The proteomics protocols handbook:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2005
|
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis |
| Beschreibung: | XVIII, 988 S. Ill., graph. Darst. |
| ISBN: | 1588293432 1588295931 |
Internformat
MARC
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| 245 | 1 | 0 | |a The proteomics protocols handbook |c ed. by John M. Walker |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2005 | |
| 300 | |a XVIII, 988 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Eiwitsynthese |2 gtt | |
| 650 | 7 | |a Eiwitten |2 gtt | |
| 650 | 4 | |a Protéomique | |
| 650 | 7 | |a Transcriptiefactoren |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Proteomics |v Handbooks, manuals, etc | |
| 650 | 4 | |a Proteomics |x methods | |
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| 700 | 1 | |a Walker, John M. |e Sonstige |4 oth | |
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Datensatz im Suchindex
| _version_ | 1804133393506500608 |
|---|---|
| adam_text | Contents
Preface vii
Contributors xiii
1 Extraction and Solubilization of Proteins for Proteomic Studies
Richard M. Leimgruber 1
2 Preparation of Bacterial Samples for 2 D PAGE
Brian Berg Vandahl, Gunna Christiansen, and Svend Birkelund 19
3 Preparation of Yeast Samples for 2 D PAGE
Joakim Norbeck 27
4 Preparation of Mammalian Tissue Samples
for Two Dimensional Electrophoresis
Frank A. Witzmann 31
5 Differential Detergent Fractionation of Eukaryotic Cells
Melinda L. Ramsby and Gregory S. Makowski 37
6 Serum or Plasma Sample Preparation for Two Dimensional Gel Electrophoresis
Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari,
and Craig D. Shriver 49
7 Preparation of Plant Protein Samples for 2 D PAGE
David W. M. Leung 55
8 Laser Assisted Microdissection in Proteomic Analyses
Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan,
Henry Brzeski, Richard I. Somiari, and Craig D. Shriver 59
9 Purification of Cellular and Organelle Populations
by Fluorescence Activated Cell Sorting for Proteome Analysis
William L. Godfrey, Colette J. Rudd, Sujata Iyer,
and Diether Recktenwald 67
10 Purification of Nucleoli From Lymphoma Cells and Solubilization
of Nucleolar Proteins for 2 DE Separation
Regis Dieckmann, Yohann Coute, Denis Hochstrasser,
Jean Jacques Diaz, and Jean Charles Sanchez 79
11 Prefractionation of Complex Protein Mixture for 2 D PAGE
Using Reversed Phase Liquid Chromatography
Volker Badock and Albrecht Otto 87
12 Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing
Using ZOOM™ IEF Fractionators to Improve Protein Profiling
Xun Zuo, Ki Boom Lee, and David W. Speicher 97
13 Large Format 2 D Polyacrylamide Gel Electrophoresis
Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari,
and Craig D. Shiver 119
14 Analysis of Membrane Proteins by Two Dimensional Gels
Michael Fountoulakis 133
viii Contents
1 5 2 D PAGE of High Molecular Mass Proteins
Masamichi Oh lshi and Tadakazu Maeda 145
1 6 Using Ultra Zoom Gels for High Resolution Two Dimensional
Polyacrylamide Gel Electrophoresis
Sjouke Hoving, Hans Voshol, and Jan van Oostrum 151
1 7 NEpHGE and pi Strip Proteomic 2 D Gel Electrophoretic Mapping
of Lipid Rich Membranes
Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal,
and Christopher M. Taylor 167
18 Silver Staining of 2 D Gels
Julia Poland, Thierry Rabilloud, and Pranav Sinha 177
19 Zn2+ Reverse Staining Technique
Carlos Fernandez Patron 185
20 Multiplexed Proteomics Technology for the Fluorescence Detection
of Glycoprotein Levels and Protein Expression Levels Using Pro Q®
Emerald and SYPRO® Ruby Dyes
Birte Schulenberg and Wayne F. Patton 193
21 Multiplexed Proteomics Technology for the Fluorescence Detection
of Phosphorylation and Protein Expression Levels Using Pro Q®
Diamond and SYPRO® Ruby Dyes
Birte Schulenberg, Terrie Goodman, Thomas H. Steinberg,
and Wayne F. Patton 201
22 Sensitive Quantitative Fluorescence Detection of Proteins in Gels
Using SYPRO® Ruby Protein Gel Stain
Birte Schulenberg, Nancy Ahnert, and Wayne F. Patton 209
23 Rapid, Sensitive Detection of Proteins in Minigels With Fluorescent Dyes:
