Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells:
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| Format: | Microfilm Book |
| Language: | English |
| Published: |
2006
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Kiel, Univ., Diss., 2006 |
| Physical Description: | 78 Bl. graph. Darst. |
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| 245 | 1 | 0 | |a Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |c vorgelegt von Dong-Hui Zhou |
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Record in the Search Index
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| adam_text | Contents
Abbreviations I
1. Introduction 1
1.1 Overview of the major apoptotic pathways 2
1.2 The relationship of TRAIL and apoptosis 3
1.2.1 TRAIL 3
1.2.2 The TRAIL death receptors 3
1.2.3 Molecular mechanisms of TRAIL induced apoptosis 4
1.3 Non apoptotic signaling of death receptors 4
1.3.1 uPA 5
1.3.2 IL 8 6
1.3.3 The family of MMPs 6
1.4 The objective of this study 7
2. Materials and Methods 8
2.1 Cell Lines and cell culture 8
2.1.1 Cell lines 8
2.1.2 Cell culture 9
2.2 Crystal violet cell vitality assay 10
2.3 Cell treatment with TRAIL and / or pharmacological inhibitors 10
2.4. RNA preparation 11
2.4.1 RNA purification 11
2.5 Synthesis of first strand cDNA by reverse transcription 12
2.6 Real Time PCR 13
2.6.1 Standard calibration curves 13
2.6.2 Real time PCR reactions 13
2.6.3 Verification of PCR results 15
3. Results 16
3.1 TRAIL can dose dependently stimulate overexpression of uPA in pancreatic
cancer cell lines with the different features of apoptosis 16
3.2 IL 8 was also dose dependently over expressed in pancreatic cancer cell lines
treated with TRAIL 20
3.3 MMP9 and MMP7 were dose dependently up regulated by TRAIL in pancreatic
cancer cell lines 23
3.4 TRAF2 dramatically contributes to enhancement of expression of uPA and IL 8
in pancreatic cancer cells 28
3.5 Bcl xL promotes TRAIL induced expression of uPA, IL 8, MMP7 and MMP9 in
pancreatic cancer cells 30
3.6 The TRAIL R1 and TRAIL R2 are differently involved in the regulation of uPA
and IL 8 expression in pancreatic cancer cells 31
3.7 Caspases are involved in TRAIL induced uPA , IL 8 , MMP7 , and
MMP9 expression in pancreatic cancer cells 35
3.8 TRAIL induced overexpression of uPA and IL 8 are prevented by the
PKC inhibitor Goe6983 39
3.9 TRAIL induced overexpression of uPA, IL 8, MMP7 and MMP9 are prevented
by inhibition of MEK1/2 3.10 NF kB is a key mediator in non apoptotic signaling pathway induced by
TRAIL 48
4. Discussion 51
4.1 TRAIL can enhance the invasive properties in pancreatic tumor cell lines while
triggering cell apoptosis and TRAF2 is a positive regulator of this
enhancement 51
4.2 The identification of TRAIL induced non apoptotic signaling transduction
pathway in pancreatic cancer cell line 53
4.2.1 TRAIL R1 andTRAIL R2 are involved in this signaling pathway 53.
4.2.2 The role of caspases in this signaling pathway 55
4.2.3 The role ofPKCs in this signaling pathway 56
4.2.4 The role of MEK in this signaling pathway 58
4.2.5 The role of NF «B in non apoptotic signaling pathway induced by TRAIL.. .59
4.2.6 The role of Bcl xLin non apoptotic signaling pathway induced by TRAIL.. .60
5. Summary 61
6. References 63
Acknowledgments 75
Curriculum Vitae 77
|
| adam_txt |
Contents
Abbreviations I
1. Introduction 1
1.1 Overview of the major apoptotic pathways 2
1.2 The relationship of TRAIL and apoptosis 3
1.2.1 TRAIL 3
1.2.2 The TRAIL death receptors 3
1.2.3 Molecular mechanisms of TRAIL induced apoptosis 4
1.3 Non apoptotic signaling of death receptors 4
1.3.1 uPA 5
1.3.2 IL 8 6
1.3.3 The family of MMPs 6
1.4 The objective of this study 7
2. Materials and Methods 8
2.1 Cell Lines and cell culture 8
2.1.1 Cell lines 8
2.1.2 Cell culture 9
2.2 Crystal violet cell vitality assay 10
2.3 Cell treatment with TRAIL and / or pharmacological inhibitors 10
2.4. RNA preparation 11
2.4.1 RNA purification 11
2.5 Synthesis of first strand cDNA by reverse transcription 12
2.6 Real Time PCR 13
2.6.1 Standard calibration curves 13
2.6.2 Real time PCR reactions 13
2.6.3 Verification of PCR results 15
3. Results 16
3.1 TRAIL can dose dependently stimulate overexpression of uPA in pancreatic
cancer cell lines with the different features of apoptosis 16
