mRNA processing and metabolism: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, N.J.
Humana Press
2004
|
| Series: | Methods in molecular biology
257 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XV, 270 S. graph. Darst. |
| ISBN: | 9781588292254 1588292258 |
Staff View
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface v
Contributors xiii
1 Using Chromatin Immunoprecipitation to Map Cotranscriptional
mRNA Processing in Saccharomyces cerevisiae
Michael Christopher Keogh and Stephen Buratowski /
2 Identifying PhosphoCTD Associating Proteins
Hemali P. Phatnani and Arno L. Greenleaf 17
3 Imaging Alternative Splicing in Living Cells
Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas,
and Mariano A. Garcia Blanco 29
4 Tobramycin Affinity Tag Purification of Spliceosomes
Klaus Hartmuth, Hans Peter Vornlocher,
and Reinhard Liihrmann 47
5 Using Single Strand Conformational Polymorphism Gel
Electrophoresis to Analyze Mutually Exclusive
Alternative Splicing
Alicia M. Celotto and Brenton R. Graveley 65
6 Methods for the Analysis of Adenosine to lnosine Editing in RNA
Zuo Zhang and Gordon G. Carmichael 75
7 Assaying Nuclear Messenger RNA Export in Human Cells
Bryan R. Cullen 85
8 RNA Unwinding Assay for DExD/H Box RNA Helicases
Stephanie S. Tseng Rogenski and Tien Hsien Chang 93
9 Approaches for Monitoring Nuclear Translation
Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook 103
10 Immunopurification and Analysis of Protein and RNA Components
of mRNP in Mammalian Cells
Fabrice Lejeune and Lynne E. Maquat 115
11 Gene Expression Analysis of Messenger RNP Complexes
Luiz O. F. Penalva, Scott A. Tenenbaum, and Jack D. Keene 125
12 Using the XN Peptide to Tether Proteins to RNAs
Julie Baron Benhamou, Niels H. Gehring, Andreas E. Kulozik,
and Matthias W. Hentze 135
xi
xii Contents
13 An Efficient System for Cap and Poly(A) Dependent Translation
In Vitro
Yuri V. Svitkin and Nahutn Sonenberg 155
14 A Poly(A) Tail Responsive In Vitro System for Cap or IRES Driven
Translation From HeLa Cells
Christian Thoma, Antje Ostareck Lederer,
and Matthias W. Hentze 171
15 Assessing Messenger RNA Decapping in Cellular Extracts
Naomi Bergman, Joseph Milone, Elizabeth J. Bates,
Mateusz Opyrchal, Vivian Bellofatto, and Jeffrey Wilusz 181
16 Analysis of RNA Exonucleolytic Activities in Cellular Extracts
Devi Mukherjee, David T. Fritz, Walter I. Kilpatrick, Min Gao,
and Jeffrey Wilusz 193
1 7 Application of Ligation Mediated Reverse Transcription
Polymerase Chain Reaction to the Identification of In Vivo
Endonuclease Generated Messenger RNA Decay Intermediates
Mark N. Hanson and Daniel R. Schoenberg 213
18 Biochemical Dissection of RNA Silencing in Plants
Guiliang Tang and Phillip D. Zamore 223
19 RNA Interference of mRNA Processing Factors in Drosophila
S2 Cells
Alicia M. Celotto and Brenton R. Graveley 245
20 RNA Interference by Short Hairpin RNAs Expressed
in Vertebrate Cells
Gregory J. Hannon and Douglas S. Conklin 255
Index 267
|
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| dewey-tens | 570 - Biology |
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| indexdate | 2024-07-09T19:21:53Z |
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| isbn | 9781588292254 1588292258 |
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| physical | XV, 270 S. graph. Darst. |
| publishDate | 2004 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | mRNA processing and metabolism methods and protocols edited by Daniel R. Schoenberg Totowa, N.J. Humana Press 2004 XV, 270 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 257 Includes bibliographical references and index Genetic Techniques Messenger RNA Laboratory manuals RNA, Messenger metabolism Messenger-RNS (DE-588)4169499-5 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Messenger-RNS (DE-588)4169499-5 s Biochemische Methode (DE-588)4319880-6 s DE-604 Schoenberg, Daniel R. Sonstige oth Methods in molecular biology 257 (DE-604)BV035362695 257 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010684154&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | mRNA processing and metabolism methods and protocols Methods in molecular biology Genetic Techniques Messenger RNA Laboratory manuals RNA, Messenger metabolism Messenger-RNS (DE-588)4169499-5 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| subject_GND | (DE-588)4169499-5 (DE-588)4319880-6 (DE-588)4143413-4 |
| title | mRNA processing and metabolism methods and protocols |
| title_auth | mRNA processing and metabolism methods and protocols |
| title_exact_search | mRNA processing and metabolism methods and protocols |
| title_full | mRNA processing and metabolism methods and protocols edited by Daniel R. Schoenberg |
| title_fullStr | mRNA processing and metabolism methods and protocols edited by Daniel R. Schoenberg |
| title_full_unstemmed | mRNA processing and metabolism methods and protocols edited by Daniel R. Schoenberg |
| title_short | mRNA processing and metabolism |
| title_sort | mrna processing and metabolism methods and protocols |
| title_sub | methods and protocols |
| topic | Genetic Techniques Messenger RNA Laboratory manuals RNA, Messenger metabolism Messenger-RNS (DE-588)4169499-5 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| topic_facet | Genetic Techniques Messenger RNA Laboratory manuals RNA, Messenger metabolism Messenger-RNS Biochemische Methode Aufsatzsammlung |
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