Cell biology of synapses at Drosophila larval neuromuscular junctions: role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Tübingen
Selbstverl.
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Tübingen, Univ., Diss., 2003. - Enth. Zsfassung in engl. und dt. Sprache |
| Beschreibung: | VIII, 98 S. Ill., graph. Darst 21 cm |
Internformat
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| 100 | 1 | |a Marella, Sunanda |d 1974- |e Verfasser |0 (DE-588)128387742 |4 aut | |
| 245 | 1 | 0 | |a Cell biology of synapses at Drosophila larval neuromuscular junctions |b role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping |c von Sunanda Marella |
| 264 | 1 | |a Tübingen |b Selbstverl. |c 2003 | |
| 300 | |a VIII, 98 S. |b Ill., graph. Darst |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zugl.: Tübingen, Univ., Diss., 2003. - Enth. Zsfassung in engl. und dt. Sprache | ||
| 650 | 0 | 7 | |a Larve |0 (DE-588)4166781-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Myosin |0 (DE-588)4170970-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Motorische Endplatte |0 (DE-588)4170600-6 |2 gnd |9 rswk-swf |
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| 689 | 0 | 2 | |a Motorische Endplatte |0 (DE-588)4170600-6 |D s |
| 689 | 0 | 3 | |a Myosin |0 (DE-588)4170970-6 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010609874 | |
Datensatz im Suchindex
| _version_ | 1808044662041083904 |
|---|---|
| adam_text |
CONTENTS
LIST
OF
FIGURES V
LIST
OF
TABLES
VI
ABSTRACT
VII
ZUSAMMENFASSUNG
VIII
1
:
INTRODUCTION
1
1.1
:
SYNAPTIC
PLASTICITY:
STRUCTURAL
AND
FUNCTIONAL
CHANGES
AT
SYNAPSES
IN
RESPONSE
TO
ACTIVITY
1
1.2:
THE
DROSOPMA
NMJ
YY
2
1.2.1
THE
LAYOUT
OF
THE
DROSOPHILA
NMJ
3
1.2.1.1
ORGANIZATION
OF
VARIOUS
COMPONENTS
OF
THE
NMJ
3
1.2.1.2
ULTRASTRUCTURE
OF
A
SYNAPTIC
BOUTON
4
1
.2.2
CELL
BIOLOGY
OF
NMJS
5
1.2.2.1
THE
PRESYNAPSE
5
1.2.2.2
THE
POSTSYNAPSE
8
1.2.2.3
TRANSYNAPTIC
INTERACTIONS
9
1.2.3
MECHANISMS
ADOPTED
IN
THE
MODIFICATION
OF
SYNAPSES
AT
THE
NMJ
10
1.2.3.1
DEVELOPMENTAL
MECHANISMS
10
1.2.3.2
ACTIVITY
DEPENDENT
MECHANISMS
12
1.2.3.2.1
GENETIC
MODELS
OF
SYNAPTIC
PLASTICITY
12
1.2.3.2.2
EXPERIENCE
DEPENDENT
SYNAPTIC
PLASTICITY
13
1.3
:
THE
DROSOPHILA
NMJ
AS
A
MODEL
SYSTEM
FOR
THE
STUDY
OF
SYNAPTIC
PLASTICITY
14
1.4:
OBJECTIVES
OF
THE
STUDY
14
2
:
A
SYNAPTIC
ROLE
FOR
THE
DROSOPHILA
MYOSIN
VILA
16
2.1
:
INTRODUCTION
16
2.2
:
RESULTS
19
2.2.1
MUTATIONS
IN
CRINKLED
SUPPRESS
TRANSLATION
INDUCED
OUTGROWTH
OF
NMJS
19
2.2.2
TRANSGENES
OF
CRINKLED
CAUSE
AND
RESCUE
SENSORY
BRISTLE
PHENOTYPES
19
2.2.3
CRINKLED
IS
LOCALIZED
IN
THE
SYNAPSE-ASSOCIATED
SUBSYNAPTIC
RETICULUM
OF
LARVAL
NMJS
21
2.2.4
CRINKLED
IS
NECESSARY
TO
MAINTAIN
THE
QUANTAL
SIZE
OF
LARVAL
NMJS
23
2.2.5
CRINKLED
MUTANTS
ENHANCE
PKA-MEDIATED
DGLUR-IIA
INACTIVATION
25
2.2.6
CRINKLED
MUTANTS
SUPPRESS
PRESYNAPTIC
COMPENSATION
FOR
REDUCED
QUANTAL
SIZE
26
2.2.7
CRINKLED
MUTANTS
SHOW
REDUCED
SUBSYNAPTIC
PROTEIN
SYNTHESIS
29
2.2.8
CRINKLED
INTERACTS
WITH
MEMBRANE
