Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer:
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| Main Author: | |
|---|---|
| Format: | Thesis Electronic Software eBook |
| Language: | English |
| Published: |
2005
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 1 CD-ROM; 12 cm 12 cm |
Staff View
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| 100 | 1 | |a Bhojwani, Sanjay |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |c eingereicht von Sanjay Bhojwani |
| 264 | 1 | |c 2005 | |
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Record in the Search Index
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| adam_text |
TABLE
OF
CONTENTS
1
INTRODUCTION
1
2
REVIEW
OF
LITERATURE
9
2.1
MATURATION,
ENUCLEATION,
ACTIVATION
----------------------------
9
2.1.1
MEIOTIC
MATURATION
----------------------------
9
2.1.2
ENUCLEATION
AND
RECONSTRUCTION
---------------------------
11
2.1.3
ACTIVATION
12
2.2
DONOR
CELLS
14
2.2.1
SELECTION
OF
DONOR
CELLS
14
2.2.2
DONOR
CELL
CYCLE
STAGES
15
2.2.3
CELL
LINE
CLONING
EFFICIENCY
---------------------------
18
2.3
GENOMIC
ACTIVATION
&
IMPRINTING
----------------------------
18
2.3.1
GENOMIC
ACTIVATION
AND
NUCLEAR
REPROGRAMMING
----------------------------
18
2.3.2
EPIGENESIS
AND
IMPRINTING
---------------------------
20
2.3.3
DNA
METHYLATION
22
2.3.4
X-CHROMOSOME
INACTIVATION
----------------------------
24
2.3.5
LONG-LASTING
EFFECTS
OF
EPIGENETIC ALTERATIONS
24
2.4
MITOCHONDRIA
25
2.4.1
MITOCHONDRIAL
INHERITANCE
----------------------------
25
2.4.2
ALTERATIONS
TO
THE
MTDNA
----------------------------
26
2.4.3
NUCLEAR-MITOCHONDRIAL
INTERACTIONS
---------------------------
27
2.5
SPECIFIC ISSUES
28
2.5.1
CLONING
AND
TELOMERES
28
2.5.2
INTERSPECIES
NUCLEAR
TRANSFER
----------------------------
30
2.5.3
CLONING
AND
RISK
ASSESSMENT
----------------------------
31
2.5.4
ETHICAL
CONCERNS
31
2.6
CYTOSKELETON
&
CELL-TO-CELL
CONTACTS
----------------------------
33
2.6.1
CYTOSKELETON
33
2.6.2
GAP
JUNCTIONS
34
3
MATERIALS
AND METHODS
---------------------------
36
3.1
NUCLEAR
DONOR
CELLS
-
CELL
CULTURE
AND
PASSAGES
---------
36
3.2
OOCYTE
COLLECTION
AND
IN
VITRO
MATURATION
(IVM)
---------
36
3.3
NUCLEAR
TRANSFER
(NT)
-HMCYY
TECHNIQUE
37
3.4
DEVELOPMENTAL
POTENTIAL
OF
ZONA-FREE
CLONED
BOVINE
EMBRYOS
-
40
3.5
BLASTOCYST TRANSFER,
PREGNANCY
MONITORING
AND
BIRTH
---------
40
3.6
IN
VITRO
EMBRYO
PRODUCTION
(IVP)
41
3.7
PRODUCTION
OF
IN
VIVO
EMBRYOS
42
3.8
ULTRASTRUCTURAL
ANALYSIS
42
3.9
ONE
DIMENSIONAL
GEL
ELECTROPHORESIS
43
3.10
WESTERN
BLOTTING
AND
IMMUNODETECTION
43
3.11
IMMUNOHISTOCHEMISTRY
OF
EMBRYOS
44
3.12
CONFOCAL LASER
SCANNING
MICROSCOPY
46
3.13
CLUSTER
ANALYSIS
(WINSTAT
&
"
LABVIEW
"
)
47
3.14
STATISTICAL
ANALYSIS
48
4
RESULTS
---------------------------
49
4.1
CELL
CYCLE
ANALYSIS
OF
DONOR
CELLS
49
4.2
SUITABILITY
OF
DONOR
CELLS
50
4.3
DEVELOPMENT
COMPETENCE
OF
CLONED
EMBRYOS
-----------
51
4.4
EMBRYO
TRANSFERS
AND
BIRTH
OF
THE
CLONED
CALF
------------
52
4.5
CLONED
BLASTOCYSTS
54
4.6
CONFOCAL
ASSESSMENT
OF
RECONSTRUCTED
EMBRYOS
------------
55
4.7
"
LAB
VIEW
"
ANALYSIS
60
4.8
CLUSTER
ANALYSIS
(WINSTAT)
65
4.9
WESTERN
BLOT ANALYSIS
68
4.10
ELECTRON
MICROSCOPY
69
II
5
DISCUSSION
----------------------------
