Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2003
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Enth. 5 Sonderabdr. aus verschiedenen Zeitschr. - Bremen, Univ., Diss., 2003 |
| Physical Description: | 159 S. Ill., graph. Darst. : 24 cm |
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| 300 | |a 159 S. |b Ill., graph. Darst. : 24 cm | ||
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Record in the Search Index
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|---|---|
| adam_text |
CONTENTS
SUMMARY
.
1
ZUSAMMENFASSUNG
.
2
PARTI:
COMBINED
PRESENTATION
OF
RESULTS
A
INTRODUCTION
.
6
1
RIBOSOMAL
RNA-TARGETED
OLIGONUCLEOTIDE
PROBES
FOR
INVESTIGATION
OF
MICROORGANISMS
.
6
2
LIMITATIONS
OF
RRNA-TARGETED
PROBES
AND
METHODOLOGICAL
IMPROVEMENTS
TO
OVERCOME
THEM
.
8
2.1
THE
TARGET
MOLECULE
.
8
2.2
CELLULAR
RIBOSOME
CONTENT
.
9
2.3
POLYRIBONUCLEOTIDE
PROBES
.
10
2.4
PEPTIDE
NUCLEIC
ACIDS
.
10
2.5
ENZYMATIC
SIGNAL
AMPLIFICATION
.
11
2.6
SELF-LIGATING
PROBES
.
11
2.7
NUCLEOTIDE-SPECIFIC
QUENCHING
OF
FLUORESCENT
DYES
.
12
2.8
ACCESSIBILITY
OF
PROBE
TARGET
SITES
.
13
2.9
AUTOMATION
.
14
3
LINKING
FISH
TO
FUNCTION
AND
ACTIVITY
.
16
4
OUTLINE
OF
THE
PRESENT
STUDY
.
18
B
RESULTS
AND
DISCUSSION
.
21
1
IN
SITU
ACCESSIBILITY
OF
SMALL-SUBUNIT
RRNA
TO
CY3-LABELED
OLIGONUCLEOTIDE
PROBES
.
21
2
EFFECTS
OF
THE
3D-STRUCTURE
OF
THE
SMALL
RIBOSOMAL
SUBUNIT
ON
FLUORESCENCE
IN
SITU
HYBRIDIZATION
.
30
3
NUCLEOBASE-SPECIFIC QUENCHING
OF
FLUORESCENCE
IN
SITU
HYBRIDIZATION
.
34
4
TOWARD
MORE
RATIONAL
PROBE
DESIGN
.
40
C
REFERENCES
.
43
PART
2:
PUBLICATIONS
A
LIST
OF
PUBLICATIONS
.
58
B
PUBLICATIONS
.
59
1
IN
SITU
ACCESSIBILITY
OF
SMALL-SUBUNIT
RRNA
OF
MEMBERS
OF
THE
DOMAINS
BACTERIA,
ARCHAEA,
AND
EUCARYA
TO
CY3-LABELED
OLIGONUCLEOTIDE
PROBES
.
59
2
IN
SITU
ACCESSIBILITY
OF
SACCHAROMYCES
CEREVISIAE 26S
RRNA TO
CY3-LABELED
'
OLIGONUCLEOTIDE
PROBES
COMPRISING
THE
D1/D2
DOMAINS
.
71
3
IS
THE
IN
SITU
ACCESSIBILITY
OF
THE
16S
RRNA
OF
ESCHERICHIA
EDITOR
CY3-LABELED
OLIGONUCLEOTIDE
PROBES
PREDICTED
BY
A
3D-STRUCTURE
MODEL
OF
THE
30S
RIBOSOMAL
SUBUNIT?
.
89
4
NUCLEOBASE-SPECIFIC
QUENCHING
EFFECTS
ON
FLUORESCENCE
IN
SITU
HYBRIDIZATION
WITH
RRNA-TARGETED
OLIGONUCLEOTIDE
PROBES
.
109
5
THE
IDENTIFICATION
OF
MICROORGANISMS
BY
FLUORESCENCE
IN
SITU
HYBRIDIZATION
.
133
PART
3:
APPENDIX
A
LIST
OF
OLIGONUCLEOTIDE
PROBES
.
141
DANKSAGUNG
.
159 |
| any_adam_object | 1 |
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| author_facet | Behrens, Sebastian |
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| dewey-full | 570 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570 |
| dewey-search | 570 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-08-16T01:01:07Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-013121097 |
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| physical | 159 S. Ill., graph. Darst. : 24 cm |
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| spelling | Behrens, Sebastian Verfasser aut Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms vorgelegt von Sebastian Behrens 2003 159 S. Ill., graph. Darst. : 24 cm txt rdacontent n rdamedia nc rdacarrier Enth. 5 Sonderabdr. aus verschiedenen Zeitschr. - Bremen, Univ., Diss., 2003 Bremen, Univ., Diss., 2003 Mikroorganismus - Bestimmung - Methode Mikroorganismus - Identifikation - Fluoreszenz-in-situ-Hybridisierung Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Fluoreszenz-in-situ-Hybridisierung (DE-588)4528641-3 gnd rswk-swf Identifikation (DE-588)4072712-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mikroorganismus (DE-588)4039226-0 s Identifikation (DE-588)4072712-9 s Fluoreszenz-in-situ-Hybridisierung (DE-588)4528641-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013121097&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Behrens, Sebastian Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms Mikroorganismus - Bestimmung - Methode Mikroorganismus - Identifikation - Fluoreszenz-in-situ-Hybridisierung Mikroorganismus (DE-588)4039226-0 gnd Fluoreszenz-in-situ-Hybridisierung (DE-588)4528641-3 gnd Identifikation (DE-588)4072712-9 gnd |
| subject_GND | (DE-588)4039226-0 (DE-588)4528641-3 (DE-588)4072712-9 (DE-588)4113937-9 |
| title | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms |
| title_auth | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms |
| title_exact_search | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms |
| title_full | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms vorgelegt von Sebastian Behrens |
| title_fullStr | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms vorgelegt von Sebastian Behrens |
| title_full_unstemmed | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms vorgelegt von Sebastian Behrens |
| title_short | Methodological aspects of fluorescence in situ hybridization for the identification of microorganisms |
| title_sort | methodological aspects of fluorescence in situ hybridization for the identification of microorganisms |
| topic | Mikroorganismus - Bestimmung - Methode Mikroorganismus - Identifikation - Fluoreszenz-in-situ-Hybridisierung Mikroorganismus (DE-588)4039226-0 gnd Fluoreszenz-in-situ-Hybridisierung (DE-588)4528641-3 gnd Identifikation (DE-588)4072712-9 gnd |
| topic_facet | Mikroorganismus - Bestimmung - Methode Mikroorganismus - Identifikation - Fluoreszenz-in-situ-Hybridisierung Mikroorganismus Fluoreszenz-in-situ-Hybridisierung Identifikation Hochschulschrift |
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