Glycobiology protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2006
|
| Series: | Methods in molecular biology
347 |
| Subjects: | |
| Online Access: | Klappentext Inhaltsverzeichnis |
| Physical Description: | XVIII, 381 S. Ill., graph. Darst. |
| ISBN: | 1588295532 |
Staff View
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Record in the Search Index
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| adam_text | Glycobiology Protocols
Edited by
Inka Brockhausen
Departments
Kingston, Ontario, Canada
A distinguished panel of international experts reviews the importance of glycobiology, an interdis¬
ciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology
Protocols highlights important methodological progress that has been made in the field of glycobiology,
helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycocon-
jugates, and on the functions of
Glycobiology Protocols contains specific methods for the analysis of the structures or functions of
glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans.
The methods described for specific systems can be modified for investigations of similar biomolecules and
tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with
a solid basis for studying the many aspects of glycobiology with state-of-the-art methods.
The protocols follow the successful Methods in Molecular Biology™series format, with each one
offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique,
lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
FEATURES
Cutting-edge techniques to study complex
carbohydrates and posttranslational modifications
of proteins
Specific methods for the analysis of the structures
or functions of glycoconjugates
Step-by-step instructions that ensure successful
results
Tricks of the trade and notes on troubleshooting
and avoiding known pitfalls
CONTENTS
Application of Fluorophore-Assisted Carbohydrate Electrophore-
sis for the Study of the Dolichol Pyrophosphate-tinked Oligosac-
charides Pathway in Cell Cultures and Animal Tissues. Partial
Purification of Mannosylphosphorylundecaprenol Synthase
From Micrococcus luteus: A Useful Enzyme for the Biosynthesis
of a Variety ofMannosylphosphorylpolyisoprenol Products. As¬
say of Dolichyl-Phospho-Mannose Synthase Reconstituted in a
Lipid
Analysis of Unusual Forms of OGlycosylation. Structural Anal¬
ysis of Naturally Occurring Sialic Acids. Nonradioactive Trans-
Sialidase Screening Assay. Structural Determination of OGly-
cans by Tandem Mass Spectrometry. Detailed Structural Analysis
of N-Glycans Released From Glycoproteins in SDS-PAGE Gel
Bands Using HPLC Combined With Exoglycosidase Array Diges¬
tions. Molecular Modeling of Glycosyltransferases.
side a2,6-Sialyltransferase and the Sialyl a2,6-Galactosyl-Link-
age in Tissues and Cell Lines. Visualizing Intracellular
Distribution and Activity of Core2
transferase-1 in Living Cells. Gene Expression Analysis of Glyco-
sylation-Related Genes by Real-Time Polymerase Chain Reaction.
Analysis of the Glycodynamics of Primary Osteoblasts and Bone
Cancer Cells. Micromethods for the Characterization of
Methods in Molecular Biology™
GLYCOBIOLOGY PROTOCOLS
ISBN:
ISSN:
and O-Antigen Lipopolysaccharide. Assay for a Galactosyltrans-
ferase Involved in the Assembly of the O7-Antigen Repeat Unit of
Escherìchia coli.
Labeled
Utilizing Binding to Cationic Dyes. Quantitative Analysis of Mucins
in Mucosal Secretions Using Indirect Enzyme-Linked Immunosor-
bent Assay. Molecular Dissection of the Mouse
Electron-Microscopic Perspective Utilizing High-Resolution Colloi-
dal-GoldLabeling Methods. Soluble Adamantyl Glycosphingolipid
Analogs as Probes of Glycosphingolipid Function. Diagnosis of
Krabbe
Functions of Calnexin and Caireticulin,
Endoplasmi Retìculum.
Ligand Interactions: Assays to Identify a Sweet PHI in a Library of
Carbohydrates. Binding and Inhibition Assays for Siglecs. Index.
ISBN
81588 295538
90000
Contents
Preface
Contributors
1.
for the Study of the Dolichol Pyrophosphate-Linked
Oligosaccharides Pathway in Cell Cultures and Animal Tissues
Ningguo
2.
From Micrococcus luteus: A Useful Enzyme for the Biosynthesis
of a Variety of Mannosylphosphorylpolyisoprenol Products
Jeffrey S. Rush and Charles J. Waechter
3.
in
John S.
4.
lamao Endo
5.
Aleksandra
6.
Johannis P.
7.
Silke Schrader
8.
Catherine
9.
From Glycoproteins in SDS-PAGE Gel Bands Using HPLC
Combined With Exoglycosidase Array Digestions
Louise Royle, Catherine M. Radcliffe, Raymond A. Dwek,
and Pauline M. Rudd
10.
Anne
and
11. ß-Galactoside a2,6-Sialyltransferase
oc2/6-Galactosyl-Linkage in
Fabio Dall Olio,
xi
x¡¡
12.
