Comparative studies on manufacturing methods for cationic liposomes:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Aachen
Shaker
2004
|
| Edition: | 1. Aufl. |
| Series: | Berichte aus der Pharmazie
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zugl.: München, Univ., Diss., 2003 |
| Physical Description: | XVIII, 199 S. Ill., graph. Darst. 210 mm x 148 mm, 333 gr. |
| ISBN: | 3832230084 |
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Record in the Search Index
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| adam_text | BERICHTE AUS DER PHARMAZIE
SASKIAWAJBLER
COMPARATIVE STUDIES
ON MANUFACTURING METHODS
FOR CATIONIC LIPOSOMES
SHAKER VERLAG
AACHEN
2004
CONTENT
I INTRODUCTION 1
1.1 LIPOSOMES 1
1.1.1 STRUCTURE AND CLASSIFICATION OF LIPOSOMES 4
1.1.1.1 LIPOSOME CLASSIFICATION 7
1.1.1.2 LIPOSOME CHARACTERIZATION 9
1.1.2 PREPARATION TECHNIQUES 12
1.1.3 FIELDS OF APPLICATION 18
1.1.4 CATIONIC LIPOSOMES 21
1.1.4.1 ENDOTHELIAL TARGETING 23
1.1.4.2 GENE THERAPY 25
1.2 AIM / OBJECTIVES OF THIS WORK 27
II MATERIALS AND METHODS 29
11.1 MATERIALS USED 29
11.1.1 LIPIDS 29
11.1.2 ACTIVE COMPOUNDS AND FLUORESCENT DYES 30
11.1.3 OTHER MATERIALS 31
11.2 PREPARATION OF LIPOSOMES 33
11.2.1 PREPARATION OF MLV DISPERSIONS 33
II.2.1.1 PREPARATION OF MLVS USING THE FILM-METHOD 33
H.2.1.2 PREPARATION OF MLVS USING THE LIPID-DISPERSION-METHOD 33
11.2.2 PREPARATION OF LIPOSOMES USING HIGH-PRESSURE DEVICES 33
11.2.2.1 APV GAULIN MICRON LAB 40* 33
11.2.2.2 AVESTIN EMULSIFLEX* 35
XI!
CONTENT
PREPARATION OF LIPOSOMES USING FILTER EXTRUDER DEVICES 36
10 ML THERMOBARREL EXTRUDER - LIPEX* INC 36
100 ML THERMOBARREL EXTRUDER - LIPEX* INC 37
STERILE FILTRATION UNIT - SARTORIUS* 38
ETHANOL-INJECTION-TECHNIQUE 38
PREPARATION OF LIPOSOMES BY SONICATION 39
BRANSON* SONIFIER 39
ULTRASONIC BATH 39
PREPARATION OF LIPOSOMES USING THE LIPID-LOADED-MEMBRANE-
METHOD ./. 39
PREPARATION OF LIPOSOMES USING THE LIPID-EXTRUDER 40
LYOPHILISATION OF LIPOSOMES 41
STERILIZATION OF LIPOSOMES 42
HIGH-PRESSURE STERILIZATION 42
STERILE-FILTRATION 43
PREPARATION OF DYE-LABELED LIPIDS 44
ANALYTICAL METHODS 44
DYNAMIC LASER LIGHT SCATTERING (= PHOTON CORRELATION
SPECTROSCOPY) 44
ZETAPOTENTIAL MEASUREMENTS 45
GAS CHROMATOGRAPHY 45
FLUORESCENCE RESONANCE ENERGY TRANSFER 46
SCANNING ELECTRON MICROSCOPY ...*. 47
LIGHT OPTICAL MICROSCOPE 47
ENZYMATIC DETERMINATION OF THE LIPID CONTENT 47
HIGH PRESSURE LIQUID CHROMATOGRAPHY 48
II.2.3
II.2.3.1
II.2.3.2
II.2.3.3
II.2.4
II.2.5
II.2.5.1
II.2.5.2
II.2.6
II.2.7
II.2.8
II.2.9
II.2.9.1
II.2.9.2
II.2.10
II.3
II.3.1
II.3.2
II.3.3
II.3.4
II.3.5
II.3.6
II.3.7
II.3.8
CONTENT . XIII
II.3.9 DIFFERENTIAL SCANNING CALORIMETRY 49
III RESULTS AND DISCUSSION 51
III.L LIPID-DISPERSION-METHOD 51
111.1.1 PREPARATION OF LIPOSOMES USING HIGH-PRESSURE DEVICES 51
111.1.1.1 APV GAULIN MICRON LAB 40* 52
111.1.1.1.1 LIPOSOME CHARACTERIZATION * 53
