Zebrafish: a practical approach
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford [u.a]
Oxford Univ. Press
2005
|
| Edition: | Reprint. |
| Series: | The practical approach series
261 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references |
| Physical Description: | XVIII, 303 S. Ill., graph. Darst. |
| ISBN: | 019963808X 0199638098 |
Staff View
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| 245 | 1 | 0 | |a Zebrafish |b a practical approach |c ed. by Christiane Nüsslein-Volhard ... |
| 250 | |a Reprint. | ||
| 264 | 1 | |a Oxford [u.a] |b Oxford Univ. Press |c 2005 | |
| 300 | |a XVIII, 303 S. |b Ill., graph. Darst. | ||
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| adam_text | Contents
List of protocols
хій
Abbreviations
xvii
Introduction: zebrafish as a system to study development and
organogénesis
ι
Christiane
Nüsslein-VóOiard,
Darren
T.
Gümour,
and
Ralf Dahm
1
History
1
2
Phylogeny
2
3
The zebrafish genome
2
4
Advantages of the zebrafish as a model system
4
5
Purpose of this book
4
Acknowledgements
S
References
5
1
Keeping and raising zebrafish
7
Michael Brand, Michael
Granato,
and
Christiane Nüsskin-
Volhard
1
Introduction
7
2
Aquaria systems and water conditions
7
Aquaria systems
7
Aquaria
10
3
Water
12
Fresh fish water
12
Filtering
14
4
Room conditions and maintenance
15
Room conditions
15
Temperature
16
Illumination
16
Cleaning
16
Safety
18
5
Fish care
18
Keeping adult fish
18
Raising fish
19
6
Maintaining stocks
28
Maintaining wild-type stocks
28
Maintaining mutant stocks
28
Identifying and maintaining adult heterozygous mutant carriers
29
Maintaining mutant stocks as frozen sperm
30
VU
CONTENTS
7
Fish health
34
Fish diseases
34
Fish handling in systems with latent
ТВ
35
Quarantine
36
References
37
2
Looking at embryos
39
Stefan
Sčhtúte-Merker
1
Introduction
39
2
in situ hybridization
39
Single probe
40
Detecting two differently labelled probes in situ
43
3
Antibody staining
45
Detecting antigens in early embryos using biotinylated secondary antibodies
45
Antibody staining of zebrafish larvae
47
Double in situ hybridization combined with antibody staining (triple stain)
48
Рте
-absorption of antibodies
50
4
Mounting
51
Viewing chambers for observing embryos
51
Methyl cellulose mounting
51
Araldite mounting
52
Mounting in benzyl benzoate/benzyl alcohol
53
5
Preparing sections
53
Plastic sections using Technovit®
54
Paraffin sections combined with antibody staining
54
Embedding and sectioning using JB-4 resin
55
Toluidine Blue staining of semi-thin sections
56
6
PTU treatment to prevent melanization of embryos
57
7
Endothelial cell staining
57
Acknowledgements
58
References
58
3
The morphology of larval and adult zebrafish
59
Thomas F. Schwing
1
Introduction
59
2
Body proportions and surface features
59
Adult
59
Larvae and juveniles
61
3
Skeleton and musculature
65
Adult skeleton
65
Larval and juvenile skeletal development
69
Adult musculature
73
Larval and juvenile muscle development
74
4
Nervous system
76
Adult central nervous system
76
Larval and juvenile neural development
79
Adult peripheral nervous system and sense organs
83
Larval and juvenile development of the
PNS
and sense organs
86
Vili
CONTENTS
5
Cardiovascular,
digestive,
and reproductive organs
87
Adult
87
Larval and juvenile
organogénesis
91
Acknowledgements
92
References
92
4
Cell labelling and transplantation techniques
95
Domad
A. Kane and Yasuyuki Kishimoto
1
Introduction
95
2
Cell labelling techniques
96
Labelling whole embryos with lineage tracer dyes
97
Labelling individual blastomeres after 25&cell stage
101
Labelling groups of cells by photo-activation
102
Labelling groups of cells by lipophilic membrane dyes
103
3
Transplantation techniques
103
Precision transplantation of small groups of cells
104
Large-scale transplantations of early blastomeres
108
Transplantation of pieces of embryos
109
4
Procedures for observing labelled cells
110
Procedures for observing
Uve
material 111
Procedures for preparing fixed material
114
Procedures for analysing data
117
References
119
5
Manipulating gene expression in the zebrafish
m
Darren T.
