Molecular microbial ecology:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York, NY [u.a.]
Taylor & Francis
2005
|
| Series: | BIOS advanced methods
|
| Subjects: | |
| Online Access: | Table of contents Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XVI, 381 S. graph. Darst. cm |
| ISBN: | 1859962831 |
Staff View
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| 245 | 1 | 0 | |a Molecular microbial ecology |c A. Mark Osborn and Cindy Smith (eds) |
| 264 | 1 | |a New York, NY [u.a.] |b Taylor & Francis |c 2005 | |
| 300 | |a XVI, 381 S. |b graph. Darst. |c cm | ||
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| 490 | 0 | |a BIOS advanced methods | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Microbiologie moléculaire | |
| 650 | 4 | |a Écologie microbienne | |
| 650 | 4 | |a Ökologie | |
| 650 | 4 | |a Molecular microbiology | |
| 650 | 4 | |a Microbial ecology | |
| 650 | 4 | |a Ecology | |
| 650 | 4 | |a Environmental Microbiology | |
| 650 | 4 | |a Nucleic Acid Hybridization | |
| 650 | 4 | |a Polymerase Chain Reaction |x methods | |
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| 700 | 1 | |a Smith, Cindy J. |e Sonstige |4 oth | |
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Record in the Search Index
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|---|---|
| adam_text | Molecular Microbial
Ecology
Dr A Mark Osborn (Ed )
Department of Animal amp; Plant Sciences, University of Sheffield,
Sheffield, UK
Dr Cindy J Smith (Ed )
Department of Biological Sciences, University of Essex,
Colchester, UK
Taylor amp; Francis
Taylor amp; Francis Group
Contents
Contributors xi
Abbreviations xii
Preface xiii
1 Nucleic acid extraction from environmental samples 1
Annett Milling, Newton C M Gomes, Miruna Oros-Sichler, Monika Gb tz and
Kornelia Smalla
1 1 Introduction 1
1 2 Recovery of cells from environmental matrices 2
1 3 Cell lysis and DNA extraction protocols 3
1 4 Immunochemical isolation of DNA from metabolically active cells 5
1 5 RNA extraction from environmental matrices 6
Protocol 1 1 11
Protocol 1 2 17
Protocol 13_ 21
Prokaryotic systematic^: PCR and sequence analysis of amplified 25
16S rRNA genes
Wilfred F M Roling and Ian M Head
2 1 Introduction - 25
2 2 Application of PCR in microbial ecology 26
221 Good working practices in PCR: avoiding PCR inhibition and 28
contamination
222 Pitfalls of PCR: artifacts and differential amplification 30
223 rRNA versus rDNA: indicative of active versus total microbial 32
communities?
2 3 Generation and analysis of clone libraries 34
231 Generation of clone libraries 34
232 Screening of clone libraries 35
2 4 Quantitative analysis of clone libraries: coverage and diversity indices 38
241 Coverage and sampling 38
242 Diversity indices 40
2 5 Phylogenetic inference 41
251 Sequencing 41
252 Phylogenetic analysis 42
253 Parsimony 44
254 Distance methods 46
vi Contents
255 Maximum likelihood 47
256 Bootstrap analysis 50
,26 Phylogenetic analysis compared to other molecular techniques 50
^261 Comparing large numbers of samples 51
262 Quantification 51
263 Relationship between phylogenetic information and function 52
2 7 Concluding remarks 52
Protocol 2 1 57
3 DNA fingerprinting of microbial communities 65
Andreas Felske and A Mark Osborn
3 1 Introduction 65
311 DGGE 66
312 TGGE 68
313 SSCP analysis 68
314 T-RFLP analysis 69
315 LH-PCR (length heterogeneity PCR) 70
316 Comparison of the different methods 71
3 2 Applications of microbial community fingerprinting 74
321 Combination of cloning with fingerprinting 76
322 Screening isolated strains next to fingerprints 76
323 Southern blot hybridization and fingerprints 77
324 Multiple competitor RT-PCR/TGGE for rRNA 78
325 Environmental fingerprints with protein-coding genes 79
