Neutrophil methods and protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2007
|
| Series: | Methods in molecular biology
412 |
| Subjects: | |
| Online Access: | Table of contents only Inhaltsverzeichnis Klappentext |
| Item Description: | Auf der Rücks. d. Titelbl. fälschlicherweise: Methods in molecular biology ; 138 |
| Physical Description: | XX, 537 S. Ill., graph. Darst. |
| ISBN: | 9781588297884 1588297888 |
Staff View
MARC
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| 245 | 1 | 0 | |a Neutrophil methods and protocols |c ed. by Mark T. Quinn ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2007 | |
| 300 | |a XX, 537 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 412 | |
| 500 | |a Auf der Rücks. d. Titelbl. fälschlicherweise: Methods in molecular biology ; 138 | ||
| 650 | 4 | |a Neutrophiles - Manuels de laboratoire | |
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| 650 | 4 | |a Neutrophils |v Laboratory manuals | |
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| adam_text | Contents
Dedication
........................................................................................................
v
Preface
...........................................................................................................
vii
Contributors
..................................................................................................xiii
Color Insert
.....................................................................................................xix
Part I. Neutrophils: An Overview
1
The Role of Neutrophils in the Immune System: An Overview
Harry L.
Málech
....................................................................................
З
Part
fl.
Neutrophil Isolation and Subcellular Fractionation
2
Isolation of Human Neutrophils from Venous Blood
William M. Nauseef
............................................................................15
3
Neutrophil Isolation from Non-Human Species
Daniel W. Siemsen, Igor A. Schepetkin,
Lilia
N.
Kirpotina,
Benfang
Lei and Mark T. Quinn
.....................................................21
4
Subcellular Fractionation of Human Neutrophils and Analysis
of Subcellular Markers
Lene
Udby and Niels Borregaard
.......................................................35
Part III. Biochemistry, Biology and Signal Transduction
of Neutrophils
5
Rac
and Rap GTPase Activation Assays
Ulla
G.
Knaus, Alison
Bamberg
and Gary
M. Bokoch
........................59
6
Measurement of Phospholipid Metabolism in Intact Neutrophils
Susan Sergeant and Linda C. McPhail
................................................69
7
Analysis of Protein Phosphorylation in Human Neutrophils
Jamel El-Benna and Pham My-Chan Dang
..........................................85
8
Mitogen-Activated Protein Kinase Assays
Martine
Torres
....................................................................................97
9
Transduction of Proteins into Intact Neutrophils
Tieming Zhao and Gary
M. Bokoch
.....................·............................115
10
Optical Methods for the Measurement and Manipulation
of Cytosolic Free Calcium in Neutrophils
Esther J. Hillson, Sharon Dewitt and Maurice
B. Hallett
.................125
ix
x
Contents
11
Analysis of Electrophysiological Properties and Responses
of Neutrophils
Deri Morgan and Thomas E. DeCoursey
..........................................139
12
Analysis of Neutrophil Apoptosis
Emma
L
Taylor,
Adriano
G.
Rossi, Ian
Dransfield
and
Simon P.
Hart........................................................................ 177
Part
IV.
Neutrophil
Adhesion and Chemotaxis
13
Optical and Fluorescence Detection
of Neutrophil
Integrin
Activation
Ulrich Y.
Schaff,
Melissa
R. Sarantos,
Harold Ting
and Scott I. Simon
........................................................................203
14
Analysis of Neutrophil Polarization and Chemotaxis
Mary A. Lokuta, Paul A. Nuzzi and Anna Huttenlocher
..................211
15
Quantifying and Localizing Actin Free
Barbed Ends in Neutrophils
Michael Glogauer
.............................................................................231
16
Measurement of Neutrophil Adhesion
Under Conditions Mimicking Blood Flow
Mark
A. lutila,
Bruce Walcheck, Robert
Bargatze
and Aiyappa Palecanda
................................................................239
1 7
Model Systems to Investigate Neutrophil
Adhesion and Chemotaxis
Nancy A. Louis, Eric Campbell and Sean Colgan
.............................257
Part V. Neutrophil Phagocytosis and Bactericidal Activity
18
Immunofluorescence and Confocal
Microscopy of Neutrophils
Lee-Ann H. Allen
...............................................................................273
19
Assessment of Phagosome Formation and Maturation
by Fluorescence Microscopy
Benjamin E. Steinberg and Sergio Grinstein
.....................................289
20
Analysis of Neutrophil Membrane Traffic During Phagocytosis
Per
Lönnbro, Pontus
Nordenfeit
and Hans Tapper
..........................301
21
Analysis of Neutrophil Bactericidal Activity
Jessie
N.
