Metabolomics: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Schriftenreihe: | Methods in molecular biology
358 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis Klappentext |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XII, 293 S. Ill., graph. Darst. |
| ISBN: | 1588295613 |
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| 650 | 4 | |a Metabolism |x physiology |v Laboratory Manuals | |
| 650 | 4 | |a Spectrum Analysis, Mass |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Magnetic Resonance Spectroscopy |x methods |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804135538122293248 |
|---|---|
| adam_text | METABOLOMICS / WECKWERTH, WOLFRAM : C2007 TABLE OF CONTENTS /
INHALTSVERZEICHNIS METABOLITE PROFILING IN BLOOD PLASMA / OLIVER FIEHN
AND TOBIAS KIND NONSUPERVISED CONSTRUCTION AND APPLICATION OF MASS
SPECTRAL AND RETENTION TIME INDEX LIBRARIES FROM TIME-OF-FLIGHT GC/MS
METABOLITE PROFILES / ALEXANDER ERBAN ... [ET AL.] METABOLOMIC PROFILING
OF NATURAL VOLATILES: HEAD SPACE TRAPPING-GC/MS / YURY M. TIKUNOV,
FRANCEL W. A. VERSTAPPEN, AND ROBERT D. HALL INTEGRATIVE PROFILING OF
METABOLITES AND PROTEINS: IMPROVING PATTERN RECOGNITION AND BIOMARKER
SELECTION FOR SYSTEMS-LEVEL APPROACHES / KATJA MORGENTHAL ... [ET AL.]
INTEGRATING PROFILING DATA: USING LINEAR CORRELATION TO REVEAL
COREGULATION OF TRANSCRIPT AND METABOLITES / EWA URBANCZYK-WOCHNIAK,
LOTHAR WILLMITZER, AND ALISDAIR R. FERNIE VISUALIZATION AND ANALYSIS OF
MOLECULAR DATA / MATTHIAS SCHOLZ AND JOACHIM SELBIG A GENTLE GUIDE TO
THE ANALYSIS OF METABOLOMIC DATA / RALF STEUER ... [ET AL.] CE-MS FOR
METABOLOMICS / TOMOYOSHI SOGA APPLICATION OF LC/MS ANALYSIS IN
METABOLOMICS: REVERSED-PHASE MONOLITHIC CAPILLARY CHROMATOGRAPHY AND
HYDROPHILIC CHROMATOGRAPHY COUPLED TO ELECTROSPRAY IONIZATION MASS
SPECTROMETRY / VLADIMIR V. TOLSTIKOV, OLIVER FIEHN, AND NOBUO TANAKA
HPLC-SEPARATIONS COUPLED WITH COULOMETRIC-ELECTRODE ARRAY DETECTORS: A
UNIQUE APPROACH TO METABOLOMICS / BRUCE S. KRISTAL ... [ET AL.]
DETERMINATION OF METABOLIC FLUX RATIOS FROM 13C-EXPERIMENTS AND GC/MS
DATA: PROTOCOL AND PRINCIPLES / ANNIK NANCHEN, TOBIAS FUHRER, AND UWE
SAUER UNDERSTANDING THE ROADMAP OF METABOLISM BY PATHWAY ANALYSIS /
STEFAN SCHUSTER, AXEL VON KAMP, AND MIKHAIL PACHKOV REVEALING THE
METABOLOME OF ANIMAL TISSUES USING 1H NMR SPECTROSCOPY / MARK R. VIANT
NMR METABONOMICS: METHODS FOR DRUG DISCOVERY AND DEVELOPMENT /
KARL-HEINZ OTT AND NELLY ARANIBAR. DIESES SCHRIFTSTUECK WURDE
MASCHINELL ERZEUGT.
Metabolomics
Methods and Protocols
Edited by
Wolfram Weckwerth
Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology,
Potsdam-Golm, Germany
Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Lead¬
ing researchers in the field present protocols for the application of complementary analytical methods, such
as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains
forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the
structure of the unknowns detected in metabolomic studies.
Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite
profiling in blood plasma, construction and application of mass spectral and retention time index libraries,
and metabolomic profiling of natural
techniques, and specific protocols discuss improving pattern recognition and biomarker selection for
systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV,
and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase
liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC-
separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination
of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis
is provided. The volume concludes with an examination of cutting-edge NMR protocols.
GC-MS techniques and application to real-world
samples
Integration of GC-MS metabolite profiling with
protein and transcript profiling
Headspace trapping of volatile compounds
mining
metabolism
Part I Metabolome Analysis Using
Mass Spectromefry. Metabolite Profiling in Blood Plasma. Nonsuper-
vised Construction and Application of Mass Spectral and Retention
Time Index Libraries From Time-of-Flight Gas Chromatography-Mass
Spectrometry Metabolite Profiles. Metabolomic Profiling of Natural
Volatiles:
Integration and Data Mining.
Proteins: improving
Systems Level Approaches. Integrating Profiling Data: Using Linear
Correlation to Reveal Coregulation of
Visualization and Analysis of Molecular Data. A Gentle Guide to the
Analysis of Metaboiomic Data. Part III Capillary Electrophoresis Cou¬
pled
iry for Metabolomics. Part IV Liquid Chromatography Coupled
Spectrometry for Metabolomics and
of Liquid Chromatography-jVta Spectromeiry Analysis in Metabolom-
ics: Reversed-Phax Monolithic Capillary Chromatography and Hydro-
phiiic Chromatography Coupled to Elcctrospray lonization-Mass
Spectromefiy.
High-Performance Liquid Chromatography Separations Coupled
With Coulometric Electrode Array Detectors: A Unique Approach to
Metabolomics. Part VI Metabolic Fluxes. Determination of Metabolic
Flux Ratios From C-Experinients and Gas Chromatography-Mass
Spectrometry Data: Protocol and Principles. Understanding the Road-
map of Metabolism by Pathway Analysis. Part
Analysis. Revealing the Metabolome of Animal Tissues Using !H
Nuclear Magnetic Resonance Spectroscopy. Nuclear Magnetic Res¬
onance Metabonomics: Methods for Drug Discovery and Develop¬
ment Practical Aspects of Uniform Stable Isotope Labeling of Higher
Plants for Meteronudear NMR-Based Metabolomics. Index.
|
| adam_txt |
METABOLOMICS / WECKWERTH, WOLFRAM : C2007 TABLE OF CONTENTS /
INHALTSVERZEICHNIS METABOLITE PROFILING IN BLOOD PLASMA / OLIVER FIEHN
AND TOBIAS KIND NONSUPERVISED CONSTRUCTION AND APPLICATION OF MASS
SPECTRAL AND RETENTION TIME INDEX LIBRARIES FROM TIME-OF-FLIGHT GC/MS
METABOLITE PROFILES / ALEXANDER ERBAN . [ET AL.] METABOLOMIC PROFILING
OF NATURAL VOLATILES: HEAD SPACE TRAPPING-GC/MS / YURY M. TIKUNOV,
FRANCEL W. A. VERSTAPPEN, AND ROBERT D. HALL INTEGRATIVE PROFILING OF
METABOLITES AND PROTEINS: IMPROVING PATTERN RECOGNITION AND BIOMARKER
SELECTION FOR SYSTEMS-LEVEL APPROACHES / KATJA MORGENTHAL . [ET AL.]
