Analytical ultracentrifugation of polymers and nanoparticles:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin [u.a.]
Springer
2006
|
| Series: | Springer Laboratory
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 237 S. Ill., graph. Darst. 235 mm x 155 mm |
| ISBN: | 3540234322 9783540234326 |
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| 245 | 1 | 0 | |a Analytical ultracentrifugation of polymers and nanoparticles |c Walter Mächtle ; Lars Börger |
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Record in the Search Index
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| adam_text | Table of Contents
1 INTRODUCTION........................................................ 1
1.1 Historic Examples of Ultracentrifugation...................... 2
1.1.1 Investigations on Gold Colloids in 1924 ............... 3
1.1.2 Investigations on the Structure of DNA in 1957 ........ 5
1.2 Basic Theory of Ultracentrifugation........................... 7
1.2.1 Svedberg’s Simplified Theory........................... 8
1.2.2 Derivation of Lamm’s Equation......................... 10
1.3 Basic Experiment Types of Ultracentrifugation................ 12
1.3.1 Sedimentation Velocity Experiment..................... 12
1.3.2 Synthetic Boundary Experiment......................... 13
1.3.3 Sedimentation Equilibrium............................. 13
1.3.4 Density Gradient...................................... 14
1.3.5 Approach-to-Equilibrium (or Archibald) Method....... 15
1.4 Closing Remarks.............................................. 15
References........................................................ 16
2 ANALYTICAL ULTRACENTRIFUGATION, INSTRUMENTATION.................. 17
2.1 Ultracentrifuges............................................. 18
2.1.1 The Beckman-Coulter Optima XL-A/I .................... 19
2.1.2 User-Made Centrifuges ................................ 21
2.2 Rotors ...................................................... 25
2.3 Measuring Cells.............................................. 26
2.4 Detectors.................................................... 29
2.4.1 Absorption Optics..................................... 31
2.4.2 Interference Optics................................... 34
2.4.3 Schlieren Optics ..................................... 37
2.4.4 Other Detectors....................................... 38
2.5 Multiplexer.................................................. 43
2.6 Auxiliary Measurements....................................... 43
2.6.1 Measurement of the Solvent Density
and the Partial Specific Volume....................... 43
2.6.2 Measurement of the Refractive Index
and the Specific Refractive Index Increment........... 45
References........................................................ 46
XII
Table of Contents
3 SEDIMENTATION VELOCITY................................................ 47
3.1 Introduction.................................................... 47
3.2 Basic Example of Sedimentation Velocity......................... 47
3.2.1 Determination of s....................................... 50
3.2.2 Standard Conditions for s Estimation..................... 51
3.2.3 Radial Dilution and Thickening........................... 52
3.2.4 Concentration Dependence................................. 53
3.3 Advanced Theory of Sedimentation Velocity Runs.................. 54
3.3.1 Johnston-Ogston Effect................................... 54
3.3.2 Self-Sharpening of Boundaries............................ 55
3.3.3 Pressure Dependence...................................... 55
3.3.4 Speed Dependence......................................... 56
3.3.5 Charge Effects........................................... 57
3.3.6 Separation of Sedimentation and Diffusion................ 58
3.3.7 Test of Homogeneity...................................... 61
3.4 Sedimentation Velocity Rims of Macromolecules