Coomassie Fluor Orange, SYPRO® Orange, SYPRO Red,
and SYPRO Tangerine Protein Gel Stains
Thomas H. Steinberg, Courtenay R. Hart, and Wayne F. Patton 215
24 Differential In Gel Electrophoresis in a High Throughput Environment
Richard I. Somiari, Stephen Russell, Stella B. Somiari, Anthony G. Sullivan,
Darrell L. Ellsworth, Henry Brzeski, and Craig D. Shriver 223
25 Statistical Analysis of 2 D Gel Patterns
Francoise Seillier Moiseiwitsch 239
26 2 DE Databases on the World Wide Web
Christine Hoogland, Khaled Mostaguir, and Ron D. Appel 259
27 Computer Analysis of 2 D Images
Patricia M. Palagi, Daniel Walther, Gerard Bouchet, Sonja Voordijk,
and Ron D. Appel 267
28 Comparing 2 D Electrophoretic Gels Across Internet Databases:
An Open Source Application
Peter F. Lemkin, Gregory C. Thornwall, and Jai Evans 279
29 Sample Cleanup by Solid Phase Extraction/Pipet Tip Chromatography
Alastair Aitken 307
30 Protein Identification by In Gel Digestion and Mass Spectrometric Analysis
Michele Learmonth and Alastair Aitken 311
Contents ix
31 Peptide Sequences of 2 D Gel Separated Protein Spots
by Nanoelectrospray Tandem Mass Spectrometry
Alastair Aitken 375
32 Identification of Proteins by MALDI TOF MS
Alastair Aitken 319
33 Sequencing of Tryptic Peptides Using Chemically Assisted Fragmentation
and MALDI PSD
John Flensburg and Maria Liminga 325
34 The In Situ Characterization of Membrane Immobilized
2 D PAGE Separated Proteins Using Ink Jet Technology
Patrick W. Cooley, Janice L. Joss, Femia G. Hopwood, Nichole L. Wilson,
and Andrew A. Gooley 341
35 Protein Identification by Peptide Mass Fingerprinting
Alastair Aitken 355
36 Analysis of the Proteomes in Human Tissues by In Gel Isoelectric Focusing
and Mass Spectrometry
Francesco Giorgianni and Sarka Beranova Giorgianni 367
37 Liquid Chromatography Coupled to MS for Proteome Analysis
Alastair Aitken 375
38 Quantitative Analysis of Proteomes and Subproteomes by Isotope Coded
Affinity Tags and Solid Phase Glycoprotein Capture
Eugene Yi, Hui Zhang, Kelly Cooke, Ruedi Aebersold,
and David R. Goodlett 385
39 Amino Acid Coded Mass Tagging for Quantitative Profiling
of Differentially Expressed Proteins and Modifications in Cells
XianChen 393
40 Mass Coded Abundance Tagging for Protein Identification
and Relative Abundance Determination in Proteomic Experiments
Gerard Cagney and Andrew Emili 407
41 Virtual 2 D Gel Electrophoresis by MALDI Mass Spectrometry
Angela K. Walker, Gary Rymar, and Philip C. Andrews 417
42 Identification of Posttranslational Modification by Mass Spectrometry
Alastair Aitken 431
43 Approaches to the O Glycoproteome
Franz Georg Hanisch and Stefan Miiller 439
44 Identification of Protein Phosphorylation Sites by Mass Spectrometry
Alastair Aitken 459
45 Quantitative Analysis of Protein Phosphorylation Status and Protein Kinase
Activity on Microassays Using Pro Q™ Diamond Dye Technology
Karen Martin and Wayne F. Patton 467
46 New Challenges and Strategies for Multiple Sequence Alignment
in the Proteomics Era
Julie D. Thompson and Olivier Poch 475
47 The Clustal Series of Programs for Multiple Sequence Alignment
Julie D. Thompson 493
x Contents
48 FASTA Servers for Sequence Similarity Search
Biju Issac and Gajendra P. S. Raghava 503
49 Protein Sequence Analysis and Domain Identification
Chris P. Ponting and Ewan Birney 527
50 Mammalian Genes and Evolutionary Genomics
Leo Goodstadt and Chris P. Ponting 543
51 Computational Identification of Related Proteins:
BLAST, PSI BLAST, and Other Tools
Qunfeng Dong and Volker Brendel 555
52 Protein Identification and Analysis Tools on the ExPASy Server
Elisabeth Gasteiger, Christine Hoogland, Alexandre Gattiker,
Severine Duvaud, Marc R. Wilkins, Ron D. Appel, and Amos Bairoch 571
53 Protein Sequence Databases
Michele Magrane, Maria Jesus Martin, Claire O Donovan,
and Rolf Apweiler 609
54 In Silico Characterization of Proteins: InterPro and Proteome Analysis
Nicola Jane Mulder, Manuela Pruess, and Rolf Apweiler 619
55 Computational Prediction of Protein Protein Interactions
Anton J. Enright, Lucy Skrabanek, and Gary D. Bader 629