3 3 Analyzing microbial community fingerprints 79
331 Cutting out fingerprint bands for sequencing 80
332 The rRNA sequence hetejogeneity 81
333 Quantitative analysis of fingerprint signals 82
334 Cluster analysis of DNA fingerprint signals 84
335 Diversity and species richness 84
336 The operational taxonomic unit 85
3 4 Miscellaneous-advice 85
Protocol 3 1 91
4 Molecular typing of environmental isolates 97
Jan L W Rademaker, Henk J M Aarts and Pablo Vinuesa
4 1 Introduction 97
411 Role of PCR amplification-based typing for microbial ecology 98
4 2 Microbial typing methods 98
421 AFLP analysis 99
422 rep-PCR fingerprinting 103
423 Mini- and microsatellite fingerprinting 106
424 Fingerprinting methods using random primers 106
(RAPD, AP-PCR, DAF)
425 Ribosomal RNA gene fingerprinting methods 108
4 3 Computer-assisted analysis of PCR-generated fingerprint profiles 113
4 4 Concluding remarks 114
Protocol 4 1 127
Protocol 4 2 131
Contents vii
RT-PCR and mRNA expression analysis of functional genes 135
Balbina Nogales
P5 1 Introduction 135
-^ 5 2 Advantages and limitations of the RT-PCR analysis of bacterial 136
functional genes
5 3 The RT-PCR reaction 137
531 The RNA template 137
532 Primers for reverse transcription 138
533 Reverse transcriptases 138
534 One-step and two-step RT-PCR protocols 139
5 4 Quantitative RT-PCR 140
5 5 Analysis of global gene expression 140
5 6 Conclusions 141
Protocol 5 1 145
Protocol 5 2 146
6 Quantitative real-time PCR 151
Cindy J Smith
6 1 Introduction 151
6 2 Quantification 152
6 3 qPCR chemistries 154
631 Taq nuclease assay 154
632 SYBR green 156
6 4 Applications 156
641 qPCR analysis of total microbial communities via analysis 156
of amplified ribosomal RN£ genes
642 Functional ecology * 157
Protocol 6 1 161
7 Stable-isotope probing 167
Stefan Radajewski and J: Colin Murrell
7 1 Introduction 167
7 2 Stable-isotope labeling of DNA 168
7 3 Application of stable-isotope probing 169
731 Methylotroph populations 169
732 Ammonia-oxidizing populations 170
7 4 Future prospects 170
Protocol 7 1 173
8 Applications of nucleic acid hybridization in microbial ecology 179
A Mark Osborn, Vivien Prior and Konstantinos Damianakis
8 1 Introduction 179
8 2 Fundamentals of DNA hybridization 180
821 Probe design 180
822 Choice of nucleic acid template 184
8 3 Hybridization applications in microbial ecology 186
831 Hybridization analysis of cultured bacteria 186
viii Contents
832 Hybridization analysis of total community DNA 187
833 Reverse sample genome probing (RSGP) and microarray 189
,r analysis of microbial communities
Protocol 8 1 198
Protocol 8 2 207
Protocol 8 3 211
Fluorescence in situ hybridization for the detection of prokaryotes 213
Holger Daims, Kilian Stoecker and Michael Wagner
9 1 Introduction 213
9 2 Fundamentals of rRNA FISH 214
9 3 Structure-function analyses: combination of FISH with other
techniques 217
Protocol 9 1 223
10 Lessons from the genomes: microbial ecology and genomics 241
Andrew S Whiteley, Mike Manefield, Sarah L Turner and Mark J Bailey
10 1 Introduction 241
10 2 Structural genomics 242
10 3 Comparative genomics 242
10 3 1 Small genomes 243
10 3 2 Large genomes 244
10 3 3 The horizontal gene pool 244
10 3 4 Insights into phylogeny 245
10 4 Functional genomics in microbfSl ecology 246
10 4 1 Characterizing microbial responses to environmental change 246
10 4 2 Relating specific genes to specific functions 247
10 4 3 Expression of genes from components of a genome 248
10 5 The application of genomic tools in situ 249
10 5 1 Organism diversity ~ 249
10 5 2 Functional genes 250
10 5 3 Metagenomics 250
10 5 4 Functional gene arrays 252
10 5 5 Diversity, function and process 253
10 6 Lessons learned and future perspectives 254
11 Metagenomic libraries from uncultured microorganisms 261
