Molecular genetics of bacteria:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Hoboken, NJ [u.a.]
Wiley
2004
|
| Edition: | 4. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XII, 346 S. Ill., graph. Darst. |
| ISBN: | 0470850841 047085085X |
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| 245 | 1 | 0 | |a Molecular genetics of bacteria |c Jeremy W. Dale ; Simon F. Park |
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Record in the Search Index
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| adam_text | Contents
1 Nucleic Acid Structure and Function 1
1.1 Structure of nucleic acids 1
1.1.1 DNA 1
1.1.2 RNA 3
1.1.3 Hydrophobic interactions 3
1.1.4 Different forms of the double helix 5
1.1.5 Supercoiling 6
1.1.6 Denaturation and hybridization 10
1.1.7 Orientation of nucleic acid strands 11
1.2 Replication of DNA 12
1.2.1 Unwinding and rewinding 13
1.2.2 Fidelity of replication: proof reading 13
1.3 Chromosome replication and cell division 14
1.4 DNA repair 19
1.4.1 Mismatch repair 19
1.4.2 Excision repair 19
1.4.3 Recombination (post replication) repair 19
1.4.4 SOS repair 20
1.5 Gene expression 21
1.5.1 Transcription 22
1.5.2 Translation 26
1.5.3 Post translational events 32
1.6 Gene organization 34
2 Mutation and Variation 37
2.1 Variation and evolution 37
2.1.1 Fluctuation test 38
2.1.2 Directed mutation in bacteria? 40
2.2 Types of mutations 41
2.2.1 Point mutations 41
2.2.2 Conditional mutants 42
2.2.3 Variation due to larger scale DNA alterations 44
2.2.4 Extrachromosomal agents and horizontal gene transfer 44
2.3 Pheno types 45
Molecular Genetics of Bacteria, 4th Edition by Jeremy Dale and Simon F. Park
© 2004 John Wiley Sons, Ltd ISBN 0 470 85084 1 (cased) ISBN 0 470 85085 X (pbk)
y| CONTENTS
47
2.4 Restoration of phenotype
2.4.1 Reversion and suppression 47
49
2.4.2 Complementation
2.5 Recombination
2.6 Mechanisms of mutation
2.6.1 Spontaneous mutation
2.6.2 Chemical mutagens
2.6.3 Ultraviolet irradiation
2.7 Isolation and identification of mutants
2.7.1 Mutation and selection
2.7.2 Replica plating ^
2.7.3 Penicillin enrichment
2.7.4 Isolation of other mutants
2.7.5 Molecular methods 62
3 Regulation of Gene Expression
3.1 Gene copy number
3.2 Transcriptional control
3.2.1 Promoters 70
3.2.2 Terminators, attenuators and anti terminators
79
3.2.3 Induction and repression: regulatory proteins
3.2.4 Attenuation: trp operon
3.2.5 Two component regulatory systems
3.2.6 Global regulatory systems
3.2.7 Feast or famine and the RpoS regulon *
3.2.8 Quorum sensing ^
3.3 Translational control
3.3.1 Ribosome binding
3.3.2 Codon usage 101
3.3.3 Stringent response 1
3.3.4 Regulatory RNA 102
3.3.5 Phase variation 102
4 Genetics of Bacteriophages 103
4.1 Single stranded DNA bacteriophages ^
4.1.1 J X174 106
4.1.2 M13 1°9
4.2 RNA containing phages: MS2 109
4.3 Double stranded DNA phages 110
4.3.1 Bacteriophage T4 H°
4.3.2 Bacteriophage lambda H3
4.3.3 Lytic and lysogenic regulation of bacteriophage lambda 118
4.4 Restriction and modification 125
4.5 Complementation and recombination 128
4.6 Why are bacteriophages important? 130
4.6.1 Phage typing 131
CONTENTS Vii
4.6.2 Phage therapy 131
4.6.3 Phage display 132
4.6.4 Bacterial virulence and phage conversion 133
5 Plasmids 137
5.1 Some bacterial characteristics are determined by plasmids 137
5.1.1 Antibiotic resistance 137
5.1.2 Colicins and bacteriocins 138
5.1.3 Virulence determinants 138
5.1.4 Plasmids in plant associated bacteria 139
5.1.5 Metabolic activities 139
5.2 Molecular properties of plasmids 141
5.2.1 Plasmid replication and control 143
5.3 Plasmid stability 154
5.3.1 Plasmid integrity 155
5.3.2 Partitioning 157
5.3.3 Differential growth rate 160
5.4 Methods for studying plasmids 161
5.4.1 Associating a plasmid with a phenotype 161
5.4.2 Classification of plasmids 163
6 Gene Transfer 165
6.1 Transformation 166
6.2 Conjugation 167
6.2.1 Mechanism of conjugation 168
6.2.2 The F plasmid 173
6.2.3 Conjugation in other bacteria 174
6.3 Transduction 178
6.3.1 Specialized transduction 180
6.4 Recombination 181
6.4.1 General (homologous) recombination 181
6.4.2 Site specific and non homologous (illegitimate) recombination 186
6.5 Mosaic genes and chromosome plasticity 187
7 Genomic Plasticity: Movable Genes and Phase Variation 189
7.1 Insertion sequences 189
7.1.1 Structure of insertion sequences 189
7.1.2 Occurrence of insertion sequences 190
7.2 Transposons 192
7.2.1 Structure of transposons 194
7.2.2 Integrons 196
7.3 Mechanisms of transposition 197
7.3.1 Replicative transposition 197