56 The Yeast Two Hybrid System for Detecting Interacting Proteins
llya G. Serebriiskii, Erica A. Golemis, and Peter Uetz 653
57 Antibody Affinity Purification to Detect Interacting Proteins
Sonia Navarro and Lucio Comai 683
58 Biomolecular Interaction Analysis Coupled With Mass Spectrometry
to Detect Interacting Proteins
Setsuko Hashimoto, Toshiaki Isobe, and Tohru Natsume 689
59 Assessment of Antibody Antigen Interaction Using SELDI Technology
Li Shan Hsieh, Ramy Moharram, Emilia Caputo, and Brian M. Martin 699
60 Protein and Peptide Microarray Based Assay Technology
Scott T. Clarke 709
61 Production of Protein Microarrays Using Robotic Pin Printing Technologies
Ve Fang, Ann M. Ferrie, and Fang Lai 723
62 PCR Directed Protein In Situ Arrays
Joe Boutell and Mingyue He 735
63 Site Specific Immobilization of Proteins in a Microarray
Yee Peng R. Lue, Su Yin D. Yeo, Lay Pheng Tan, Grace Y. J. Chen,
andShao Q. Yao 743
64 A Guide to Protein Interaction Databases
Tiffany B. Fischer, Melissa Paczkowski, Michael F. Zettel, and Jerry Tsai 753
65 Deriving Function From Structure: Approaches and Limitations
Annabel E. Todd 801
66 Comparative Protein Structure Modeling
M. S. Madhusudhan, Marc A. Marti Renom, Narayanan Eswar, Bino John,
Ursula Pieper, Rachel Karchin, Min Yi Shen, and Andrej Sali 831
67 Classification of Protein Sequences and Structures
S. Rackovsky 861
Contents xi
68 How to Use Protein 1 D Structure Predicted by PROFphd
Burkhard Rost 875
69 Classification of Protein Folds
Robert B. Russell 903
70 Protein Threading
Andrew E. Torda 921
71 High Throughput Crystallography for Structural Proteomics
Jeff Yon, Mladen Vinkovic, and Harren Jhoti 939
72 Automated High Throughput Protein Crystallization
Arezou Azarani 955
73 NMR Based Structure Determination of Proteins in Solution
Andrzej Ejchart and Igor Zhukov 967
Index 983
|
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| spelling | The proteomics protocols handbook ed. by John M. Walker Totowa, N.J. Humana Press 2005 XVIII, 988 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Eiwitsynthese gtt Eiwitten gtt Protéomique Transcriptiefactoren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Proteomics Handbooks, manuals, etc Proteomics methods Proteomanalyse (DE-588)4596545-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteomanalyse (DE-588)4596545-6 s b DE-604 Walker, John M. Sonstige oth Erscheint auch als Online-Ausgabe 1-58829-343-2 http://www.loc.gov/catdir/toc/ecip0420/2004016126.html Table of contents HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013193767&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The proteomics protocols handbook Eiwitsynthese gtt Eiwitten gtt Protéomique Transcriptiefactoren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Proteomics Handbooks, manuals, etc Proteomics methods Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4596545-6 (DE-588)4143413-4 |
| title | The proteomics protocols handbook |
| title_auth | The proteomics protocols handbook |
| title_exact_search | The proteomics protocols handbook |
| title_full | The proteomics protocols handbook ed. by John M. Walker |
| title_fullStr | The proteomics protocols handbook ed. by John M. Walker |
| title_full_unstemmed | The proteomics protocols handbook ed. by John M. Walker |
| title_short | The proteomics protocols handbook |
| title_sort | the proteomics protocols handbook |
| topic | Eiwitsynthese gtt Eiwitten gtt Protéomique Transcriptiefactoren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Proteomics Handbooks, manuals, etc Proteomics methods Proteomanalyse (DE-588)4596545-6 gnd |
| topic_facet | Eiwitsynthese Eiwitten Protéomique Transcriptiefactoren Wetenschappelijke technieken Wissenschaftliches Arbeiten Proteomics Handbooks, manuals, etc Proteomics methods Proteomanalyse Aufsatzsammlung |
| url | http://www.loc.gov/catdir/toc/ecip0420/2004016126.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013193767&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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