Adenovirus methods and protocols: 1 Adenoviruses, ad vectors, quantitation, and animal models
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2007
|
| Edition: | 2. ed. |
| Series: | Methods in molecular medicine
130 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Physical Description: | XIII, 241 S. Ill., graph. Darst. |
| ISBN: | 9781588295989 1588295982 |
Staff View
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface
..............................................................................................................
v
Contents
of the Companion Volume
................................................................
¡x
Contributors
.....................................................................................................xi
1
Manipulation of Early Region
4.............................................................1
iulie
Boyer
and Gary Ketner
2
Isolation, Growth, and Purification of Defective Adenovirus
Deletion Mutants
............................................................................19
Gary Ketner and Julie Boyer
3
Construction of Adenovirus Type
5
Early Region
1
and
4
Virus Mutants
..................................................................................29
Peter Groitl and Thomas Dobner
4
Construction of Mouse Adenovirus Type
1
Mutants
...........................41
Angela
N.
Cauthen, Amanda R. Welton, and
Katherine
R.
Spindler
5
Generation of
Recombinant
Adenovirus Using the Escherichia
coli
BJ5183 Recombination System
.......................................................61
P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins,
and Neeraja Idamakanti
6
Production and Release Testing of Ovine Atadenovirus Vectors
...........69
Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett,
and Ian Shaw
7
Construction of Capsid-Modified
Recombinant
Bovine Adenovirus
ТуреЗ
.............................................................................................91
Alexander
N.
Zakhartchouk, Qiaohua Wu, and Suresh K. Tikoo
8
Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions/Gene
Replacements in the Major Late Transcription Unit
.....................107
Jason Gall, John Schoggins, and Erik Falck-Pedersen
9
Temperature-Sensitive Replication-Competent Adenovirus shRNA
Vectors to Study Cellular Genes in Virus-Induced Apoptosis
.......125
Thirugnana Subramanian and Govindaswamy Chinnadurai
10
Evaluating Apoptosis in Tumor Cells Infected With Adenovirus
Expressing p53 Tumor Suppressor Gene
.......................................135
Elizabeth Perron, M. Behzad
Zafar,
Amanda
Pister,
Zhen-Guo Wang,
Jun Tian,
and Prem Seth
vu
viii Contents
11
Growth and Purification of Enteric Adenovirus Type
40...................145
Vivien
Mautner
12
lmmunocompetent, Semi-Permissive Cotton Rat Tumor Model
for the Evaluation of Oncolytic Adenoviruses
..............................157
Karoly
loth,
¡acquetine
F.
Spencer, and William S. M. Wold
13
Use of the Syrian Hamster as an Animal Model for Oncolytic
Adenovirus Vectors
.......................................................................169
Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold
14
A Real-Time PCR Method to Rapidly
Titer
Adenovirus Stocks
..........185
Maria A. Thomas, Drew L.
Lichtenstein,
Peter Krajcsi,
and William S. M. Wold
15
Detection and Quantitation of Subgroup
С
Adenovirus
DNA
in Human Tissue Samples by Real-Time PCR
...............................193
C. T. Garnett,
Ching-I
Pao,
and Linda R. Gooding
16
Flow Cytometric Detection of Adenoviruses and Intracellular
Adenovirus Proteins
......................................................................205
Graham Bottley, John R. Holt, Nicola J. fames, and G. Eric Blah
17
Capture
ELISA
Quantitation of Mouse Adenovirus Type
1
in Infected Organs
.........................................................................215
Amanda R. Welton and
Katherine R.
Spindler
18
Preparation and
Titration
of CsCl-Banded Adenovirus Stocks
..........223
Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova,
Konstantin Doronin,
and William
S. M.
Wold
Index
............................................................................................................237
Adenoviras Methods and Protocols
Second Edition, Volume
1:
Adenoviruses, Ad Vectors, Quantitation, and Animal Models
Edited by
William S. M. Wold and Ann E. Tollefson
Department of Molecular Microbiology and Immunology,
Saint Louis University School of Medicine, St Louis, Missouri
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an
essential resource for adenovirus (Ad) researchers beginning in the field, and an
inspirational starting point for researchers looking to branch into new areas of
Ad study. In addition to updating and expanding important chapters from the first
edition, the authors have added new chapters that address innovative, exciting areas
of emphasis in Ad research, including Ad vector construction and use, real-time PCR,
use of new animal models, and methods for quantification of Ad virus or virus expres¬
sion/interactions. Each of the protocols presented in these volumes is written by
trendsetting researchers in their respective areas of expertise.