7.3.2 Non replicative (conservative) transposition 200
7.3.3 Regulation of transposition 201
7.3.4 Activation of genes by transposable elements 203
yjij CONTENTS
7.3.5 Mu: a transposable bacteriophage 204
7.3.6 Conjugative transposons and other transposable elements 205
7.4 Phase variation 205
7.4.1 Variation mediated by simple DNA inversion. 207
7.4.2 Variation mediated by nested DNA inversion 208
7.4.3 Antigenic variation in the gonococcus 208
7.4.4 Phase variation by slipped strand mispairing 211
7.4.5 Phase variation mediated by differential DNA methylation 214
8 Genetic Modification: Exploiting the Potential of Bacteria 215
8.1 Strain development 21^
8.1.1 Generation of variation 215
8.1.2 Selection of desired variants 216
8.2 Overproduction of primary metabolites 216
8.2.1 Simple pathways 217
8.2.2 Branched pathways 218
8.3 Overproduction of secondary metabolites 220
8.4 Gene cloning 221
8.4.1 Cutting and joining DNA 222
8.4.2 Plasmid vectors 223
8.4.3 Transformation 225
8.4.4 Bacteriophage lambda vectors 225
8.4.5 Cloning larger fragments 227
8.4.6 Bacteriophage M13 vectors 229
8.5 Gene libraries 229
8.5.1 Construction of genomic libraries 229
8.5.2 Screening a gene library 231
8.5.3 Construction of a cDNA library 233
8.6 Products from cloned genes 234
8.6.1 Expression vectors 234
8.6.2 Making new genes 236
8.6.3 Other bacterial hosts 239
8.6.4 Novel vaccines 241
8.7 Other uses of gene technology 242
9 Genetic Methods for Investigating Bacteria 245
9.1 Metabolic pathways 245
9.1.1 Complementation 246
9.1.2 Cross feeding 246
9.2 Microbial physiology 247
9.2.1 Reporter genes 249
9.2.2 Lysogeny 250
9.2.3 Cell division 251
9.2.4 Motility and chemotaxis 252
9.2.5 Cell differentiation 253
CONTENTS lX
9.3 Bacterial virulence 257
9.3.1 Wide range mechanisms of bacterial pathogenesis 257
9.3.2 Detection of virulence genes 259
9.4 Specific mutagenesis 262
9.4.1 Gene replacement 262
9.4.2 Antisense RNA 264
9.5 Taxonomy, evolution and epidemiology 264
9.5.1 Molecular taxonomy 264
9.5.2 Diagnostic use of PCR 267
9.5.3 Molecular epidemiology 267
10 Gene Mapping to Genomics 273
10.1 Gene mapping 273
10.1.1 Conjugational analysis 273
10.1.2 Co transformation and co transduction 276
10.1.3 Molecular techniques for gene mapping 277
10.2 Gene sequencing 279
10.2.1 DNA sequence determination 281
10.2.2 Genome sequencing 282
10.2.3 Comparative genomics 285
10.2.4 Bioinformatics 288
10.3 Physical and genetic maps 289
10.3.1 Deletions and insertions 290
10.3.2 Transposon mutagenesis 290
10.3.3 Gene replacement 292
10.3.4 Site directed mutagenesis 292
10.4 Analysis of gene expression 292
10.4.1 Transcriptional analysis 293
10.4.2 Translational analysis 296
10.4.3 Systematic analysis of gene function 300
10.5 Conclusion 300
Appendix A Further Reading 301
Appendix B Abbreviations 305
Appendix C Glossary 309
Appendix D Enzymes 323
Appendix E Genes 327
Appendix F Standard Genetic Code 331
Appendix G Bacterial Species 333
Index 337
|
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| discipline | Biologie |
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| spelling | Dale, Jeremy W. Verfasser aut Molecular genetics of bacteria Jeremy W. Dale ; Simon F. Park 4. ed. Hoboken, NJ [u.a.] Wiley 2004 XII, 346 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Bakterien (DE-588)4004296-0 s Molekulargenetik (DE-588)4039987-4 s DE-604 Gentechnologie (DE-588)4071722-7 s Park, Simon F. Verfasser aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010724511&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dale, Jeremy W. Park, Simon F. Molecular genetics of bacteria Molekulargenetik (DE-588)4039987-4 gnd Bakterien (DE-588)4004296-0 gnd Gentechnologie (DE-588)4071722-7 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4004296-0 (DE-588)4071722-7 |
| title | Molecular genetics of bacteria |
| title_auth | Molecular genetics of bacteria |
| title_exact_search | Molecular genetics of bacteria |
| title_full | Molecular genetics of bacteria Jeremy W. Dale ; Simon F. Park |
| title_fullStr | Molecular genetics of bacteria Jeremy W. Dale ; Simon F. Park |
| title_full_unstemmed | Molecular genetics of bacteria Jeremy W. Dale ; Simon F. Park |
| title_short | Molecular genetics of bacteria |
| title_sort | molecular genetics of bacteria |
| topic | Molekulargenetik (DE-588)4039987-4 gnd Bakterien (DE-588)4004296-0 gnd Gentechnologie (DE-588)4071722-7 gnd |
| topic_facet | Molekulargenetik Bakterien Gentechnologie |
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