INTEGRATING PROFILING DATA: USING LINEAR CORRELATION TO REVEAL
COREGULATION OF TRANSCRIPT AND METABOLITES / EWA URBANCZYK-WOCHNIAK,
LOTHAR WILLMITZER, AND ALISDAIR R. FERNIE VISUALIZATION AND ANALYSIS OF
MOLECULAR DATA / MATTHIAS SCHOLZ AND JOACHIM SELBIG A GENTLE GUIDE TO
THE ANALYSIS OF METABOLOMIC DATA / RALF STEUER . [ET AL.] CE-MS FOR
METABOLOMICS / TOMOYOSHI SOGA APPLICATION OF LC/MS ANALYSIS IN
METABOLOMICS: REVERSED-PHASE MONOLITHIC CAPILLARY CHROMATOGRAPHY AND
HYDROPHILIC CHROMATOGRAPHY COUPLED TO ELECTROSPRAY IONIZATION MASS
SPECTROMETRY / VLADIMIR V. TOLSTIKOV, OLIVER FIEHN, AND NOBUO TANAKA
HPLC-SEPARATIONS COUPLED WITH COULOMETRIC-ELECTRODE ARRAY DETECTORS: A
UNIQUE APPROACH TO METABOLOMICS / BRUCE S. KRISTAL . [ET AL.]
DETERMINATION OF METABOLIC FLUX RATIOS FROM 13C-EXPERIMENTS AND GC/MS
DATA: PROTOCOL AND PRINCIPLES / ANNIK NANCHEN, TOBIAS FUHRER, AND UWE
SAUER UNDERSTANDING THE ROADMAP OF METABOLISM BY PATHWAY ANALYSIS /
STEFAN SCHUSTER, AXEL VON KAMP, AND MIKHAIL PACHKOV REVEALING THE
METABOLOME OF ANIMAL TISSUES USING 1H NMR SPECTROSCOPY / MARK R. VIANT
NMR METABONOMICS: METHODS FOR DRUG DISCOVERY AND DEVELOPMENT /
KARL-HEINZ OTT AND NELLY ARANIBAR. DIESES SCHRIFTSTUECK WURDE
MASCHINELL ERZEUGT.
Metabolomics
Methods and Protocols
Edited by
Wolfram Weckwerth
Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology,
Potsdam-Golm, Germany
Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Lead¬
ing researchers in the field present protocols for the application of complementary analytical methods, such
as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains
forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the
structure of the unknowns detected in metabolomic studies.
Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite
profiling in blood plasma, construction and application of mass spectral and retention time index libraries,
and metabolomic profiling of natural
techniques, and specific protocols discuss improving pattern recognition and biomarker selection for
systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV,
and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase
liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC-
separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination
of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis
is provided. The volume concludes with an examination of cutting-edge NMR protocols.
GC-MS techniques and application to real-world
samples
Integration of GC-MS metabolite profiling with
protein and transcript profiling
Headspace trapping of volatile compounds
mining
metabolism
Part I Metabolome Analysis Using
Mass Spectromefry. Metabolite Profiling in Blood Plasma. Nonsuper-
vised Construction and Application of Mass Spectral and Retention
Time Index Libraries From Time-of-Flight Gas Chromatography-Mass
Spectrometry Metabolite Profiles. Metabolomic Profiling of Natural
Volatiles:
Integration and Data Mining.
Proteins: improving
Systems Level Approaches. Integrating Profiling Data: Using Linear
Correlation to Reveal Coregulation of
Visualization and Analysis of Molecular Data. A Gentle Guide to the
Analysis of Metaboiomic Data. Part III Capillary Electrophoresis Cou¬
pled
iry for Metabolomics. Part IV Liquid Chromatography Coupled
Spectrometry for Metabolomics and
of Liquid Chromatography-jVta Spectromeiry Analysis in Metabolom-
ics: Reversed-Phax Monolithic Capillary Chromatography and Hydro-
phiiic Chromatography Coupled to Elcctrospray lonization-Mass
Spectromefiy.
