Short protocols in neuroscience: cellular and molecular methods ; a compendium of methods from current protocols in neuroscience
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Hoboken, NJ
Wiley
2006
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | getr. Zählung graph. Darst. |
| ISBN: | 9780471783992 0471783994 |
Staff View
MARC
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| 245 | 1 | 0 | |a Short protocols in neuroscience |b cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |c ed. board Charles R. Gerfen ... |
| 264 | 1 | |a Hoboken, NJ |b Wiley |c 2006 | |
| 300 | |a getr. Zählung |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Cytology |v Laboratory manuals | |
| 650 | 4 | |a Gene Expression Regulation |x physiology |v Laboratory Manuals | |
| 650 | 4 | |a Molecular neurobiology |v Laboratory manuals | |
| 650 | 4 | |a Nervous System Physiological Phenomena |v Laboratory Manuals | |
| 650 | 4 | |a Neurobiology |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Neurons |v Laboratory manuals | |
| 650 | 4 | |a Neurons |x physiology |v Laboratory Manuals | |
| 650 | 4 | |a Neurotransmitter Agents |x physiology |v Laboratory Manuals | |
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| adam_text | CONTENTS
Preface
Contributors
1
1.1
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Coating of Slide-Mounted Sections for Autoradiography
Support Protocol: Preparation of Gelatin-Subbed Microscope Slides
1.2
Basic Protocol
Basic Protocol
Basic Protocol
Microscopy
1.3
Basic Protocol
Probes
Basic Protocol
Basic Protocol
AP-Conjugated Anti-Digoxigenin Antibodies
Alternate Protocol: Tyramide Signal Amplification (TSA) Detection of
Digoxigenin-Labeled Probes
Basic Protocol
Riboprobes
Basic Protocol
Support Protocol
Histochemistry
Support Protocol
Histochemistry
1.4
Radioligand Selection
Characterization of Ligand-Binding Assays
Quantification of Ligand Binding
Analyses of
Conclusion
1.5
Basic Protocol
Alternate Protocol: Intracellular Biocytin Staining with Patch Electrodes
Basic Protocol
Support Protocol: Tissue Processing
2
2.1
Immunolabeling: General Steps for Labeling Fixed Cells and Tissues
2.2
Basic Protocol
Contents Cellular and Molecular Methods
Basic
Immunogold Electron Microscopy
Support Protocol
Basic Protocol
Cultured Hippocampal Neurons
Support Protocol
Brain
2.3
The Basis of Optical Sectioning
Types of Confocal Microscopes
Practical Guidelines
2.4
Basic Protocol
Fluorescent Indicators (Acetoxymethyl-AM-Ester Dyes) to Cultured
Neurons
Alternate Protocol
Calcium-Sensitive Dyes to En-Bloc Nervous System Preparations or
Tissue Slices
Basic Protocol
or Slice Preparations by
Alternate Protocol
En-Bloc or Slice Preparations Using a Suction Electrode
Basic Protocol
or Slice Preparations by
3
3.1
Basic Protocol: Fetal Rat Brain Neuroepithelial Stem Cell Culture
Alternate Protocol: Adult Rodent Brain Neuroepithelial Stem Cell Culture
Support Protocol: Preparation of Polyornithine/Fibronectin-Coated Tissue
Culture Dishes
3.2
Basic Protocol: Differentiation of Embryonic Stem Cells
Support Protocol
Support Protocol
3.3
Basic Protocol
Basic Protocol
Support Protocol
Support Protocol
3.4
Basic Protocol: Culture of Hippocampal Neurons from Embryonic Day
or Mouse
Alternate Protocol: Culture of Hippocampal Neurons from Transgenic Mouse
Embryos
Support Protocol: Polyornithine/Fibronectin-Coated
3.5
Basic Protocol: Preparation of Dissociated Nigral Cell Cultures
Alternate Protocol
Alternate Protocol
3.6
Basic Protocol: Isolation and Culture of Astrocytes from Rodent Cerebellum
Alternate Protocol: Enrichment of Astrocytes by Differential Adhesion
Support Protocol: Immunohistochemical Detection of Glial Fibrillary Acidic
Protein (GFAP)
Cellular and Molecular Methods
iv
3.7
Mammalian Brain
Strategic Planning
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol
Support Protocol
Support Protocol