Green, Christine
C. Winterbourn
and Mark B. Hampton
..................................................................319
22
A Skin Chamber Technique as a Human Model for Studies
of Aseptic Inflammatory Reactions
Per
Follín
and
Claes Dahlgren..........................................................
333
Contents xi
Part VI. NADPH
Oxidase
and Production
of Reactive Oxygen Species
23
Measurement of Respiratory Bursts Products Generated
by Professional Phagocytes
Claes Dahlgren,
Anna
Karlsson
and
Johan Bylund
...........................349
24
The
К-562
Cell Model for Analysis
of
NeutrophóÔ
NADPH
Oxidase
Function
Thomas
L. Leto,
Mark C. Lavigne, Neda Homoyounpour,
Kristen Lekström,
Gilda
Linton, Harry
L.
Málech
and
Isabelle de
Méndez
................................................................365
25
Cell-free Assays: The Reductionist Approach to the Study
of NADPH
Oxidase
Assembly
Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev,
Iris Dahan, Yevgney Berdichevsky and Edgar Pick
......................385
26
Immunoaffinity Purification of Human Phagocyte
Flavocytochrome
b
and Analysis
of Conformational Dynamics
Ross M. Taylor and Algirdas J. Jesaitis
..............................................429
Part
VII.
Analysis of Neutrophil Gene Expression
and Transcription Factors
27
Genome-scale Transcript Analyses with Human Neutrophils
Scott D. Kobayashi, Dan E. Sturdevant and Frank R. DeLeo
...........441
28
Fast and Accurate Quantitative Analysis
of Cytokine Gene Expression in Human Neutrophils
by Reverse Transcription Real-Time PCR
Nicola Tamassia, Marco A. Cassatella and Flavia
Bazzoni
..............455
29
Detection of Intact Transcription Factors in Human Neutrophils
Patrick P. McDonald and Richard D. Ye
..........................................473
Part
VIII.
Neutrophil Defects and Diagnosis
30
Disorders of Neutrophil Function: An Overview
Mary C. Dinauer
...............................................................................489
31
Diagnostic Assays for Chronic Granulomatous Disease
and Other Neutrophil Disorders
Houda Zghal Elloumi and Steven M. Holland
..................................505
32
Diagnostic Assays for Myeloperoxidase Deficiency
William M.
Nauseei
..........................................................................525
Index
............................................................................................................
53Í
Neutrophils,
the most abundant white cells in humans, serve as the primary
cellular defense against infection. This volume provides a concise set of protocols
for assessing basic neutrophil functions, investigating specialized areas in neutrophil
research, and completing step-by-step diagnostic assays of common neutrophil disor¬
ders. Each of the protocols is written by leading researchers in the field and includes
hints for success, as well as guidance for troubleshooting. Part
1
provides an overview
of neutrophils and their role in host defense and inflammation. Part
2
describes the
methods most commonly used for isolating neutrophils from humans and other animal
species, while examining procedures for subcellular fractionation of human neutro¬
phils. Part
3
details methods for investigating adhesion, chemotaxis, phagocytosis and
bacterial activity. Part
4
presents protocols for the measurement of gene expression
in neutrophils, and Part
5
concludes with assays for diagnosing the most common
neutrophil disorders. Neutrophil Methods and Protocols is a comprehensive source for
detailed explanations and applications of the most modern methodological advances
in neutrophil biology. Both basic scientists and clinicians will find a collection of this
caliber to be an invaluable aid in their work with neutrophils.
|
| adam_txt |
Contents
Dedication
.
v
Preface
.
vii
Contributors
.xiii
Color Insert
.xix
Part I. Neutrophils: An Overview
1
The Role of Neutrophils in the Immune System: An Overview
Harry L.
Málech
.
З
Part
fl.
Neutrophil Isolation and Subcellular Fractionation
2
Isolation of Human Neutrophils from Venous Blood
William M. Nauseef
.15
3
Neutrophil Isolation from Non-Human Species
Daniel W. Siemsen, Igor A. Schepetkin,
Lilia
N.
