Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells:
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| Format: | Thesis Microfilm Book |
| Language: | English |
| Published: |
2003
|
| Edition: | Mikrofiche-Ausg. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 78 Bl. Ill., graph. Darst. |
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MARC
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| 100 | 1 | |a Liu, Jian |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |c by Jian Liu |
| 250 | |a Mikrofiche-Ausg. | ||
| 264 | 1 | |c 2003 | |
| 300 | |a 78 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |a Rostock, Univ., Diss., 2004. - Mikrofiche-Ausg.: 1 Mikrofiche : 24x | ||
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|---|---|
| adam_text |
TABLE
OF
CONTENTS
1.
INTRODUCTION
1
1.1.
CANCER:
GENERAL
REMARKS
1
1.2.
COAGULATION,
THROMBOSIS,
AND
MALIGNANCY
1
1.3.
CLOTTING
AND
CELLULAR
(POSTCLOTTING)
ACTIVITIES
OF
THROMBIN
2
1.4.
THROMBIN
RECEPTORS
AND
BINDING
MOLECULES
3
1.4.1.
PROTEASE-ACTIVATED
RECEPTORS
3
1.4.1.1.
THE
PAR
FAMILY
AND
THEIR
UNIQUE
MECHANISM
OF
PROTEOLYTIC
ACTIVATION
3
1.4.1.2.
EXPRESSION
OF
THE
THROMBIN
RECEPTORS
PAR-1
,
-3,
AND
-4
5
1.4.2.
ADDITIONAL
THROMBIN-BINDING
SITES
9
1.4.2.1.
THROMBOMODULIN
(TM)
9
1.4.2.2.
GLYCOPROTEIN
IBA
(GPIBA)
9
1.5.
CURRENT
CONCEPTS
OF
THE
ASSOCIATION
BETWEEN
THROMBIN,
PARS,
AND
MALIGNANCY
10
1.6.
THROMBIN,
THROMBIN
RECEPTORS,
AND
PROSTATE
CANCER
12
1.7.
AIMS
OF
THE
PRESENT
INVESTIGATION
13
2.
MATERIALS
AND
METHODS
15
2.1.
CELL
CULTURE
15
2.2.
RNA
ISOLATION
AND
REVERSE
TRANSCRIPTION-PCR
15
2.3.
FLOW
CYTOMETRY
16
2.4.
THROMBIN
CLOTTING
TIME
17
2.5.
THROMBIN
AMIDOLYTIC
ACTIVITY
17
2.6.
PROLIFERATION
ASSAY
18
2.7.
DNA
FRAGMENTATION
DETECTION
18
2.8.
CELL
CYCLE
ANALYSIS
19
2.9.
CELL
MIGRATION
19
2.10.
ADHESION
ASSAY
19
2.11.
IMMUNOASSAY
(ELISA)
20
2.12.
STATISTICS
20
3.
RESULTS
21
3.1.
EXPRESSION
PROFILE
OF
THROMBIN
RECEPTORS
AND
THROMBIN-ASSOCIATED
MOLECULES
21
3.1.1.
EXPRESSION
OF
PROTEASE-ACTIVATED
RECEPTORS
21
3.1.2.
EXPRESSION
OF
ADDITIONAL
THROMBIN-ASSOCIATED
MOLECULES
23
3.2.
INHIBITION
OF
THROMBIN
ACTIVITY
25
3.2.1.
D-PHE-PRO-ARG
CHLOROMETHYL
KETONE
(PPACK)
25
3.2.2.
THROMBIN-SPECIFIC
APTAMER
25
3.3.
EFFECTS
OF
THROMBIN
ON
CELL
PROLIFERATION
27
3.4.
EFFECTS
OF
THROMBIN
ON
APOPTOSIS
AND
CELL
CYCLE
DISTRIBUTION
31
3.5.
EFFECTS
OF
THROMBIN
ON
CELL
MIGRATION
33
3.6.
EFFECTS
OF
THROMBIN
ON
CELL
ADHESION
TO
EXTRACELLULAR
MATRIX
(ECM)
PROTEINS
36
3.6.1.
EFFECTS
OF
SHORT-TIME
THROMBIN
TREATMENT
ON
CELL
ADHESION
TO
ECM
PROTEINS
36
3.6.2.
EFFECTS
OF
PROLONGED
THROMBIN
PRETREATMENT
ON
CELL
ADHESION
TO
ECM
PROTEINS
39
3.7.
EFFECTS
OF
THROMBIN
ON
SECRETION
OF
GROWTH/ANGIOGENIC
FACTORS
39
3.7.1.
VASCULAR
ENDOTHELIAL
GROWTH
FACTOR
(VEGF)
39
3.7.2.
INTERLEUKIN-8
(IL-8)
41
3.7.3.
INTERLEUKIN-6
(IL-6)
43
4.
DISCUSSION
45
4.1.
EXPRESSION
OF
THROMBIN
RECEPTORS
AND
THROMBIN-ASSOCIATED
MOLECULES
45
4.1.1.
EXPRESSION
OF
A
MONOFUNCTIONAL
THROMBIN
RECEPTOR
SYSTEM
IN
PROSTATE-DERIVED
CELLS
45
4.1.2.
ABSENT
EXPRESSION
OF
TM
AND
RESTRICTED
EXPRESSION
OF
GPIBA
46
4.2.
THROMBIN
MODULATES
THE
PHENOTYPE
OF
PROSTATE
CANCER
CELLS
THROUGH
PAR-1
47
4.2.1.
DOSE-DEPENDENT
MODULATING
EFFECTS
OF
THROMBIN
ON
CELL
PROLIFERATION
47
4.2.2.
STIMULATION
OF
SECRETION
OF
GROWTH/ANGIOGENIC
FACTORS
BY
THROMBIN
50
4.2.3.
THROMBIN
MODULATES
MIGRATION
AND
ADHESION
OF
PROSTATE
CANCER
CELLS
53
4.3.
LOCAL
GENERATION
OF
THROMBIN
IN
TUMOR
TISSUE
55
4.4.
QUESTIONS
AND
OUTLOOK
57
5.
SUMMARY
6.
REFERENCES
58
62
ACKNOWLEDGEMENTS
GUARANTEE |
| any_adam_object | 1 |
| author | Liu, Jian |
| author_facet | Liu, Jian |
| author_role | aut |
| author_sort | Liu, Jian |
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| building | Verbundindex |
| bvnumber | BV019804455 |
| ctrlnum | (OCoLC)634041676 (DE-599)BVBBV019804455 |
| discipline | Medizin |
| edition | Mikrofiche-Ausg. |
| format | Thesis Microfilm Book |
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| spelling | Liu, Jian Verfasser aut Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells by Jian Liu Mikrofiche-Ausg. 2003 78 Bl. Ill., graph. Darst. txt rdacontent h rdamedia he rdacarrier Rostock, Univ., Diss., 2004. - Mikrofiche-Ausg.: 1 Mikrofiche : 24x (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013129977&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Liu, Jian Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| title_auth | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| title_exact_search | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| title_full | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells by Jian Liu |
| title_fullStr | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells by Jian Liu |
| title_full_unstemmed | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells by Jian Liu |
| title_short | Molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| title_sort | molecular and cellular consequences of thrombin receptor expression in malignant prostate cells |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013129977&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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