Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium: = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | German English |
| Veröffentlicht: |
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 72 S. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium |b = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |c vorgelegt von Christian Pott |
| 246 | 1 | 1 | |a Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| 264 | 1 | |c 2004 | |
| 300 | |a 72 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 2004 | ||
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Datensatz im Suchindex
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| adam_text | Table of Contents
1 INTRODUCTION
1.1 Excitation-contraction-coupling in human myocardium 8
1.2 p-adrenergic stimulation in the human heart 9
1.3 The fc-adrenoceptor 11
1.3.1 Physiological and pathophysiological implications 11
1.3.2 Pharmacological properties of fS3-adrenoceptor agonists 12
1.3.3 eNOS-activation and the putative p3-adrenergic pathway 13
1.4 Aims of the study 14
2 MATERIALS AND METHODS
2.1 Material 16
2.1.1 Myocardial tissue 16
2.1.2 Reagents and antibodies 16
2.2 Functional Measurements 19
2.2.1 Measurements of isometric force of contraction 19
2.2.2 Simultaneous measurements of force and the intracellular
Ca2+-transient 19
2.2.3 Membrane preparation and binding experiments 21
2.3 Biochemical and immunohistochemical methods 22
2.3.1 Immunohistochemistry 22
2.3.2 Measurement of protein expression 24
2.3.3 Diaminofluorescein (DAF)-fluorometry 26
2.4 Statistical analysis 27
3 RESULTS
3.1 Protein expression of (53-adrenoceptors in human
non-failing right atrial and left ventricular myocardium 28
3.2 Inotropic effects of BRL 37344 in human non-failing 28
myocardium
3.2.1 Left ventricular myocardium 29
3.2.2 Right atrial myocardium 29
3.3.3 Influence of BRL 37344 on the intracellular Ca2+-transient 31
3.3 Agonism of BRL 37344 towards p,/2-adrenoceptors in right
Atrium 33
3.3.1 Affinity of BRL 37344 towards Pi-and p2-adrenoceptors 33
3.3.2 BRL 37344 in the presence of propranolol 34
3.4 BRL 37344-induced NO-liberation in human non-failing
myocardium 35
3.5 BRL 37344-induced cardiac activation of endothelial NO
synthase (eNOS) 37
3.5.1 eNOS-translocation 37
3.5.2 eNOS-phosphorylation 39
3.6 Modulation of inotropic effects by BRL 37344 **
3.6.1 Inotropic effects of BRL 37344 in presence of NO inhibition 44
3.6.2 Influence of BRL 37344 on isoprenaline induced inotropic effects 45
4 DISCUSSION AND CONCLUSIONS 46
4.1 Inotropic effects of the preferential p3-adrenoceptor
agonist BRL 37344 46
4.2 The fr-adrenergic signal transduction pathway 48
4.3 Physiological and pathophysiological implication of 51
Ps-adrenergic cardiac stimulation
4.4 Conclusions 53
5 SUMMARY 55
5 ZUSAMMENFASSUNG 56
6 REFERENCES 58
7 PREPUBLICATIONS 70
8 CURRICULUM VITAE 72
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| ctrlnum | (OCoLC)632995478 (DE-599)BVBBV019724323 |
| discipline | Medizin |
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| indexdate | 2024-07-09T20:04:40Z |
| institution | BVB |
| language | German English |
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| spelling | Pott, Christian 1976- Verfasser (DE-588)129707910 aut Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard vorgelegt von Christian Pott Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard 2004 72 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2004 Beta-Rezeptor (DE-588)4144967-8 gnd rswk-swf Herzmuskel (DE-588)4024675-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Herzmuskel (DE-588)4024675-9 s Beta-Rezeptor (DE-588)4144967-8 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013051392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pott, Christian 1976- Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard Beta-Rezeptor (DE-588)4144967-8 gnd Herzmuskel (DE-588)4024675-9 gnd |
| subject_GND | (DE-588)4144967-8 (DE-588)4024675-9 (DE-588)4113937-9 |
| title | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| title_alt | Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| title_auth | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| title_exact_search | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| title_full | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard vorgelegt von Christian Pott |
| title_fullStr | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard vorgelegt von Christian Pott |
| title_full_unstemmed | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard vorgelegt von Christian Pott |
| title_short | Functional, biochemical and immunohistochemical investigations on the beta 3-adrenoceptro in human myocardium |
| title_sort | functional biochemical and immunohistochemical investigations on the beta 3 adrenoceptro in human myocardium funktionelle biochemische und immunhistochemische untersuchungen zum beta 3 adrenozeptor am menschlichen myokard |
| title_sub | = Funktionelle, Biochemische und Immunhistochemische Untersuchungen zum beta 3-Adrenozeptor am menschlichen Myokard |
| topic | Beta-Rezeptor (DE-588)4144967-8 gnd Herzmuskel (DE-588)4024675-9 gnd |
| topic_facet | Beta-Rezeptor Herzmuskel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013051392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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