Embryonic stem cells: a practical approach
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford [u.a.]
Oxford Univ. Press
2006
|
| Edition: | 1. publ. |
| Series: | Practical approach series
[270] |
| Subjects: | |
| Online Access: | Table of contents only Klappentext Inhaltsverzeichnis |
| Physical Description: | XXV, 334 S. Ill., graph. Darst. |
| ISBN: | 0198550014 9780198550013 0198550006 9780198550006 |
Staff View
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| 245 | 1 | 0 | |a Embryonic stem cells |b a practical approach |c ed. by Elena Notarianni ... |
| 250 | |a 1. publ. | ||
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Record in the Search Index
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| adam_text | The groundbreaking isolation of embryonic stem cells (or
the early
tion. This led to the routine genetic engineering of the mouse and revolutionised our
understanding of biological processes in the context of the whole animal.
ogy remains a crucial and growing area of research with far-reaching implications for
developmental and comparative biology as well as for human health.
This book serves as a primer to
lation. It contains a broad compendium of methods of direct relevance to both gradu¬
ate students and specialist researchers. An introductory chapter by the principle
originator of ES-cell research outlines the fundamentals and charts the development
of the field. This is followed by comprehensive coverage of state-of-the art techniques
for ES-cell manipulation, with the mouse as the experimental paradigm, and by
recent innovations with
therapies for otherwise intractable diseases are now being developed with the pres¬
ent challenge to control
cell transplantation
Approach Series, the emphasis is on current methods from recognised experts.
Cover Colonies of
©
Contents
Protocol
Abbreviations
Contributors
Introduction
Martin]. Evans
1
2
3
Procedures for deriving
Frances A Brook
1
2
3
3.1
3.2
3.3
3.4
3.5
4
4.1
4.2
5
5.1
5.2
6
Production of
Ydko
1
2
vu
CONTENTS
3
3.1
3.2
3.3
with mES cells
4
5
5.1
5.2
5.3
5.4
6
in chimeras
7
in mES cell-derived mice
Generating animal models of human mitochondrial
genetic disease using mouse
Grant R. MacGregor, Wei Wei Fan, Katrina G. Waymire,
and Douglas
1
1.1
1.2
1.3 Generation,
mutations from mice
2
mouse germline
2.1
2.2
mtDNA transfer
2.3
mtDNA polymorphism
2.4
mtDNA polymorphisms
2.5
or synaptosomes with p° or mES cells
2.6
2.7
with R6G
2.8
2.9
of cybrids
2.10
blastocysts for
mES cells
2.11
3
4
vni
CONTENTS
Controlling
cells in vitro
Michael V. Wiles and
1
2
3
3.1
differentiation
3.2
experimental design
3.3
3.4
3.5
4
5
6
In vitro differentiation of mouse
muscle cells
Yelena S. Tarasova, Daniel R. Riordm,
and Kenneth R. Boheler
1
2
muscle cells
3
lineages
3.1
muscle cells
3.2
3.3
4
derivatives
4.1
muscle-cell derivatives
5
differentiation of mES cells
5.1
5.2
reverse-transcription PCR
5.3
6
proteins
6.1
6.2
6.3
cardiac cells
ix
CONTENTS
In vitro
haematopoietic cells
Osamu
1
1.1
1.2
1.3
2
2.1
2.2
2.3
for coculture
2.4
3
differentiation
3.1
3.2
3.3
4
using the mES/OPQ-cell coculture system
5
model for haematopoietic differentiation
lineage selection and transplantation of mouse
ES
Tanja
1
1.1
2
neural precursors
3
differentiated mES cells
3.1
for
3.2 Neuronal
3.3
4
integration of mES cell-derived neural precursors
4.1
cultures
4.2
progenitors
4.3
donor-specific markers
4.4
donor-specific antibodies
5
CONTENTS
In vitro
pancreatic and hepatic cells
Przemysław
1
2
3
3.1
progenitor cells
3.2
4
and hepatic cell phenotypes
4.1
4.2
4.3
4.4
by immunofluorescence
5
5.1
5.2
Isolation and characterization of human
Martin F.
Karen
1
2
2.1
2.2
3
versus serum-free media
4
5
5.1
5.2
5.3
5.4
5.5
Differentiation of human
Sharon
1
2
2.1
3
CONTENTS
4 In vitro
4.1
embryoid bodies
4.2
4.3
5
5.1
5.2
development
6
6.1
6.2
three-dimentional matrices
7
ES
(Macaca fascicularis)
Hirofumi Suemori,
Norio
1
1.1
2
2.1
2.2
2.3
cES cells
3
3.1
4
5
6
6.1
with PA6 cells
6.2
coculture with OP9 cells
7
List of Suppliers
Index
xu
|
| adam_txt |
The groundbreaking isolation of embryonic stem cells (or
the early
tion. This led to the routine genetic engineering of the mouse and revolutionised our
understanding of biological processes in the context of the whole animal.
ogy remains a crucial and growing area of research with far-reaching implications for
developmental and comparative biology as well as for human health.
This book serves as a primer to
lation. It contains a broad compendium of methods of direct relevance to both gradu¬
ate students and specialist researchers. An introductory chapter by the principle
originator of ES-cell research outlines the fundamentals and charts the development
of the field. This is followed by comprehensive coverage of state-of-the art techniques
for ES-cell manipulation, with the mouse as the experimental paradigm, and by
recent innovations with
therapies for otherwise intractable diseases are now being developed with the pres¬
ent challenge to control
cell transplantation
Approach Series, the emphasis is on current methods from recognised experts.
