Cellular electron microscopy:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Series: | Methods in cell biology
79 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXVII, 850 S., [14] Bl. Ill., graph. Darst. |
| ISBN: | 9780123706478 0123706475 |
Staff View
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| adam_text | CONTENTS
Contributors
Introduction
PART I Preparing Cells for Electron Microscopy
1.
Cryoimmobilization of Oils
Jacques Dubochet
I. Introduction
II. Freezing Water
III. Biological Material
IV. Freezing Biological Material
References
2.
Selected Model Systems
Kent McDonald
I. Introduction
II. Equipment and Materials
III. General Rules for Loading Samples for HPF
IV. Methods for Specific Organisms
V. Postfreezing Processing
VI. Summary
References
3.
Michael IV. Hess
I. Introduction
II. Rationale
III. Methods
IV. Materials
Contents
V.
VI. Concluding Remarks
References
4.
Embryos in Mitosis
Thomas
and Kent McDonald
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
PART II Imaging Fixed Cells in Three Dimensions
5.
Mutant and Wild-Type Structures with Electron Tomography
Eileen T, O Toole, Ihomas II. Giddings
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
VI. Summary
References
6.
Architecture by Electron Tomography
ƒ.
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
VI. Summary I55
References ](,5
Contents
7. Electron
Citarles
I. Introduction
II. Rationale
III. Methods and Materials
IV. Discussion
V. Summary
References
8.
Area Cellular Tomography
Brad]. Marsh
I. Introduction and Rationale
II. Methods and Materials
III. Discussion
IV. Summary
References
9.
Marisa
I. Introduction
II. Rationale
III. Materials and Methods
IV. Discussion
V. Summary
References
10.
Christopher
Dudley S. Finch, and Michael H. B. Stowell
I. Introduction and Rationale
II.
III. 11T and the Synapse
IV. Types of Neurons and Synapses
V. Sources of Neurons for Structural Studies
VI. Methods for Assessing Neuron and Synapse Integrity
VII.
VIII.
IX. Stimulation-Dependent Changes in Synaptic Structure
X. The Future of EM Tomography and Synapses
XI. Conclusions
References
Contents
11.
of Chromosome Alignment on the Mitotic Spindle
Bruce
I. Introduction
II. Basic
III. Organization of MTs in the Mitotic
IV. Deciphering Mechanisms of Chromosome Alignment and
K-Fiber Formation Using (Correlative Microscopy
V. Toward a Molecular Understanding of Kinetochore Architecture
VI. Beyond Morphology: Use of Electron Tomography to Model
Kinetochore (Control of Ml Dynamics
VII.
References
12.
ľatyana
I. Introduction
II. Understanding the Mechanisms of Leading Edge Protrusion:
Complementary Roles of LM and EM
III. Platinum Replica EM of the Leading Edge Cytoskeleton
IV. Identification of Cytoskeletal Components in Platinum
Replica Preparations
References
13.
Kenneth A
I. Introduction and Overview
II. Preparing the Specimen
III. Data Collection and Tomogram (Calculation
IV. Identifying Similar Structures in an Ensemble
V. Building Atomic Models
VI. Relating Class Averages to the Specimen
VII.
VIII.
IX. Summary
References
Contents
PART III Imaging Frozen-Hydrated Cells and Cell Parts
14.
Pcijun Zhang, Robert M. Wets, Peter J. Peters, and Sriram Subramaniatn
I. Introduction
II. Analysis of Tsr Assemblies in Membrane Extracts
III. Electron Tomography of Fixed, Cryosectioned
IV. Cryo-Electron Microscopy of Frozen-I lydrated Sections of
V. Structure of Chemotaxis Receptors Using Cryo-Electron Tomography
VI. Summary
References
15.
Jacques Dubochet,
Ashtaf Al-Atnoudi, and
I. Introduction
II. Conventional Versus Cryo-Electron Microscopy: A Comparison
III. Illustration: Two Pitfalls
References
16.
Excitation- -Contraction Coupling Junction
Irina
I. Introduction
II. Single-Particle Cryo-EM Methodology
III.
IV. Spatial Arrangement of DHPRs and RyRs in Triad Junctions
V. Summary
References
17.
Andreas lioenger and
I. Introduction
II. Rationale
III. Methods
IV. Discussion
References
Contents
18.