3.2 IL 8 was also dose dependently over expressed in pancreatic cancer cell lines
treated with TRAIL 20
3.3 MMP9 and MMP7 were dose dependently up regulated by TRAIL in pancreatic
cancer cell lines 23
3.4 TRAF2 dramatically contributes to enhancement of expression of uPA and IL 8
in pancreatic cancer cells 28
3.5 Bcl xL promotes TRAIL induced expression of uPA, IL 8, MMP7 and MMP9 in
pancreatic cancer cells 30
3.6 The TRAIL R1 and TRAIL R2 are differently involved in the regulation of uPA
and IL 8 expression in pancreatic cancer cells 31
3.7 Caspases are involved in TRAIL induced uPA , IL 8 , MMP7 , and
MMP9 expression in pancreatic cancer cells 35
3.8 TRAIL induced overexpression of uPA and IL 8 are prevented by the
PKC inhibitor Goe6983 39
3.9 TRAIL induced overexpression of uPA, IL 8, MMP7 and MMP9 are prevented
by inhibition of MEK1/2 3.10 NF kB is a key mediator in non apoptotic signaling pathway induced by
TRAIL 48
4. Discussion 51
4.1 TRAIL can enhance the invasive properties in pancreatic tumor cell lines while
triggering cell apoptosis and TRAF2 is a positive regulator of this
enhancement 51
4.2 The identification of TRAIL induced non apoptotic signaling transduction
pathway in pancreatic cancer cell line 53
4.2.1 TRAIL R1 andTRAIL R2 are involved in this signaling pathway 53.
4.2.2 The role of caspases in this signaling pathway 55
4.2.3 The role ofPKCs in this signaling pathway 56
4.2.4 The role of MEK in this signaling pathway 58
4.2.5 The role of NF «B in non apoptotic signaling pathway induced by TRAIL. .59
4.2.6 The role of Bcl xLin non apoptotic signaling pathway induced by TRAIL. .60
5. Summary 61
6. References 63
Acknowledgments 75
Curriculum Vitae 77 |
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| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
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| illustrated | Illustrated |
| index_date | 2024-07-02T16:27:42Z |
| indexdate | 2024-07-09T20:52:35Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015428145 |
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| physical | 78 Bl. graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
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| spelling | Zhou, Dong-Hui 1965- Verfasser (DE-588)131627244 aut Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells vorgelegt von Dong-Hui Zhou 2006 78 Bl. graph. Darst. txt rdacontent h rdamedia he rdacarrier Kiel, Univ., Diss., 2006 Mikroform-Ausgabe 2006 1 Mikrofiche : 24x s2006 [Mikrofiche-Ausg.] Bauchspeicheldrüsenkrebs Hochschulschrift Signaltransduktion Tumor-Nekrose-Faktor (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Zhou, Dong-Hui, 1965- Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells 2006 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015428145&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhou, Dong-Hui 1965- Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells Bauchspeicheldrüsenkrebs Hochschulschrift Signaltransduktion Tumor-Nekrose-Faktor |
| subject_GND | (DE-588)4113937-9 |
| title | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |
| title_auth | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |
| title_exact_search | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |
| title_exact_search_txtP | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |
| title_full | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells vorgelegt von Dong-Hui Zhou |
| title_fullStr | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells vorgelegt von Dong-Hui Zhou |
| title_full_unstemmed | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells vorgelegt von Dong-Hui Zhou |
| title_short | Investigation of the non-apoptotic signaling of TRAIL (TNF-related apoptosis-inducing ligand) in human pancreatic tumor cells |
| title_sort | investigation of the non apoptotic signaling of trail tnf related apoptosis inducing ligand in human pancreatic tumor cells |
| topic | Bauchspeicheldrüsenkrebs Hochschulschrift Signaltransduktion Tumor-Nekrose-Faktor |
| topic_facet | Bauchspeicheldrüsenkrebs Hochschulschrift Signaltransduktion Tumor-Nekrose-Faktor |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015428145&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zhoudonghui investigationofthenonapoptoticsignalingoftrailtnfrelatedapoptosisinducingligandinhumanpancreatictumorcells |