ß(1,6)N-AcetylgIucosaminyltransferase-I ¡n
Maëlle
13.
by Real-Time Polymerase Chain Reaction
Juan J. Garcia-Vallejo,
and
14.
and Bone Cancer Cells
Inka Brockhausen,
15.
and O-Antigen Lipopolysaccharide
Cristina
and Miguel A. Valvano
16.
of the O7-Antigen Repeat Unit of Escherichia
Inka Brockhausen,
and Jason
17.
Anionie Glycoconjugates
Utilizing Binding to Cationic Dyes
Karen J. Rees-Milton and Tassos P. Anastassiades
18.
Indirect Enzyme-Linked Immunosorbent Assay
Pablo Argiieso and
19.
An Electron-Microscopic Perspective Utilizing High-Resolution
Colloidal-Cold Labeling Methods
Frederick W. K. Kan
20.
of Glycosphingolipid Function
Clifford Lingwood, Skanda Sadacharan,
Murugespillai Mylvaganum, and Marcus Peter
21.
John W.
22.
Lectin Chaperones of the Endoplasmic Reticulum
Breanna S. Ireland,
23.
Assays to Identify a Sweet Pill in a Library of Carbohydrates
Mark E. Beauharnois, Sriram Neelamegham, and Khushi L
Contents xiii
24.
Nadine
Index
|
| adam_txt |
Glycobiology Protocols
Edited by
Inka Brockhausen
Departments
Kingston, Ontario, Canada
A distinguished panel of international experts reviews the importance of glycobiology, an interdis¬
ciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology
Protocols highlights important methodological progress that has been made in the field of glycobiology,
helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycocon-
jugates, and on the functions of
Glycobiology Protocols contains specific methods for the analysis of the structures or functions of
glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans.
The methods described for specific systems can be modified for investigations of similar biomolecules and
tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with
a solid basis for studying the many aspects of glycobiology with state-of-the-art methods.
The protocols follow the successful Methods in Molecular Biology™series format, with each one
offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique,
lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
FEATURES
Cutting-edge techniques to study complex
carbohydrates and posttranslational modifications
of proteins
Specific methods for the analysis of the structures
or functions of glycoconjugates
Step-by-step instructions that ensure successful
results
Tricks of the trade and notes on troubleshooting
and avoiding known pitfalls
CONTENTS
Application of Fluorophore-Assisted Carbohydrate Electrophore-
sis for the Study of the Dolichol Pyrophosphate-tinked Oligosac-
charides Pathway in Cell Cultures and Animal Tissues. Partial
Purification of Mannosylphosphorylundecaprenol Synthase
From Micrococcus luteus: A Useful Enzyme for the Biosynthesis
of a Variety ofMannosylphosphorylpolyisoprenol Products. As¬
say of Dolichyl-Phospho-Mannose Synthase Reconstituted in a
Lipid
Analysis of Unusual Forms of OGlycosylation. Structural Anal¬
ysis of Naturally Occurring Sialic Acids. Nonradioactive Trans-
Sialidase Screening Assay. Structural Determination of OGly-
cans by Tandem Mass Spectrometry. Detailed Structural Analysis
of N-Glycans Released From Glycoproteins in SDS-PAGE Gel
Bands Using HPLC Combined With Exoglycosidase Array Diges¬
tions. Molecular Modeling of Glycosyltransferases.
side a2,6-Sialyltransferase and the Sialyl a2,6-Galactosyl-Link-
age in Tissues and Cell Lines. Visualizing Intracellular
Distribution and Activity of Core2
transferase-1 in Living Cells. Gene Expression Analysis of Glyco-
sylation-Related Genes by Real-Time Polymerase Chain Reaction.
Analysis of the Glycodynamics of Primary Osteoblasts and Bone
Cancer Cells. Micromethods for the Characterization of
Methods in Molecular Biology™
GLYCOBIOLOGY PROTOCOLS
ISBN:
ISSN:
and O-Antigen Lipopolysaccharide. Assay for a Galactosyltrans-
ferase Involved in the Assembly of the O7-Antigen Repeat Unit of
Escherìchia coli.
Labeled
Utilizing Binding to Cationic Dyes. Quantitative Analysis of Mucins
in Mucosal Secretions Using Indirect Enzyme-Linked Immunosor-
bent Assay. Molecular Dissection of the Mouse
Electron-Microscopic Perspective Utilizing High-Resolution Colloi-
dal-GoldLabeling Methods. Soluble Adamantyl Glycosphingolipid
Analogs as Probes of Glycosphingolipid Function. Diagnosis of
Krabbe
Functions of Calnexin and Caireticulin,
Endoplasmi Retìculum.
Ligand Interactions: Assays to Identify a Sweet PHI in a Library of
Carbohydrates. Binding and Inhibition Assays for Siglecs. Index.
ISBN
81588 295538
90000
Contents
Preface
Contributors
1.
for the Study of the Dolichol Pyrophosphate-Linked
Oligosaccharides Pathway in Cell Cultures and Animal Tissues
Ningguo
2.