High-Performance Liquid Chromatography Separations Coupled
With Coulometric Electrode Array Detectors: A Unique Approach to
Metabolomics. Part VI Metabolic Fluxes. Determination of Metabolic
Flux Ratios From "C-Experinients and Gas Chromatography-Mass
Spectrometry Data: Protocol and Principles. Understanding the Road-
map of Metabolism by Pathway Analysis. Part
Analysis. Revealing the Metabolome of Animal Tissues Using !H
Nuclear Magnetic Resonance Spectroscopy. Nuclear Magnetic Res¬
onance Metabonomics: Methods for Drug Discovery and Develop¬
ment Practical Aspects of Uniform Stable Isotope Labeling of Higher
Plants for Meteronudear NMR-Based Metabolomics. Index. |
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| id | DE-604.BV021705011 |
| illustrated | Illustrated |
| index_date | 2024-07-02T15:18:36Z |
| indexdate | 2024-07-09T20:42:05Z |
| institution | BVB |
| isbn | 1588295613 |
| language | English |
| lccn | 2006007129 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014918918 |
| oclc_num | 64444091 |
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| physical | XII, 293 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Metabolomics methods and protocols ed. by Wolfram Weckwerth Totowa, NJ Humana Press 2007 XII, 293 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 358 Includes bibliographical references and index Metabolism Laboratory manuals Metabolism physiology Laboratory Manuals Spectrum Analysis, Mass methods Laboratory Manuals Magnetic Resonance Spectroscopy methods Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Metabolit (DE-588)4138579-2 gnd rswk-swf Stoffwechselphysiologie (DE-588)4183413-6 gnd rswk-swf Metabolit (DE-588)4138579-2 s Biochemische Methode (DE-588)4319880-6 s DE-604 Stoffwechselphysiologie (DE-588)4183413-6 s Biochemische Analyse (DE-588)4255721-5 s b DE-604 Weckwerth, Wolfram 1969- Sonstige (DE-588)120840480 oth Methods in molecular biology 358 (DE-604)BV035362695 358 http://www.loc.gov/catdir/toc/ecip069/2006007129.html Table of contents LoC Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014918918&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014918918&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Metabolomics methods and protocols Methods in molecular biology Metabolism Laboratory manuals Metabolism physiology Laboratory Manuals Spectrum Analysis, Mass methods Laboratory Manuals Magnetic Resonance Spectroscopy methods Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Biochemische Methode (DE-588)4319880-6 gnd Metabolit (DE-588)4138579-2 gnd Stoffwechselphysiologie (DE-588)4183413-6 gnd |
| subject_GND | (DE-588)4255721-5 (DE-588)4319880-6 (DE-588)4138579-2 (DE-588)4183413-6 |
| title | Metabolomics methods and protocols |
| title_auth | Metabolomics methods and protocols |
| title_exact_search | Metabolomics methods and protocols |
| title_exact_search_txtP | Metabolomics methods and protocols |
| title_full | Metabolomics methods and protocols ed. by Wolfram Weckwerth |
| title_fullStr | Metabolomics methods and protocols ed. by Wolfram Weckwerth |
| title_full_unstemmed | Metabolomics methods and protocols ed. by Wolfram Weckwerth |
| title_short | Metabolomics |
| title_sort | metabolomics methods and protocols |
| title_sub | methods and protocols |
| topic | Metabolism Laboratory manuals Metabolism physiology Laboratory Manuals Spectrum Analysis, Mass methods Laboratory Manuals Magnetic Resonance Spectroscopy methods Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Biochemische Methode (DE-588)4319880-6 gnd Metabolit (DE-588)4138579-2 gnd Stoffwechselphysiologie (DE-588)4183413-6 gnd |
| topic_facet | Metabolism Laboratory manuals Metabolism physiology Laboratory Manuals Spectrum Analysis, Mass methods Laboratory Manuals Magnetic Resonance Spectroscopy methods Laboratory Manuals Biochemische Analyse Biochemische Methode Metabolit Stoffwechselphysiologie |
| url | http://www.loc.gov/catdir/toc/ecip069/2006007129.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014918918&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014918918&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT weckwerthwolfram metabolomicsmethodsandprotocols |