Yífan
I. Introduction
II. Rationale
III. Methods
IV. Results
V. Discussion
VI. Summary
References
PART IV Localizing Macromolecules in Cells
19. 3D
Mary K. Morplieii
I. Introduction
II. Rationale for the Various Approaches to Immuno-EM
HI. Methods for Immuno-EM
IV. Comparison of the Methods
V. Summary
References
20.
Annegret Pelcheti-Mattheivs
I. Introduction
II. Analysis of Virus Assembly on Plastic Sections
III. Immunolabcling of Ultrathin Ciyosections: Applications
of the Tokuyasu Technique to Study Virus Assembly
IV. Future Developments
V. Summary and Conclusions
References
21.
Mark
I. Introduction
II. Methods
III. Results and Discussion
References
Contents
22.
Microscopy to Electron Tomography
T. Cheutin, C.
II. Kaplan,
I. Introduction
II.
III. Applications
IV. Discussion
References
23.
Gina
Guido
I. Introduction
II. How Do LM and EM Complement Each Other?
III. Fluorescence Photooxidation
IV. Enzymatic-Based Methods
V. Particle-Based Methods for Protein Localization
VI. Concluding Remarks
References
24.
Richard D.
I. Introduction
II. General Principles
III. Applications
IV. Summary and
References
25.
Christoph Best, Stephan Nicki ll,
I. Introduction
II. Template Matching
III. The Missing-Wedge Problem
IV. Applications
V. Conclusions
References
Contents
PART V Aspects of Data Collection and Analysis
26.
Tomography of Thick, Selectively Stained Biological Samples
James C.
Ying Z. Jones, Masako
and Mark H. Ellisman
I. Introduction
II. Introduction to MPL Imaging
III. Detailed Description of MPL Tomography
IV. Techniques
V. Results
VI. Conclusions
References
27.
Paid
I. Introduction: Assessing the Role of Image Collection in Cellular
Electron Microscopy
II. Applications: The Importance and Meaning ot SSNR in
Cell Biology Applications
III. Optimization of Detectors and of the Detection Process
for Maximization of SSNR
IV. Detectors: Proven Technologies
V. Detectors: Experimental Technologies
VI. Discussion
VII.
References
28.
X^uyen-Hnu
Philippe Leblanc,
Amia-Calare
I. Introduction
II. Background and Rationale
III. Description of the Direct Detection Detector
!V. Detector Characterization
V. Radiation Damage
VI. Discussion 7^C
References
Contents
29.
Friedrich Förster and Reiner Heger!
I. Introduction
II. Tomography and Its Increase in Resolution by Averaging
III. Coherent Averaging of
IV. Applications of Tomogram Averaging
V. Conclusion and Outlook
References
30.
Kristinu
I. Introduction
II. Problem Formulation
III. Challenges in Cellular Tomography Segmentation
IV. Important Considerations for Segmentation Methods
V. How
VI. Segmentation Methods for Cellular Tomography
VII.
VIII.
IX.
References
31.
Maryaim
Heather files, ami
I. Introduction HOtl
II. The OH Centered Database Project
III. Looking Ahead
References
Index
Volumes in Series
|
| adam_txt |
CONTENTS
Contributors
Introduction
PART I Preparing Cells for Electron Microscopy
1.
Cryoimmobilization of Oils
Jacques Dubochet
I. Introduction
II. Freezing Water
III. Biological Material
IV. Freezing Biological Material
References
2.
Selected Model Systems
Kent McDonald
I. Introduction
II. Equipment and Materials
III. General Rules for Loading Samples for HPF
IV. Methods for Specific Organisms
V. Postfreezing Processing
VI. Summary
References
3.
Michael IV. Hess
I. Introduction
II. Rationale
III. Methods
IV. Materials
Contents
V.
VI. Concluding Remarks
References
4.
Embryos in Mitosis
Thomas
and Kent McDonald
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
PART II Imaging Fixed Cells in Three Dimensions
5.
Mutant and Wild-Type Structures with Electron Tomography
Eileen T, O'Toole, 'Ihomas II. Giddings
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
VI. Summary
References
6.
Architecture by Electron Tomography
ƒ.
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
VI. Summary I55
References ](,5
Contents
7. Electron
Citarles
I. Introduction
II. Rationale
III. Methods and Materials
IV. Discussion
V. Summary
References
8.