to Measure Average M and MMD.............................. 62
3.4.1 Evaluation of the Average Molar Mass M
by Sedimentation Velocity Rims via Scaling Laws.......... 62
3.4.2 Evaluation of Molar Mass Distributions (MMD)
by Sedimentation Velocity Runs via Scaling Laws.......... 64
3.5 Sedimentation Velocity Runs on Particles
to Measure Average dv and PSD................................... 69
3.5.1 Particle Size Distribution via AUC Tbrbidity Detector
and Mie Theory........................................... 70
3.5.2 Coupling Technique to Measure very Broad PSD ............ 77
3.5.3 H20-D20 Density Variation Method to Measure Particle
Densities via Sedimentation Velocity Runs ............... 80
3.5.4 PSD Measurement of very Small Platinum Clusters
Using UV Optics.......................................... 84
3.6 Synthetic Boundary Experiments.................................. 86
3.6.1 Synthetic Boundary Crystallization Ultracentrifugation .. 93
References........................................................... 95
4 DENSITY GRADIENTS .................................................... 97
4.1 Introduction.................................................... 98
4.2 Static Density Gradients....................................... 101
4.2.1 Theory of Static Density Gradients...................... 102
4.2.2 Gradient Materials ..................................... 115
4.2.3 Experimental Procedure ................................. 117
4.2.4 Examples ............................................... 118
4.3 Dynamic Density Gradients...................................... 120
4.4 Other Types of Density Gradients............................... 125
4.4.1 Percoll Density Gradient................................ 125
References.......................................................... 129
Table of Contents
XIII
5 SEDIMENTATION EQUILIBRIUM ....................................... 131
5.1 Introduction............................................... 131
5.2 Experimental Procedure..................................... 133
5.3 Data Analysis.............................................. 138
5.3.1 Basic Equations for Molar Mass Averages -
Classical Evaluation of AUC Equilibrium Runs........ 138
5.3.2 Basic Equations for Molar Mass Distributions -
Nonlinear Regression Evaluation (Lechner Method).... 141
5.3.3 Application of the Basic Equations -
Classical and Nonlinear Regression Evaluation....... 144
5.3.4 M-STAR, a Special Data Analysis to obtain Mw........ 149
5.4 Examples................................................... 151
5.4.1 Absorption Optics Examples of a Polyelectrolyte
and Calibration Polystyrene NBS 706 ................ 152
5.4.2 Association Equilibrium Example
and AUC Buoyant Density Method to Determine v ...... 155
5.4.3 The New Fluorescence Detector of Laue Used
for Green Fluorescent Protein....................... 158
5.5 Further AUC Methods to Measure Molar Masses................ 159
References...................................................... 162
6 PRACTICAL EXAMPLES OF COMBINATION OF METHODS..................... 163
6.1 Combination of Different AUC Methods....................... 163
6.1.1 Core/Shell Particles................................ 163
6.1.2 Characterization of Microgels and Nanogels.......... 170
6.1.3 Ion Exchange in Carboxylated Latices................ 179
6.2 Combination of AUC with Other Techniques................... 189
6.2.1 AUC and SFFF........................................ 190
6.2.2 AUC and EM ......................................... 198
6.3 Literature Examples: AUC and Nanoparticles ................ 212
References...................................................... 212
7 RECENT DEVELOPMENTS AND FUTURE OUTLOOK........................... 215
7.1 New AUC Instrumentation and Detectors...................... 216
7.2 New AUC Methods ........................................... 222