Doreen E Gillespie, Michelle R Rondon, Lynn L Williamson and Jo Handelsman
11 1 Introduction 261
11 2 Factors affecting experimental design 261
11 2 1 Environmental source 262
11 2 2 DNA isolation 264
11 2 3 Vector selection 264
11 2 4 Host cell selection 264
11 3 Summary 265
Protocol 11 1 269
Contents ix
12 A molecular toolbox for bacterial ecologists: PCR primers for
functional gene analysis
Michael J Larkin, A Mark Osborn and Derek Fairley
j-12 1 Introduction
12 2 Adopting a strategy to detect sequences from the environment
12 3 Primer and probe design
12 4 PCR primers to amplify 16S rRNA gene sequences
12 5 PCR primers for detecting functional genes
13 Molecular detection of fungal communities in soil 303
Eric Smit, Francisco de Souza and Renske Landeweert
13 1 Introduction 303
13 2 Molecular strategies for fungal community analyses 304
13 3 DNA extraction 305
13 4 Target genes 306
13 5 Primers for fungal populations 307
13 6 In silico analysis of 18S rRNA primers 310
13 7 Specific application of molecular identification techniques for studying 311
mycorrhizal fungi
13 7 1 Analyzing arbuscular mycorrhizal fungal diversity 311
13 7 2 Analyzing ectomycorrhizal fungal diversity 312
13 8 Conclusions 313
Protocol 13 1 317
14 Environmental assessment: bioreporter systems
Steven A Ripp and Gary S Sayler *
14 1 Introduction
14 2 Reporter systems
14 2 1 fJ-Galactosidase (lacZ)
14 2 2 Chloramphenicol acetyltransferase (CAT)
14 2 3 Catechol 2,3-dioxygenase (xylE)
14 2 4 BrLactamase (bid)
14 2 5 B-Glucorinidase (gusA, gurA, uidA)
14 2 6 Ice nucleation (inaZ)
14 2 7 Green fluorescent protein (GFP)
14 2 8 Aequorin
14 2 9 Uroporphyrinogen (Urogen) III methyltransferase (UMT)
14 2 10 Luciferases
14 3 Mini-transposons as genetic tools in biosensor construction
Case study
IS Bioinformatics and web resources for the microbial ecologist
Wolfgang Ludwig
15 1 Introduction
15 2 Databases
15 2 1 European Bioinformatics Institute (EBI) databases
15 2 2 GenBank
x Contents
15 3 Genome projects 359
° 15 3 1 Munich Information Center for Protein Sequences (MIPS) 359
15 3 2 Sanger Institute 359
S 15 3 3 The Institute for Genomic Research (TIGR) 359
15 3 4 Mobile genetic elements (MGE) databases 360
15 4 Ribosomal RNA databases 360
15 4 1 Ribosomal Database Project 360
15 4 2 European Ribosomal RNA database 362
15 5 rRNA-targeted probes 363
15 5 1 ProbeBase 363
15 5 2 Roscoff database 363
15 5 3 PRIMROSE 363
15 6 ARB project 363
15 6 1 ARB databases 364
15 6 2 Phylogenetic treeing 366
15 6 3 Future developments 369
15 7 Concluding remarks 369
Index 373
|
| adam_txt |
Molecular Microbial
Ecology
Dr A Mark Osborn (Ed )
Department of Animal amp; Plant Sciences, University of Sheffield,
Sheffield, UK
Dr Cindy J Smith (Ed )
Department of Biological Sciences, University of Essex,
Colchester, UK
Taylor amp; Francis
Taylor amp; Francis Group
Contents
Contributors xi
Abbreviations xii
Preface xiii
1 Nucleic acid extraction from environmental samples 1
Annett Milling, Newton C M Gomes, Miruna Oros-Sichler, Monika Gb'tz and
Kornelia Smalla
1 1 Introduction 1
1 2 Recovery of cells from environmental matrices 2
1 3 Cell lysis and DNA extraction protocols 3
1 4 Immunochemical isolation of DNA from metabolically active cells 5
1 5 RNA extraction from environmental matrices 6
Protocol 1 1 11
Protocol 1 2 17
Protocol 13_ 21
Prokaryotic systematic^: PCR and sequence analysis of amplified 25
16S rRNA genes
Wilfred F M Roling and Ian M Head
2 1 Introduction - 25
2 2 Application of PCR in microbial ecology 26
221 Good working practices in PCR: avoiding PCR inhibition and 28
contamination
222 Pitfalls of PCR: artifacts and differential amplification 30
223 rRNA versus rDNA: indicative of active versus total microbial 32
communities?