Support Protocol
4
4.1
Basic Protocol
Basic Protocol
Known Sequence
Basic Protocol
Sequence
4.2
Basic Protocol
Basic Protocol
Alternate Protocol
Alternate Protocol
Support Protocol
Support Protocol
43
Basic Protocol
Support Protocol
Expiants
Basic Protocol
Support Protocol
Truncated cDNA Libraries
4.4
Choice of Expression Vector
Choice of Cell Type
Introducing
Transient or Stable Gene Expression
Choice of Promoter
4.5
Strategic Planning
Basic Protocol
Support Protocol
Basic Protocol
Basic Protocol
Support Protocol
4.6
Basic Protocol
Basic Protocol
Genes in S2 Cell Lines
Support Protocol
Support Protocol
M3 Medium
4.7
Basic Protocol
Basic Protocol
Contents Cellular and Molecular Methods
Basic
Alternate
Support Protocol:
4.8
Basic Protocol
Support Protocol
Vectors
Support Protocol
Support Protocol
Assays for
(2)
Basic Protocol
Culture
Support Protocol
Cultures
Support Protocol
Ventral-Mesencephalic Cultures
Support Protocol
Support Protocol
Infected
Staining,
(3)
4.9
Basic Protocol
of Adult Rats
Basic Protocol
Vector-Mediated
Recombinant
Alternate Protocol
Brain Sections
Basic Protocol
Demyelination with Luxol Fast Blue and Cresyl Violet
Basic Protocol
Staining
Basic Protocol
Basic Protocol
Brain
Basic Protocol
Intradermal
Support Protocol
Support Protocol
Support Protocol
4.10
Basic Protocol: Production of High-Titer
Transient Transfection of 293T Cells
Support Protocol
Support Protocol
4.11
Vitro and In Vivo Administration
Basic Protocol
Transfection of
Alternate Protocol: rAAV Purification Using Heparin Sepharose Column
Purification
Support Protocol
Support Protocol
of
Support Protocol
Basic Protocol
Support Protocol
Fluorescent Protein Expression
Cellular and Molecular Methods
vi
4.12 Alphavirus-Mediated Gene Transfer
Basic
Basic Protocol
Basic Protocol
Basic Protocol
Alternate Protocol: Lipid-Mediated Cotransfection of
Support Protocol
Support Protocol
4.13
Biology of
Development of Amplicon and Genomic HSV-1 Vectors
Advantages and Disadvantages of Present-Day Amplicon Vectors
Comparison of HSV-1 with Other Vectors for Gene Transfer into Neurons
4.14
Basic Protocol
Support Protocol
Basic Protocol
Support Protocol
4.15
Basic Protocol: Preparation of Helper Virus-Free Amplicon Stocks
Support Protocol
Support Protocol
4.16
What are Microarrays Good For?
What Else are Nucleic Acid Microarrays Good For?
What About Data Analysis?
Where Can I Get More Information?
4.17
Strategic Planning
Basic Protocol: Amplification of mRNA for Expression Monitoring and
Hybridization to Oligonucleotide Array Chips
Support Protocol
of Transcript Pools
Alternate Protocol: Solid-Phase Reversible Immobilization (SPRI)
Purification of cDNA and IVT Products
Support Protocol
4.18
Strategic Planning
Basic Protocol
Alternate Protocol
Basic Protocol
Signal Amplification (TSA)
Alternate Protocol
Amplification
4.19
Anatomy of Targeting Constructs
Methods of Enrichment for Homologous
Types of Mutations
CRE/loxP System
5
5.1
Basic Protocol: Northern Hybridization of
Agarose-Formaldehyde Gel Electrophoresis
5-2
5-2
Contents
Cellular and Molecular Methods
vii
Altemate
Glyoxal/DMSO Treatment
Alternate Protocol
Immobilized by Slot Blotting
Support Protocol: Removal of Probes from Northern Blots
5.2
Basic Protocol
Alternate Protocol: Small-Volume RNase Protection Assay
Support Protocol
Support Protocol
Support Protocol
Basic Protocol
Single-Stranded
Support Protocol
Double-Stranded Plasmid or PCR Product Using Klenow Fragment
Support Protocol
Double-Stranded Plasmid or PCR Product in a Thermal Cycler Using
Thermostable Polymerase
Support Protocol
Labeling of Oligonucleotides
Support Protocol
5.3
Central Nervous System (CNS)
Basic Protocol: Single-Cell mRNA Amplification from Live Cells
Alternate Protocol: Single-Cell mRNA Amplification from Immunostained
Fixed Cells
Support Protocol
Support Protocol
5.4
History
Natural Roles of RNAi and Related Processes
