Analysis and detection by capillary electrophoresis:
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| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier
2005
|
| Edition: | 1. ed. |
| Series: | Wilson & Wilson's comprehensive analytical chemistry
45 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXVIII, 767 S. Ill., graph. Darst. |
| ISBN: | 0444517189 |
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MARC
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| _version_ | 1804133421932347393 |
|---|---|
| adam_text | Titel: Analysis and detection by capillary electrophoresis
Autor: Marina, Maria Luisa
Jahr: 2005
Contents
Contributors to Vol XLV........................................................................vii
Volumes in the Series............................................................................xi
Series Editor s Preface..........................................................................xxv
Preface.................................................xxvii
Chapter 1. Fundamentals of capillary electrophoresis
Maria Luisa Marina, Angel Rios, Miguel Valcarcel
1.1 Introduction........................................................................1
1.2 Basic Concepts....................................................................2
1.2.1 Electrophoretic mobility and electroosmotic flow 2
1.2.2 Migration time, efficiency, and resolution............4
1.2.3 Experimental variables affecting the
electrophoretic resolution....................................6
1.3 Instrumentation..................................................................11
1.3.1 High-voltage power supply..................................12
1.3.2 Injection systems................................................12
1.3.3 Capillaries..........................................................14
1.3.4 Detection systems..............................................18
1.4 Separation Modes................................................................19
1.5 Hyphenated Capillary Electrophoretic Techniques................21
1.6 Field of Application and Trends..........................................23
1.6.1 Role of CE techniques with respect to the
analytical potential of other separation techniques 23
1.6.2 Field of application of CE....................................24
1.6.3 Trends..............................................................27
References....................................................................................28
Chapter 2. Separation modes in capillary electrophoresis
Maria Angeles Garcia, Maria Luisa Marina, Angel Rios, Miguel
Valcarcel
2.1 Introduction........................................................................31
2.2 Separation Modes in CE Based on Pure Electrophoretic
Principles............................................................................34
xv
Contents
2.2.1 Capillary zone electrophoresis........-..........34
2.2.2 Capillary gel electrophoresis..............................45
2.2.3 Capillary isoelectric focusing..............................50
2.2.4 Capillary isotachophoresis..................................56
2.3 Separation Modes in CE Combining Electrophoretic and
Chromatographic Principles................................................50
2.3.1 Electrokinetic chromatography..........................60
2.3.2 Capillary electrochromatography........................95
2.4 Nonaqueous Capillary Electrophoresis..................................108
2.4.1 Introduction......................................................108
2.4.2 Electrolytic solutions..........................................110
2.4.3 Analyte-additive interactions..............................112
2.4.4 Methodological aspects........................................114
2.4.5 Applications........................................................116
2.5 Selection of the CE Mode and Separation Conditions............118
Acknowledgments..........................................................................118
References....................................................................................122
Chapter 3. On-line sample preconcentration techniques in capillary
electrophoresis
Hong Chen, Shigeru Terabe
3.1 Introduction....................................
3.2 Sample Stacking Technique......................... *37
3.2.1 Basic principle of stacking................. 137
3.2.2 Normal stacking mode........................139
3.2.3 Large-volume sample stacking.............. 140
3.2.4 Electrokinetic injection................... 142
3.2.5 pH-mediated stacking.................... 143
3.2.6 Sample stacking of analytes in micellar
electrokinetic chromatography.............. 143
3.2.7 Applications of sample stacking............. 147
3.3 ITP Preconcentration............................. 147
3.3.1 Principle of ITP......................... 147
3.3.2 Normal ITP-zone electrophoresis
mode................................. 150
3.3.3 Transient ITP mode..........................153
3.3.4 Applications of ITP preconcentration
technique.............................. 154
xvi
Contents
3.4 Dynamic pH Junction Preconcentration..............................154
3.4.1 Principle of dynamic pH junction........................154
3.4.2 Applications of dynamic pH junction..................158
3.5 Sweeping..............................................................................159
3.5.1 Basic theory of sweeping....................................159
3.5.2 Selective exhaustive injection-sweeping..............163
