Embryonic stem cell protocols: 1 Isolation and characterization
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Human Press
2006
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
329 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXI, 510 S. Ill., graph. Darst. |
| ISBN: | 9781588294982 1588294986 |
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| 245 | 1 | 0 | |a Embryonic stem cell protocols |n 1 |p Isolation and characterization |c ed. by Kursad Turksen |
| 250 | |a 2. ed. | ||
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Datensatz im Suchindex
| _version_ | 1804135290924695552 |
|---|---|
| adam_text | Contents
Preface v
Contents of the Companion Volume xi
Contributors xv
Guide to the Companion CD xxi
Part I. Isolation and Maintenance
1 Isolation and Differentiation of Medaka Embryonic Stem Cells
Yunhan Hong and Manfred Schartl 3
2 Maintenance of Chicken Embryonic Stem Cells In Vitro
Hiroyuki Horiuchi, Shuichi Furusawa,
and Haruo Matsuda 17
3 Derivation and Culture of Mouse Trophoblast
Stem Cells In Vitro
Satoshi Tanaka 35
4 Derivation, Maintenance, and Characterization
of Rat Embryonic Stem Cells In Vitro
Maren Schulze, Hendrik Ungefroren, Michael Bader,
and Fred Fandrich 45
5 Derivation, Maintenance, and Induction of the Differentiation
In Vitro of Equine Embryonic Stem Cells
Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai,
Takehide Murata, and Kazunari K. Yokoyama 59
6 Generation and Characterization of Monkey
Embryonic Stem Cells
Hirofumi Suemori and Norio Nakatsuji 81
7 Derivation and Propagation of Embryonic Stem Cells
in Serum and Feeder Free Culture
Jennifer Nichols and Qi Long Ying 91
Part II. Signaling in Embryonic Stem Cell Differentiation
8 Internal Standards in Differentiating
Embryonic Stem Cells In Vitro
Christopher L. Murphy 101
viii Contents
9 Matrix Assembly, Cell Polarization, and Cell Survival:
Analysis of Peri Implantation Development With Cultured
Embryonic Stem Cells
Shaohua Li and Peter D. Yurchenco 113
10 Phosphoinositides, Inositol Phosphates, and Phospholipase C
in Embryonic Stem Cells
Leo R. Quintan 127
11 Cripto Signaling in Differentiating Embryonic Stem Cells
Gabriella Minchiotti, Silvia Parisi,
and M. Graziella Persico 151
12 The Use of Embryonic Stem Cells to Study Hedgehog Signaling
Sandy Becker and Laura Grabel 171
1 3 Transfection and Promoter Analysis in Embryonic Stem Cells
Sangmi Chung and Kwang Soo Kim 187
14 SAGE Analysis to Identify Embryonic Stem Cell Predominant
Transcripts
Kenneth R. Boheler and Kirill V. Tarasov 195
15 Utilization of Digital Differential Display to Identify
Novel Targets of Oct3/4
Yoshimi Tokuzawa, Masayoshi Maruyama,
and Shinya Yamanaka 223
16 Gene Silencing Using RNA Interference in Embryonic Stem Cells
J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch,
and K. Sue O Shea 233
Part III. Genetic Manipulation of Embryonic Stem Cells
1 7 Efficient Transfer of HSV 1 Amplicon Vectors Into Embryonic
Stem Cells and Their Derivatives
Dieter Riethmacher, Filip Lim, and Thomas Schimmang 265
18 Lentiviral Vector Mediated Gene Transfer in Embryonic
Stem Cells
Masahiro Oka, Lung Ji Chang, Frank Costantini,
and Naohiro Terada 273
19 Use of the Cytomegalovirus Promoter
for Transient and Stable Transgene Expression
in Mouse Embryonic Stem Cells
Katie M. Barrow, Flor M. Perez Campo,
and Christopher M. Ward 283
Contents /x
20 Use of Simian Immunodeficiency Virus Vectors
for Simian Embryonic Stem Cells
Takayuki Asano, Hiroaki Shibata, and Yutaka Hanazono 295
21 Generation of Green Fluorescent Protein Expressing
Monkey Embryonic Stem Cells
Tatsuyuki Takada, Yutaka Suzuki, Mae Kadota,
Yasushi Kondo, and Ryuzo Torii 305
22 DNA Damage Response and Mutagenesis
in Mouse Embryonic Stem Cells
Yiling Hong, Rachel B. Cervantes, and Peter ). Stambrook 313
23 Ultraviolet Induced Apoptosis in Embryonic Stem Cells In Vitro
DakangXu, Trevor J. Wilson, and Paul J. Hertzog 327
Part IV. Use of Embryonic Stem Cells in Pharmacological
AND TOXICOLOGICAL SCREENS
24 Use of Differentiating Embryonic Stem Cells
