Transcriptional regulation of the Xenopus MyoD Gene:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2003
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | München, Univ., Diss., 2003 |
| Physical Description: | IV, 114 S., Ill., graph. Darst. |
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MARC
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| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 |a DE-12 | ||
| 100 | 1 | |a Xiao, Lei |d 1973- |e Verfasser |0 (DE-588)124903878 |4 aut | |
| 245 | 1 | 0 | |a Transcriptional regulation of the Xenopus MyoD Gene |c von Lei Xiao |
| 264 | 1 | |c 2003 | |
| 300 | |a IV, 114 S., Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a München, Univ., Diss., 2003 | ||
| 650 | 4 | |a Krallenfrosch - Muskelentwicklung - Genregulation | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010565230 | |
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| _version_ | 1807772306211078144 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
1
SUMMARY
1
2
INTRODUCTION
2
2.1
EARLY
DEVELOPMENT
AND
MESODERM
PATTERNING
OF
XENOPUS
2
2.1.1
FROG
EMBRYOLOGY
2
2.1.2
MESODERM
INDUCTION
4
2.2
MUSCLE
DEVELOPMENT
6
2.3
MRF
EXPRESSION
IN
VERTEBRATE
EMBRYOS
7
2.3.1
MRF
EXPRESSION
IN
MUSCLE
PROGENITORS
OF
THE
MOUSE
EMBRYO
7
2.3.2
MRF
EXPRESSION
IN
MUSCLE
PROGENITORS
OF
THE
FROG
EMBRYO
8
2.4
FUNCTIONAL
AND
GENETIC
RELATIONSHIPS
OF
THE
MRFS
9
2.4.1
MYOGENIC
FUNCTIONS
OF
MRFS
9
2.4.2
GENETIC
ANALYSIS
OF
MYF5
AND
MYOD
FUNCTIONS
IN
PROGENITOR
SPECIFICATION
10
2.4.3
MYOGENIN
AND
MRF4
ARE
REGULATORS
OF
MUSCLE
DIFFERENTIATION
10
2.5
MYOGENIC
COMPETENCE
11
2.6
MOLECULAR
REGULATORY
MECHANISMS
OF
MYF5
AND
MYOD
12
2.6.1
MODULAR
REGULATION
OF
MYF5
AND
MYOD
BY
TRANSCRIPTION
ENHANCER
CASSETTES
12
2.6.1.1
TRANSCRIPTION
ENHANCERS
FOR
MYOD
EXPRESSION
IN
MUSCLE
PROGENITORS
AND
DIFFERENTIATING
MUSCLE
IN
MAMMALS
12
2.6.1.2
LINEAGE-SPECIFIC
MYF5
TRANSCRIPTION
ENHANCERS
13
2.7
DEVELOPMENTAL
SIGNALING
THAT
CONTROLS
MRF
EXPRESSION
14
2.7.1
MYOGENIC
SIGNALS
IN
MICE
14
2.7.2
MYOGENIC
SIGNALS
IN
XENOPUS
16
2.8
PRELIMINARY
WORK
ON
THE
TRANSCRIPTIONAL
REGULATION
OF
THE
XMYODB
GENE
IN
XENOPUS
18
2.9
THE
AIM
OF
THIS
WORK
21
3.
MATERAL
AND
METHODS
22
3.1
REAGENTS
22
3.2
DEVICES
22
TABLE
OF
CONTENTS
3.3
NUCLEIC
ACIDS
23
3.3.1
SIZE
STANDARDS
23
3.3.2
OLIGONUCLEOTIDES
23
3.3.2.1
OLIGONUCLEOTIDES
FOR
RT-PCR
23
3.3.2.2
OLIGONUCLEOTIDES
FOR
CLONING
24
3.3.2.3
OLIGONUCLEOTIDES
FOR
MUTATION
24
3.3.3
PLASMIDS
26
3.3.3.1
PLASMIDS
FOR
IN
VITRO
TRANSCRIPTION
TO
SYNTHESIZE
MRNA
26
3.3.3.2
PLASMIDS
FOR
DIG-LABELED
RNA
IN
SITU
HYBRIDIZATION
PROBES
27
3.3.3.3
REPORTER
GENE
CONSTRUCTS
28
3.3.3.4
PLASMIDS
FOR
TRANSGENESIS
29
3.4
BACTERIA
MANIPULATION
29
3.5
EMBRYOLOGICAL
METHODS
29
3.5.1
SOLUTIONS
29
3.5.2
EXPERIMENTAL
ANIMALS
30
3.5.3
SUPEROVULATION
OF
THE
FEMALE
XENOPUS LAEVIS
30
3.5.4
PREPARATION
OF
THE
TESTIS
30
3.5.5
IN
VITRO
FERTILIZATION
OF
EGGS
AND
CULTURE
OF
THE
EMBRYOS
30
3.5.6
DEJELLY
WITH
CYSTEIN
SOLUTION
31
3.5.7
INJECTION
OF
EMBRYOS
31
3.5.8
PREPARATION
OF
EXPLANTS
31
3.6
HISTOLOGICAL