3.5.3 Dynamic pH junction sweeping..........................164
3.5.4 Applications of sweeping....................................165
References....................................................................................166
Chapter 4. Coupling continuous flow systems to capillary
electrophoresis
Bartolome M. Simonet, Angel Rios, Miguel Valcarcel
4.1 Introduction........................................................................173
4.2 Role of CFSs........................................................................174
4.3 Interfaces for Coupling Flow Systems to CE........................175
4.3.1 CFSs to homemade CE arrangements................176
4.3.2 Coupling flow systems to commercial CE
equipments........................................................179
4.3.3 Flow injection-CE integrated microchips............182
4.4 Sample Injection and On-line Preparation............................185
4.4.1 On-line clean-up and preconcentration................185
4.4.2 Online extraction/filtering..................................189
4.4.3 On-line dialysis..................................................190
4.4.4 On-line membrane and hollow fibre system .... 192
4.4.5 On-line gas extraction and gas diffusion..............194
4.4.6 On-line sample treatment and separations
performed in auxiliary equipments....................194
4.5 Automatic Calibration in CE through CFS..........................200
4.6 Screening Flow Units-CE Analysis
Arrangements ....................................................................204
4.7 Analytical Applications by CFS-CE......................................207
4.7.1 Biomedical and pharmaceutical analysis..............207
4.7.2 Environmental analysis......................................213
4.7.3 Food analysis......................................................216
4.8 Future Prospects..................................................................219
Acknowledgements........................................................................220
References....................................................................................220
xvii
Contents
Chapter 5. UV-Vis absorbance detection in capillary electrophoresis
Antonio L. Crego, Maria Luisa Marina
5.1 Introduction........................................................................225
5.2 UV-Vis Absorbance Detection in CE....................................231
5.2.1 Basic principles..................................................231
5.2.2 Detector design..................................................232
5.2.3 Derivatization and complexation
procedures..........................................................261
5.2.4 Indirect absorbance detection............................269
5.3 Thermooptical Detection in CE............................................278
5.3.1 Thermal lens detection......................................278
5.3.2 Photoacoustic detection......................................287
5.4 UV-Vis Absorbance Detection in Microchip
electrophoresis . .................................................................291
Acknowledgments..............................................................296
References........................................................................296
Chapter 6. Fluorescence detection in capillary electrophoresis
Maria Teresa Veledo, Pilar Lara-Quintanar, Mercedes de Frutos,
Jose Carlos Diez-Masa
6.1 Introduction........................................................................205
6.1.1 Objectives and outline of this
chapter..............................................................305
6.1.2 Basic concepts..........................................306
6.2 Instrumental Setup..............................................................307
6.2.1 Light sources..............................307
6.2.2 Optical train for LIF..........................................310
6.2.3 Light measuring devices for LIF........................314
6.3 Derivatization Methods........................................................320
6.3.1 Pre-capillary derivatization................................321
6.3.2 On-capillaiy derivatization..................................326
6.3.3 Post-capillary derivatization................................328
6.3.4 Protein detection with and without
derivatization......................................................329
6.4 Miniaturized Fluorescence Detection for
Microchips............................................................335
6.4.1 On-chip fluorescence detection............................340
6.4.2 Miniaturized fluorescence detection....................343
xvm
Contents
6.5 Indirect Fluorescence Detection............................................347
6.5.1 Background concepts: transfer ratio (TR) and
dynamic reserve (DR)........................................347
6.5.2 Choice of the background electrolyte (BGE): types
of electrolyte additives used in indirect
fluorescence detection........................................349
6.5.3 Direct versus indirect fluorescence detection . . . 351
6.5.4 Applications of indirect fluorescence detection . . 351
6.6 Conclusions and Future Trends............................................353
6.6.1 Multiphoton fluorescence detection....................357
6.6.2 Wavelength-resolved LIF detection....................359
6.6.3 Time-resolved LIF..............................................361
6.6.4 New approaches to fluorescent labeling of proteins
at low concentrations..........................................363