Volume
1
addresses several important techniques for construction of adeno¬
viruses for use as vectors and for basic research. Highlighted topics include deletion
mutants, capsid modifications, insertions, and gene replacements in human,
murine,
bovine, and ovine adenoviruses. In Volume
2,
the authors focus on methods that
elucidate and quantitate the interactions of Ad with the host Each of the protocols
in these volumes provides a general introduction, followed by tried-and-true step-
by-step methods. Both novice and experienced researchers will reap tremendous
benefit from these groundbreaking volumes in Ad research.
Features
•
Detailed description of quality
control testing of vectors
•
Methods for determination
of virus titters
•
Sensitive assays for testing viral
DNA
presence in samples
•
Developing new, permissive
immanocompetent animal models
•
Characterization of specific
Ad proteins (hexon, fiber, or
protease/proteinase)
•
Procedures for microinjectHMi,
transformation assays, and NK
cell-mediated
cytołysis
•
Analysis of phyiogenetics and
genetic variability
|
| adam_txt |
Contents
Preface
.
v
Contents
of the Companion Volume
.
¡x
Contributors
.xi
1
Manipulation of Early Region
4.1
iulie
Boyer
and Gary Ketner
2
Isolation, Growth, and Purification of Defective Adenovirus
Deletion Mutants
.19
Gary Ketner and Julie Boyer
3
Construction of Adenovirus Type
5
Early Region
1
and
4
Virus Mutants
.29
Peter Groitl and Thomas Dobner
4
Construction of Mouse Adenovirus Type
1
Mutants
.41
Angela
N.
Cauthen, Amanda R. Welton, and
Katherine
R.
Spindler
5
Generation of
Recombinant
Adenovirus Using the Escherichia
coli
BJ5183 Recombination System
.61
P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins,
and Neeraja Idamakanti
6
Production and Release Testing of Ovine Atadenovirus Vectors
.69
Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett,
and Ian Shaw
7
Construction of Capsid-Modified
Recombinant
Bovine Adenovirus
ТуреЗ
.91
Alexander
N.
Zakhartchouk, Qiaohua Wu, and Suresh K. Tikoo
8
Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions/Gene
Replacements in the Major Late Transcription Unit
.107
Jason Gall, John Schoggins, and Erik Falck-Pedersen
9
Temperature-Sensitive Replication-Competent Adenovirus shRNA
Vectors to Study Cellular Genes in Virus-Induced Apoptosis
.125
Thirugnana Subramanian and Govindaswamy Chinnadurai
10
Evaluating Apoptosis in Tumor Cells Infected With Adenovirus
Expressing p53 Tumor Suppressor Gene
.135
Elizabeth Perron, M. Behzad
Zafar,
Amanda
Pister,
Zhen-Guo Wang,
Jun Tian,
and Prem Seth
vu
viii Contents
11
Growth and Purification of Enteric Adenovirus Type
40.145
Vivien
Mautner
12
lmmunocompetent, Semi-Permissive Cotton Rat Tumor Model
for the Evaluation of Oncolytic Adenoviruses
.157
Karoly
loth,
¡acquetine
F.
Spencer, and William S. M. Wold
13
Use of the Syrian Hamster as an Animal Model for Oncolytic
Adenovirus Vectors
.169
Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold
14
A Real-Time PCR Method to Rapidly
Titer
Adenovirus Stocks
.185
Maria A. Thomas, Drew L.
Lichtenstein,
Peter Krajcsi,
and William S. M. Wold
15
Detection and Quantitation of Subgroup
С
Adenovirus
DNA
in Human Tissue Samples by Real-Time PCR
.193
C. T. Garnett,
Ching-I
Pao,
and Linda R. Gooding
16
Flow Cytometric Detection of Adenoviruses and Intracellular
Adenovirus Proteins
.205
Graham Bottley, John R. Holt, Nicola J. fames, and G. Eric Blah
17
Capture
ELISA
Quantitation of Mouse Adenovirus Type
1
in Infected Organs
.215
Amanda R. Welton and
Katherine R.