METHODS
31
3.6.1
SOLUTION
31
3.6.2
FIXATION
OF
EMBRYOS
32
3.6.3
IMMUNOCYTOCHEMISTRY
32
3.6.4
LACZ
STAINING
33
3.7
SDS-PAGE
AND
WESTERN
BLOTTING
33
3.8
MOLECULAR
BIOLOGICAL
METHODS
33
3.8.1
SOLUTIONS
33
3.8.2
ISOLATION
OF
NUCLEIC
ACIDS
35
3.8.2.1
MINI-PREPARATION
WITH
QIAGEN
KIT
35
3.8.2.2
ISOLATION
OF
RNA
35
3.8.3
ANALYSIS
AND
MANIPULATION
OF
NUCLEIC
ACIDS
35
3.8.3.1
GEL
ELECTROPHORESIS
OF
NUCLEIC
ACIDS
35
3.8.3.2
ISOLATION
OF
DNA
FRAGMENTS
FROM
AGAROSE
GEL
36
3.8.3.3
CLONING
METHODS
36
3.8.4
POLYMERASE
CHAIN
REACTION
(PCR)
36
3.8.4.1
PCR
AMPLIFICATION
OF
DNA
FRAGMENTS
FOR
CLONING
36
3.8.4.2
RT-PCR
36
3.8.5
IN
VITRO
TRANSCRIPTION
36
3.8.5.1
IN
VITRO
REVERSE
TRANSCRIPTION
37
II
TABLE
OF
CONTENTS
3.8.5.2
IN
VITRO
TRANSCRIPTION
FOR
THE
INJECTION
37
3.8.5.3
IN
VITRO
TRANSCRIPTION
OF
DIG
LABELED
RNA
PROBES
37
3.8.6
SITE-DIRECTED
MUTAGENESIS
38
3.8.7
RNA
IN
SITU
HYBRIDIZATION
39
3.9
GENERATION
OF
TRANSGENEN
EMBRYOS
BY
RESTRICTION-ENZYME-MEADIAD-INTEGRATION
(REMI)
41
3.9.1
INTRODUCTION
41
3.9.2
HIGH
SPEED
EXTRACT
PREPARATION
41
3.9.3
SPERM NUCLEI
PREPARATION
45
3.9.4
PREPARATION
OF
DNA,
NEEDLES
AND
EQUIPMENT
47
3.9.4.1
PREPARATION
OF
LINEARISED
DNA
47
3.9.4.2
PREPARATION
OF
INJECTION
NEEDLES
FOR
NUCLEAR
TRANSPLANTATION
47
3.9.4.3
AGAROSE-COATED
INJECTION
DISHES
48
3.9.4.4
TRANSPLANTATION
APPARATUS
48
3.9.4.5
TRANSGENESIS
BY
SPERM
NUCLEAR
TRANSPLANTATION
INTO
UNFERTILISED
EGGS
49
4
RESULTS
53
4.1
ANALYSIS
OF
XMYOD
CIS-REGULATORY
ELEMENTS
53
4.1.1
THE
EXPRESSION
OF
XMYOD
AT
NF10.5
IS
REGULATED
BY
MULTIPLE
ELEMENTS
53
4.1.2
THE
-840/-704
REGION
CONTAINS
BOTH
ACTIVATING
AND
REPRESSING
ELEMENTS
57
4.1.3
FINE-SCALE
MAPPING
OF
MIE
57
4.1.4
MIE
IS
ESSENTIAL
FOR
EFGF
TO
ACTIVE
XMYOD
AT
NF
10.5
60
4.1.5
XCAD-3
AS
POTENTIAL
REGULATOR
OF
XMYOD
TRANSCRIPTION
62
4.1.6
A
SERUM
RESPONSE
ELEMENT
(SRE)
IS
ESSENTIAL
TO
MAINTAIN
XMYOD
64
TRANSCRIPTION
4.1.7
EFFECTS
OF
SRF-INTERFERENCE
ANALYSIS ON
XMYOD
EXPRESSION
67
4.1.8
ANALYSIS
OF
TCF
BINDING
SITE
AND
FAST
BINDING
SITE
71
4.1.9
NON-CODING
RNA
TRANSCRIPTED
IN
5
'
REGION
OF
XMYOD
GENOMIC
SEQUENCE
71
4.1.10
THE
TRANSCRIPTS
ARE
PRODUCED
BY
A
REPETITIVE
SEQUENCE
73
4.2
ANALYSIS
OF
POTENTIAL
REGULATORS
OF
XMYOD
EXPRESSION
76
4.2.1
XSEB-4
76
4.2.1.1
THE
SUBCELLULAR
LOCALIZATION
OF
XSEB-4
77
4.2.1.2
XSEB-4
INDUCES
XMYOD
EXPRESSION
IN
ANIMAL
CAP
ASSAY
79
4.2.2
YY-1
80
4.2.3
LEF-1
81
5
DISCUSSION
86
5.1
METHODOLOGICAL
CONSIDERATION
86
5.2
CIS-REGULATORY
ELEMENTS
THAT
REGULATE
THE
EXPRESSION
OF
XMYOD
88
III
TABLE
OF
CONTENTS
5.2.1
EXPRESSION
OF
XMYOD
IS
REGULATED
THROUGH
ACTIVATION
AND
REPRESSION
88
5.2.2
LS-5
MOTIF
89
5.2.3
LS-9
MOTIF
90
5.2.3.1
THE
LS-9
MOTIF
FUNCTIONS
AS
A
SILENCER
90
5.2.3.2
TEMPORAL
REGULATION
OF
THE
XMYOD
GENE
91
5.2.3.3
XVENTS
MAY
REGULATE
XMYOD
TRANSCRIPTION
VIA
LS-9
92
5.2.4
MAINTENANCE
ENHANCER
92
5.3
THE
EXPRESSION
PATTERN
OF
M-SIRT
CORRELATES
WITH
THE
INACTIVE
STATE
OF
XMYOD
GENE
94
5.4
POTENTIAL
PROTEIN
FACTORS
THAT
REGULATE
THE
EXPRESSION
OF
XMYOD
94
5.4.1
PUTATIVE
RNA
BINDING
PROTEIN
XSEB4
IS
ABLE
TO
INDUCE
XMYOD
EXPRESSION
IN
AN
ANIMAL
CAP