Strategies for RNAi and Cosuppression in Plants and Animals
Detection and Characterization of siRNAs and miRNAs
5.5
Basic Protocol
Alternate Protocol
Glutaraldehyde
Alternate Protocol
EDCI
Alternate Protocol
BDB
Support Protocol
Support Protocol
Support Protocol
Rabbits
Support Protocol
Support Protocol
Basic Protocol
5.6
Basic Protocol
Expression Plasmid
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol
Protein
Cellular and Molecular Methods
viii
Support
Immunization
Support Protocol
Proteins
5.7
Basic Protocol
Alternate Protocol
Alternate Protocol
Support Protocol
Basic Protocol
Antibody
Alternate Protocol
Secondary Antibody
Basic Protocol
Alternate Protocol
Support Protocol
5.8
Filamentous Phage Biology
Phage Display
Vector Systems Used for Phage Display
Use of Phage Display Libraries to Map Protein Binding Sites
Displaying cDNA and Genomic Libraries
Selecting Antibodies by Phage Display
Improving Affinity
Using Phage Antibodies as a Discovery Tool
Disease-Specific Phage Antibody Libraries
Displaying Other Proteins
Specific Phage Display Applications in Neurology
5.9
Basic Protocol
Precipitation
Support Protocol
Basic Protocol
Basic Protocol
Indirectly or Directly on (Plastic) Surfaces
Alternate Protocol: Selection of Phage Antibodies Using Biotinylated
Antigen and Streptavidin-Paramagnetic Beads
Support Protocol
Support Protocol
Testing
Basic Protocol
Support Protocol
Basic Protocol
Basic Protocol
Support Protocol
5.10
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol: Phosphorylation of Fusion Proteins In Vitro
5.11
Selection of Detergent
Evaluation of Solubilization Conditions
Solubilization of m2 Muscarinic Receptors
Conclusions
Contents
ix
С ПО
5.12
Basic Protocol
Nondenaturing Detergent Solution
Alternate Protocol
Nondenaturing Detergent Solution
Alternate Protocol
Under Denaturing Conditions
Alternate Protocol
Alternate Protocol
Support Protocol: Preparation of Antibody-Sepharose
Alternate Protocol
Anti-Ig Serum
Basic Protocol
5.13
Basic Protocol: Coprecipitating Proteins with Protein A- or Protein
G-Sepharose
Alternate Protocol: Coprecipitating a GST Fusion Protein
5.14
Transfer (FRET) Microscopy
Basic Protocol: FRET Microscopy of Fixed Cells
Support Protocol
Support Protocol
5.15
Research
Transgenic Mice: Some General Considerations
ВАС
BAC Modification
Characterization of Modified BACs and Preparation of
Microinjections
Mouse Strain Considerations
Applications of
5.16
of Intact
Basic Protocol: Modification of BACs Using the pLD53.SC-
Alternate Protocol: Preparation of
CL-4B Chromatography
Appendices
1
2
2
2B Optimization of Transfection A2-4
Calcium Phosphate Transfection A2-4
DEAE-Dextran Transfection A2-5
Electroporation A2-6
Liposome-Mediated Transfection A2-6
2C Purification and Concentration of
Basic Protocol: Phenol Extraction and
Alternate Protocol
Support Protocol
Support Protocol
Butanol
Alternate Protocol
Alternate Protocol
Dilute Solutions of
Alternate Protocol
Oligonucleotides and Triphosphates by
Cellular and Molecular Methods
2D
Basic Protocol: Preparation of Genomic
2Б
Strategic Planning A2-13
Basic Protocol
Basic Protocol
Culture Cells A2-14
Support Protocol: Removal of Contaminating
Preparation A2-15
Basic Protocol
Alternate Protocol: Single-Step
Tissues A2-17
Basic Protocol
2F Preparation of Bacterial Plasmid
Basic Protocol
Basic Protocol
Basic Protocol
Centrifugation A2-21
Support Protocol
Support Protocol
Support Protocol
2G Quantitation of
Spectroscopy A2-24
Basic Protocol: Detection of Nucleic Acids Using Absorption
Spectroscopy A2-24
Alternate Protocol
Fluorochrome
Alternate Protocol
Bromide Fluorescence A2-26
2H Introduction of Plasmid
Basic Protocol: Transformation Using Calcium Chloride A2-27
Alternate Protocol: One-Step Preparation and Transformation
of Competent Cells A2-28
21
Aseptic Technique A2-29
Preparing Culture Medium A2-30
Basic Protocol: Trypsinizing and Subculturing Cells from a Monolayer A2-32
