Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate: their effects on renal mesangial cells under inflammatory conditions
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| Format: | Book |
| Language: | English German |
| Published: |
2005
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Frankfurt (Main), Univ., Diss., 2005 |
| Physical Description: | 133 S. graph. Darst. |
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| 100 | 1 | |a Xin, Cuiyan |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate |b their effects on renal mesangial cells under inflammatory conditions |c vorgelegt von Cuiyan Xin |
| 264 | 1 | |c 2005 | |
| 300 | |a 133 S. |b graph. Darst. | ||
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| 500 | |a Frankfurt (Main), Univ., Diss., 2005 | ||
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| adam_text | CONTENTS
CONTENTS
1 INTRODUCTION 1
1.1 The mesangial cells 1
1.1.1 Mesangial cells (MC) in the glomerulus 1
..1,2 Mesangial cells proliferation and glomerular diseases ?__
1.2 G protein-coupled receptors (GPCRs) and the signal diversity 2
1.3 Angiotensin II 5_ ..
1.3.1 The formation of angiotensin II and its receptors §__
1.3.2 The action and signaling of angiotensin II in mesangial cells 6__
1.4 Extracellular nucleotides 7_ ..
1.5 Sphingosine 1-phosphate
1.5.1 The generation and degradation of S1P 8
1.5.2 The S1P receptors and receptor-mediated signalling 9
1.5.3 The S1P analogue: FTY720 . 10
1.6 Aim of the study _U_
2 MATERIALS AND METHODS 13-
2.1 Materials 11-
2.1.1 Chemicals 15-
2.1.2 Antibodies and antisera 1S-
2.1.3 Enzymes 15-
2.1.4 Plasmids . 1£-
2.1.5 Eukaryotic cell lines 16-
2.1.6 Bacterial strains __!§-
2.1.7 Buffers . 1*L
2.1.8 Others 16-
2.2 Cell culture 16-
2.2.1 Culture and stimulation of mesangial cells ^ -
2.2.2 Culture and transfection of HEK 293 cells 17~
2.2.3 Gene silencing by sequence-specific siRNA reagents 17—
2.3 Bacterial culture 1*L
2.3.1 Competent bacteria 1§-
2.3.2 Transformation 12-
2.4 Nucleic acid techniques ^9 -
2.4.1 Preparation of plasmid DNA ^_
2.4.2 RNA isolation from cultured cells li-
2.4.3 Quantification of nucleic acids iQ-
2.4.4 Agarose gel electrophoresis . ?2_
2.4.5 DNA isolation from agarose gels ^ -
2.4.6 Reverse transcriptase polymerase chain reaction (RT-PCR) ^1 _
2.4.7 Manipulation of DNA __??--
2.4.7.1 Restriction ___??-
2.4.7.2 Ligation __-??-
2.4.8 DNA sequencing ?2—
2.4.9 Northern blot analysis -?2_
2.5 Protein Techniques 23-
2.5.1 Preparation of lysates ! —
2.5.2 Quantification of proteins ?i-
2.5.3 Protein precipitation by the trichloroacetic acid (TCA) method 24
2.5.4 Immunoprecipitation of proteins ?4—
CONTENTS
2.5.5 Metabolic labeling of proteins 25
2.5.6 Western blot analysis 25
2.5.7 In vivo phosphorylation 25
2.5.8 Cross-linking reaction 26
2.6 Measurement of cell parameters 26
2.6.1 sPLA; activity assay 26
2.6.2 Nitrite formation 27
2.6.3 Determination of inositol phosphate generation 27
2.6.4 Transforming growth factor-p (TGF-p) enzyme immunoassay 27
2.7 Statistical analysis 27
3 RESULTS 28_
3.1 lnterleukin-1 inhibits angiotensin ll-stimulated protein kinase B pathway in 28