7.3 New AUC Data Analysis...................................... 228
References...................................................... 233
8 SUBJECT INDEX
235
|
| adam_txt |
Table of Contents
1 INTRODUCTION. 1
1.1 Historic Examples of Ultracentrifugation. 2
1.1.1 Investigations on Gold Colloids in 1924 . 3
1.1.2 Investigations on the Structure of DNA in 1957 . 5
1.2 Basic Theory of Ultracentrifugation. 7
1.2.1 Svedberg’s Simplified Theory. 8
1.2.2 Derivation of Lamm’s Equation. 10
1.3 Basic Experiment Types of Ultracentrifugation. 12
1.3.1 Sedimentation Velocity Experiment. 12
1.3.2 Synthetic Boundary Experiment. 13
1.3.3 Sedimentation Equilibrium. 13
1.3.4 Density Gradient. 14
1.3.5 Approach-to-Equilibrium (or Archibald) Method. 15
1.4 Closing Remarks. 15
References. 16
2 ANALYTICAL ULTRACENTRIFUGATION, INSTRUMENTATION. 17
2.1 Ultracentrifuges. 18
2.1.1 The Beckman-Coulter Optima XL-A/I . 19
2.1.2 User-Made Centrifuges . 21
2.2 Rotors . 25
2.3 Measuring Cells. 26
2.4 Detectors. 29
2.4.1 Absorption Optics. 31
2.4.2 Interference Optics. 34
2.4.3 Schlieren Optics . 37
2.4.4 Other Detectors. 38
2.5 Multiplexer. 43
2.6 Auxiliary Measurements. 43
2.6.1 Measurement of the Solvent Density
and the Partial Specific Volume. 43
2.6.2 Measurement of the Refractive Index
and the Specific Refractive Index Increment. 45
References. 46
XII
Table of Contents
3 SEDIMENTATION VELOCITY. 47
3.1 Introduction. 47
3.2 Basic Example of Sedimentation Velocity. 47
3.2.1 Determination of s. 50
3.2.2 Standard Conditions for s Estimation. 51
3.2.3 Radial Dilution and Thickening. 52
3.2.4 Concentration Dependence. 53
3.3 Advanced Theory of Sedimentation Velocity Runs. 54
3.3.1 Johnston-Ogston Effect. 54
3.3.2 Self-Sharpening of Boundaries. 55
3.3.3 Pressure Dependence. 55
3.3.4 Speed Dependence. 56
3.3.5 Charge Effects. 57
3.3.6 Separation of Sedimentation and Diffusion. 58
3.3.7 Test of Homogeneity. 61
3.4 Sedimentation Velocity Rims of Macromolecules
to Measure Average M and MMD. 62
3.4.1 Evaluation of the Average Molar Mass M
by Sedimentation Velocity Rims via Scaling Laws. 62
3.4.2 Evaluation of Molar Mass Distributions (MMD)
by Sedimentation Velocity Runs via Scaling Laws. 64
3.5 Sedimentation Velocity Runs on Particles
to Measure Average dv and PSD. 69
3.5.1 Particle Size Distribution via AUC Tbrbidity Detector
and Mie Theory. 70
3.5.2 Coupling Technique to Measure very Broad PSD . 77
3.5.3 H20-D20 Density Variation Method to Measure Particle
Densities via Sedimentation Velocity Runs . 80
3.5.4 PSD Measurement of very Small Platinum Clusters
Using UV Optics. 84
3.6 Synthetic Boundary Experiments. 86
3.6.1 Synthetic Boundary Crystallization Ultracentrifugation . 93
References. 95
4 DENSITY GRADIENTS . 97
4.1 Introduction. 98
4.2 Static Density Gradients. 101
4.2.1 Theory of Static Density Gradients. 102
4.2.2 Gradient Materials . 115
4.2.3 Experimental Procedure . 117
4.2.4 Examples . 118
4.3 Dynamic Density Gradients. 120
4.4 Other Types of Density Gradients. 125
4.4.1 Percoll Density Gradient. 125
References. 129
Table of Contents
XIII
5 SEDIMENTATION EQUILIBRIUM . 131
5.1 Introduction. 131
5.2 Experimental Procedure. 133
5.3 Data Analysis. 138
5.3.1 Basic Equations for Molar Mass Averages -
Classical Evaluation of AUC Equilibrium Runs. 138
5.3.2 Basic Equations for Molar Mass Distributions -
Nonlinear Regression Evaluation (Lechner Method). 141
5.3.3 Application of the Basic Equations -
Classical and Nonlinear Regression Evaluation. 144
5.3.4 M-STAR, a Special Data Analysis to obtain Mw. 149
5.4 Examples. 151
5.4.1 Absorption Optics Examples of a Polyelectrolyte
and Calibration Polystyrene NBS 706 . 152
5.4.2 Association Equilibrium Example
and AUC Buoyant Density Method to Determine v . 155
5.4.3 The New Fluorescence Detector of Laue Used
for Green Fluorescent Protein. 158
5.5 Further AUC Methods to Measure Molar Masses. 159
References. 162