Support Protocol
Cultures A2-33
Support Protocol
Support Protocol
Support Protocol
Hemacytometer and Trypan Blue Staining A2-35
Support Protocol
Selected Suppliers of Reagents and Equipment A3-1
References
Index
Contents
xi
|
| adam_txt |
CONTENTS
Preface
Contributors
1
1.1
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Basic Protocol
Coating of Slide-Mounted Sections for Autoradiography
Support Protocol: Preparation of Gelatin-Subbed Microscope Slides
1.2
Basic Protocol
Basic Protocol
Basic Protocol
Microscopy
1.3
Basic Protocol
Probes
Basic Protocol
Basic Protocol
AP-Conjugated Anti-Digoxigenin Antibodies
Alternate Protocol: Tyramide Signal Amplification (TSA) Detection of
Digoxigenin-Labeled Probes
Basic Protocol
Riboprobes
Basic Protocol
Support Protocol
Histochemistry
Support Protocol
Histochemistry
1.4
Radioligand Selection
Characterization of Ligand-Binding Assays
Quantification of Ligand Binding
Analyses of
Conclusion
1.5
Basic Protocol
Alternate Protocol: Intracellular Biocytin Staining with Patch Electrodes
Basic Protocol
Support Protocol: Tissue Processing
2
2.1
Immunolabeling: General Steps for Labeling Fixed Cells and Tissues
2.2
Basic Protocol
Contents Cellular and Molecular Methods
Basic
Immunogold Electron Microscopy
Support Protocol
Basic Protocol
Cultured Hippocampal Neurons
Support Protocol
Brain
2.3
The Basis of Optical Sectioning
Types of Confocal Microscopes
Practical Guidelines
2.4
Basic Protocol
Fluorescent Indicators (Acetoxymethyl-AM-Ester Dyes) to Cultured
Neurons
Alternate Protocol
Calcium-Sensitive Dyes to En-Bloc Nervous System Preparations or
Tissue Slices
Basic Protocol
or Slice Preparations by
Alternate Protocol
En-Bloc or Slice Preparations Using a Suction Electrode
Basic Protocol
or Slice Preparations by
3
3.1
Basic Protocol: Fetal Rat Brain Neuroepithelial Stem Cell Culture
Alternate Protocol: Adult Rodent Brain Neuroepithelial Stem Cell Culture
Support Protocol: Preparation of Polyornithine/Fibronectin-Coated Tissue
Culture Dishes
3.2
Basic Protocol: Differentiation of Embryonic Stem Cells
Support Protocol
Support Protocol
3.3
Basic Protocol
Basic Protocol
Support Protocol
Support Protocol
3.4
Basic Protocol: Culture of Hippocampal Neurons from Embryonic Day
or Mouse
Alternate Protocol: Culture of Hippocampal Neurons from Transgenic Mouse
Embryos
Support Protocol: Polyornithine/Fibronectin-Coated
3.5
Basic Protocol: Preparation of Dissociated Nigral Cell Cultures
Alternate Protocol
Alternate Protocol
3.6
Basic Protocol: Isolation and Culture of Astrocytes from Rodent Cerebellum
Alternate Protocol: Enrichment of Astrocytes by Differential Adhesion
Support Protocol: Immunohistochemical Detection of Glial Fibrillary Acidic
Protein (GFAP)
Cellular and Molecular Methods
iv
3.7
Mammalian Brain
Strategic Planning
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol
Support Protocol
Support Protocol
Support Protocol
4
4.1
Basic Protocol
Basic Protocol
Known Sequence
Basic Protocol
Sequence
4.2
Basic Protocol
Basic Protocol
Alternate Protocol
Alternate Protocol
Support Protocol
Support Protocol
43
Basic Protocol
Support Protocol
Expiants
Basic Protocol
Support Protocol
Truncated cDNA Libraries
4.4
Choice of Expression Vector
Choice of Cell Type
Introducing
Transient or Stable Gene Expression
Choice of Promoter
4.5
Strategic Planning
Basic Protocol
Support Protocol
Basic Protocol
Basic Protocol
Support Protocol
4.6
Basic Protocol
Basic Protocol
Genes in S2 Cell Lines
Support Protocol
Support Protocol
M3 Medium
4.7
Basic Protocol
Basic Protocol
Contents Cellular and Molecular Methods
Basic
Alternate
Support Protocol:
4.8
Basic Protocol
Support Protocol
Vectors
Support Protocol
Support Protocol
Assays for
(2)
Basic Protocol
Culture
Support Protocol
Cultures
Support Protocol
Ventral-Mesencephalic Cultures
Support Protocol
Support Protocol
Infected
Staining,
(3)