Gümour, Jason
R.
Jessen, and Shuo
Lin
1
Introduction
121
2
Microinjection
of zebrafish embryos
121
Equipment required
122
The microinjection procedure
125
3
Transient expression approaches
128
DNA
constructs and mRNA transcripts give different expression profiles upon injection
129
RNA
injection
129
DNA
microinjection and its applications
131
Promoter analysis
132
Co-injection
133
Rescue of mutants using transgenesis
134
Artificial chromosome transgenesis in zebrafish
134
4
Generation of stable germ line transgenic
Unes
137
Identifying transgenic founder fish by PCR
138
Identification of transgenic founder fish by reporter gene expression
139
Generation of homozygous transgenic fish
140
5
Morpholino knockdown of gene activity
141
References
143
IX
CONTENTS
6
Mutagenesis us
Francisco
Pelegri
1
Introduction
145
2
Mutagenic
agents
145
Chemical
mutagens: N-ethyl-N-nitrosourea
145
Radiation sources
149
Insertional mutagenesis
150
3
Selection of
background genetic
lines
152
General
criteria for selecting genetic backgrounds
152
Selection of lines amenable for in vitro fertilization and parthenogenesis
1520
Selection of
Unes
free of lethal or sterile mutations
153
4
Genetic screening strategies
153
Inbreeding of families carrying non-mosaic germ lines
153
Screens involving ploidy manipulation
156
Screens for recessive mutations affecting adult traits and maternal-effect mutations
167
Screens for dominant mutations
167
Alíele
screens
167
5
Screen tests
172
Morphological screens
172
Screens using molecular markers and tissue stains
172
Locomotion and behavioural screens
172
6
Conclusions
172
Acknowledgements
173
References
173
7
Mapping and cloning
175
Robert
Geisler
1
Maps of the zebrafish genome
175
2
Mapping mutations using SSLP markers
177
Mapping approaches
177
Selection of zebrafish lines and SSLPs
178
Mapping strategy
180
Set-up for high-throughput mapping
183
Evaluating pooled PCR gels
185
Evaluating single-embryo PCR gels
186
Calculation of a map position
189
Fine mapping
192
3
Radiation hybrid mapping of candidate genes
192
4
Matching mutations with candidate genes
195
Searching maps and databases
195
Evidence for the identity of a mutant locus
1%
Demonstrating a direct linkage by
SNP genotyping
196
5
Positional cloning of mutations
199
Genomic libraries
199
Screening for genomic clones
201
Analysing a genomic clone
207
Acknowledgements
210
References
210
CONTENTS
Appendix
1
List of suppliers
213
Appendix
2
Atlas of embryonic stages of development in the zebrafish
219
RalfDahm
Appendix
3
Table of zebrafish mutations
237
Hans
Georg Frohnhöfer
Index
293
Xl
|
| adam_txt |
Contents
List of protocols
хій
Abbreviations
xvii
Introduction: zebrafish as a system to study development and
organogénesis
ι
Christiane
Nüsslein-VóOiard,
Darren
T.