From Micrococcus luteus: A Useful Enzyme for the Biosynthesis
of a Variety of Mannosylphosphorylpolyisoprenol Products
Jeffrey S. Rush and Charles J. Waechter
3.
in
John S.
4.
lamao Endo
5.
Aleksandra
6.
Johannis P.
7.
Silke Schrader
8.
Catherine
9.
From Glycoproteins in SDS-PAGE Gel Bands Using HPLC
Combined With Exoglycosidase Array Digestions
Louise Royle, Catherine M. Radcliffe, Raymond A. Dwek,
and Pauline M. Rudd
10.
Anne
and
11. ß-Galactoside a2,6-Sialyltransferase
oc2/6-Galactosyl-Linkage in
Fabio Dall'Olio,
xi
x¡¡
12.
ß(1,6)N-AcetylgIucosaminyltransferase-I ¡n
Maëlle
13.
by Real-Time Polymerase Chain Reaction
Juan J. Garcia-Vallejo,
and
14.
and Bone Cancer Cells
Inka Brockhausen,
15.
and O-Antigen Lipopolysaccharide
Cristina
and Miguel A. Valvano
16.
of the O7-Antigen Repeat Unit of Escherichia
Inka Brockhausen,
and Jason
17.
Anionie Glycoconjugates
Utilizing Binding to Cationic Dyes
Karen J. Rees-Milton and Tassos P. Anastassiades
18.
Indirect Enzyme-Linked Immunosorbent Assay
Pablo Argiieso and
19.
An Electron-Microscopic Perspective Utilizing High-Resolution
Colloidal-Cold Labeling Methods
Frederick W. K. Kan
20.
of Glycosphingolipid Function
Clifford Lingwood, Skanda Sadacharan,
Murugespillai Mylvaganum, and Marcus Peter
21.
John W.
22.
Lectin Chaperones of the Endoplasmic Reticulum
Breanna S. Ireland,
23.
Assays to Identify a Sweet Pill in a Library of Carbohydrates
Mark E. Beauharnois, Sriram Neelamegham, and Khushi L
Contents xiii
24.
Nadine
Index |
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| illustrated | Illustrated |
| index_date | 2024-07-02T15:46:45Z |
| indexdate | 2024-07-09T20:44:51Z |
| institution | BVB |
| isbn | 1588295532 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015010096 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Glycobiology protocols ed. by Inka Brockhausen Totowa, NJ Humana Press 2006 XVIII, 381 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 347 Glycoconjugates Glycoconjugates Physiological effect Glycoproteins physiology Laboratory Manuals Molecular Biology methods Laboratory Manuals Glykosylierung (DE-588)4269099-7 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Glykoproteine (DE-588)4071905-4 gnd rswk-swf Glykoproteine (DE-588)4071905-4 s Labortechnik (DE-588)4123602-6 s DE-604 Proteine (DE-588)4076388-2 s Glykosylierung (DE-588)4269099-7 s b DE-604 Brockhausen, Inka 1944- Sonstige (DE-588)173090052 oth Methods in molecular biology 347 (DE-604)BV035362695 347 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015010096&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015010096&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Glycobiology protocols Methods in molecular biology Glycoconjugates Glycoconjugates Physiological effect Glycoproteins physiology Laboratory Manuals Molecular Biology methods Laboratory Manuals Glykosylierung (DE-588)4269099-7 gnd Labortechnik (DE-588)4123602-6 gnd Proteine (DE-588)4076388-2 gnd Glykoproteine (DE-588)4071905-4 gnd |
| subject_GND | (DE-588)4269099-7 (DE-588)4123602-6 (DE-588)4076388-2 (DE-588)4071905-4 |
| title | Glycobiology protocols |
| title_auth | Glycobiology protocols |
| title_exact_search | Glycobiology protocols |
| title_exact_search_txtP | Glycobiology protocols |
| title_full | Glycobiology protocols ed. by Inka Brockhausen |
| title_fullStr | Glycobiology protocols ed. by Inka Brockhausen |
| title_full_unstemmed | Glycobiology protocols ed. by Inka Brockhausen |
| title_short | Glycobiology protocols |
| title_sort | glycobiology protocols |
| topic | Glycoconjugates Glycoconjugates Physiological effect Glycoproteins physiology Laboratory Manuals Molecular Biology methods Laboratory Manuals Glykosylierung (DE-588)4269099-7 gnd Labortechnik (DE-588)4123602-6 gnd Proteine (DE-588)4076388-2 gnd Glykoproteine (DE-588)4071905-4 gnd |
| topic_facet | Glycoconjugates Glycoconjugates Physiological effect Glycoproteins physiology Laboratory Manuals Molecular Biology methods Laboratory Manuals Glykosylierung Labortechnik Proteine Glykoproteine |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015010096&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015010096&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT brockhauseninka glycobiologyprotocols |