4.9
Basic Protocol
of Adult Rats
Basic Protocol
Vector-Mediated
Recombinant
Alternate Protocol
Brain Sections
Basic Protocol
Demyelination with Luxol Fast Blue and Cresyl Violet
Basic Protocol
Staining
Basic Protocol
Basic Protocol
Brain
Basic Protocol
Intradermal
Support Protocol
Support Protocol
Support Protocol
4.10
Basic Protocol: Production of High-Titer
Transient Transfection of 293T Cells
Support Protocol
Support Protocol
4.11
Vitro and In Vivo Administration
Basic Protocol
Transfection of
Alternate Protocol: rAAV Purification Using Heparin Sepharose Column
Purification
Support Protocol
Support Protocol
of
Support Protocol
Basic Protocol
Support Protocol
Fluorescent Protein Expression
Cellular and Molecular Methods
vi
4.12 Alphavirus-Mediated Gene Transfer
Basic
Basic Protocol
Basic Protocol
Basic Protocol
Alternate Protocol: Lipid-Mediated Cotransfection of
Support Protocol
Support Protocol
4.13
Biology of
Development of Amplicon and Genomic HSV-1 Vectors
Advantages and Disadvantages of Present-Day Amplicon Vectors
Comparison of HSV-1 with Other Vectors for Gene Transfer into Neurons
4.14
Basic Protocol
Support Protocol
Basic Protocol
Support Protocol
4.15
Basic Protocol: Preparation of Helper Virus-Free Amplicon Stocks
Support Protocol
Support Protocol
4.16
What are Microarrays Good For?
What Else are Nucleic Acid Microarrays Good For?
What About Data Analysis?
Where Can I Get More Information?
4.17
Strategic Planning
Basic Protocol: Amplification of mRNA for Expression Monitoring and
Hybridization to Oligonucleotide Array Chips
Support Protocol
of Transcript Pools
Alternate Protocol: Solid-Phase Reversible Immobilization (SPRI)
Purification of cDNA and IVT Products
Support Protocol
4.18
Strategic Planning
Basic Protocol
Alternate Protocol
Basic Protocol
Signal Amplification (TSA)
Alternate Protocol
Amplification
4.19
Anatomy of Targeting Constructs
Methods of Enrichment for Homologous
Types of Mutations
CRE/loxP System
5
5.1
Basic Protocol: Northern Hybridization of
Agarose-Formaldehyde Gel Electrophoresis
5-2
5-2
Contents
Cellular and Molecular Methods
vii
Altemate
Glyoxal/DMSO Treatment
Alternate Protocol
Immobilized by Slot Blotting
Support Protocol: Removal of Probes from Northern Blots
5.2
Basic Protocol
Alternate Protocol: Small-Volume RNase Protection Assay
Support Protocol
Support Protocol
Support Protocol
Basic Protocol
Single-Stranded
Support Protocol
Double-Stranded Plasmid or PCR Product Using Klenow Fragment
Support Protocol
Double-Stranded Plasmid or PCR Product in a Thermal Cycler Using
Thermostable Polymerase
Support Protocol
Labeling of Oligonucleotides
Support Protocol
5.3
Central Nervous System (CNS)
Basic Protocol: Single-Cell mRNA Amplification from Live Cells
Alternate Protocol: Single-Cell mRNA Amplification from Immunostained
Fixed Cells
Support Protocol
Support Protocol
5.4
History
Natural Roles of RNAi and Related Processes
Strategies for RNAi and Cosuppression in Plants and Animals
Detection and Characterization of siRNAs and miRNAs
5.5
Basic Protocol
Alternate Protocol
Glutaraldehyde
Alternate Protocol
EDCI
Alternate Protocol
BDB
Support Protocol
Support Protocol
Support Protocol
Rabbits
Support Protocol
Support Protocol
Basic Protocol
5.6
Basic Protocol
Expression Plasmid
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol
Protein
Cellular and Molecular Methods
viii
Support
Immunization
Support Protocol
Proteins
5.7
Basic Protocol
Alternate Protocol
Alternate Protocol
Support Protocol
Basic Protocol
Antibody
Alternate Protocol
Secondary Antibody
Basic Protocol
Alternate Protocol
Support Protocol
5.8
Filamentous Phage Biology
Phage Display
Vector Systems Used for Phage Display
Use of Phage Display Libraries to Map Protein Binding Sites
Displaying cDNA and Genomic Libraries
Selecting Antibodies by Phage Display
Improving Affinity
Using Phage Antibodies as a Discovery Tool