72
5.1
SUITABILITY
OF
DONOR
CELLS
72
5.2
SUITABILITY
OF
OOCYTES
74
5.3
ACTIVATION
AND
DEVELOPMENTAL
BLOCK
76
5.4
NT
BLASTOCYST
RATES
AND
PREGNANCIES,
EPIGENESIS
----------
78
5.5
CYTOSKELETAL
PROTEIN
DISTRIBUTION
81
5.6
CONNEXIN
43
AND
GAP
JUNCTIONS
85
5.7
TIGHT
JUNCTIONS,
CONTACT
POINTS
(CPS)
88
5.8
GENERAL
REMARKS
89
6
SUMMARY
91
7
ZUSAMMENFASSUNG
93
8
LITERATURE
CITED
9
APPENDIX
10
ACKNOWLEDGEMENT
III |
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| indexdate | 2024-08-24T00:11:54Z |
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| spelling | Bhojwani, Sanjay Verfasser aut Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer eingereicht von Sanjay Bhojwani 2005 1 CD-ROM; 12 cm 12 cm txt rdacontent c rdamedia cd rdacarrier Leipzig, Univ., Diss., 2005 Embryotransfer (DE-588)4113430-8 gnd rswk-swf Rind (DE-588)4050061-5 gnd rswk-swf Zellskelett (DE-588)4121944-2 gnd rswk-swf Kerntransplantation (DE-588)4521494-3 gnd rswk-swf Zellkommunikation (DE-588)4136131-3 gnd rswk-swf Zona pellucida (DE-588)4127311-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rind (DE-588)4050061-5 s Embryotransfer (DE-588)4113430-8 s Zona pellucida (DE-588)4127311-4 s Kerntransplantation (DE-588)4521494-3 s Zellskelett (DE-588)4121944-2 s Zellkommunikation (DE-588)4136131-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015611594&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bhojwani, Sanjay Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer Embryotransfer (DE-588)4113430-8 gnd Rind (DE-588)4050061-5 gnd Zellskelett (DE-588)4121944-2 gnd Kerntransplantation (DE-588)4521494-3 gnd Zellkommunikation (DE-588)4136131-3 gnd Zona pellucida (DE-588)4127311-4 gnd |
| subject_GND | (DE-588)4113430-8 (DE-588)4050061-5 (DE-588)4121944-2 (DE-588)4521494-3 (DE-588)4136131-3 (DE-588)4127311-4 (DE-588)4113937-9 |
| title | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| title_auth | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| title_exact_search | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| title_exact_search_txtP | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| title_full | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer eingereicht von Sanjay Bhojwani |
| title_fullStr | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer eingereicht von Sanjay Bhojwani |
| title_full_unstemmed | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer eingereicht von Sanjay Bhojwani |
| title_short | Investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| title_sort | investigations of cellular communication and cytoskeleton in bovine embryos after zona free somatic cell nuclear transfer |
| topic | Embryotransfer (DE-588)4113430-8 gnd Rind (DE-588)4050061-5 gnd Zellskelett (DE-588)4121944-2 gnd Kerntransplantation (DE-588)4521494-3 gnd Zellkommunikation (DE-588)4136131-3 gnd Zona pellucida (DE-588)4127311-4 gnd |
| topic_facet | Embryotransfer Rind Zellskelett Kerntransplantation Zellkommunikation Zona pellucida Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015611594&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bhojwanisanjay investigationsofcellularcommunicationandcytoskeletoninbovineembryosafterzonafreesomaticcellnucleartransfer |