Marijuana and cannabinoid research: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2006
|
| Series: | Methods in molecular medicine
123 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Physical Description: | XV, 306 S. Ill., graph. Darst. |
| ISBN: | 1588293505 9781588293503 |
Staff View
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| 245 | 1 | 0 | |a Marijuana and cannabinoid research |b methods and protocols |c ed. by Emmanuel S. Onaivi |
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Contents
Dedication
Preface
Acknowledgments
Contributors
1
Research
George R.
В.
Claire M. Leonard,
and Qing-Rong Liu
2
Cannabinoid Receptor in Immune Function
Nancy E. Buckley,
Tanya Ferguson, and Renee Y. Paau
3
Using Immunoperoxidase Methods
Guy
4
Using Ribonucleotide Probes:
Methods for Double- and Single-Label In Situ Hybridization
Andrea G. Hohmann
5
and Astroglial Cells After a Chronic CB, Agonist Treatment
Patricia
Sergio
6
and Electrophysiological Approaches
Alexander F. Hoffman and Carl
7
Ganesh A. Thakur, Spyros P. Nikas, Richard
and Alexandros Makriyannis
8
and Cannabinoid-Stimulated P^IGTPtS Binding
to Membrane
Chris
xi
x¡¡
9
in Synaptosomes
Filomena rezza,
and Mauro Maccarrone
10
on Gastrointestinal Functions
N
11
Vas Deferens
Adele
12
Craving Questionnaire
Stephen J. Heishman and Edward G. Singleton
13
on Human Associative Processes and Memory
Robert I. Block
14
David A. Gorelick and Stephen J. Heishman
15
Jean
16
Ester Fride, Alex Perchuk, F. Scott Hall, George R.
and Emmanuel S. Onaivi
17
of CB, Cannabinoid Receptors and Its Gene Transcripts
in Chronic Mild Stress Model of Depression
Emmanuel S. Onaivi, Hiroki Ishiguro,
Paul A. Meozzi, Lester Myers, Patricia
Bruce Hope, Claire M. Leonard, George R.
Alicia
Index
The revolutionary discovery that the human body and brain make their own marijuana-like substances
called endocannabinoids has transformed marijuana-cannabinoid research into a mainstream science
impacting our understanding of reproduction, development, and cell death. \nMarijuana and Cannab-
inoid Research: Methods and Protocols, prominent researchers and clinicians from around the world
detail the cutting-edge in vitro and in vivo methods they are using to elucidate the mechanisms associated
with cannabinoid function in health and disease. The techniques can be used in studies across the board
from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure,
the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their
association with polysubstance abuse. Other chapters describe methods to study the role of CBrs in
immune function, the morphometric cytoskeletal components of
cannabinoid treatment, and the in vitro and in vivo effects of cannabinoids in animals, including humans.
There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid
effects using brain slice imaging and electrophysiological approaches, and designing and synthesizing
cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Med¬
icine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the
principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshoot¬
ing and avoiding known pitfalls.
Cutting-edge and highly practical, Marijuana and Cannabinoid Research: Methods and Protocols will
help both basic and clinical investigators unravel the specific mechanisms of the marijuana-cannabinoid
actions that are of central importance in biological and therapeutic research today.
Cutting-edge techniques for marijuana-
cannabinoid research from genes to
behavior
Bioassay of cannabinoids using mouse-
isolated
Design and synthesis of cannabinoids
and endocannabinoids
Gene expression studies using rodent
and human samples
Versatile
to studying mental and neurological
disorders
Synthesis of novel cannabinoids as targets
for CNS disorders
Focus on human genes that encode
cannabinoid receptors and contribute
to addiction
Step-by-step instructions to ensure
successful results
Tricks of the trade and notes on trouble¬
shooting and avoiding known pitfalls |
| adam_txt |
Contents
Dedication
Preface
Acknowledgments
Contributors
1
Research
George R.
В.
Claire M. Leonard,
and Qing-Rong Liu
2
Cannabinoid Receptor in Immune Function
Nancy E. Buckley,
Tanya Ferguson, and Renee Y. Paau
3
Using Immunoperoxidase Methods
Guy
4
Using Ribonucleotide Probes:
Methods for Double- and Single-Label In Situ Hybridization
Andrea G. Hohmann
5
and Astroglial Cells After a Chronic CB, Agonist Treatment
Patricia
Sergio
6
and Electrophysiological Approaches
Alexander F. Hoffman and Carl
7
Ganesh A. Thakur, Spyros P. Nikas, Richard
and Alexandros Makriyannis
8
and Cannabinoid-Stimulated P^IGTPtS Binding
to Membrane
Chris
xi
x¡¡
9
in Synaptosomes
Filomena rezza,
and Mauro Maccarrone
10
on Gastrointestinal Functions
N
11
Vas Deferens
Adele
12
Craving Questionnaire
Stephen J. Heishman and Edward G. Singleton
13
on Human Associative Processes and Memory
Robert I. Block
14
David A. Gorelick and Stephen J. Heishman
15
Jean
16
Ester Fride, Alex Perchuk, F. Scott Hall, George R.
and Emmanuel S. Onaivi
17
of CB, Cannabinoid Receptors and Its Gene Transcripts
in Chronic Mild Stress Model of Depression
Emmanuel S. Onaivi, Hiroki Ishiguro,
Paul A. Meozzi, Lester Myers, Patricia
Bruce Hope, Claire M. Leonard, George R.