Gümour,
and
Ralf Dahm
1
History
1
2
Phylogeny
2
3
The zebrafish genome
2
4
Advantages of the zebrafish as a model system
4
5
Purpose of this book
4
Acknowledgements
S
References
5
1
Keeping and raising zebrafish
7
Michael Brand, Michael
Granato,
and
Christiane Nüsskin-
Volhard
1
Introduction
7
2
Aquaria systems and water conditions
7
Aquaria systems
7
Aquaria
10
3
Water
12
Fresh fish water
12
Filtering
14
4
Room conditions and maintenance
15
Room conditions
15
Temperature
16
Illumination
16
Cleaning
16
Safety
18
5
Fish care
18
Keeping adult fish
18
Raising fish
19
6
Maintaining stocks
28
Maintaining wild-type stocks
28
Maintaining mutant stocks
28
Identifying and maintaining adult heterozygous mutant carriers
29
Maintaining mutant stocks as frozen sperm
30
VU
CONTENTS
7
Fish health
34
Fish diseases
34
Fish handling in systems with latent
ТВ
35
Quarantine
36
References
37
2
Looking at embryos
39
Stefan
Sčhtúte-Merker
1
Introduction
39
2
in situ hybridization
39
Single probe
40
Detecting two differently labelled probes in situ
43
3
Antibody staining
45
Detecting antigens in early embryos using biotinylated secondary antibodies
45
Antibody staining of zebrafish larvae
47
Double in situ hybridization combined with antibody staining (triple stain)
48
Рте
-absorption of antibodies
50
4
Mounting
51
Viewing chambers for observing embryos
51
Methyl cellulose mounting
51
Araldite mounting
52
Mounting in benzyl benzoate/benzyl alcohol
53
5
Preparing sections
53
Plastic sections using Technovit®
54
Paraffin sections combined with antibody staining
54
Embedding and sectioning using JB-4 resin
55
Toluidine Blue staining of semi-thin sections
56
6
PTU treatment to prevent melanization of embryos
57
7
Endothelial cell staining
57
Acknowledgements
58
References
58
3
The morphology of larval and adult zebrafish
59
Thomas F. Schwing
1
Introduction
59
2
Body proportions and surface features
59
Adult
59
Larvae and juveniles
61
3
Skeleton and musculature
65
Adult skeleton
65
Larval and juvenile skeletal development
69
Adult musculature
73
Larval and juvenile muscle development
74
4
Nervous system
76
Adult central nervous system
76
Larval and juvenile neural development
79
Adult peripheral nervous system and sense organs
83
Larval and juvenile development of the
PNS
and sense organs
86
Vili
CONTENTS
5
Cardiovascular,
digestive,
and reproductive organs
87
Adult
87
Larval and juvenile
organogénesis
91
Acknowledgements
92
References
92
4
Cell labelling and transplantation techniques
95
Domad
A. Kane and Yasuyuki Kishimoto
1
Introduction
95
2
Cell labelling techniques
96
Labelling whole embryos with lineage tracer dyes
97
Labelling individual blastomeres after 25&cell stage
101
Labelling groups of cells by photo-activation
102
Labelling groups of cells by lipophilic membrane dyes
103
3
Transplantation techniques
103
Precision transplantation of small groups of cells
104
Large-scale transplantations of early blastomeres
108
Transplantation of pieces of embryos
109
4
Procedures for observing labelled cells
110
Procedures for observing
Uve
material 111
Procedures for preparing fixed material
114
Procedures for analysing data
117
References
119
5
Manipulating gene expression in the zebrafish
m
Darren T.
Gümour, Jason
R.
Jessen, and Shuo
Lin
1
Introduction
121
2
Microinjection
of zebrafish embryos
121
Equipment required
122
The microinjection procedure
125
3
Transient expression approaches
128
DNA
constructs and mRNA transcripts give different expression profiles upon injection
129
RNA
injection
129
DNA
microinjection and its applications
131
Promoter analysis
132
Co-injection
133
Rescue of mutants using transgenesis
134
Artificial chromosome transgenesis in zebrafish
134
4
Generation of stable germ line transgenic
Unes
137
Identifying transgenic founder fish by PCR
138
Identification of transgenic founder fish by reporter gene expression
139
Generation of homozygous transgenic fish
140
5
Morpholino 'knockdown' of gene activity
141
References
143
IX
CONTENTS
6
Mutagenesis us
Francisco
Pelegri
1
Introduction
145
2
Mutagenic
agents
145
Chemical
mutagens: N-ethyl-N-nitrosourea
145
Radiation sources
149
Insertional mutagenesis
150
3
Selection of
background genetic
lines
152
General
criteria for selecting genetic backgrounds
152
Selection of lines amenable for in vitro fertilization and parthenogenesis
1520
Selection of
Unes