TRAFFICKING
MACHINERY
31
2.3:
DISCUSSION
34
2.3.1
CRINKLED
IS
EXPRESSED
IN
LARVAL
MUSCLES
AND
IS
LOCALIZED
AT
NMJS
34
2.3.2
MUTATIONS
IN
CRINKLED
CAUSES
PHYSIOLOGICAL
AND
MORPHOLOGICAL
PHENOTYPES
35
2.3.3
A
POSSIBLE
ROLE
PLAYED
BY
CRINKLED
AT
SYNAPSES
36
2.3.4
AN
ALTERNATIVE
HYPOTHESIS
39
2.3.5
FUTURE
DIRECTIONS
39
3
:
DYNAMIC
MODULATION
OF
SYNAPTIC
PROTEINS
41
3.1
:
INTRODUCTION
41
3.2:
RESULTS
43
3.2.1
ISOLATION
OF
GFP
TAGGED
PROTEINS
WHICH
LOCALIZE
AT
NMJS
43
3.2.2
IDENTIFYING
THE
MOLECULAR
NATURE
OF
THE
TAGGED
EXON
TRAP
INSERTIONS
45
3.2.3
CONFIRMING
THE
SPECIFICITY
OF
THE
MOLECULAR
NATURE
OF
THE
TAGGED
MOLECULE
48
3.2.4
DYNAMIC
CHANGES
IN
LOCALISATION
PATTERNS
IN
RESPONSE
TO
ACTIVITY
49
3.3
:
DISCUSSION
52
4
:
MATERIALS
AND
METHODS
55
4.1
:
REAGENTS
55
4.1.1
IMMUNOHISTOCHEMICAL
STAINING
OF
LARVAL
FILETS
55
II
4.1.2
MEDIA
FOR
BACTERIA
55
4.1.3
MEDIA
FOR
FLIES
55
4.1.4
MOLECULAR
BIOLOGY
55
4.1.4.1
DNA
ANALYSIS
55
4.1.4.2
RNA
ANALYSIS
56
4.1.5
PROTEIN
CHEMISTRY
56
4.1.5.1
BUFFERS
USED
FOR
PURIFICATION
OF
GST
TAGGED
PROTEINS
57
4.1.5.2
BUFFERS
USED
FOR
SOLUBILIZATION
OF
GFP
TAGGED
LARVAL
PROTEINS
57
4.1.5.3
BUFFERS
USED
FOR
WESTERN
BLOT
ANALYSIS
57
4.1.6
ANTIBODIES
58
4.1.7
OLIGONUCLEOTIDES
USED
IN
THIS
STUDY
60
4.1.8
VECTORS
USED
IN
THE
STUDY
62
4.1.9
FLY
STOCKS
64
4.2
:
GENERAL
PROTOCOLS
65
4.2.1
MOLECULAR
BIOLOGY
65
4.2.1.1
PURIFICATION
OF
PLASMID
DNA 66
4.2.1.2
PURIFICATION
AND
ANALYSIS
OF
DNA
FRAGMENTS
66
4.2.1.2.1
DNA
DIGESTION
WITH
RESTRICTION
ENDONUCLEASES
66
4.2.1.2.2
AGAROSE
GEL
ELECTROPHORESIS
66
4.2.1.2.3
PURIFICATION
OF
DNA
FRAGMENTS
FROM
AGAROSE
GELS
67
4.2.1.3
SUBCLONING
OF
DNA
FRAGMENTS
67
4.2.1.3.1
DEPHOSPHORYLATION
OF
DNA
FRAGMENTS
67
4.2.1.3.2
LIGATION
OF
DNA
FRAGMENTS
67
4.2.1.3.3
TRANSFORMATION
OF
COMPETENT
BACTERIA
WITH
PLASMIDS
67
4.2.1.3.4
BACTERIAL
GLYCEROL
CULTURES
(
STABS')
68
4.2.1.4
POLYMERASE
CHAIN
REACTION
(PCR)
68
4.2.1.5
CONSTRUCTS
69
4.2.1.6
INVERSE
PCR
71
4.2.1.6.1
GENOMIC
DNA
PREPARATION
FROM
ADULT
FLIES
71
4.2.1.6.2
RESTRICTION
DIGESTS
71
4.2.1.6.3
LIGATION
71
4.2.1.6.4
PCR
72
4.2.1.7
MAKING
RNA
PROBES
FOR
IN
SITU
HYBIRDIZATION
72
4.2.2
PROTEIN
CHEMISTRY
73
4.2.2.1
SDS-PAGE
ELECTROPHORESIS
73
4.2.2.2
PURIFICATION
OF
GST
FUSION
PROTEINS
73
4.2.2.3
GST-PULLDOWNS
74
4.3
:
FLY
WORK
75
4.3.1
FLY
STOCK
MAINTENANCE
75
4.3.2
GENETIC
CROSSES
FOR
OPTIMAL
LARVAL
CULTURES
75
4.3.3
TARGETED
EXPRESSION
OF
GENES
IN
LARVAE
75
4.3.4
GENETIC
RECOMBINATION
76
4.3.5
GENERATION
OF
TRANSGENIC
LINES
76
4.3.5.1
INJECTIONS
76
4.3.5.2
TRANGENIC
LINES
MAPPED
TO
SINGLE
CHROMOSOMES
76
4.3.6
RNA
IN
SITU
HYBRIDIZATION
4.3.6.1
FIXING
OF
EMBRYOS
FOR
IN
SITU
HYBRIDIZATION
4.3.6.2
FIXING
OF
LARVAE
FOR
IN
SITU
HYBRIDIZATION
4.3.6.3
RNA
IN
SITU
4.3.7
ANTIBODY
STAININGS
4.3.7.1
FIXING
OF
LARVAE
FOR
ANTIBODY
STAININGS
78
4.3.7.2
IMMUNOHISTOCHEMICAL
STUDIES
78
4.4:
ELECTROPHYSIOLOGY
78
BIBLIOGRAPHY
79
ACKNOWLEDGEMENTS
97
LEBENSLAUF
98
T
E
IV |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017641194 |
| illustrated | Illustrated |