Area Cellular Tomography
Brad]. Marsh
I. Introduction and Rationale
II. Methods and Materials
III. Discussion
IV. Summary
References
9.
Marisa
I. Introduction
II. Rationale
III. Materials and Methods
IV. Discussion
V. Summary
References
10.
Christopher
Dudley S. Finch, and Michael H. B. Stowell
I. Introduction and Rationale
II.
III. 11T and the Synapse
IV. Types of Neurons and Synapses
V. Sources of Neurons for Structural Studies
VI. Methods for Assessing Neuron and Synapse Integrity
VII.
VIII.
IX. Stimulation-Dependent Changes in Synaptic Structure
X. The Future of EM Tomography and Synapses
XI. Conclusions
References
Contents
11.
of Chromosome Alignment on the Mitotic Spindle
Bruce
I. Introduction
II. Basic
III. Organization of MTs in the Mitotic
IV. Deciphering Mechanisms of Chromosome Alignment and
K-Fiber Formation Using (Correlative Microscopy
V. Toward a Molecular Understanding of Kinetochore Architecture
VI. Beyond Morphology: Use of Electron Tomography to Model
Kinetochore (Control of Ml Dynamics
VII.
References
12.
'ľatyana
I. Introduction
II. Understanding the Mechanisms of Leading Edge Protrusion:
Complementary Roles of LM and EM
III. Platinum Replica EM of the Leading Edge Cytoskeleton
IV. Identification of Cytoskeletal Components in Platinum
Replica Preparations
References
13.
Kenneth A
I. Introduction and Overview
II. Preparing the Specimen
III. Data Collection and Tomogram (Calculation
IV. Identifying Similar Structures in an Ensemble
V. Building Atomic Models
VI. Relating Class Averages to the Specimen
VII.
VIII.
IX. Summary
References
Contents
PART III Imaging Frozen-Hydrated Cells and Cell Parts
14.
Pcijun Zhang, Robert M. Wets, Peter J. Peters, and Sriram Subramaniatn
I. Introduction
II. Analysis of Tsr Assemblies in Membrane Extracts
III. Electron Tomography of Fixed, Cryosectioned
IV. Cryo-Electron Microscopy of Frozen-I lydrated Sections of
V. Structure of Chemotaxis Receptors Using Cryo-Electron Tomography
VI. Summary
References
15.
Jacques Dubochet,
Ashtaf Al-Atnoudi, and
I. Introduction
II. Conventional Versus Cryo-Electron Microscopy: A Comparison
III. Illustration: Two Pitfalls
References
16.
Excitation- -Contraction Coupling Junction
Irina
I. Introduction
II. Single-Particle Cryo-EM Methodology
III.
IV. Spatial Arrangement of DHPRs and RyRs in Triad Junctions
V. Summary
References
17.
Andreas lioenger and
I. Introduction
II. Rationale
III. Methods
IV. Discussion
References
Contents
18.
Yífan
I. Introduction
II. Rationale
III. Methods
IV. Results
V. Discussion
VI. Summary
References
PART IV Localizing Macromolecules in Cells
19. 3D
Mary K. Morplieii'
I. Introduction
II. Rationale for the Various Approaches to Immuno-EM
HI. Methods for Immuno-EM
IV. Comparison of the Methods
V. Summary
References
20.
Annegret Pelcheti-Mattheivs
I. Introduction
II. Analysis of Virus Assembly on Plastic Sections
III. Immunolabcling of Ultrathin Ciyosections: Applications
of the Tokuyasu Technique to Study Virus Assembly
IV. Future Developments
V. Summary and Conclusions
References
21.
Mark
I. Introduction
II. Methods
III. Results and Discussion
References
Contents
22.
Microscopy to Electron Tomography
T. Cheutin, C.
II. Kaplan,
I. Introduction
II.
III. Applications
IV. Discussion
References
23.
Gina
Guido
I. Introduction
II. How Do LM and EM Complement Each Other?
III. Fluorescence Photooxidation
IV. Enzymatic-Based Methods
V. Particle-Based Methods for Protein Localization
VI. Concluding Remarks
References
24.
Richard D.
I. Introduction
II. General Principles
III. Applications
IV. Summary and
References
25.