Cover Colonies of
©
Contents
Protocol
Abbreviations
Contributors
Introduction
Martin]. Evans
1
2
3
Procedures for deriving
Frances A Brook
1
2
3
3.1
3.2
3.3
3.4
3.5
4
4.1
4.2
5
5.1
5.2
6
Production of
Ydko
1
2
vu
CONTENTS
3
3.1
3.2
3.3
with mES cells
4
5
5.1
5.2
5.3
5.4
6
in chimeras
7
in mES cell-derived mice
Generating animal models of human mitochondrial
genetic disease using mouse
Grant R. MacGregor, Wei Wei Fan, Katrina G. Waymire,
and Douglas
1
1.1
1.2
1.3 Generation,
mutations from mice
2
mouse germline
2.1
2.2
mtDNA transfer
2.3
mtDNA polymorphism
2.4
mtDNA polymorphisms
2.5
or synaptosomes with p° or mES cells
2.6
2.7
with R6G
2.8
2.9
of cybrids
2.10
blastocysts for
mES cells
2.11
3
4
vni
CONTENTS
Controlling
cells in vitro
Michael V. Wiles and
1
2
3
3.1
differentiation
3.2
experimental design
3.3
3.4
3.5
4
5
6
In vitro differentiation of mouse
muscle cells
Yelena S. Tarasova, Daniel R. Riordm,
and Kenneth R. Boheler
1
2
muscle cells
3
lineages
3.1
muscle cells
3.2
3.3
4
derivatives
4.1
muscle-cell derivatives
5
differentiation of mES cells
5.1
5.2
reverse-transcription PCR
5.3
6
proteins
6.1
6.2
6.3
cardiac cells
ix
CONTENTS
In vitro
haematopoietic cells
Osamu
1
1.1
1.2
1.3
2
2.1
2.2
2.3
for coculture
2.4
3
differentiation
3.1
3.2
3.3
4
using the mES/OPQ-cell coculture system
5
model for haematopoietic differentiation
lineage selection and transplantation of mouse
ES
Tanja
1
1.1
2
neural precursors
3
differentiated mES cells
3.1
for
3.2 Neuronal
3.3
4
integration of mES cell-derived neural precursors
4.1
cultures
4.2
progenitors
4.3
donor-specific markers
4.4
donor-specific antibodies
5
CONTENTS
In vitro
pancreatic and hepatic cells
Przemysław
1
2
3
3.1
progenitor cells
3.2
4
and hepatic cell phenotypes
4.1
4.2
4.3
4.4
by immunofluorescence
5
5.1
5.2
Isolation and characterization of human
Martin F.
Karen
1
2
2.1
2.2
3
versus serum-free media
4
5
5.1
5.2
5.3
5.4
5.5
Differentiation of human
Sharon
1
2
2.1
3
CONTENTS
4 In vitro
4.1
embryoid bodies
4.2
4.3
5
5.1
5.2
development
6
6.1
6.2
three-dimentional matrices
7
ES
(Macaca fascicularis)
Hirofumi Suemori,
Norio
1
1.1
2
2.1
2.2
2.3
cES cells
3
3.1
4
5
6
6.1
with PA6 cells
6.2
coculture with OP9 cells
7
List of Suppliers
Index
xu |
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| id | DE-604.BV021748492 |
| illustrated | Illustrated |
| index_date | 2024-07-02T15:31:30Z |
| indexdate | 2024-07-09T20:43:09Z |
| institution | BVB |
| isbn | 0198550014 9780198550013 0198550006 9780198550006 |
| language | English |
| lccn | 2006000423 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014961733 |
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| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | XXV, 334 S. Ill., graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Oxford Univ. Press |
| record_format | marc |
| series | Practical approach series |
| series2 | Practical approach series |
| spelling | Embryonic stem cells a practical approach ed. by Elena Notarianni ... 1. publ. Oxford [u.a.] Oxford Univ. Press 2006 XXV, 334 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Practical approach series [270] Embryonic stem cells Stem Cells physiology Embryo physiology Mice Models, Animal Embryonale Stammzelle (DE-588)4699648-5 gnd rswk-swf Embryonale Stammzelle (DE-588)4699648-5 s b DE-604 Notarianni, Elena Sonstige oth Practical approach series [270] (DE-604)BV007921321 270 http://www.loc.gov/catdir/toc/ecip066/2006000423.html Table of contents only Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014961733&sequence=000007&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014961733&sequence=000008&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Embryonic stem cells a practical approach Practical approach series Embryonic stem cells Stem Cells physiology Embryo physiology Mice Models, Animal Embryonale Stammzelle (DE-588)4699648-5 gnd |
| subject_GND | (DE-588)4699648-5 |
| title | Embryonic stem cells a practical approach |
| title_auth | Embryonic stem cells a practical approach |
| title_exact_search | Embryonic stem cells a practical approach |
| title_exact_search_txtP | Embryonic stem cells a practical approach |
| title_full | Embryonic stem cells a practical approach ed. by Elena Notarianni ... |
| title_fullStr | Embryonic stem cells a practical approach ed. by Elena Notarianni ... |
| title_full_unstemmed | Embryonic stem cells a practical approach ed. by Elena Notarianni ... |
| title_short | Embryonic stem cells |
| title_sort | embryonic stem cells a practical approach |
| title_sub | a practical approach |
| topic | Embryonic stem cells Stem Cells physiology Embryo physiology Mice Models, Animal Embryonale Stammzelle (DE-588)4699648-5 gnd |
| topic_facet | Embryonic stem cells Stem Cells physiology Embryo physiology Mice Models, Animal Embryonale Stammzelle |
| url | http://www.loc.gov/catdir/toc/ecip066/2006000423.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014961733&sequence=000007&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014961733&sequence=000008&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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