| indexdate | 2024-08-22T00:15:55Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010609874 |
| oclc_num | 645368918 |
| open_access_boolean | |
| owner | DE-29T DE-12 |
| owner_facet | DE-29T DE-12 |
| physical | VIII, 98 S. Ill., graph. Darst 21 cm |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Selbstverl. |
| record_format | marc |
| spelling | Marella, Sunanda 1974- Verfasser (DE-588)128387742 aut Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping von Sunanda Marella Tübingen Selbstverl. 2003 VIII, 98 S. Ill., graph. Darst 21 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: Tübingen, Univ., Diss., 2003. - Enth. Zsfassung in engl. und dt. Sprache Larve (DE-588)4166781-5 gnd rswk-swf Myosin (DE-588)4170970-6 gnd rswk-swf Motorische Endplatte (DE-588)4170600-6 gnd rswk-swf Drosophila (DE-588)4150708-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Drosophila (DE-588)4150708-3 s Larve (DE-588)4166781-5 s Motorische Endplatte (DE-588)4170600-6 s Myosin (DE-588)4170970-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010609874&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Marella, Sunanda 1974- Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping Larve (DE-588)4166781-5 gnd Myosin (DE-588)4170970-6 gnd Motorische Endplatte (DE-588)4170600-6 gnd Drosophila (DE-588)4150708-3 gnd |
| subject_GND | (DE-588)4166781-5 (DE-588)4170970-6 (DE-588)4170600-6 (DE-588)4150708-3 (DE-588)4113937-9 |
| title | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping |
| title_auth | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping |
| title_exact_search | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping |
| title_full | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping von Sunanda Marella |
| title_fullStr | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping von Sunanda Marella |
| title_full_unstemmed | Cell biology of synapses at Drosophila larval neuromuscular junctions role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping von Sunanda Marella |
| title_short | Cell biology of synapses at Drosophila larval neuromuscular junctions |
| title_sort | cell biology of synapses at drosophila larval neuromuscular junctions role of the unconventional myosin viia and identification of synaptic molecules involved in dynamic changes by gfp exon trapping |
| title_sub | role of the unconventional Myosin VIIa and identification of synaptic molecules involved in dynamic changes by GFP Exon trapping |
| topic | Larve (DE-588)4166781-5 gnd Myosin (DE-588)4170970-6 gnd Motorische Endplatte (DE-588)4170600-6 gnd Drosophila (DE-588)4150708-3 gnd |
| topic_facet | Larve Myosin Motorische Endplatte Drosophila Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010609874&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT marellasunanda cellbiologyofsynapsesatdrosophilalarvalneuromuscularjunctionsroleoftheunconventionalmyosinviiaandidentificationofsynapticmoleculesinvolvedindynamicchangesbygfpexontrapping |