6 PRACTICAL EXAMPLES OF COMBINATION OF METHODS. 163
6.1 Combination of Different AUC Methods. 163
6.1.1 Core/Shell Particles. 163
6.1.2 Characterization of Microgels and Nanogels. 170
6.1.3 Ion Exchange in Carboxylated Latices. 179
6.2 Combination of AUC with Other Techniques. 189
6.2.1 AUC and SFFF. 190
6.2.2 AUC and EM . 198
6.3 Literature Examples: AUC and Nanoparticles . 212
References. 212
7 RECENT DEVELOPMENTS AND FUTURE OUTLOOK. 215
7.1 New AUC Instrumentation and Detectors. 216
7.2 New AUC Methods . 222
7.3 New AUC Data Analysis. 228
References. 233
8 SUBJECT INDEX
235 |
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| index_date | 2024-07-02T14:00:40Z |
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| record_format | marc |
| series2 | Springer Laboratory |
| spelling | Mächtle, Walter Verfasser aut Analytical ultracentrifugation of polymers and nanoparticles Walter Mächtle ; Lars Börger Berlin [u.a.] Springer 2006 XIII, 237 S. Ill., graph. Darst. 235 mm x 155 mm txt rdacontent n rdamedia nc rdacarrier Springer Laboratory Bioquímica larpcal Nanotecnologia larpcal Polímeros (química orgânica) larpcal Química analítica instrumental larpcal Nanoparticles Analysis Nanostructures analysis Polymers Analysis Polymers analysis Ultracentrifugation Ultracentrifugation methods Ultrazentrifugieren (DE-588)4186746-4 gnd rswk-swf Analytische Chemie (DE-588)4129906-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Analytische Chemie (DE-588)4129906-1 s Ultrazentrifugieren (DE-588)4186746-4 s DE-604 Börger, Lars Verfasser aut KOBV Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014647556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mächtle, Walter Börger, Lars Analytical ultracentrifugation of polymers and nanoparticles Bioquímica larpcal Nanotecnologia larpcal Polímeros (química orgânica) larpcal Química analítica instrumental larpcal Nanoparticles Analysis Nanostructures analysis Polymers Analysis Polymers analysis Ultracentrifugation Ultracentrifugation methods Ultrazentrifugieren (DE-588)4186746-4 gnd Analytische Chemie (DE-588)4129906-1 gnd |
| subject_GND | (DE-588)4186746-4 (DE-588)4129906-1 (DE-588)4143413-4 |
| title | Analytical ultracentrifugation of polymers and nanoparticles |
| title_auth | Analytical ultracentrifugation of polymers and nanoparticles |
| title_exact_search | Analytical ultracentrifugation of polymers and nanoparticles |
| title_exact_search_txtP | Analytical ultracentrifugation of polymers and nanoparticles |
| title_full | Analytical ultracentrifugation of polymers and nanoparticles Walter Mächtle ; Lars Börger |
| title_fullStr | Analytical ultracentrifugation of polymers and nanoparticles Walter Mächtle ; Lars Börger |
| title_full_unstemmed | Analytical ultracentrifugation of polymers and nanoparticles Walter Mächtle ; Lars Börger |
| title_short | Analytical ultracentrifugation of polymers and nanoparticles |
| title_sort | analytical ultracentrifugation of polymers and nanoparticles |
| topic | Bioquímica larpcal Nanotecnologia larpcal Polímeros (química orgânica) larpcal Química analítica instrumental larpcal Nanoparticles Analysis Nanostructures analysis Polymers Analysis Polymers analysis Ultracentrifugation Ultracentrifugation methods Ultrazentrifugieren (DE-588)4186746-4 gnd Analytische Chemie (DE-588)4129906-1 gnd |
| topic_facet | Bioquímica Nanotecnologia Polímeros (química orgânica) Química analítica instrumental Nanoparticles Analysis Nanostructures analysis Polymers Analysis Polymers analysis Ultracentrifugation Ultracentrifugation methods Ultrazentrifugieren Analytische Chemie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014647556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT machtlewalter analyticalultracentrifugationofpolymersandnanoparticles AT borgerlars analyticalultracentrifugationofpolymersandnanoparticles |