Alicia
Index
The revolutionary discovery that the human body and brain make their own marijuana-like substances
called endocannabinoids has transformed marijuana-cannabinoid research into a mainstream science
impacting our understanding of reproduction, development, and cell death. \nMarijuana and Cannab-
inoid Research: Methods and Protocols, prominent researchers and clinicians from around the world
detail the cutting-edge in vitro and in vivo methods they are using to elucidate the mechanisms associated
with cannabinoid function in health and disease. The techniques can be used in studies across the board
from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure,
the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their
association with polysubstance abuse. Other chapters describe methods to study the role of CBrs in
immune function, the morphometric cytoskeletal components of
cannabinoid treatment, and the in vitro and in vivo effects of cannabinoids in animals, including humans.
There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid
effects using brain slice imaging and electrophysiological approaches, and designing and synthesizing
cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Med¬
icine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the
principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshoot¬
ing and avoiding known pitfalls.
Cutting-edge and highly practical, Marijuana and Cannabinoid Research: Methods and Protocols will
help both basic and clinical investigators unravel the specific mechanisms of the marijuana-cannabinoid
actions that are of central importance in biological and therapeutic research today.
Cutting-edge techniques for marijuana-
cannabinoid research from genes to
behavior
Bioassay of cannabinoids using mouse-
isolated
Design and synthesis of cannabinoids
and endocannabinoids
Gene expression studies using rodent
and human samples
Versatile
to studying mental and neurological
disorders
Synthesis of novel cannabinoids as targets
for CNS disorders
Focus on human genes that encode
cannabinoid receptors and contribute
to addiction
Step-by-step instructions to ensure
successful results
Tricks of the trade and notes on trouble¬
shooting and avoiding known pitfalls |
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| illustrated | Illustrated |
| index_date | 2024-07-02T15:09:03Z |
| indexdate | 2025-03-19T17:00:25Z |
| institution | BVB |
| isbn | 1588293505 9781588293503 |
| language | English |
| lccn | 2005046133 |
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| physical | XV, 306 S. Ill., graph. Darst. |
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| publisher | Humana Press |
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| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Marijuana and cannabinoid research methods and protocols ed. by Emmanuel S. Onaivi Totowa, NJ Humana Press 2006 XV, 306 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 123 Cannabinoïdes - Manuels de laboratoire Marijuana - Manuels de laboratoire Cannabinoids Laboratory Manuals Cannabinoids Laboratory manuals Cannabis Laboratory Manuals Marijuana Laboratory manuals Receptors, Cannabinoid Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf Cannabinoide (DE-588)4069839-7 gnd rswk-swf Marihuana (DE-588)4037558-4 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Marihuana (DE-588)4037558-4 s Molekulare Medizin (DE-588)4543844-4 s Methode (DE-588)4038971-6 s DE-604 Cannabinoide (DE-588)4069839-7 s b DE-604 Onaivi, Emmanuel S. Sonstige oth Methods in molecular medicine 123 (DE-604)BV011334960 123 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014886290&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014886290&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Marijuana and cannabinoid research methods and protocols Methods in molecular medicine Cannabinoïdes - Manuels de laboratoire Marijuana - Manuels de laboratoire Cannabinoids Laboratory Manuals Cannabinoids Laboratory manuals Cannabis Laboratory Manuals Marijuana Laboratory manuals Receptors, Cannabinoid Laboratory Manuals Methode (DE-588)4038971-6 gnd Cannabinoide (DE-588)4069839-7 gnd Marihuana (DE-588)4037558-4 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4069839-7 (DE-588)4037558-4 (DE-588)4543844-4 |
| title | Marijuana and cannabinoid research methods and protocols |
| title_auth | Marijuana and cannabinoid research methods and protocols |
| title_exact_search | Marijuana and cannabinoid research methods and protocols |
| title_exact_search_txtP | Marijuana and cannabinoid research methods and protocols |
| title_full | Marijuana and cannabinoid research methods and protocols ed. by Emmanuel S. Onaivi |
| title_fullStr | Marijuana and cannabinoid research methods and protocols ed. by Emmanuel S. Onaivi |
| title_full_unstemmed | Marijuana and cannabinoid research methods and protocols ed. by Emmanuel S. Onaivi |
| title_short | Marijuana and cannabinoid research |
| title_sort | marijuana and cannabinoid research methods and protocols |
| title_sub | methods and protocols |
| topic | Cannabinoïdes - Manuels de laboratoire Marijuana - Manuels de laboratoire Cannabinoids Laboratory Manuals Cannabinoids Laboratory manuals Cannabis Laboratory Manuals Marijuana Laboratory manuals Receptors, Cannabinoid Laboratory Manuals Methode (DE-588)4038971-6 gnd Cannabinoide (DE-588)4069839-7 gnd Marihuana (DE-588)4037558-4 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| topic_facet | Cannabinoïdes - Manuels de laboratoire Marijuana - Manuels de laboratoire Cannabinoids Laboratory Manuals Cannabinoids Laboratory manuals Cannabis Laboratory Manuals Marijuana Laboratory manuals Receptors, Cannabinoid Laboratory Manuals Methode Cannabinoide Marihuana Molekulare Medizin |
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