Disease-Specific Phage Antibody Libraries
Displaying Other Proteins
Specific Phage Display Applications in Neurology
5.9
Basic Protocol
Precipitation
Support Protocol
Basic Protocol
Basic Protocol
Indirectly or Directly on (Plastic) Surfaces
Alternate Protocol: Selection of Phage Antibodies Using Biotinylated
Antigen and Streptavidin-Paramagnetic Beads
Support Protocol
Support Protocol
Testing
Basic Protocol
Support Protocol
Basic Protocol
Basic Protocol
Support Protocol
5.10
Basic Protocol
Basic Protocol
Basic Protocol
Support Protocol: Phosphorylation of Fusion Proteins In Vitro
5.11
Selection of Detergent
Evaluation of Solubilization Conditions
Solubilization of m2 Muscarinic Receptors
Conclusions
Contents
ix
С ПО
5.12
Basic Protocol
Nondenaturing Detergent Solution
Alternate Protocol
Nondenaturing Detergent Solution
Alternate Protocol
Under Denaturing Conditions
Alternate Protocol
Alternate Protocol
Support Protocol: Preparation of Antibody-Sepharose
Alternate Protocol
Anti-Ig Serum
Basic Protocol
5.13
Basic Protocol: Coprecipitating Proteins with Protein A- or Protein
G-Sepharose
Alternate Protocol: Coprecipitating a GST Fusion Protein
5.14
Transfer (FRET) Microscopy
Basic Protocol: FRET Microscopy of Fixed Cells
Support Protocol
Support Protocol
5.15
Research
Transgenic Mice: Some General Considerations
ВАС
BAC Modification
Characterization of Modified BACs and Preparation of
Microinjections
Mouse Strain Considerations
Applications of
5.16
of Intact
Basic Protocol: Modification of BACs Using the pLD53.SC-
Alternate Protocol: Preparation of
CL-4B Chromatography
Appendices
1
2
2
2B Optimization of Transfection A2-4
Calcium Phosphate Transfection A2-4
DEAE-Dextran Transfection A2-5
Electroporation A2-6
Liposome-Mediated Transfection A2-6
2C Purification and Concentration of
Basic Protocol: Phenol Extraction and
Alternate Protocol
Support Protocol
Support Protocol
Butanol
Alternate Protocol
Alternate Protocol
Dilute Solutions of
Alternate Protocol
Oligonucleotides and Triphosphates by
Cellular and Molecular Methods
2D
Basic Protocol: Preparation of Genomic
2Б
Strategic Planning A2-13
Basic Protocol
Basic Protocol
Culture Cells A2-14
Support Protocol: Removal of Contaminating
Preparation A2-15
Basic Protocol
Alternate Protocol: Single-Step
Tissues A2-17
Basic Protocol
2F Preparation of Bacterial Plasmid
Basic Protocol
Basic Protocol
Basic Protocol
Centrifugation A2-21
Support Protocol
Support Protocol
Support Protocol
2G Quantitation of
Spectroscopy A2-24
Basic Protocol: Detection of Nucleic Acids Using Absorption
Spectroscopy A2-24
Alternate Protocol
Fluorochrome
Alternate Protocol
Bromide Fluorescence A2-26
2H Introduction of Plasmid
Basic Protocol: Transformation Using Calcium Chloride A2-27
Alternate Protocol: One-Step Preparation and Transformation
of Competent Cells A2-28
21
Aseptic Technique A2-29
Preparing Culture Medium A2-30
Basic Protocol: Trypsinizing and Subculturing Cells from a Monolayer A2-32
Support Protocol
Cultures A2-33
Support Protocol
Support Protocol
Support Protocol
Hemacytometer and Trypan Blue Staining A2-35
Support Protocol
Selected Suppliers of Reagents and Equipment A3-1
References
Index
Contents
xi |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV021599741 |
| callnumber-first | Q - Science |
| callnumber-label | QP356 |
| callnumber-raw | QP356.2 |
| callnumber-search | QP356.2 |
| callnumber-sort | QP 3356.2 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4900 |
| ctrlnum | (OCoLC)68980851 (DE-599)BVBBV021599741 |
| dewey-full | 573.8/519 573.8/4 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.8/519 573.8/4 |
| dewey-search | 573.8/519 573.8/4 |
| dewey-sort | 3573.8 3519 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Book |
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| id | DE-604.BV021599741 |
| illustrated | Illustrated |
| index_date | 2024-07-02T14:47:16Z |