2 3 Generation and analysis of clone libraries 34
231 Generation of clone libraries 34
232 Screening of clone libraries 35
2 4 Quantitative analysis of clone libraries: coverage and diversity indices 38
241 Coverage and sampling 38
242 Diversity indices 40
2 5 Phylogenetic inference 41
251 Sequencing 41
252 Phylogenetic analysis 42
253 Parsimony 44
254 Distance methods 46
vi Contents
255 Maximum likelihood 47
256 Bootstrap analysis 50
,26 Phylogenetic analysis compared to other molecular techniques 50
^261 Comparing large numbers of samples 51
262 Quantification 51
263 Relationship between phylogenetic information and function 52
2 7 Concluding remarks 52
Protocol 2 1 57
3 DNA fingerprinting of microbial communities 65
Andreas Felske and A Mark Osborn
3 1 Introduction 65
311 DGGE 66
312 TGGE 68
313 SSCP analysis 68
314 T-RFLP analysis 69
315 LH-PCR (length heterogeneity PCR) 70
316 Comparison of the different methods 71
3 2 Applications of microbial community fingerprinting 74
321 Combination of cloning with fingerprinting 76
322 Screening isolated strains next to fingerprints 76
323 Southern blot hybridization and fingerprints 77
324 Multiple competitor RT-PCR/TGGE for rRNA 78
325 Environmental fingerprints with protein-coding genes 79
3 3 Analyzing microbial community fingerprints 79
331 Cutting out fingerprint bands for sequencing 80
332 The rRNA sequence hetejogeneity 81
333 Quantitative analysis of fingerprint signals 82
334 Cluster analysis of DNA fingerprint signals 84
335 Diversity and species richness 84
336 The 'operational taxonomic unit' 85
3 4 Miscellaneous-advice 85
Protocol 3 1 91
4 Molecular typing of environmental isolates 97
Jan L W Rademaker, Henk J M Aarts and Pablo Vinuesa
4 1 Introduction 97
411 Role of PCR amplification-based typing for microbial ecology 98
4 2 Microbial typing methods 98
421 AFLP analysis 99
422 rep-PCR fingerprinting 103
423 Mini- and microsatellite fingerprinting 106
424 Fingerprinting methods using random primers 106
(RAPD, AP-PCR, DAF)
425 Ribosomal RNA gene fingerprinting methods 108
4 3 Computer-assisted analysis of PCR-generated fingerprint profiles 113
4 4 Concluding remarks 114
Protocol 4 1 127
Protocol 4 2 131
Contents vii
RT-PCR and mRNA expression analysis of functional genes 135
Balbina Nogales
P5 1 Introduction 135
-^ 5 2 Advantages and limitations of the RT-PCR analysis of bacterial 136
functional genes
5 3 The RT-PCR reaction 137
531 The RNA template 137
532 Primers for reverse transcription 138
533 Reverse transcriptases 138
534 One-step and two-step RT-PCR protocols 139
5 4 Quantitative RT-PCR 140
5 5 Analysis of global gene expression 140
5 6 Conclusions 141
Protocol 5 1 145
Protocol 5 2 146
6 Quantitative real-time PCR 151
Cindy J Smith
6 1 Introduction 151
6 2 Quantification 152
6 3 qPCR chemistries 154
631 Taq nuclease assay 154
632 SYBR green 156
6 4 Applications 156
641 qPCR analysis of total microbial communities via analysis 156
of amplified ribosomal RN£ genes
642 Functional ecology * 157
Protocol 6 1 161
7 Stable-isotope probing 167
Stefan Radajewski and J: 'Colin Murrell
7 1 Introduction 167
7 2 Stable-isotope labeling of DNA 168
7 3 Application of stable-isotope probing 169
731 Methylotroph populations 169
732 Ammonia-oxidizing populations 170
7 4 Future prospects 170
Protocol 7 1 173
8 Applications of nucleic acid hybridization in microbial ecology 179
A Mark Osborn, Vivien Prior and Konstantinos Damianakis
8 1 Introduction 179
8 2 Fundamentals of DNA hybridization 180
821 Probe design 180
822 Choice of nucleic acid template 184
8 3 Hybridization applications in microbial ecology 186
831 Hybridization analysis of cultured bacteria 186
