Ubiquitin-proteasome protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in molecular biology
301 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XI, 381 S. Ill., graph. Darst. |
| ISBN: | 1588292525 |
Internformat
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| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2005 | |
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| 650 | 4 | |a Proteolytic enzymes |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804135458542714880 |
|---|---|
| adam_text | Contents
Preface
Contributors
Part I Biochemical Methods to Study the Ubiquitin-Proteasome System
1
Maria Gaczynska and
2
Arthur L. Haas
3
Manabu Furukawa, Paul S. Andrews, and Yue Xiong
4
Shahri Raasi and
5
and Proteasome-Associated Proteins From Budding Yeast
Davids. Leggett, Michael H. Glickman, and Daniel
6
the 19S Regulatory Complex of the 26S Proteasome
Chang-Wei Liu, Elizabeth Strickland, George
and Philip J. Thomas
7
Protein Degradation
Chaim Kahana and Yuval Reiss
8
Stefan
9
Carol E. Parker, Maria R. Warren, David R. Loiselle, Nedyalka
Dicheva, Cameron O. Scarlett, and
10
Carol E. Parker, Viorel Mocanu, Maria R. Warren, Susanna F.
Greer, and
11
Using Yeast Membranes and Cytosol
Robert J. Lee, Ardythe A. McCracken, and Jeffrey L.
vii
vìii
12
Reticulum Membrane Protein Degradation
Eric Carlson, Nathan Bays, Larry David, and
13
and Characterization
Nathaniels. Russell and Keith D. Wilkinson
Part II Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions
14
Edward
15
R.
and Paula C. Ramos
16
Aaron Ciechanover
17
Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell
18
and Degradation: Screen for
Sulfonate Mutagenesis
Antje Schäfer
19
and Degradation: Genome Wide Screen for
Antje Schäfer
20
Substrate to Study Endoplasmic Reticulum Protein Quality Control
in Mammalian Cells
/.
Meredith F.
21
Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley
22
RolandS. Hilgarth and Kevin D.
23
Robert Z. Orlowski
24
of Neural Parkin
Michael G.
Index
The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with
the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart
disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many
of whom originally developed the methods they describe, detail cutting-edge techniques for studying
ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic
biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochem¬
ical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-protea-
some-dependent functions. The protocols follow the successful Methods in Molecular Biology7 series
format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the
technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known
pitfalls.
State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced
bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate
discovery, enhance productivity, and permit manipulation of the system for varied research purposes.
Techniques for exploring ubiquitin-dependent
protein degradation via the proteasome
Cutting-edge methods to study the ubiquitin-
proteasome system and dependent functions aiKj avoiding known pitfalls
|
| adam_txt |
Contents
Preface
Contributors
Part I Biochemical Methods to Study the Ubiquitin-Proteasome System
1
Maria Gaczynska and
2
Arthur L. Haas
3
Manabu Furukawa, Paul S. Andrews, and Yue Xiong
4
Shahri Raasi and
5
and Proteasome-Associated Proteins From Budding Yeast
Davids. Leggett, Michael H. Glickman, and Daniel
6
the 19S Regulatory Complex of the 26S Proteasome
Chang-Wei Liu, Elizabeth Strickland, George
and Philip J. Thomas
7
Protein Degradation
Chaim Kahana and Yuval Reiss
8
Stefan
9
Carol E. Parker, Maria R. Warren, David R. Loiselle, Nedyalka
Dicheva, Cameron O. Scarlett, and
10
Carol E. Parker, Viorel Mocanu, Maria R. Warren, Susanna F.
Greer, and
11
Using Yeast Membranes and Cytosol
Robert J. Lee, Ardythe A. McCracken, and Jeffrey L.
vii
vìii
12
Reticulum Membrane Protein Degradation
Eric Carlson, Nathan Bays, Larry David, and
13
and Characterization
Nathaniels. Russell and Keith D. Wilkinson
Part II Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions
14
Edward
15
R.
and Paula C. Ramos
16
Aaron Ciechanover
17
Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell
18
and Degradation: Screen for
Sulfonate Mutagenesis
Antje Schäfer
19
and Degradation: Genome Wide Screen for
Antje Schäfer
20
Substrate to Study Endoplasmic Reticulum Protein Quality Control
in Mammalian Cells
/.