in Pharmacological Studies
Brigitte Wdziekonski, Phi Villageois, Cecile Vernochet,
Blaine Phillips, and Christian Dani 341
25 Embryonic Stem Cells as a Source of Differentiated Neural
Cells for Pharmacological Screens
Patrick J. Mee, Carmel M. O Brien, Hazel Thomson,
Sjaak van der Sar, Viktor Lakics,
and Timothy E. Allsopp 353
26 Use of Murine Embryonic Stem Cells in Embryotoxicity Assays:
The Embryonic Stem Cell Test
Andrea E. M. Seiler, Roland Buesen, Anke Visan,
and Horst Spielmann 371
27 Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells
Sonja Becker, Martin Hrabe de Angelis,
and Johannes Beckers 397
Part V. Epigenetic Analysis of Embryonic Stem Cells
28 Nuclear Reprogramming of Somatic Nucleus Hybridized
With Embryonic Stem Cells by Electrofusion
Masako Tada and Takashi Tada 411
29 Methylation in Embryonic Stem Cells In Vitro
Koichiro Nishino, fun Ohgane, Masako Suzuki,
Naka Hattori, and Kunio Shiota 421
x Contents
Part VI. Tumor Like Properties
30 Identification of Genes Involved in Tumor Like Properties
of Embryonic Stem Cells
Kazutoshi Takahashi, Tomoko Ichisaka,
and Shinya Yamanaka 449
31 In Vivo Tumor Formation From Primate Embryonic Stem Cells
Takayuki Asano, Kyoko Sasaki, Yoshihiro Kitano,
Keiji Terao, and Yutaka Hanazono 459
Part VII. Animal Models and Therapy
32 Directed Differentiation and Characterization of Genetically
Modified Embryonic Stem Cells for Therapy
Adeline A. Lau, Kim M. Hemsley, Adrian Meedeniya,
Aaron J. Robinson, and John J. Hopwood 471
33 Use of Differentiating Embryonic Stem Cells
in the Parkinsonian Mouse Model
Fumihiko Nishimura, Hayato Toriumi, Shigeaki Ishizaka,
Toshisuke Sakaki, and Masahide Yoshikawa 485
Index 495
|
| adam_txt |
Contents
Preface v
Contents of the Companion Volume xi
Contributors xv
Guide to the Companion CD xxi
Part I. Isolation and Maintenance
1 Isolation and Differentiation of Medaka Embryonic Stem Cells
Yunhan Hong and Manfred Schartl 3
2 Maintenance of Chicken Embryonic Stem Cells In Vitro
Hiroyuki Horiuchi, Shuichi Furusawa,
and Haruo Matsuda 17
3 Derivation and Culture of Mouse Trophoblast
Stem Cells In Vitro
Satoshi Tanaka 35
4 Derivation, Maintenance, and Characterization
of Rat Embryonic Stem Cells In Vitro
Maren Schulze, Hendrik Ungefroren, Michael Bader,
and Fred Fandrich 45
5 Derivation, Maintenance, and Induction of the Differentiation
In Vitro of Equine Embryonic Stem Cells
Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai,
Takehide Murata, and Kazunari K. Yokoyama 59
6 Generation and Characterization of Monkey
Embryonic Stem Cells
Hirofumi Suemori and Norio Nakatsuji 81
7 Derivation and Propagation of Embryonic Stem Cells
in Serum and Feeder Free Culture
Jennifer Nichols and Qi Long Ying 91
Part II. Signaling in Embryonic Stem Cell Differentiation
8 Internal Standards in Differentiating
Embryonic Stem Cells In Vitro
Christopher L. Murphy 101
viii Contents
9 Matrix Assembly, Cell Polarization, and Cell Survival:
Analysis of Peri Implantation Development With Cultured
Embryonic Stem Cells
Shaohua Li and Peter D. Yurchenco 113
10 Phosphoinositides, Inositol Phosphates, and Phospholipase C
in Embryonic Stem Cells
Leo R. Quintan 127
11 Cripto Signaling in Differentiating Embryonic Stem Cells
Gabriella Minchiotti, Silvia Parisi,
and M. Graziella Persico 151
12 The Use of Embryonic Stem Cells to Study Hedgehog Signaling
Sandy Becker and Laura Grabel 171
1 3 Transfection and Promoter Analysis in Embryonic Stem Cells
Sangmi Chung and Kwang Soo Kim 187
14 SAGE Analysis to Identify Embryonic Stem Cell Predominant
Transcripts
Kenneth R. Boheler and Kirill V. Tarasov 195
15 Utilization of Digital Differential Display to Identify
Novel Targets of Oct3/4
Yoshimi Tokuzawa, Masayoshi Maruyama,
and Shinya Yamanaka 223
16 Gene Silencing Using RNA Interference in Embryonic Stem Cells
J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch,
and K. Sue O'Shea 233
Part III. Genetic Manipulation of Embryonic Stem Cells
1 7 Efficient Transfer of HSV 1 Amplicon Vectors Into Embryonic
Stem Cells and Their Derivatives