6.7 Selected Applications............................................................363
Acknowledgments..........................................................................364
References....................................................................................364
Chapter 7. Novel optical detection techniques in CE based on
phosphorescence or chemiluminescence
Jacobus Kuijt, Freek Ariese, Cees Gooijer
7.1 Introduction........................................................................375
7.2 Phosphorescence Detection in CE........................................376
7.2.1 Introduction......................................................376
7.2.2 Basic features of phosphorescence......................377
7.2.3 Coupling of phosphorescence detection
to CE..................................................................379
7.2.4 Applications of CE-phosphorescence..................380
7.2.5 Concluding remarks on
CE-phosphorescence..........................................385
7.3 Chemiluminescence Detection in CE....................................388
7.3.1 Introduction......................................................388
7.3.2 Development of CL detection in CE....................388
7.3.3 CL reactions......................................................389
7.3.4 Post-capillary CL reactors..................................394
7.3.5 Applications of CE-CL........................................396
7.3.6 Concluding remarks on CE-CL..........................407
References....................................................................................408
xix
Contents
Chapter 8. Electrochemical detection in capillary
electrophoresis
Kim M. Hanson, Theron J. Pappas, Lisa A. Holland
8.1 Introduction..............................................413
8.2 Modes of EC Detection........................................................414
8.2.1 Amperometry......................................................414
8.2.2 Voltammetry......................................................419
8.2.3 Conductivity......................................................421
8.2.4 Potentiometry....................................................422
8.3 Decoupling..........................................................................422
8.4 Applications........................................................................424
8.4.1 Neurochemistry..................................................425
8.4.2 Biomarkers........................................................427
8.4.3 Physiological activity..........................................428
8.4.4 Environmental monitoring..................................428
8.5 Conclusions and Future Prospects........................................430
Acknowledgments..................................... 431
References.......................................... 431
C/iap(er 9. Mass spectrometry detection in capillary
electrophoresis
Carolina Simo, Alejandro Cifuentes
9.1 Introduction........................................................................441
9.2 Principles and Instrumentation............................................442
9.2.1 Electrospray ionization......................................443
9.2.2 Mass analyzers..................................................452
9.2.3 CE-MS compatibility..........................................453
9.2.4 Chip-based CE-MS............................................477
9.3 Applications........................................................................481
9.3.1 Proteins and peptides........................................481
9.3.2 Drugs and metabolites........................................487
9.3.3 Pesticides............................................................494
9.3.4 Other compounds..............................................498
9.4 Future Outlook....................................................................504
Acknowledgments.........................................................................505
References....................................................................................505
xx
Contents
Chapter 10. Inductively coupled plasma-mass spectrometry for specific
detection in capillary electrophoresis
Gloria Alvarez Llamas, Ma del Rosario Fernandez de la
Campa, Elisa Blanco Gonzalez, Alfredo Sanz Medel
10.1 The Inductively Coupled Plasma-mass Spectrometry
(ICP-MS) as a specific atomic detector................ 519
10.1.1 ICP-MS fundamentals................... 519
10.1.2 ICP-MS as detection system in hyphenated
techniques............................ 522
10.2 Interfacing CE to ICP-MS: Potentials and
Limitations.................................... 524
10.3 CE-ICP-MS Coupling via Nebulisation............... 525
10.3.1 The interface: key to success.............. 525
10.3.2 Considerations about sensitivity/detection limits
in CE-ICP-MS......................... 529
10.3.3 The importance of the selected nebuliser in
CE-ICP-MS couplings................... 530
10.4 CE-ICP-MS Coupling via Volatile Species
generation..................................... 545
10.4.1 Advantages of volatile species generation in
CE-ICP couplings...................... 546
10.4.2 Interface designs based on VSG............ 546
10.5 Strategies to Improve Sensitivity in CE-ICP-MS
Coupling...................................... 548
10.6 Some Selected Contributions and Applications.......... 550
10.7 Conclusions and Future Prospects................... 552
References.......................................... 552
Chapter 11. Vibrational spectroscopic detection in capillary
electrophoresis (CE)