Kirpotina,
Benfang
Lei and Mark T. Quinn
.21
4
Subcellular Fractionation of Human Neutrophils and Analysis
of Subcellular Markers
Lene
Udby and Niels Borregaard
.35
Part III. Biochemistry, Biology and Signal Transduction
of Neutrophils
5
Rac
and Rap GTPase Activation Assays
Ulla
G.
Knaus, Alison
Bamberg
and Gary
M. Bokoch
.59
6
Measurement of Phospholipid Metabolism in Intact Neutrophils
Susan Sergeant and Linda C. McPhail
.69
7
Analysis of Protein Phosphorylation in Human Neutrophils
Jamel El-Benna and Pham My-Chan Dang
.85
8
Mitogen-Activated Protein Kinase Assays
Martine
Torres
.97
9
Transduction of Proteins into Intact Neutrophils
Tieming Zhao and Gary
M. Bokoch
.·.115
10
Optical Methods for the Measurement and Manipulation
of Cytosolic Free Calcium in Neutrophils
Esther J. Hillson, Sharon Dewitt and Maurice
B. Hallett
.125
ix
x
Contents
11
Analysis of Electrophysiological Properties and Responses
of Neutrophils
Deri Morgan and Thomas E. DeCoursey
.139
12
Analysis of Neutrophil Apoptosis
Emma
L
Taylor,
Adriano
G.
Rossi, Ian
Dransfield
and
Simon P.
Hart. 177
Part
IV.
Neutrophil
Adhesion and Chemotaxis
13
Optical and Fluorescence Detection
of Neutrophil
Integrin
Activation
Ulrich Y.
Schaff,
Melissa
R. Sarantos,
Harold Ting
and Scott I. Simon
.203
14
Analysis of Neutrophil Polarization and Chemotaxis
Mary A. Lokuta, Paul A. Nuzzi and Anna Huttenlocher
.211
15
Quantifying and Localizing Actin Free
Barbed Ends in Neutrophils
Michael Glogauer
.231
16
Measurement of Neutrophil Adhesion
Under Conditions Mimicking Blood Flow
Mark
A. lutila,
Bruce Walcheck, Robert
Bargatze
and Aiyappa Palecanda
.239
1 7
Model Systems to Investigate Neutrophil
Adhesion and Chemotaxis
Nancy A. Louis, Eric Campbell and Sean Colgan
.257
Part V. Neutrophil Phagocytosis and Bactericidal Activity
18
Immunofluorescence and Confocal
Microscopy of Neutrophils
Lee-Ann H. Allen
.273
19
Assessment of Phagosome Formation and Maturation
by Fluorescence Microscopy
Benjamin E. Steinberg and Sergio Grinstein
.289
20
Analysis of Neutrophil Membrane Traffic During Phagocytosis
Per
Lönnbro, Pontus
Nordenfeit
and Hans Tapper
.301
21
Analysis of Neutrophil Bactericidal Activity
Jessie
N.
Green, Christine
C. Winterbourn
and Mark B. Hampton
.319
22
A Skin Chamber Technique as a Human Model for Studies
of Aseptic Inflammatory Reactions
Per
Follín
and
Claes Dahlgren.
333
Contents xi
Part VI. NADPH
Oxidase
and Production
of Reactive Oxygen Species
23
Measurement of Respiratory Bursts Products Generated
by Professional Phagocytes
Claes Dahlgren,
Anna
Karlsson
and
Johan Bylund
.349
24
The
К-562
Cell Model for Analysis
of
NeutrophóÔ
NADPH
Oxidase
Function
Thomas
L. Leto,
Mark C. Lavigne, Neda Homoyounpour,
Kristen Lekström,
Gilda
Linton, Harry
L.
Málech
and
Isabelle de
Méndez
.365
25
Cell-free Assays: The Reductionist Approach to the Study
of NADPH
Oxidase
Assembly
Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev,
Iris Dahan, Yevgney Berdichevsky and Edgar Pick
.385
26
Immunoaffinity Purification of Human Phagocyte
Flavocytochrome
b
and Analysis
of Conformational Dynamics
Ross M. Taylor and Algirdas J. Jesaitis
.429
Part
VII.
Analysis of Neutrophil Gene Expression
and Transcription Factors
27
Genome-scale Transcript Analyses with Human Neutrophils
Scott D. Kobayashi, Dan E. Sturdevant and Frank R. DeLeo
.441
28
Fast and Accurate Quantitative Analysis
of Cytokine Gene Expression in Human Neutrophils
by Reverse Transcription Real-Time PCR
Nicola Tamassia, Marco A. Cassatella and Flavia
Bazzoni
.455
29
Detection of Intact Transcription Factors in Human Neutrophils
Patrick P. McDonald and Richard D. Ye
.473
Part
VIII.