Meredith F.
21
Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley
22
RolandS. Hilgarth and Kevin D.
23
Robert Z. Orlowski
24
of Neural Parkin
Michael G.
Index
The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with
the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart
disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many
of whom originally developed the methods they describe, detail cutting-edge techniques for studying
ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic
biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochem¬
ical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-protea-
some-dependent functions. The protocols follow the successful Methods in Molecular Biology7" series
format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the
technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known
pitfalls.
State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced
bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate
discovery, enhance productivity, and permit manipulation of the system for varied research purposes.
Techniques for exploring ubiquitin-dependent
protein degradation via the proteasome
Cutting-edge methods to study the ubiquitin-
proteasome system and dependent functions aiKj avoiding known pitfalls |
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| spelling | Ubiquitin-proteasome protocols ed. by Cam Patterson ... Totowa, NJ Humana Press 2005 XI, 381 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 301 Biologia molecular larpcal Enzimas (metabolismo) larpcal Manuais de laboratório larpcal Peptídeos (enzimologia) larpcal Ubiquitin Laboratory manuals Proteolytic enzymes Laboratory manuals Ubiquitin metabolism Cysteine Endopeptidases Multienzyme Complexes Biologisches Verfahren (DE-588)4361950-2 gnd rswk-swf Ubiquitin (DE-588)4314848-7 gnd rswk-swf Proteasom (DE-588)4315827-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ubiquitin (DE-588)4314848-7 s Proteasom (DE-588)4315827-4 s Biologisches Verfahren (DE-588)4361950-2 s b DE-604 Patterson, Cam Sonstige oth Methods in molecular biology 301 (DE-604)BV035362695 301 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866388&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866388&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Ubiquitin-proteasome protocols Methods in molecular biology Biologia molecular larpcal Enzimas (metabolismo) larpcal Manuais de laboratório larpcal Peptídeos (enzimologia) larpcal Ubiquitin Laboratory manuals Proteolytic enzymes Laboratory manuals Ubiquitin metabolism Cysteine Endopeptidases Multienzyme Complexes Biologisches Verfahren (DE-588)4361950-2 gnd Ubiquitin (DE-588)4314848-7 gnd Proteasom (DE-588)4315827-4 gnd |
| subject_GND | (DE-588)4361950-2 (DE-588)4314848-7 (DE-588)4315827-4 (DE-588)4143413-4 |
| title | Ubiquitin-proteasome protocols |
| title_auth | Ubiquitin-proteasome protocols |
| title_exact_search | Ubiquitin-proteasome protocols |
| title_exact_search_txtP | Ubiquitin-proteasome protocols |
| title_full | Ubiquitin-proteasome protocols ed. by Cam Patterson ... |
| title_fullStr | Ubiquitin-proteasome protocols ed. by Cam Patterson ... |
| title_full_unstemmed | Ubiquitin-proteasome protocols ed. by Cam Patterson ... |
| title_short | Ubiquitin-proteasome protocols |
| title_sort | ubiquitin proteasome protocols |
| topic | Biologia molecular larpcal Enzimas (metabolismo) larpcal Manuais de laboratório larpcal Peptídeos (enzimologia) larpcal Ubiquitin Laboratory manuals Proteolytic enzymes Laboratory manuals Ubiquitin metabolism Cysteine Endopeptidases Multienzyme Complexes Biologisches Verfahren (DE-588)4361950-2 gnd Ubiquitin (DE-588)4314848-7 gnd Proteasom (DE-588)4315827-4 gnd |
| topic_facet | Biologia molecular Enzimas (metabolismo) Manuais de laboratório Peptídeos (enzimologia) Ubiquitin Laboratory manuals Proteolytic enzymes Laboratory manuals Ubiquitin metabolism Cysteine Endopeptidases Multienzyme Complexes Biologisches Verfahren Ubiquitin Proteasom Aufsatzsammlung |
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