Stephen Kulka, Bernhard Lendl
11.1 Introduction................................... 557
11.2 IR Spectroscopy................................. 558
11.2.1 On-line MIR spectroscopic detection
in CE................................ 558
11.2.2 FTIR spectroscopic detection in CE after solvent
elimination........................... 563
xxi
Contents
11.3 Raman Spectroscopy............................. 566
11.3.1 Raman scattering in CE.................. 567
11.3.2 Surface-enhanced Raman scattering in CE. . . .
References
573
580
583
Chapter 12. Coupling nuclear magnetic resonance to capillary
eletrophoresis
Dimuthu A. Jayawickrama, Jonathan V. Sweedler
12.1 Introduction..................................
12.1.1 NMR sensitivity improvements........................584
12.1.2 CE and NMR hyphenation................................585
12.2 CE-NMR Hyphenation......................................................586
12.2.1 The size-matched NMR probe..........................586
12.2.2 Interfacing CE to NMR....................................590
12.2.3 CE-NMR measurements..................................592
12.3 Applications........................................................................597
12.3.1 CZE-NMR........................................................598
12.3.2 Sample concentration methods..........................600
12.3.3 Diagnostic capabilities of CE/cITP-NMR..........607
12.4 Conclusions and Future Directions....................................613
References....................................................................................614
Chapter 13. Chiral analysis by capillary electrophoresis
Carmen Garcia-Ruiz, Maria Luisa Marina
13.1 Introduction................................... 017
13.2 Separation Modes in CE for Chiral Analysis........... 022
13.2.1 Electrokinetic chromatography............. 023
13.2.2 Capillary electrochromatography........... 039
13.2.3 Non-aqueous capillary electrophoresis....... 040
13.3 Improving the Detection Sensitivity for Chiral
Analysis by CE................................. 645
13.3.1 On-line preconcentration techniques employed
in chiral analysis by CE.................. 647
13.3.2 Alternative detection systems to on-line UV
detection employed in chiral analysis
xxii
Contents
13.4 Applications of Chiral Analysis by CE.......................656
13.4.1 Pharmaceutical and biomedical
analysis............................................................656
13.4.2 Environmental analysis....................................668
13.4.3 Food analysis....................................................679
13.4.4 Other applications............................................686
13.5 Chiral Analysis by Microchip Electrophoresis....................687
13.6 Future Perspectives............................................................689
Acknowledgments..........................................................................692
References....................................................................................692
Chapter 14. Electrochemical detection in capillary electrophoresis on
microchips
Antonio Javier Blasco, Alberto Escarpa
14.1 Introduction......................................................................703
14.2 Principles and Designs of Capillary Electrophoresis with
Electrochemical Detection Microsystems............................707
14.2.1 Materials and microfabrication
techniques........................................................708
14.2.2 Fluids moving: principles of injection................713
14.2.3 Detection implementation................................716
14.2.4 Electrode materials..........................................730
14.2.5 Modes of detection............................................734
14.3 Selected Analytical Applications........................................741
14.3.1 Neurotransmitters and related
compounds........................................................741
14.3.2 Bioanalytical and clinical..................................742
14.3.3 Environmental..................................................746
14.3.4 Conductimetry..................................................749
14.4 Conclusions and Future Direction......................................752
Acknowledgements........................................................................754
References....................................................................................754
List of Abbreviations....................................................................759
Subject Index................................................................................761
xxiii
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| illustrated | Illustrated |
| indexdate | 2024-07-09T20:08:27Z |
| institution | BVB |
| isbn | 0444517189 |
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| series | Wilson & Wilson's comprehensive analytical chemistry |
| series2 | Wilson & Wilson's comprehensive analytical chemistry |
| spelling | Analysis and detection by capillary electrophoresis ed. by M. L. Marina... 1. ed. Amsterdam [u.a.] Elsevier 2005 XXVIII, 767 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Wilson & Wilson's comprehensive analytical chemistry 45 Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 s DE-604 Marina, Maria L. Sonstige oth Wilson & Wilson's comprehensive analytical chemistry 45 (DE-604)BV000900805 45 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013212420&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Analysis and detection by capillary electrophoresis Wilson & Wilson's comprehensive analytical chemistry Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4290002-5 |
| title | Analysis and detection by capillary electrophoresis |
| title_auth | Analysis and detection by capillary electrophoresis |
| title_exact_search | Analysis and detection by capillary electrophoresis |
| title_full | Analysis and detection by capillary electrophoresis ed. by M. L. Marina... |
| title_fullStr | Analysis and detection by capillary electrophoresis ed. by M. L. Marina... |
| title_full_unstemmed | Analysis and detection by capillary electrophoresis ed. by M. L. Marina... |
| title_short | Analysis and detection by capillary electrophoresis |
| title_sort | analysis and detection by capillary electrophoresis |
| topic | Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Kapillarelektrophorese |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013212420&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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| work_keys_str_mv | AT marinamarial analysisanddetectionbycapillaryelectrophoresis |