Flow cytometry: first principles
Saved in:
| Format: | Electronic eBook |
|---|---|
| Language: | English |
| Published: |
New York [u.a.]
Wiley-Liss
2001
|
| Edition: | 2. ed. |
| Subjects: | |
| Online Access: | FRO01 TUM01 UBR01 Volltext Inhaltsverzeichnis |
| ISBN: | 0471223948 0471382248 9780471223948 9780471382249 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Preface xi
Acknowledgments (First Edition) xv
Acknowledgments (Second Edition) xvii
1 The Past as Prologue 1
2 Setting the Scene 11
3 Instrumentation: Into the Black Box 15
Illumination of the Stream 16
Centering Cells in the Illuminating Beam 18
Detection of Signals from Cells 25
Electronics 31
4 Information: Harnessing the Data 41
Data Storage 41
Data Analysis 43
5 Seeing the Light: Lasers, Fluorochromes, and
Filters 59
General Theory 59
Lasers 61
Fluorochromes 65
Partitioning the Signal with Lenses, Filters, and
Mirrors 72
Spectral Compensation 76
vii
viii Flow Cytometry
6 Cells from Without: Leukocytes, Surface Proteins, and
the Strategy of Gating 81
Cells from Blood 82
Staining for Surface Markers 87
Controls 90
Quantitation 95
Sensitivity 98
The Strategy of Gating 99
Gating on Fluorescence 108
7 Cells from Within: Intracellular Proteins 115
Methods for Permeabilizing Cells 116
Examples of Intracellular Staining 118
8 Cells from Within: DNA in Life and Death 123
Fluorochromes for DNA Analysis 123
Ploidy 125
Cell Cycle Analysis 131
Two Color Analysis for DNA and Another
Parameter 142
Chromosomes 147
Apoptosis 150
Necrosis 154
9 The Sorting of Cells 159
Sorting Theory 159
Characterization of Sorted Cells 166
Alternative Methods for Sorting 1 70
The Condition of Cells After Sorting 1 72
10 Disease and Diagnosis: The Clinical Laboratory 175
The Hematology Laboratory 1 78
The Pathology Laboratory 186
Solid Organ Transplantation 189
Comments 192
Contents ix
11 Out of the Mainstream: Research Frontiers 195
Functional Assays 196
The Aquatic Environment 202
Reporter Molecules 207
Microbiology 211
Molecular Biology 213
Multiplex Cytometry for Soluble Analytes 218
Reproductive Technology 220
12 Flowing On: The Future 225
General References 229
Glossary 235
Figure Credits 257
Index 263
|
| adam_txt |
Contents
Preface xi
Acknowledgments (First Edition) xv
Acknowledgments (Second Edition) xvii
1 The Past as Prologue 1
2 Setting the Scene 11
3 Instrumentation: Into the Black Box 15
Illumination of the Stream 16
Centering Cells in the Illuminating Beam 18
Detection of Signals from Cells 25
Electronics 31
4 Information: Harnessing the Data 41
Data Storage 41
Data Analysis 43
5 Seeing the Light: Lasers, Fluorochromes, and
Filters 59
General Theory 59
Lasers 61
Fluorochromes 65
Partitioning the Signal with Lenses, Filters, and
Mirrors 72
Spectral Compensation 76
vii
viii Flow Cytometry
6 Cells from Without: Leukocytes, Surface Proteins, and
the Strategy of Gating 81
Cells from Blood 82
Staining for Surface Markers 87
Controls 90
Quantitation 95
Sensitivity 98
The Strategy of Gating 99
Gating on Fluorescence 108
7 Cells from Within: Intracellular Proteins 115
Methods for Permeabilizing Cells 116
Examples of Intracellular Staining 118
8 Cells from Within: DNA in Life and Death 123
Fluorochromes for DNA Analysis 123
Ploidy 125
Cell Cycle Analysis 131
Two Color Analysis for DNA and Another
Parameter 142
Chromosomes 147
Apoptosis 150
Necrosis 154
9 The Sorting of Cells 159
Sorting Theory 159
Characterization of Sorted Cells 166
Alternative Methods for Sorting 1 70
The Condition of Cells After Sorting 1 72
10 Disease and Diagnosis: The Clinical Laboratory 175
The Hematology Laboratory 1 78
The Pathology Laboratory 186
Solid Organ Transplantation 189
Comments 192
Contents ix
11 Out of the Mainstream: Research Frontiers 195
Functional Assays 196
The Aquatic Environment 202
Reporter Molecules 207
Microbiology 211
Molecular Biology 213
Multiplex Cytometry for Soluble Analytes 218
Reproductive Technology 220
12 Flowing On: The Future 225
General References 229
Glossary 235
Figure Credits 257
Index 263 |
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| spelling | Flow cytometry first principles Alice Longobardi Givan 2. ed. New York [u.a.] Wiley-Liss 2001 txt rdacontent c rdamedia cr rdacarrier Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd rswk-swf Durchflusscytometrie (DE-588)4226628-2 s DE-604 Givan, Alice L. Sonstige oth https://onlinelibrary.wiley.com/doi/book/10.1002/0471223948 Verlag Volltext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015623944&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Flow cytometry first principles Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd |
| subject_GND | (DE-588)4226628-2 |
| title | Flow cytometry first principles |
| title_auth | Flow cytometry first principles |
| title_exact_search | Flow cytometry first principles |
| title_exact_search_txtP | Flow cytometry first principles |
| title_full | Flow cytometry first principles Alice Longobardi Givan |
| title_fullStr | Flow cytometry first principles Alice Longobardi Givan |
| title_full_unstemmed | Flow cytometry first principles Alice Longobardi Givan |
| title_short | Flow cytometry |
| title_sort | flow cytometry first principles |
| title_sub | first principles |
| topic | Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd |
| topic_facet | Flow Cytometry methods Flow cytometry Durchflusscytometrie |
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