C. elegans: methods and applications
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2006
|
| Series: | Methods in molecular biology
351 |
| Subjects: | |
| Online Access: | Table of contents only Inhaltsverzeichnis Klappentext |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XII, 292 S. Ill., graph. Darst. |
| ISBN: | 1588295974 9781588295972 |
Staff View
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| 245 | 1 | 0 | |a C. elegans |b methods and applications |c ed. by Kevin Strange |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a XII, 292 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 351 | |
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| adam_text | C. ELEGANS / STRANGE, KEVIN : C2006
TABLE OF CONTENTS / INHALTSVERZEICHNIS
AN OVERVIEW OF C. ELEGANS BIOLOGY / KEVIN STRANGE
COMPARATIVE GENOMICS IN C. ELEGANS, C. BRIGGSAE, AND OTHER CAENORHABDITIS SPECIES / AVRIL COGHLAN, JASON E. STAJICH, AND TODD W. HARRIS
WORMBASE / TODD W. HARRIS AND LINCOLN D. STEIN
C. ELEGANS DELETION MUTANT SCREENING / ROBERT J. BARSTEAD AND DONALD G. MOERMAN
INSERTIONAL MUTAGENESIS IN C. ELEGANS USING THE DROSOPHILA TRANSPOSON MOS1 / JEAN-LOUIS BESSEREAU
SINGLE NUCLEOTIDE POLYMORPHISM MAPPING / M. WAYNE DAVIS AND MARC HAMMARLUND
CREATION OF TRANSGENIC LINES USING MICROPARTICLE BOMBARDMENT METHODS / VIDA PRAITIS
CONSTRUCTION OF PLASMIDS FOR RNA INTERFERENCE AND IN VITRO TRANSCRIPTION OF DOUBLE-STRANDED RNA / LISA TIMMONS
DELIVERY METHODS FOR RNA INTERFERENCE IN C. ELEGANS / LISA TIMMONS
FUNCTIONAL GENOMIC APPROACHES IN C. ELEGANS / TODD LAMITINA
ASSAYS FOR TOXICITY STUDIES IN C. ELEGANS WITH BT CRYSTAL PROTEINS / LARRY J. BISCHOF, DANIELLE L. HUFFMAN, AND RAFFI V. AROIAN
FLUORESCENT REPORTER METHODS / HARALD HUTTER
ELECTROPHYSIOLOGICAL ANALYSIS OF NEURONAL AND MUSCLE FUNCTION IN C. ELEGANS / MICHAEL M. FRANCIS AND ANDRES VILLU MARICQ
SPERM AND OOCYTE ISOLATION METHODS FOR BIOCHEMICAL AND PROTEOMIC ANALYSIS / MICHAEL A. MILLER
PRESERVATION OF C. ELEGANS TISSUE VIA HIGH-PRESSURE FREEZING AND FREEZE-SUBSTITUTION FOR ULTRASTRUCTURAL ANALYSIS AND IMMUNO-CYTOCHEMISTRY / ROBBY M. WEIMER
INTRACELLULAR PH MEASUREMENTS IN VIVO USING GREEN FLUORESCENT PROTEIN VARIANTS / KEITH NEHRKE
AUTOMATED IMAGING OF C. ELEGANS BEHAVIOR / CHRISTOPHER J. CRONIN, ZHAOYANG FENG, AND WILLIAM R. SCHAFER
INTRACELLULAR CA2+ IMAGING IN C. ELEGANS / REX A. KERR AND WILLIAM R. SCHAFER
IN VITRO CULTURE OF C. ELEGANS SOMATIC CELLS / KEVIN STRANGE AND REBECCA MORRISON
TECHNIQUES FOR ANALYSIS, SORTING, AND DISPENSING OF C. ELEGANS ON THE COPAS FLOW-SORTING SYSTEM / ROCK PULAK.
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
С.
Methods and Applications
Edited by
Kevin Strange
Departments of Anesthesiology, Molecular Physiology and Biophysics, and Pharmacology,
Vanderbilt University Medical Center, Nashville, TN
Nonmarnmalian model organisms have become a cornerstone of systems biology research. Like the
Rosetta
enable biologists to decipher the genetic code underlying the complex physiological processes common
toall life.
Genetic, molecular and systems biological characterization of this organism is greatly facilitated by its short
life cycle and high rate reproduction, and by the ease with which it can be cultured in the laboratory.
C. e/egansalso has a fully sequenced and well-annotated genome, which is assembled in readily accessible
public databases along with virtually all other biological data on this organism.