viii Contents
832 Hybridization analysis of total community DNA 187
833 Reverse sample genome probing (RSGP) and microarray 189
,r analysis of microbial communities
Protocol 8 1 198
Protocol 8 2 207
Protocol 8 3 211
Fluorescence in situ hybridization for the detection of prokaryotes 213
Holger Daims, Kilian Stoecker and Michael Wagner
9 1 Introduction 213
9 2 Fundamentals of rRNA FISH 214
9 3 Structure-function analyses: combination of FISH with other
techniques 217
Protocol 9 1 223
10 Lessons from the genomes: microbial ecology and genomics 241
Andrew S Whiteley, Mike Manefield, Sarah L Turner and Mark J Bailey
10 1 Introduction 241
10 2 Structural genomics 242
10 3 Comparative genomics 242
10 3 1 Small genomes 243
10 3 2 Large genomes 244
10 3 3 The horizontal gene pool 244
10 3 4 Insights into phylogeny 245
10 4 Functional genomics in microbfSl ecology 246
10 4 1 Characterizing microbial responses to environmental change 246
10 4 2 Relating specific genes to specific functions 247
10 4 3 Expression of genes from components of a genome 248
10 5 The application of genomic tools in situ 249
10 5 1 Organism diversity ~ 249
10 5 2 Functional genes 250
10 5 3 Metagenomics 250
10 5 4 Functional gene arrays 252
10 5 5 Diversity, function and process 253
10 6 Lessons learned and future perspectives 254
11 Metagenomic libraries from uncultured microorganisms 261
Doreen E Gillespie, Michelle R Rondon, Lynn L Williamson and Jo Handelsman
11 1 Introduction 261
11 2 Factors affecting experimental design 261
11 2 1 Environmental source 262
11 2 2 DNA isolation 264
11 2 3 Vector selection 264
11 2 4 Host cell selection 264
11 3 Summary 265
Protocol 11 1 269
Contents ix
12 A molecular toolbox for bacterial ecologists: PCR primers for
functional gene analysis
Michael J Larkin, A Mark Osborn and Derek Fairley
j-12 1 Introduction
12 2 Adopting a strategy to detect sequences from the environment
12 3 Primer and probe design
12 4 PCR primers to amplify 16S rRNA gene sequences
12 5 PCR primers for detecting functional genes
13 Molecular detection of fungal communities in soil 303
Eric Smit, Francisco de Souza and Renske Landeweert
13 1 Introduction 303
13 2 Molecular strategies for fungal community analyses 304
13 3 DNA extraction 305
13 4 Target genes 306
13 5 Primers for fungal populations 307
13 6 In silico analysis of 18S rRNA primers 310
13 7 Specific application of molecular identification techniques for studying 311
mycorrhizal fungi
13 7 1 Analyzing arbuscular mycorrhizal fungal diversity 311
13 7 2 Analyzing ectomycorrhizal fungal diversity 312
13 8 Conclusions 313
Protocol 13 1 317
14 Environmental assessment: bioreporter systems
Steven A Ripp and Gary S Sayler *
14 1 Introduction
14 2 Reporter systems
14 2 1 fJ-Galactosidase (lacZ)
14 2 2 Chloramphenicol acetyltransferase (CAT)
14 2 3 Catechol 2,3-dioxygenase (xylE)
14 2 4 BrLactamase (bid)
14 2 5 B-Glucorinidase (gusA, gurA, uidA)
14 2 6 Ice nucleation (inaZ)
14 2 7 Green fluorescent protein (GFP)
14 2 8 Aequorin
14 2 9 Uroporphyrinogen (Urogen) III methyltransferase (UMT)
14 2 10 Luciferases
14 3 Mini-transposons as genetic tools in biosensor construction
Case study
IS Bioinformatics and web resources for the microbial ecologist
Wolfgang Ludwig '
15 1 Introduction
15 2 Databases
15 2 1 European Bioinformatics Institute (EBI) databases
15 2 2 GenBank
x Contents
15 3 Genome projects 359
° 15 3 1 Munich Information Center for Protein Sequences (MIPS) 359
15 3 2 Sanger Institute 359
S 15 3 3 The Institute for Genomic Research (TIGR) 359
15 3 4 Mobile genetic elements (MGE) databases 360
15 4 Ribosomal RNA databases 360
15 4 1 Ribosomal Database Project 360
