Vaccinia virus and poxvirology: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2004
|
| Series: | Methods in molecular biology
269 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XIX, 396 S. Ill., graph. Darst. |
| ISBN: | 1588292290 |
Staff View
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| 245 | 1 | 0 | |a Vaccinia virus and poxvirology |b methods and protocols |c edited by Stuart N. Isaacs ; foreword by Bernard Moss |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2004 | |
| 300 | |a XIX, 396 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 269 | |
| 500 | |a Includes bibliographical references and index | ||
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| 650 | 7 | |a Vaccinia virus |2 cabt | |
| 650 | 4 | |a Poxvirus recombinants - Manuels de laboratoire | |
| 650 | 4 | |a Vaccine - Manuels de laboratoire | |
| 650 | 4 | |a DNA Replication | |
| 650 | 4 | |a Molecular Biology |x methods | |
| 650 | 4 | |a Poxviridae |x genetics | |
| 650 | 4 | |a Recombinant poxviruses |v Laboratory manuals | |
| 650 | 4 | |a Vaccinia virus |x genetics | |
| 650 | 4 | |a Vaccinia virus |x immunology | |
| 650 | 4 | |a Vaccinia |v Laboratory manuals | |
| 650 | 4 | |a Virus Replication | |
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Record in the Search Index
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Contents
Foreword v
Preface vii
Acknowledgments viii
Contributors xiii
Corresponding Authors xvii
1 Working Safely with Vaccinia Virus: Laboratory Technique
and the Role of Vaccinia Vaccination
Stuart N. Isaacs 1
2 Construction and Isolation of Recombinant Vaccinia Virus
Using Genetic Markers
Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco 15
3 Construction of Recombinant Vaccinia Virus:
Cloning into the Thymidine Kinase Locus
Chelsea M. Byrd and Dennis E. Hruby 31
4 Transient and Inducible Expression of VacciniaAT7 Recombinant
Viruses
Mohamed Ragaa Mohamed and Edward G. Niles 41
5 Construction of Recombinant Vaccinia Viruses Using
Leporipoxvirus Catalyzed Recombination and
Reactivation of Orthopoxvirus DNA
Xiao Dan Yao and David H. Evans 51
6 Construction of cDNA Libraries in Vaccinia Virus
Ernest S. Smith, Shuying Shi, and Maurice Zauderer 65
7 Construction and Isolation of Recombinant MVA
Caroline Staib, Ingo Drexler, and Gerd Sutter 77
8 Growing Poxviruses and Determining Virus Titer
Girish ). Kotwal and Melissa Rose Abrahams 101
9 Rapid Preparation of Vaccinia Virus DNA Template for Analysis
and Cloning by PCR
Rachel L. Roper 713
ix
x Contents
10 Orthopoxvirus Diagnostics
Hermann Meyer, Inger K. Damon, and Joseph J. Esposito 119
11 An In Vitro Transcription System for Studying Vaccinia
Virus Early Genes
Steven S. Broyles and Marcia Kremer 135
12 An In Vitro Transcription System for Studying Vaccinia Virus
Late Genes
Cynthia F. Wright 143
13 Studying Vaccinia Virus RNA Processing In Vitro
Paul D. Gershon 151
14 Methods for Analysis of Poxvirus DNA Replication
Paula Traktman and Kathleen Boyle 169
15 Studying the Binding and Entry of the Intracellular and
Extracellular Enveloped Forms of Vaccinia Virus
Mansun Law and Geoffrey L. Smith 187
16 Pox, Dyes, and Videotape:
Making Movies of GFP Labeled Vaccinia Virus
Brian M. Ward 205
1 7 Interaction Analysis of Viral Cytokine Binding Proteins
Using Surface Plasmon Resonance
Bruce T. Seet and Grant McFadden 219
1 8 Monitoring of Human Immunological Responses to Vaccinia Virus
Richard Harrop, Matthew G. Ryan, Hana Golding,
Irina Redchenko, and Miles W. Carroll 243
19 Vaccinia Virus as a Tool for Immunologic Studies
Nia Tatsis, Gomathinayagam Sinnathamby,
and Laurence C. Eisenlohr 267
20 Mouse Models for Studying Orthopoxvirus Respiratory Infections
fill Schriewer, R. Mark L. Buller, and Gelita Owens 289
21 Viral Glycoprotein Mediated Cell Fusion Assays Using
Vaccinia Virus Vectors
Katharine N. Bossart and Christopher C. Broder 309
Contents xi