Neutrophil Defects and Diagnosis
30
Disorders of Neutrophil Function: An Overview
Mary C. Dinauer
.489
31
Diagnostic Assays for Chronic Granulomatous Disease
and Other Neutrophil Disorders
Houda Zghal Elloumi and Steven M. Holland
.505
32
Diagnostic Assays for Myeloperoxidase Deficiency
William M.
Nauseei
.525
Index
.
53Í
Neutrophils,
the most abundant white cells in humans, serve as the primary
cellular defense against infection. This volume provides a concise set of protocols
for assessing basic neutrophil functions, investigating specialized areas in neutrophil
research, and completing step-by-step diagnostic assays of common neutrophil disor¬
ders. Each of the protocols is written by leading researchers in the field and includes
hints for success, as well as guidance for troubleshooting. Part
1
provides an overview
of neutrophils and their role in host defense and inflammation. Part
2
describes the
methods most commonly used for isolating neutrophils from humans and other animal
species, while examining procedures for subcellular fractionation of human neutro¬
phils. Part
3
details methods for investigating adhesion, chemotaxis, phagocytosis and
bacterial activity. Part
4
presents protocols for the measurement of gene expression
in neutrophils, and Part
5
concludes with assays for diagnosing the most common
neutrophil disorders. Neutrophil Methods and Protocols is a comprehensive source for
detailed explanations and applications of the most modern methodological advances
in neutrophil biology. Both basic scientists and clinicians will find a collection of this
caliber to be an invaluable aid in their work with neutrophils. |
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| id | DE-604.BV022282178 |
| illustrated | Illustrated |
| index_date | 2024-07-02T16:49:44Z |
| indexdate | 2024-07-09T20:54:04Z |
| institution | BVB |
| isbn | 9781588297884 1588297888 |
| language | English |
| lccn | 2006031694 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015492470 |
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| physical | XX, 537 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Neutrophil methods and protocols ed. by Mark T. Quinn ... Totowa, NJ Humana Press 2007 XX, 537 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 412 Auf der Rücks. d. Titelbl. fälschlicherweise: Methods in molecular biology ; 138 Neutrophiles - Manuels de laboratoire Neutrophiles ram Neutrophils Laboratory manuals Neutrophils Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Neutrophiler Granulozyt (DE-588)4171673-5 gnd rswk-swf Neutrophiler Granulozyt (DE-588)4171673-5 s Molekularbiologie (DE-588)4039983-7 s b DE-604 Quinn, Mark T. Sonstige oth Methods in molecular biology 412 (DE-604)BV035362695 412 http://www.loc.gov/catdir/toc/ecip071/2006031694.html Table of contents only Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492470&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492470&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Neutrophil methods and protocols Methods in molecular biology Neutrophiles - Manuels de laboratoire Neutrophiles ram Neutrophils Laboratory manuals Neutrophils Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Neutrophiler Granulozyt (DE-588)4171673-5 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4171673-5 |
| title | Neutrophil methods and protocols |
| title_auth | Neutrophil methods and protocols |
| title_exact_search | Neutrophil methods and protocols |
| title_exact_search_txtP | Neutrophil methods and protocols |
| title_full | Neutrophil methods and protocols ed. by Mark T. Quinn ... |
| title_fullStr | Neutrophil methods and protocols ed. by Mark T. Quinn ... |
| title_full_unstemmed | Neutrophil methods and protocols ed. by Mark T. Quinn ... |
| title_short | Neutrophil methods and protocols |
| title_sort | neutrophil methods and protocols |
| topic | Neutrophiles - Manuels de laboratoire Neutrophiles ram Neutrophils Laboratory manuals Neutrophils Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Neutrophiler Granulozyt (DE-588)4171673-5 gnd |
| topic_facet | Neutrophiles - Manuels de laboratoire Neutrophiles Neutrophils Laboratory manuals Neutrophils Laboratory Manuals Molekularbiologie Neutrophiler Granulozyt |
| url | http://www.loc.gov/catdir/toc/ecip071/2006031694.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492470&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492470&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT quinnmarkt neutrophilmethodsandprotocols |