Functional analysis of porins of Mycobacterium smegmatis in vivo:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Erlangen-Nürnberg, Univ., 2004. - Enth. dt. Zsfassung. |
| Beschreibung: | 111 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | Index
INDEX
1 Zusammenfassung 1
2 Summary 2
3 Introduction 3
3.1 The genus Mycobacterium 3
3.1.1 Taxonomy 3
3.1.2 Medical relevance of mycobacteria 3
3.2 The architecture of the mycobacterial outer membrane 4
3.3 Porins of Gram negative bacteria 5
3.4 Functions of porins in pathogenicity 6
3.5 Mycobacterial porins 8
3.5.1 Porins in slow growing mycobacteria 8
3.5.2 Porins of M. smegmatis 9
3.6 Homologous recombination in mycobacteria 10
4 Goals of this study 12
5 results / publications 13
5.1 Establishment of the Flp / FRT recombination System in M. smegmatis 13
5.2 Construction of porin gene deletion mutants of M. smegmatis 24
5.3 Porin gene expression in M. smegmatis 32
53 1 Improvement of RNA preparations from mycobacteria 33
5.3.2 Expression of msp porin genes in msp deletion mutants .. . 36
5.3.3 Regulation of expression of silent porin genes in M smegmatis 38
5.4 Characterization of a mspA deletion mutant of M smegmatis 39
5.4.1 Determination of the partition coefficients of antibiotics 4G
5.4.2 Resistance of a mspA mutant towards antimicrobial agents 42
5.4.3 Permeability of M. smegmatis to amino acids 50
5.4 4 Characterization of Single porin deletion mutants of M smegmatis. 52
5.5 Characterization of a double porin mutant strain of h/l. smegmatis 55
5.5.1 The permeability properties are drasticaliy altered in a mspA mspC mutant
of M. smegmatis . 56
Index
5.5.2 Deletion of the main porin genes confers high level resistance to various
antimicrobial agents 71
5.6 Stable Integration of mycgfp2+ in M. smegmatis via attB I attP 79
5.7 Further publications 82
6 DlSCUSSION 83
6.1 Site specific recombination in M. smegmatis provided by the Flp / FRT
recombination system 83
6.2 Deletion of porin genes in M. smegmatis 84
6.3 Regulation of porin gene expression in M. smegmatis 86
6.4 MspA plays the major role in the cell wall permeability of M. smegmatis 88
6.5 Consecutive deletion of mspA and mspC results in high level resistance of
M. smegmatis to multiple drugs 89
6.6 Stable expression of gfp in M. smegmatis 90
6.7 Conclusion and perspectives 91
6.8 Autnors contributions 91
7 References 92
8 Appendix 102
8.1 Chemical structures of antibiotics and drugs 102
8.2 Antibiotic sensitivity measurements 103
8.2.1 M. smegmatis SMR5 (wild type) vs. MN01 (AmspA) 103
8.2.2 M. smegmatis SMR5 (wild type) vs. ML10 (AmspA , mspC) 104
8.3 Transport experiments 105
8.3.1 Uptake of amino acids by M. smegmatis 105
8.3.2 Concentration dependent uptake ratesfor M. smegmatis 106
8.3.2.1 Glucose 106
8.3.2.2 Leucine 107
8.3.2.3 Glutamic acid 108
8.3.2.4 Lysine 109
8.3.3 Uptake of chenodeoxycholate by M. smegmatis 110
8.4 Abbreviation index 110
|
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| author_GND | (DE-588)130002542 |
| author_facet | Stephan, Joachim 1977- |
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| indexdate | 2024-07-09T20:05:57Z |
| institution | BVB |
| language | English German |
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| spelling | Stephan, Joachim 1977- Verfasser (DE-588)130002542 aut Functional analysis of porins of Mycobacterium smegmatis in vivo von Joachim Stephan 2004 111 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., 2004. - Enth. dt. Zsfassung. Erlangen-Nürnberg, Univ., Diss., 2004 Mycobacterium smegmatis (DE-588)4607281-0 gnd rswk-swf Membran (DE-588)4038571-1 gnd rswk-swf Permeabilität (DE-588)4173828-7 gnd rswk-swf Porin (DE-588)4221584-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mycobacterium smegmatis (DE-588)4607281-0 s Porin (DE-588)4221584-5 s DE-604 Membran (DE-588)4038571-1 s Permeabilität (DE-588)4173828-7 s DE-188 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013106827&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stephan, Joachim 1977- Functional analysis of porins of Mycobacterium smegmatis in vivo Mycobacterium smegmatis (DE-588)4607281-0 gnd Membran (DE-588)4038571-1 gnd Permeabilität (DE-588)4173828-7 gnd Porin (DE-588)4221584-5 gnd |
| subject_GND | (DE-588)4607281-0 (DE-588)4038571-1 (DE-588)4173828-7 (DE-588)4221584-5 (DE-588)4113937-9 |
| title | Functional analysis of porins of Mycobacterium smegmatis in vivo |
| title_auth | Functional analysis of porins of Mycobacterium smegmatis in vivo |
| title_exact_search | Functional analysis of porins of Mycobacterium smegmatis in vivo |
| title_full | Functional analysis of porins of Mycobacterium smegmatis in vivo von Joachim Stephan |
| title_fullStr | Functional analysis of porins of Mycobacterium smegmatis in vivo von Joachim Stephan |
| title_full_unstemmed | Functional analysis of porins of Mycobacterium smegmatis in vivo von Joachim Stephan |
| title_short | Functional analysis of porins of Mycobacterium smegmatis in vivo |
| title_sort | functional analysis of porins of mycobacterium smegmatis in vivo |
| topic | Mycobacterium smegmatis (DE-588)4607281-0 gnd Membran (DE-588)4038571-1 gnd Permeabilität (DE-588)4173828-7 gnd Porin (DE-588)4221584-5 gnd |
| topic_facet | Mycobacterium smegmatis Membran Permeabilität Porin Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013106827&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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