С
study of
art methods currently being utilized in the field. Some of the methods presented include forward and reverse
genetic analysis, data mining and comparative genomics strategies, electron and fluorescence microscopy
methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and
cutting-edge methods for physiological analyses. This comprehensive collection of methods, written by
experts in the field, endeavors to serve as a roadmap for researchers, illustrating the type or research that
is possible with
•
•
imaging
•
at the single worm level and at the population level
Explores methods for physiological analyses,
including somatic cell culture,
eiectrophysiology and in vivo imaging of intracellu-
larCa2łandpH
An Overview of
elegans, C. briggsae, and Other Caenoriabditis Species. Worm-
Base: Methods for Data Mining and Comparative Genomics.
elegans Deletion Mutant Screening. Insertional Mutagenesis in
elegans Using the Drosophik Transposon
Rapid Identification of Mutated Genes. Single-Nucleotide Poly¬
morphism Mapping. Creation
particle Bombardment Methods. Construction of Plasmids for
RNA
RNA.
tional Genomic Approaches in
Studies in
Methods. Electrophysiological Analysis of
Function in
Biochemical and Proteomic Analysis. Preservation of
Tissue Via High-Pressure Freezing and Freeze-Substitution for
Ultrastructural
pH
ants. Automated Imaging of
Ca2ł
Cells. Techniques for Analysis, Sorting, and Dispensing of
С
|
| adam_txt |
C. ELEGANS / STRANGE, KEVIN : C2006
TABLE OF CONTENTS / INHALTSVERZEICHNIS
AN OVERVIEW OF C. ELEGANS BIOLOGY / KEVIN STRANGE
COMPARATIVE GENOMICS IN C. ELEGANS, C. BRIGGSAE, AND OTHER CAENORHABDITIS SPECIES / AVRIL COGHLAN, JASON E. STAJICH, AND TODD W. HARRIS
WORMBASE / TODD W. HARRIS AND LINCOLN D. STEIN
C. ELEGANS DELETION MUTANT SCREENING / ROBERT J. BARSTEAD AND DONALD G. MOERMAN
INSERTIONAL MUTAGENESIS IN C. ELEGANS USING THE DROSOPHILA TRANSPOSON MOS1 / JEAN-LOUIS BESSEREAU
SINGLE NUCLEOTIDE POLYMORPHISM MAPPING / M. WAYNE DAVIS AND MARC HAMMARLUND
CREATION OF TRANSGENIC LINES USING MICROPARTICLE BOMBARDMENT METHODS / VIDA PRAITIS
CONSTRUCTION OF PLASMIDS FOR RNA INTERFERENCE AND IN VITRO TRANSCRIPTION OF DOUBLE-STRANDED RNA / LISA TIMMONS
DELIVERY METHODS FOR RNA INTERFERENCE IN C. ELEGANS / LISA TIMMONS
FUNCTIONAL GENOMIC APPROACHES IN C. ELEGANS / TODD LAMITINA
ASSAYS FOR TOXICITY STUDIES IN C. ELEGANS WITH BT CRYSTAL PROTEINS / LARRY J. BISCHOF, DANIELLE L. HUFFMAN, AND RAFFI V. AROIAN
FLUORESCENT REPORTER METHODS / HARALD HUTTER
ELECTROPHYSIOLOGICAL ANALYSIS OF NEURONAL AND MUSCLE FUNCTION IN C. ELEGANS / MICHAEL M. FRANCIS AND ANDRES VILLU MARICQ
SPERM AND OOCYTE ISOLATION METHODS FOR BIOCHEMICAL AND PROTEOMIC ANALYSIS / MICHAEL A. MILLER
PRESERVATION OF C. ELEGANS TISSUE VIA HIGH-PRESSURE FREEZING AND FREEZE-SUBSTITUTION FOR ULTRASTRUCTURAL ANALYSIS AND IMMUNO-CYTOCHEMISTRY / ROBBY M. WEIMER
INTRACELLULAR PH MEASUREMENTS IN VIVO USING GREEN FLUORESCENT PROTEIN VARIANTS / KEITH NEHRKE
AUTOMATED IMAGING OF C. ELEGANS BEHAVIOR / CHRISTOPHER J. CRONIN, ZHAOYANG FENG, AND WILLIAM R. SCHAFER
INTRACELLULAR CA2+ IMAGING IN C. ELEGANS / REX A. KERR AND WILLIAM R. SCHAFER
IN VITRO CULTURE OF C. ELEGANS SOMATIC CELLS / KEVIN STRANGE AND REBECCA MORRISON
TECHNIQUES FOR ANALYSIS, SORTING, AND DISPENSING OF C. ELEGANS ON THE COPAS FLOW-SORTING SYSTEM / ROCK PULAK.
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
С.
Methods and Applications
Edited by
Kevin Strange
Departments of Anesthesiology, Molecular Physiology and Biophysics, and Pharmacology,
Vanderbilt University Medical Center, Nashville, TN
Nonmarnmalian model organisms have become a cornerstone of systems biology research. Like the
Rosetta
enable biologists to decipher the genetic code underlying the complex physiological processes common
toall life.
Genetic, molecular and systems biological characterization of this organism is greatly facilitated by its short
life cycle and high rate reproduction, and by the ease with which it can be cultured in the laboratory.