ASSAY
95
5.4.2
XYY1
95
5.4.3
LEF
1
IS
NECESSARY
AND
SUFFICIENT
FOR
THE
EXPRESSION
OF
XMYOD
96
5.5
CONSERVATION
OF
MYOD
REGULATION
96
5.6
A
MODEL
OF
THE
EPIGENETIC
REGULATION
OF
XMYOD
97
5.7
CONCLUSION
AND
OUTLOOK
100
6
LITERATURES
102
ABBREVIATIONS
CV
IV |
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| author_GND | (DE-588)124903878 |
| author_facet | Xiao, Lei 1973- |
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| author_sort | Xiao, Lei 1973- |
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| bvnumber | BV017551060 |
| ctrlnum | (OCoLC)249128284 (DE-599)BVBBV017551060 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017551060 |
| illustrated | Not Illustrated |
| indexdate | 2024-08-19T00:06:57Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010565230 |
| oclc_num | 249128284 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-12 |
| owner_facet | DE-19 DE-BY-UBM DE-12 |
| physical | IV, 114 S., Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| record_format | marc |
| spelling | Xiao, Lei 1973- Verfasser (DE-588)124903878 aut Transcriptional regulation of the Xenopus MyoD Gene von Lei Xiao 2003 IV, 114 S., Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2003 Krallenfrosch - Muskelentwicklung - Genregulation Muskelentwicklung (DE-588)4170853-2 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Krallenfrosch (DE-588)4165479-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Krallenfrosch (DE-588)4165479-1 s Muskelentwicklung (DE-588)4170853-2 s Genregulation (DE-588)4122166-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010565230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Xiao, Lei 1973- Transcriptional regulation of the Xenopus MyoD Gene Krallenfrosch - Muskelentwicklung - Genregulation Muskelentwicklung (DE-588)4170853-2 gnd Genregulation (DE-588)4122166-7 gnd Krallenfrosch (DE-588)4165479-1 gnd |
| subject_GND | (DE-588)4170853-2 (DE-588)4122166-7 (DE-588)4165479-1 (DE-588)4113937-9 |
| title | Transcriptional regulation of the Xenopus MyoD Gene |
| title_auth | Transcriptional regulation of the Xenopus MyoD Gene |
| title_exact_search | Transcriptional regulation of the Xenopus MyoD Gene |
| title_full | Transcriptional regulation of the Xenopus MyoD Gene von Lei Xiao |
| title_fullStr | Transcriptional regulation of the Xenopus MyoD Gene von Lei Xiao |
| title_full_unstemmed | Transcriptional regulation of the Xenopus MyoD Gene von Lei Xiao |
| title_short | Transcriptional regulation of the Xenopus MyoD Gene |
| title_sort | transcriptional regulation of the xenopus myod gene |
| topic | Krallenfrosch - Muskelentwicklung - Genregulation Muskelentwicklung (DE-588)4170853-2 gnd Genregulation (DE-588)4122166-7 gnd Krallenfrosch (DE-588)4165479-1 gnd |
| topic_facet | Krallenfrosch - Muskelentwicklung - Genregulation Muskelentwicklung Genregulation Krallenfrosch Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010565230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT xiaolei transcriptionalregulationofthexenopusmyodgene |