Dieter Riethmacher, Filip Lim, and Thomas Schimmang 265
18 Lentiviral Vector Mediated Gene Transfer in Embryonic
Stem Cells
Masahiro Oka, Lung Ji Chang, Frank Costantini,
and Naohiro Terada 273
19 Use of the Cytomegalovirus Promoter
for Transient and Stable Transgene Expression
in Mouse Embryonic Stem Cells
Katie M. Barrow, Flor M. Perez Campo,
and Christopher M. Ward 283
Contents /x
20 Use of Simian Immunodeficiency Virus Vectors
for Simian Embryonic Stem Cells
Takayuki Asano, Hiroaki Shibata, and Yutaka Hanazono 295
21 Generation of Green Fluorescent Protein Expressing
Monkey Embryonic Stem Cells
Tatsuyuki Takada, Yutaka Suzuki, Mae Kadota,
Yasushi Kondo, and Ryuzo Torii 305
22 DNA Damage Response and Mutagenesis
in Mouse Embryonic Stem Cells
Yiling Hong, Rachel B. Cervantes, and Peter ). Stambrook 313
23 Ultraviolet Induced Apoptosis in Embryonic Stem Cells In Vitro
DakangXu, Trevor J. Wilson, and Paul J. Hertzog 327
Part IV. Use of Embryonic Stem Cells in Pharmacological
AND TOXICOLOGICAL SCREENS
24 Use of Differentiating Embryonic Stem Cells
in Pharmacological Studies
Brigitte Wdziekonski, Phi Villageois, Cecile Vernochet,
Blaine Phillips, and Christian Dani 341
25 Embryonic Stem Cells as a Source of Differentiated Neural
Cells for Pharmacological Screens
Patrick J. Mee, Carmel M. O'Brien, Hazel Thomson,
Sjaak van der Sar, Viktor Lakics,
and Timothy E. Allsopp 353
26 Use of Murine Embryonic Stem Cells in Embryotoxicity Assays:
The Embryonic Stem Cell Test
Andrea E. M. Seiler, Roland Buesen, Anke Visan,
and Horst Spielmann 371
27 Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells
Sonja Becker, Martin Hrabe de Angelis,
and Johannes Beckers 397
Part V. Epigenetic Analysis of Embryonic Stem Cells
28 Nuclear Reprogramming of Somatic Nucleus Hybridized
With Embryonic Stem Cells by Electrofusion
Masako Tada and Takashi Tada 411
29 Methylation in Embryonic Stem Cells In Vitro
Koichiro Nishino, fun Ohgane, Masako Suzuki,
Naka Hattori, and Kunio Shiota 421
x Contents
Part VI. Tumor Like Properties
30 Identification of Genes Involved in Tumor Like Properties
of Embryonic Stem Cells
Kazutoshi Takahashi, Tomoko Ichisaka,
and Shinya Yamanaka 449
31 In Vivo Tumor Formation From Primate Embryonic Stem Cells
Takayuki Asano, Kyoko Sasaki, Yoshihiro Kitano,
Keiji Terao, and Yutaka Hanazono 459
Part VII. Animal Models and Therapy
32 Directed Differentiation and Characterization of Genetically
Modified Embryonic Stem Cells for Therapy
Adeline A. Lau, Kim M. Hemsley, Adrian Meedeniya,
Aaron J. Robinson, and John J. Hopwood 471
33 Use of Differentiating Embryonic Stem Cells
in the Parkinsonian Mouse Model
Fumihiko Nishimura, Hayato Toriumi, Shigeaki Ishizaka,
Toshisuke Sakaki, and Masahide Yoshikawa 485
Index 495 |
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| spelling | Embryonic stem cell protocols 1 Isolation and characterization ed. by Kursad Turksen 2. ed. Totowa, NJ Human Press 2006 XXI, 510 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 329 Methods in molecular biology ... Turksen, Kursad Sonstige oth (DE-604)BV021540094 1 Methods in molecular biology 329 (DE-604)BV035362695 329 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014756322&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Embryonic stem cell protocols Methods in molecular biology |
| title | Embryonic stem cell protocols |
| title_auth | Embryonic stem cell protocols |
| title_exact_search | Embryonic stem cell protocols |
| title_exact_search_txtP | Embryonic stem cell protocols |
| title_full | Embryonic stem cell protocols 1 Isolation and characterization ed. by Kursad Turksen |
| title_fullStr | Embryonic stem cell protocols 1 Isolation and characterization ed. by Kursad Turksen |
| title_full_unstemmed | Embryonic stem cell protocols 1 Isolation and characterization ed. by Kursad Turksen |
| title_short | Embryonic stem cell protocols |
| title_sort | embryonic stem cell protocols isolation and characterization |
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