22 Use of Dual Recombinant Vaccinia Virus Vectors to Assay
Viral Glycoprotein Mediated Fusion with Transfection Resistant
Primary Cell Targets
Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman 333
23 Poxvirus Bioinformatics
Chris Upton 347
24 Preparation and Use of Molluscum Contagiosum Virus
from Human Tissue Biopsy Specimens
Nadja V. Melquiot and Joachim J. Bugert 371
Index 385 |
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| dewey-full | 579.2/432 579.2432 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
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| dewey-search | 579.2/432 579.2432 |
| dewey-sort | 3579.2 3432 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| spelling | Vaccinia virus and poxvirology methods and protocols edited by Stuart N. Isaacs ; foreword by Bernard Moss Totowa, NJ Humana Press 2004 XIX, 396 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 269 Includes bibliographical references and index Poxviridae cabt Vaccinia virus cabt Poxvirus recombinants - Manuels de laboratoire Vaccine - Manuels de laboratoire DNA Replication Molecular Biology methods Poxviridae genetics Recombinant poxviruses Laboratory manuals Vaccinia virus genetics Vaccinia virus immunology Vaccinia Laboratory manuals Virus Replication Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Vaccinia-Virus (DE-588)4187306-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Vaccinia-Virus (DE-588)4187306-3 s Molekulargenetik (DE-588)4039987-4 s Labortechnik (DE-588)4123602-6 s b DE-604 Isaacs, Stuart Neal Sonstige oth Methods in molecular biology 269 (DE-604)BV035362695 269 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012815067&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Vaccinia virus and poxvirology methods and protocols Methods in molecular biology Poxviridae cabt Vaccinia virus cabt Poxvirus recombinants - Manuels de laboratoire Vaccine - Manuels de laboratoire DNA Replication Molecular Biology methods Poxviridae genetics Recombinant poxviruses Laboratory manuals Vaccinia virus genetics Vaccinia virus immunology Vaccinia Laboratory manuals Virus Replication Molekulargenetik (DE-588)4039987-4 gnd Labortechnik (DE-588)4123602-6 gnd Vaccinia-Virus (DE-588)4187306-3 gnd |
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| title | Vaccinia virus and poxvirology methods and protocols |
| title_auth | Vaccinia virus and poxvirology methods and protocols |
| title_exact_search | Vaccinia virus and poxvirology methods and protocols |
| title_full | Vaccinia virus and poxvirology methods and protocols edited by Stuart N. Isaacs ; foreword by Bernard Moss |
| title_fullStr | Vaccinia virus and poxvirology methods and protocols edited by Stuart N. Isaacs ; foreword by Bernard Moss |
| title_full_unstemmed | Vaccinia virus and poxvirology methods and protocols edited by Stuart N. Isaacs ; foreword by Bernard Moss |
| title_short | Vaccinia virus and poxvirology |
| title_sort | vaccinia virus and poxvirology methods and protocols |
| title_sub | methods and protocols |
| topic | Poxviridae cabt Vaccinia virus cabt Poxvirus recombinants - Manuels de laboratoire Vaccine - Manuels de laboratoire DNA Replication Molecular Biology methods Poxviridae genetics Recombinant poxviruses Laboratory manuals Vaccinia virus genetics Vaccinia virus immunology Vaccinia Laboratory manuals Virus Replication Molekulargenetik (DE-588)4039987-4 gnd Labortechnik (DE-588)4123602-6 gnd Vaccinia-Virus (DE-588)4187306-3 gnd |
| topic_facet | Poxviridae Vaccinia virus Poxvirus recombinants - Manuels de laboratoire Vaccine - Manuels de laboratoire DNA Replication Molecular Biology methods Poxviridae genetics Recombinant poxviruses Laboratory manuals Vaccinia virus genetics Vaccinia virus immunology Vaccinia Laboratory manuals Virus Replication Molekulargenetik Labortechnik Vaccinia-Virus Aufsatzsammlung |
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