Christoph Best, Stephan Nicki'll,
I. Introduction
II. Template Matching
III. The Missing-Wedge Problem
IV. Applications
V. Conclusions
References
Contents
PART V Aspects of Data Collection and Analysis
26.
Tomography of Thick, Selectively Stained Biological Samples
James C.
Ying Z. Jones, Masako
and Mark H. Ellisman
I. Introduction
II. Introduction to MPL Imaging
III. Detailed Description of MPL Tomography
IV. Techniques
V. Results
VI. Conclusions
References
27.
Paid
I. Introduction: Assessing the Role of Image Collection in Cellular
Electron Microscopy
II. Applications: The Importance and Meaning ot SSNR in
Cell Biology Applications
III. Optimization of Detectors and of the Detection Process
for Maximization of SSNR
IV. Detectors: Proven Technologies
V. Detectors: Experimental Technologies
VI. Discussion
VII.
References
28.
X^uyen-Hnu
Philippe Leblanc,
Amia-Calare
I. Introduction
II. Background and Rationale
III. Description of the Direct Detection Detector
!V. Detector Characterization
V. Radiation Damage
VI. Discussion 7^C
References
Contents
29.
Friedrich Förster and Reiner Heger!
I. Introduction
II. Tomography and Its Increase in Resolution by Averaging
III. Coherent Averaging of
IV. Applications of Tomogram Averaging
V. Conclusion and Outlook
References
30.
Kristinu
I. Introduction
II. Problem Formulation
III. Challenges in Cellular Tomography Segmentation
IV. Important Considerations for Segmentation Methods
V. How
VI. Segmentation Methods for Cellular Tomography
VII.
VIII.
IX.
References
31.
Maryaim
Heather files, ami
I. Introduction HOtl
II. The OH Centered Database Project
III. Looking Ahead
References
Index
Volumes in Series |
| any_adam_object | 1 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Book |
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| index_date | 2024-07-02T17:13:34Z |
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| owner_facet | DE-355 DE-BY-UBR DE-20 DE-703 |
| physical | XXVII, 850 S., [14] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Cellular electron microscopy ed. by J. Richard McIntosh Amsterdam [u.a.] Elsevier, Acad. Press 2007 XXVII, 850 S., [14] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 79 Celbiologie gtt Elektronenmicroscopie gtt Microscopie - Technique Microscopie électronique Cell Physiology Electron microscopy Microscopy Technique Microscopy, Electron methods Zelle (DE-588)4067537-3 gnd rswk-swf Elektronenmikroskopie (DE-588)4014327-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zelle (DE-588)4067537-3 s Elektronenmikroskopie (DE-588)4014327-2 s b DE-604 McIntosh, J. Richard Sonstige oth Methods in cell biology 79 (DE-604)BV002534878 79 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015597306&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cellular electron microscopy Methods in cell biology Celbiologie gtt Elektronenmicroscopie gtt Microscopie - Technique Microscopie électronique Cell Physiology Electron microscopy Microscopy Technique Microscopy, Electron methods Zelle (DE-588)4067537-3 gnd Elektronenmikroskopie (DE-588)4014327-2 gnd |
| subject_GND | (DE-588)4067537-3 (DE-588)4014327-2 (DE-588)4143413-4 |
| title | Cellular electron microscopy |
| title_auth | Cellular electron microscopy |
| title_exact_search | Cellular electron microscopy |
| title_exact_search_txtP | Cellular electron microscopy |
| title_full | Cellular electron microscopy ed. by J. Richard McIntosh |
| title_fullStr | Cellular electron microscopy ed. by J. Richard McIntosh |
| title_full_unstemmed | Cellular electron microscopy ed. by J. Richard McIntosh |
| title_short | Cellular electron microscopy |
| title_sort | cellular electron microscopy |
| topic | Celbiologie gtt Elektronenmicroscopie gtt Microscopie - Technique Microscopie électronique Cell Physiology Electron microscopy Microscopy Technique Microscopy, Electron methods Zelle (DE-588)4067537-3 gnd Elektronenmikroskopie (DE-588)4014327-2 gnd |
| topic_facet | Celbiologie Elektronenmicroscopie Microscopie - Technique Microscopie électronique Cell Physiology Electron microscopy Microscopy Technique Microscopy, Electron methods Zelle Elektronenmikroskopie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015597306&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT mcintoshjrichard cellularelectronmicroscopy |