors 15
1.4.1. Fimbrial adhesins 15
1.4.1.1. TypelandPpili 16
1.4.1.2. Afa/Dr adhesins 17
1.4.1.3. CSpili 18
1.4.1.4. Type IV pili 18
1.4.1.5. Curli 19
1.4.2. Non fimbrial/afimbrial adhesins 20
1.4.2.1. Autotransported adhesins 20
1.4.2.2. Integral outer membrane adhesins 21
1.4.2.3. Secreted adhesins 22
1.4.2.4. Adhesins associated with biofilm formation 23
1.5. Analysis of virulence gene regulation in Salmonella 24
1.5.1. The SPI1 regulon 24
1.5.2. SPI4 regulation 26
1.5.3. SPI2 gene regulation 27
1.5.4. Tools to analyze gene regulation 28
1.5.4.1. Use of reporter fusions 28
1.5.4.2. Generation of reporter fusions 29
1.5.4.3. Red recombinase based methods 30
1.5.4.4. Frequently used reporter genes 31
2. AIM OF THE WORK 34
3. RESULTS AND PUBLICATIONS 35
I
3.1. Rapid engineering of bacterial reporter gene fusions using Red recombination 35
3.2. Salmonella Pathogenicity Island 4 mediated adhesion is co regulated with invasion
genes in Salmonella enterica 52
3.3. Salmonella Pathogenicity Island 4 encodes a giant non fimbrial adhesin and the
cognate type I secretion system 70
3.4. Cooperation of SPI4 and SPI1 is required for invasion of polarized epithelial cells. 100
3.5. Control of surface localization of the non fimbrial adhesin SiiE 103
3.6. Contribution of co authors 106
4. DISCUSSION 107
4.1. A new rationale for generating reporter fusions 107
4.2. SPI4 is co regulated with invasion genes 109
4.3. The virulence function of Salmonella Pathogenicity Island 4 113
4.4. Conclusions 119
5. SUMMARY/ZUSAMMENFASSUNG 122
6. REFERENCES 125
7. ABBREVIATIONS 137
8. LEBENSLAUF 141
9. DANKSAGUNG 144
II
|
| adam_txt |
Table of contents
1. INTRODUCTION 1
1.1. Salmonella 1
1.2. Secretion systems in Gram negative bacteria 2
1.2.1. The general secretory pathway (GSP) 2
1.2.2. Type I 3
1.2.3. Type II 4
1.2.4. Type III 4
1.2.5. Type IV 6
1.2.6. TypeV 7
1.2.7. Chaperone usher (CU) pathway 8
1.2.8. Other secretion pathways 8
1.3. Salmonella virulence factors 10
1.3.1. SPI1 10
1.3.2. SPI2 11
1.3.3. SPI3 12
1.3.4. SPI4 13
1.3.5. SPI5 13
1.3.6. SPI9 14
1.3.7. Other SPI 14
1.4. Bacterial adhesion factors 15
1.4.1. Fimbrial adhesins 15
1.4.1.1. TypelandPpili 16
1.4.1.2. Afa/Dr adhesins 17
1.4.1.3. CSpili 18
1.4.1.4. Type IV pili 18
1.4.1.5. Curli 19
1.4.2. Non fimbrial/afimbrial adhesins 20
1.4.2.1. Autotransported adhesins 20
1.4.2.2. Integral outer membrane adhesins 21
1.4.2.3. Secreted adhesins 22
1.4.2.4. Adhesins associated with biofilm formation 23
1.5. Analysis of virulence gene regulation in Salmonella 24
1.5.1. The SPI1 regulon 24
1.5.2. SPI4 regulation 26
1.5.3. SPI2 gene regulation 27
1.5.4. Tools to analyze gene regulation 28
1.5.4.1. Use of reporter fusions 28
1.5.4.2. Generation of reporter fusions 29
1.5.4.3. Red recombinase based methods 30
1.5.4.4. Frequently used reporter genes 31
2. AIM OF THE WORK 34
3. RESULTS AND PUBLICATIONS 35
I
3.1. Rapid engineering of bacterial reporter gene fusions using Red recombination 35
3.2. Salmonella Pathogenicity Island 4 mediated adhesion is co regulated with invasion
genes in Salmonella enterica 52
3.3. Salmonella Pathogenicity Island 4 encodes a giant non fimbrial adhesin and the
cognate type I secretion system 70
3.4. Cooperation of SPI4 and SPI1 is required for invasion of polarized epithelial cells. 100
3.5. Control of surface localization of the non fimbrial adhesin SiiE 103
3.6. Contribution of co authors 106
4. DISCUSSION 107
4.1. A new rationale for generating reporter fusions 107
4.2. SPI4 is co regulated with invasion genes 109
4.3. The virulence function of Salmonella Pathogenicity Island 4 113
4.4. Conclusions 119
5. SUMMARY/ZUSAMMENFASSUNG 122
6. REFERENCES 125
7. ABBREVIATIONS 137
8. LEBENSLAUF 141
9. DANKSAGUNG 144
II |
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| index_date | 2024-07-02T17:51:54Z |
| indexdate | 2024-07-09T20:58:45Z |
| institution | BVB |
| language | English |
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| publishDate | 2007 |
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| spelling | Gerlach, Roman G. 1978- Verfasser (DE-588)133175723 aut Characterization of virulence genes in Salmonella pathogenicity island 4 von Roman G. Gerlach 2007 II, 144 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2007 Pathogenität (DE-588)4205990-2 gnd rswk-swf Salmonella enterica (DE-588)4511561-8 gnd rswk-swf Virulenzfaktor (DE-588)4354782-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Salmonella enterica (DE-588)4511561-8 s Virulenzfaktor (DE-588)4354782-5 s Pathogenität (DE-588)4205990-2 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015698261&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gerlach, Roman G. 1978- Characterization of virulence genes in Salmonella pathogenicity island 4 Pathogenität (DE-588)4205990-2 gnd Salmonella enterica (DE-588)4511561-8 gnd Virulenzfaktor (DE-588)4354782-5 gnd |
| subject_GND | (DE-588)4205990-2 (DE-588)4511561-8 (DE-588)4354782-5 (DE-588)4113937-9 |
| title | Characterization of virulence genes in Salmonella pathogenicity island 4 |
| title_auth | Characterization of virulence genes in Salmonella pathogenicity island 4 |
| title_exact_search | Characterization of virulence genes in Salmonella pathogenicity island 4 |
| title_exact_search_txtP | Characterization of virulence genes in Salmonella pathogenicity island 4 |
| title_full | Characterization of virulence genes in Salmonella pathogenicity island 4 von Roman G. Gerlach |
| title_fullStr | Characterization of virulence genes in Salmonella pathogenicity island 4 von Roman G. Gerlach |
| title_full_unstemmed | Characterization of virulence genes in Salmonella pathogenicity island 4 von Roman G. Gerlach |
| title_short | Characterization of virulence genes in Salmonella pathogenicity island 4 |
| title_sort | characterization of virulence genes in salmonella pathogenicity island 4 |
| topic | Pathogenität (DE-588)4205990-2 gnd Salmonella enterica (DE-588)4511561-8 gnd Virulenzfaktor (DE-588)4354782-5 gnd |
| topic_facet | Pathogenität Salmonella enterica Virulenzfaktor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015698261&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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