C. e/egansalso has a fully sequenced and well-annotated genome, which is assembled in readily accessible
public databases along with virtually all other biological data on this organism.
С
study of
art methods currently being utilized in the field. Some of the methods presented include forward and reverse
genetic analysis, data mining and comparative genomics strategies, electron and fluorescence microscopy
methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and
cutting-edge methods for physiological analyses. This comprehensive collection of methods, written by
experts in the field, endeavors to serve as a roadmap for researchers, illustrating the type or research that
is possible with
•
•
imaging
•
at the single worm level and at the population level
Explores methods for physiological analyses,
including somatic cell culture,
eiectrophysiology and in vivo imaging of intracellu-
larCa2łandpH
An Overview of
elegans, C. briggsae, and Other Caenoriabditis Species. Worm-
Base: Methods for Data Mining and Comparative Genomics.
elegans Deletion Mutant Screening. Insertional Mutagenesis in
elegans Using the Drosophik Transposon
Rapid Identification of Mutated Genes. Single-Nucleotide Poly¬
morphism Mapping. Creation
particle Bombardment Methods. Construction of Plasmids for
RNA
RNA.
tional Genomic Approaches in
Studies in
Methods. Electrophysiological Analysis of
Function in
Biochemical and Proteomic Analysis. Preservation of
Tissue Via High-Pressure Freezing and Freeze-Substitution for
Ultrastructural
pH
ants. Automated Imaging of
Ca2ł
Cells. Techniques for Analysis, Sorting, and Dispensing of
С |
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| id | DE-604.BV021766099 |
| illustrated | Illustrated |
| index_date | 2024-07-02T15:36:48Z |
| indexdate | 2024-07-09T20:43:34Z |
| institution | BVB |
| isbn | 1588295974 9781588295972 |
| language | English |
| lccn | 2006000939 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014979091 |
| oclc_num | 63114161 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-12 DE-20 DE-M49 DE-BY-TUM DE-11 DE-578 DE-19 DE-BY-UBM |
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| physical | XII, 292 S. Ill., graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | C. elegans methods and applications ed. by Kevin Strange Totowa, NJ Humana Press 2006 XII, 292 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 351 Includes bibliographical references and index Caenorhabditis elegans Caenorhabditis elegans Genetics Molecular biology Caenorhabditis elegans physiology Caenorhabditis elegans ultrastructure Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Caenorhabditis elegans (DE-588)4147127-1 gnd rswk-swf Physiologie (DE-588)4045981-0 gnd rswk-swf Caenorhabditis elegans (DE-588)4147127-1 s Physiologie (DE-588)4045981-0 s Labortechnik (DE-588)4123602-6 s DE-604 Molekulargenetik (DE-588)4039987-4 s b DE-604 Strange, Kevin Sonstige oth Methods in molecular biology 351 (DE-604)BV035362695 351 http://www.loc.gov/catdir/toc/ecip066/2006000939.html Table of contents only LoC Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014979091&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014979091&sequence=000006&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | C. elegans methods and applications Methods in molecular biology Caenorhabditis elegans Caenorhabditis elegans Genetics Molecular biology Caenorhabditis elegans physiology Caenorhabditis elegans ultrastructure Molekulargenetik (DE-588)4039987-4 gnd Labortechnik (DE-588)4123602-6 gnd Caenorhabditis elegans (DE-588)4147127-1 gnd Physiologie (DE-588)4045981-0 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4123602-6 (DE-588)4147127-1 (DE-588)4045981-0 |
| title | C. elegans methods and applications |
| title_auth | C. elegans methods and applications |
| title_exact_search | C. elegans methods and applications |
| title_exact_search_txtP | C. elegans methods and applications |
| title_full | C. elegans methods and applications ed. by Kevin Strange |
| title_fullStr | C. elegans methods and applications ed. by Kevin Strange |
| title_full_unstemmed | C. elegans methods and applications ed. by Kevin Strange |
| title_short | C. elegans |
| title_sort | c elegans methods and applications |
| title_sub | methods and applications |
| topic | Caenorhabditis elegans Caenorhabditis elegans Genetics Molecular biology Caenorhabditis elegans physiology Caenorhabditis elegans ultrastructure Molekulargenetik (DE-588)4039987-4 gnd Labortechnik (DE-588)4123602-6 gnd Caenorhabditis elegans (DE-588)4147127-1 gnd Physiologie (DE-588)4045981-0 gnd |
| topic_facet | Caenorhabditis elegans Caenorhabditis elegans Genetics Molecular biology Caenorhabditis elegans physiology Caenorhabditis elegans ultrastructure Molekulargenetik Labortechnik Physiologie |
| url | http://www.loc.gov/catdir/toc/ecip066/2006000939.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014979091&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014979091&sequence=000006&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT strangekevin celegansmethodsandapplications |