free of lethal or sterile mutations
153
4
Genetic screening strategies
153
Inbreeding of families carrying non-mosaic germ lines
153
Screens involving ploidy manipulation
156
Screens for recessive mutations affecting adult traits and maternal-effect mutations
167
Screens for dominant mutations
167
Alíele
screens
167
5
Screen tests
172
Morphological screens
172
Screens using molecular markers and tissue stains
172
Locomotion and behavioural screens
172
6
Conclusions
172
Acknowledgements
173
References
173
7
Mapping and cloning
175
Robert
Geisler
1
Maps of the zebrafish genome
175
2
Mapping mutations using SSLP markers
177
Mapping approaches
177
Selection of zebrafish lines and SSLPs
178
Mapping strategy
180
Set-up for high-throughput mapping
183
Evaluating pooled PCR gels
185
Evaluating single-embryo PCR gels
186
Calculation of a map position
189
Fine mapping
192
3
Radiation hybrid mapping of candidate genes
192
4
Matching mutations with candidate genes
195
Searching maps and databases
195
Evidence for the identity of a mutant locus
1%
Demonstrating a direct linkage by
SNP genotyping
196
5
Positional cloning of mutations
199
Genomic libraries
199
Screening for genomic clones
201
Analysing a genomic clone
207
Acknowledgements
210
References
210
CONTENTS
Appendix
1
List of suppliers
213
Appendix
2
Atlas of embryonic stages of development in the zebrafish
219
RalfDahm
Appendix
3
Table of zebrafish mutations
237
Hans
Georg Frohnhöfer
Index
293
Xl |
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| dewey-full | 597.482 |
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| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | Reprint. |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV021669064 |
| illustrated | Illustrated |
| index_date | 2024-07-02T15:08:16Z |
| indexdate | 2024-07-09T20:41:14Z |
| institution | BVB |
| isbn | 019963808X 0199638098 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014883462 |
| oclc_num | 254701933 |
| open_access_boolean | |
| owner | DE-20 DE-703 |
| owner_facet | DE-20 DE-703 |
| physical | XVIII, 303 S. Ill., graph. Darst. |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Oxford Univ. Press |
| record_format | marc |
| series | The practical approach series |
| series2 | The practical approach series |
| spelling | Zebrafish a practical approach ed. by Christiane Nüsslein-Volhard ... Reprint. Oxford [u.a] Oxford Univ. Press 2005 XVIII, 303 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 261 Includes bibliographical references Versuchstiere - Zebrabärbling Genexpression (DE-588)4020136-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Embryonalentwicklung (DE-588)4070792-1 gnd rswk-swf Zebrabärbling (DE-588)4079468-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zebrabärbling (DE-588)4079468-4 s Embryonalentwicklung (DE-588)4070792-1 s Methode (DE-588)4038971-6 s DE-604 Genexpression (DE-588)4020136-3 s Nüsslein-Volhard, Christiane 1942- Sonstige (DE-588)120065916 oth The practical approach series 261 (DE-604)BV007921321 261 Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014883462&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zebrafish a practical approach The practical approach series Versuchstiere - Zebrabärbling Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4038971-6 (DE-588)4070792-1 (DE-588)4079468-4 (DE-588)4143413-4 |
| title | Zebrafish a practical approach |
| title_auth | Zebrafish a practical approach |
| title_exact_search | Zebrafish a practical approach |
| title_exact_search_txtP | Zebrafish a practical approach |
| title_full | Zebrafish a practical approach ed. by Christiane Nüsslein-Volhard ... |
| title_fullStr | Zebrafish a practical approach ed. by Christiane Nüsslein-Volhard ... |
| title_full_unstemmed | Zebrafish a practical approach ed. by Christiane Nüsslein-Volhard ... |
| title_short | Zebrafish |
| title_sort | zebrafish a practical approach |
| title_sub | a practical approach |
| topic | Versuchstiere - Zebrabärbling Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd |
| topic_facet | Versuchstiere - Zebrabärbling Genexpression Methode Embryonalentwicklung Zebrabärbling Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014883462&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV007921321 |
| work_keys_str_mv | AT nussleinvolhardchristiane zebrafishapracticalapproach |