| indexdate | 2024-07-09T20:39:36Z |
| institution | BVB |
| isbn | 9780471783992 0471783994 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014815095 |
| oclc_num | 68980851 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | getr. Zählung graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| record_format | marc |
| spelling | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience ed. board Charles R. Gerfen ... Hoboken, NJ Wiley 2006 getr. Zählung graph. Darst. txt rdacontent n rdamedia nc rdacarrier Cytology Laboratory manuals Gene Expression Regulation physiology Laboratory Manuals Molecular neurobiology Laboratory manuals Nervous System Physiological Phenomena Laboratory Manuals Neurobiology methods Laboratory Manuals Neurons Laboratory manuals Neurons physiology Laboratory Manuals Neurotransmitter Agents physiology Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf Neurobiologie (DE-588)4041871-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Neurobiologie (DE-588)4041871-6 s Molekularbiologie (DE-588)4039983-7 s Labortechnik (DE-588)4123602-6 s DE-604 Cytologie (DE-588)4070177-3 s b DE-604 Gerfen, Charles R. Sonstige oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014815095&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience Cytology Laboratory manuals Gene Expression Regulation physiology Laboratory Manuals Molecular neurobiology Laboratory manuals Nervous System Physiological Phenomena Laboratory Manuals Neurobiology methods Laboratory Manuals Neurons Laboratory manuals Neurons physiology Laboratory Manuals Neurotransmitter Agents physiology Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Cytologie (DE-588)4070177-3 gnd Neurobiologie (DE-588)4041871-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4070177-3 (DE-588)4041871-6 (DE-588)4123602-6 |
| title | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |
| title_auth | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |
| title_exact_search | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |
| title_exact_search_txtP | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |
| title_full | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience ed. board Charles R. Gerfen ... |
| title_fullStr | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience ed. board Charles R. Gerfen ... |
| title_full_unstemmed | Short protocols in neuroscience cellular and molecular methods ; a compendium of methods from current protocols in neuroscience ed. board Charles R. Gerfen ... |
| title_short | Short protocols in neuroscience |
| title_sort | short protocols in neuroscience cellular and molecular methods a compendium of methods from current protocols in neuroscience |
| title_sub | cellular and molecular methods ; a compendium of methods from current protocols in neuroscience |
| topic | Cytology Laboratory manuals Gene Expression Regulation physiology Laboratory Manuals Molecular neurobiology Laboratory manuals Nervous System Physiological Phenomena Laboratory Manuals Neurobiology methods Laboratory Manuals Neurons Laboratory manuals Neurons physiology Laboratory Manuals Neurotransmitter Agents physiology Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Cytologie (DE-588)4070177-3 gnd Neurobiologie (DE-588)4041871-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Cytology Laboratory manuals Gene Expression Regulation physiology Laboratory Manuals Molecular neurobiology Laboratory manuals Nervous System Physiological Phenomena Laboratory Manuals Neurobiology methods Laboratory Manuals Neurons Laboratory manuals Neurons physiology Laboratory Manuals Neurotransmitter Agents physiology Laboratory Manuals Molekularbiologie Cytologie Neurobiologie Labortechnik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014815095&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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