Spindler
18
Preparation and
Titration
of CsCl-Banded Adenovirus Stocks
.223
Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova,
Konstantin Doronin,
and William
S. M.
Wold
Index
.237
Adenoviras Methods and Protocols
Second Edition, Volume
1:
Adenoviruses, Ad Vectors, Quantitation, and Animal Models
Edited by
William S. M. Wold and Ann E. Tollefson
Department of Molecular Microbiology and Immunology,
Saint Louis University School of Medicine, St Louis, Missouri
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an
essential resource for adenovirus (Ad) researchers beginning in the field, and an
inspirational starting point for researchers looking to branch into new areas of
Ad study. In addition to updating and expanding important chapters from the first
edition, the authors have added new chapters that address innovative, exciting areas
of emphasis in Ad research, including Ad vector construction and use, real-time PCR,
use of new animal models, and methods for quantification of Ad virus or virus expres¬
sion/interactions. Each of the protocols presented in these volumes is written by
trendsetting researchers in their respective areas of expertise.
Volume
1
addresses several important techniques for construction of adeno¬
viruses for use as vectors and for basic research. Highlighted topics include deletion
mutants, capsid modifications, insertions, and gene replacements in human,
murine,
bovine, and ovine adenoviruses. In Volume
2,
the authors focus on methods that
elucidate and quantitate the interactions of Ad with the host Each of the protocols
in these volumes provides a general introduction, followed by tried-and-true step-
by-step methods. Both novice and experienced researchers will reap tremendous
benefit from these groundbreaking volumes in Ad research.
Features
•
Detailed description of quality
control testing of vectors
•
Methods for determination
of virus titters
•
Sensitive assays for testing viral
DNA
presence in samples
•
Developing new, permissive
immanocompetent animal models
•
Characterization of specific
Ad proteins (hexon, fiber, or
protease/proteinase)
•
Procedures for microinjectHMi,
transformation assays, and NK
cell-mediated
cytołysis
•
Analysis of phyiogenetics and
genetic variability |
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| id | DE-604.BV022282162 |
| illustrated | Illustrated |
| index_date | 2024-07-02T16:49:43Z |
| indexdate | 2024-07-09T20:54:04Z |
| institution | BVB |
| isbn | 9781588295989 1588295982 |
| language | English |
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| physical | XIII, 241 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Adenovirus methods and protocols 1 Adenoviruses, ad vectors, quantitation, and animal models ed. by William S. M. Wold ... 2. ed. Totowa, NJ Humana Press 2007 XIII, 241 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 130 Methods in molecular medicine ... Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviruses Laboratory manuals Molecular biology Laboratory manuals Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Adenoviren (DE-588)4141412-3 gnd rswk-swf Adenoviren (DE-588)4141412-3 s Molekulare Medizin (DE-588)4543844-4 s DE-604 Wold, William S. M. Sonstige oth (DE-604)BV022282157 1 Methods in molecular medicine 130 (DE-604)BV011334960 130 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492454&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492454&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Adenovirus methods and protocols Methods in molecular medicine Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviruses Laboratory manuals Molecular biology Laboratory manuals Molekulare Medizin (DE-588)4543844-4 gnd Adenoviren (DE-588)4141412-3 gnd |
| subject_GND | (DE-588)4543844-4 (DE-588)4141412-3 |
| title | Adenovirus methods and protocols |
| title_auth | Adenovirus methods and protocols |
| title_exact_search | Adenovirus methods and protocols |
| title_exact_search_txtP | Adenovirus methods and protocols |
| title_full | Adenovirus methods and protocols 1 Adenoviruses, ad vectors, quantitation, and animal models ed. by William S. M. Wold ... |
| title_fullStr | Adenovirus methods and protocols 1 Adenoviruses, ad vectors, quantitation, and animal models ed. by William S. M. Wold ... |
| title_full_unstemmed | Adenovirus methods and protocols 1 Adenoviruses, ad vectors, quantitation, and animal models ed. by William S. M. Wold ... |
| title_short | Adenovirus methods and protocols |
| title_sort | adenovirus methods and protocols adenoviruses ad vectors quantitation and animal models |
| topic | Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviruses Laboratory manuals Molecular biology Laboratory manuals Molekulare Medizin (DE-588)4543844-4 gnd Adenoviren (DE-588)4141412-3 gnd |
| topic_facet | Adenoviridae Adenoviridae laboratory manuals Adenoviruses Laboratory manuals Molecular biology Laboratory manuals Molekulare Medizin Adenoviren |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492454&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492454&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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