111.1.1.1.2 ASSESSMENT OF FORMULATION PARAMETERS ON LIPOSOME PROPERTIES
55
111.1.1.1.3 SCALE-UP 58
111.1.1.1.4 SUMMARY 59
III.L. 1.1.5 STERILE FILTRATION 60
111.1.1.1.6 RESULTS AND DISCUSSION 61
111.1.1.1.7 SUMMARY 63
111.1.1.2 AVESTIN EMULSIFLEX C5* 64
111.1.1.2.1 HIGH-PRESSURE HOMOGENIZATION USING THE AVESTIN EMULSIFLEX
C5* 64
111.1.1.2.2 HIGH-PRESSURE HOMOGENIZATION PLUS CONSECUTIVE EXTRUSION
USING
THE AVESTIN EMULSIFLEX C5* 66
111.1.1.2.3 LIPOSOME EXTRUSION USING THE AVESTIN EMULSIFLEX C5* 68
111.1.1.2.4 RESULTS AND DISCUSSION 68
111.1.1.2.5 SUMMARY 69
111.1.2 PREPARATION OF LIPOSOMES USING FILTER EXTRUDER DEVICES 70
III.1.2.1 THERMOBARREL EXTRUDER - LIPEX* INC 70
111.1.2.1.1 FEASIBILITY STUDIES USING THE 10 ML EXTRUDER; LIPOSOME
CHARACTERIZATION 70
111.1.2.1.2 LIPOSOME CHARACTERIZATION VIA ZETAPOTENTIAL MEASUREMENTS 73
111.1.2.1.3 ASSESSMENT OF THE INFLUENCE OF EXTRUSION TEMPERATURE 75
XIV
CONTENT
ASSESSMENT OF USING STERILE FILTRATION MEMBRANES FOR LIPOSOME
EXTRUSION 5
ASSESSMENT OF INFLUENCE OF LIPID CONCENTRATION ON EXTRUSION RESULTS 77
MONITORING THE LIPID CONTENTS DURING MANUFACTURING PROCESSES 78
SUMMARY
7
9
SCALE-UP STUDIES 80
100 ML THERMOBARREL EXTRUDER - LIPEX* INC 80
1000 ML STERILE-FILTRATION UNIT - SARTORIUS* 82
SUMMARY 86
MEMBRANE STUDIES *** 86
POLYCARBONATE - MEMBRANES 88
RESULTS AND DISUSSION 88
SUMMARY 93
DETERMINATION OF RESIDUAL SOLVENTS 94
INFLUENCE OF EVAPORATION TIME ON SOLVENT TRACES 94
RESULTS AND DISCUSSION 95
PREPARATION OF LIPOSOMES BY SONICATION 97
BRANSON* SONIFIER (ULTRASONIC TIP) 97
ULTRASONIC BATH 98
RESULTS AND DISCUSSION 98
SUMMARY 100
ETHANOL-INJECTION-TECHNIQUE * 101
RESULTS AND DISCUSSION 102
SUMMARY 1 4
III.1.2.1.4
III.1.2.1.5
III.1.2.1.6
III.1.2.1.7
III.1.2.2
III.L. 2.2.1
III.L. 2.2.2
III.1.2.2.3
111.1.3
111.1.3.1
III.1.3.1.1
III.L.3.1.2
111.1.4
III.1.4.1
111.1.4.2
III.2
III.2.1
III.2.2
III.2.3
III.2.4
III.3
III.3.1
III.3.2
CONTENT XV
111.4 THE LIPID-EXTRUDER 105
111.4.1 DEVELOPMENT OF THE DEVICE 106
111.4.2 RESULTS AND DISCUSSION 106
111.4.3 LIPOSOME CHARACTERIZATION 107
111.4.4 FORMULATION STUDIES 110
111.4.5 SCALE-UP 118
111.4.6 ENCAPSULATION STUDIES 119
111.4.7 DETERMINATION OF RESIDUAL SOLVENTS 121
111.4.8 SUMMARY 122
111.5 THE LIPID-LOADED-MEMBRANE-METHOD 124
111.5.1 RESULTS AND DISCUSSION 125
111.5.2 SCALE-UP 126
111.5.3 MEMBRANE STUDIES 129
111.5.4 SUMMARY 130
111.6 LYOPHILIZATION 131
111.6.1 RESULTS AND DISCUSSION 132
111.6.2 SUMMARY 134
111.7 STERILIZATION OF LIPOSOMES 135
111.7.1 HIGH-PRESSURE STERILIZATION 135
111.7.1.1 RESULTS AND DISCUSSION 136
111.7.1.2 SUMMARY 141
111.7.2 STERILE-FILTRATION 141
III.7.2.1 RESULTS AND DISCUSSION 141
HI.7.2.2 SUMMARY 1142
XVI CONTENT
III.8 FRET EXPERIMENTS 143
111.8.1 ASSESSMENT OF THE INFLUENCE OF DIFFERENT PROCESS TECHNOLOGIES
FOR
LIPOSOME PREPARATION ON FRET 143