renal mesangial cells via the induciable nitric oxide synthase
3.2 Interleukin-ip inhibits ATP-induced protein kinase B activation in renal 37
mesangial cells by two different mechanisms: the involvement of nitric
oxide and ceramide
3.3 Sphingosine 1-phosphate cross-activates the Smad signalling cascade and 45
mimics transforming growth factor-p-induced cell responses
3.4 Heterologous desensitization of the sphingosine-1 -phosphate receptors by 59
purinoceptor activation in renal mesangial cells
3.5 The immunosuppressive agent FTY720 and its phosphorylated derivative 70
activate the Smad signalling cascade and upregulate connective tissue
growth factor (CTGF) and collagen type IV expression in renal mesangial
cells
4 SUMMARIZING DISCUSSION 82
5 SUMMARY 86_
6 REFERENCE 88_
7 SUPPLEMENT 126_
7.1 Abbreviations 126
7.2 Publications 128
7.3 Acknowledgement 130
7.4 Deutsche Zusammenfassung 131
7.5 Curriculum vitae 133
|
| adam_txt |
CONTENTS
CONTENTS
1 INTRODUCTION 1
1.1 The mesangial cells 1
1.1.1 Mesangial cells (MC) in the glomerulus 1
.1,2 Mesangial cells proliferation and glomerular diseases ?_
1.2 G protein-coupled receptors (GPCRs) and the signal diversity 2
1.3 Angiotensin II 5_ .
1.3.1 The formation of angiotensin II and its receptors §_
1.3.2 The action and signaling of angiotensin II in mesangial cells 6_
1.4 Extracellular nucleotides 7_ .
1.5 Sphingosine 1-phosphate '
1.5.1 The generation and degradation of S1P 8
1.5.2 The S1P receptors and receptor-mediated signalling 9
1.5.3 The S1P analogue: FTY720 . 10
1.6 Aim of the study _U_
2 MATERIALS AND METHODS 13-
2.1 Materials 11-
2.1.1 Chemicals 15-
2.1.2 Antibodies and antisera 1S-
2.1.3 Enzymes 15-
2.1.4 Plasmids . 1£-
2.1.5 Eukaryotic cell lines 16-
2.1.6 Bacterial strains _!§-
2.1.7 Buffers . 1*L
2.1.8 Others 16-
2.2 Cell culture 16-
2.2.1 Culture and stimulation of mesangial cells ^ -
2.2.2 Culture and transfection of HEK 293 cells 17~
2.2.3 Gene silencing by sequence-specific siRNA reagents 17—
2.3 Bacterial culture 1*L
2.3.1 Competent bacteria 1§-
2.3.2 Transformation 12-
2.4 Nucleic acid techniques ^9 -
2.4.1 Preparation of plasmid DNA ^_
2.4.2 RNA isolation from cultured cells li-
2.4.3 Quantification of nucleic acids iQ-
2.4.4 Agarose gel electrophoresis . ?2_
2.4.5 DNA isolation from agarose gels ^ -
2.4.6 Reverse transcriptase polymerase chain reaction (RT-PCR) ^1 _
2.4.7 Manipulation of DNA _??--
2.4.7.1 Restriction _??-
2.4.7.2 Ligation _-??-
2.4.8 DNA sequencing ?2—
2.4.9 Northern blot analysis -?2_
2.5 Protein Techniques 23-
2.5.1 Preparation of lysates '! —
2.5.2 Quantification of proteins ?i-
2.5.3 Protein precipitation by the trichloroacetic acid (TCA) method 24
2.5.4 Immunoprecipitation of proteins ?4—
CONTENTS
2.5.5 Metabolic labeling of proteins 25
2.5.6 Western blot analysis 25
2.5.7 In vivo phosphorylation 25
2.5.8 Cross-linking reaction 26
2.6 Measurement of cell parameters 26
2.6.1 sPLA; activity assay 26
2.6.2 Nitrite formation 27
2.6.3 Determination of inositol phosphate generation 27
2.6.4 Transforming growth factor-p (TGF-p) enzyme immunoassay 27
2.7 Statistical analysis 27
3 RESULTS 28_
3.1 lnterleukin-1 inhibits angiotensin ll-stimulated protein kinase B pathway in 28
renal mesangial cells via the induciable nitric oxide synthase
3.2 Interleukin-ip inhibits ATP-induced protein kinase B activation in renal 37
mesangial cells by two different mechanisms: the involvement of nitric
oxide and ceramide
3.3 Sphingosine 1-phosphate cross-activates the Smad signalling cascade and 45
mimics transforming growth factor-p-induced cell responses
3.4 Heterologous desensitization of the sphingosine-1 -phosphate receptors by 59
purinoceptor activation in renal mesangial cells
3.5 The immunosuppressive agent FTY720 and its phosphorylated derivative 70
activate the Smad signalling cascade and upregulate connective tissue
growth factor (CTGF) and collagen type IV expression in renal mesangial
cells
4 SUMMARIZING DISCUSSION 82
5 SUMMARY 86_
6 REFERENCE 88_
7 SUPPLEMENT 126_
7.1 Abbreviations 126
7.2 Publications 128
7.3 Acknowledgement 130
7.4 Deutsche Zusammenfassung 131
7.5 Curriculum vitae 133 |
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| index_date | 2024-07-02T14:19:55Z |
| indexdate | 2024-07-09T20:37:33Z |
| institution | BVB |
| language | English German |
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| physical | 133 S. graph. Darst. |
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| spelling | Xin, Cuiyan Verfasser aut Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions vorgelegt von Cuiyan Xin 2005 133 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Frankfurt (Main), Univ., Diss., 2005 Zsfassung in dt. Sprache (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014730839&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Xin, Cuiyan Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions |
| subject_GND | (DE-588)4113937-9 |
| title | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions |
| title_auth | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions |
| title_exact_search | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions |
| title_exact_search_txtP | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions |
| title_full | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions vorgelegt von Cuiyan Xin |
| title_fullStr | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions vorgelegt von Cuiyan Xin |
| title_full_unstemmed | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate their effects on renal mesangial cells under inflammatory conditions vorgelegt von Cuiyan Xin |
| title_short | Angiotensin II, extracellular nucleotides and sphingosine-1-phosphate |
| title_sort | angiotensin ii extracellular nucleotides and sphingosine 1 phosphate their effects on renal mesangial cells under inflammatory conditions |
| title_sub | their effects on renal mesangial cells under inflammatory conditions |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014730839&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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