15 4 2 European Ribosomal RNA database 362
15 5 rRNA-targeted probes 363
15 5 1 ProbeBase 363
15 5 2 Roscoff database 363
15 5 3 PRIMROSE 363
15 6 ARB project 363
15 6 1 ARB databases 364
15 6 2 Phylogenetic treeing 366
15 6 3 Future developments 369
15 7 Concluding remarks 369
Index 373 |
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| id | DE-604.BV021283594 |
| illustrated | Illustrated |
| index_date | 2024-07-02T13:47:51Z |
| indexdate | 2024-07-09T20:34:40Z |
| institution | BVB |
| isbn | 1859962831 |
| language | English |
| lccn | 2005014409 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014604553 |
| oclc_num | 60414717 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| physical | XVI, 381 S. graph. Darst. cm |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Taylor & Francis |
| record_format | marc |
| series2 | BIOS advanced methods |
| spelling | Molecular microbial ecology A. Mark Osborn and Cindy Smith (eds) New York, NY [u.a.] Taylor & Francis 2005 XVI, 381 S. graph. Darst. cm txt rdacontent n rdamedia nc rdacarrier BIOS advanced methods Includes bibliographical references and index Microbiologie moléculaire Écologie microbienne Ökologie Molecular microbiology Microbial ecology Ecology Environmental Microbiology Nucleic Acid Hybridization Polymerase Chain Reaction methods Ökologie (DE-588)4043207-5 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 s Mikrobiologie (DE-588)4316357-9 s Ökologie (DE-588)4043207-5 s DE-604 Mikroorganismus (DE-588)4039226-0 s b DE-604 Osborn, A. Mark Sonstige oth Smith, Cindy J. Sonstige oth http://www.loc.gov/catdir/toc/ecip0513/2005014409.html Table of contents HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014604553&sequence=000006&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular microbial ecology Microbiologie moléculaire Écologie microbienne Ökologie Molecular microbiology Microbial ecology Ecology Environmental Microbiology Nucleic Acid Hybridization Polymerase Chain Reaction methods Ökologie (DE-588)4043207-5 gnd Molekularbiologie (DE-588)4039983-7 gnd Mikroorganismus (DE-588)4039226-0 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| subject_GND | (DE-588)4043207-5 (DE-588)4039983-7 (DE-588)4039226-0 (DE-588)4316357-9 |
| title | Molecular microbial ecology |
| title_auth | Molecular microbial ecology |
| title_exact_search | Molecular microbial ecology |
| title_exact_search_txtP | Molecular microbial ecology |
| title_full | Molecular microbial ecology A. Mark Osborn and Cindy Smith (eds) |
| title_fullStr | Molecular microbial ecology A. Mark Osborn and Cindy Smith (eds) |
| title_full_unstemmed | Molecular microbial ecology A. Mark Osborn and Cindy Smith (eds) |
| title_short | Molecular microbial ecology |
| title_sort | molecular microbial ecology |
| topic | Microbiologie moléculaire Écologie microbienne Ökologie Molecular microbiology Microbial ecology Ecology Environmental Microbiology Nucleic Acid Hybridization Polymerase Chain Reaction methods Ökologie (DE-588)4043207-5 gnd Molekularbiologie (DE-588)4039983-7 gnd Mikroorganismus (DE-588)4039226-0 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| topic_facet | Microbiologie moléculaire Écologie microbienne Ökologie Molecular microbiology Microbial ecology Ecology Environmental Microbiology Nucleic Acid Hybridization Polymerase Chain Reaction methods Molekularbiologie Mikroorganismus Mikrobiologie |
| url | http://www.loc.gov/catdir/toc/ecip0513/2005014409.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014604553&sequence=000006&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT osbornamark molecularmicrobialecology AT smithcindyj molecularmicrobialecology |