HI.8.1.1 INVESTIGATION OF HIGH-PRESSURE-HOMOGENIZATION AND FRET 149
111.8.2 GENERAL FACTORS AFFECTING FRET IN LIPOSOMES 153
111.8.2.1 INFLUENCE OF LIPOSOME SIZE ON FRET 153
111.8.2.2 INFLUENCE OF MEMBRANE FLUIDITY ON FRET 157
111.8.2.3 INFLUENCE OF LIPOSOME SURFACE CHARGE ON FRET 160
111.8.2.4 INFLUENCE OF LYOPHILIZATION OF LIPOSOMES ON FRET 164
111.8.2.5 INFLUENCE OF LONG-TERM STORAGE OF LIPOSOMES ON FRET 169
111.8.2.6 VESICLE FUSION EXPERIMENTS 173
IV CONCLUSIONS AND FINAL SUMMARY 179
REFERENCES
:
I
85
CURRICULUM VITAE 201
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| indexdate | 2024-07-09T20:00:35Z |
| institution | BVB |
| isbn | 3832230084 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-012910182 |
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| physical | XVIII, 199 S. Ill., graph. Darst. 210 mm x 148 mm, 333 gr. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
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| publisher | Shaker |
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| spelling | Waibler, Saskia Verfasser aut Comparative studies on manufacturing methods for cationic liposomes Saskia Waibler 1. Aufl. Aachen Shaker 2004 XVIII, 199 S. Ill., graph. Darst. 210 mm x 148 mm, 333 gr. txt rdacontent n rdamedia nc rdacarrier Berichte aus der Pharmazie Zugl.: München, Univ., Diss., 2003 Herstellung (DE-588)4159653-5 gnd rswk-swf Liposom (DE-588)4122262-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Liposom (DE-588)4122262-3 s Herstellung (DE-588)4159653-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012910182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Waibler, Saskia Comparative studies on manufacturing methods for cationic liposomes Herstellung (DE-588)4159653-5 gnd Liposom (DE-588)4122262-3 gnd |
| subject_GND | (DE-588)4159653-5 (DE-588)4122262-3 (DE-588)4113937-9 |
| title | Comparative studies on manufacturing methods for cationic liposomes |
| title_auth | Comparative studies on manufacturing methods for cationic liposomes |
| title_exact_search | Comparative studies on manufacturing methods for cationic liposomes |
| title_full | Comparative studies on manufacturing methods for cationic liposomes Saskia Waibler |
| title_fullStr | Comparative studies on manufacturing methods for cationic liposomes Saskia Waibler |
| title_full_unstemmed | Comparative studies on manufacturing methods for cationic liposomes Saskia Waibler |
| title_short | Comparative studies on manufacturing methods for cationic liposomes |
| title_sort | comparative studies on manufacturing methods for cationic liposomes |
| topic | Herstellung (DE-588)4159653-5 gnd Liposom (DE-588)4122262-3 gnd |
| topic_facet | Herstellung Liposom Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012910182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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