DNA sequence comparisons between humans and chimpanzees:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2003
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Leipzig, Univ., Diss., 2003 |
| Physical Description: | 109 S. Ill., graph. Darst. |
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MARC
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Record in the Search Index
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| adam_text |
CONTENTS
1 INTRODUCTION 9
2 MATERIALS AND METHODS 17
2.1 MATERIALS . 17
2.1.1 CHEMICALS. 17
2.1.2 ENZYMES. 18
2.1.3 BUFFERS. 18
2.1.4 KITS. 19
2.1.5 NUCLEIC ACIDS AND DNTPS. 19
2.1.5.1 GENOMIC DNAS. 19
2.1.5.2 VECTORS . 19
2.1.5.3 DNTPS . 19
2.15.4 SEQUENCING AND PCR PRIMER. 20
2
.
1.5.5 GENOMIC LIBRARIES. 21
2
.
1.0 TECHNICAL EQUIPMENT. 22
2.2 METHODS. 22
2.2.1 BAC LIBRARY SCREEN. 22
2.2.2 DNA AMPLIFICATION. 22
2.2.3 DNA CLONING AND PREPARATION OF SHOTGUN DNA LIBRARIES 23
2.2.4 PREPARATION OF PLASMID DNA. 23
2.2.5 DNA SEQUENCING . 23
2.2.6 CONTIG BUILDING AND QUALITY ASSESSMENT. 24
2.2.7 IDENTIFICATION OF REPETITIVE ELEMENTS. 24
2.2.8 PAIRWISE DNA SEQUENCE ALIGNMENT. 24
2.2.9 MULTIPLE DNA SEQUENCE ALIGNMENT. 25
2.2.10 DNA SEQUENCE ANALYSIS. 25
2.2.10.1 SUBSTITUTIONAL DNA SEQUENCE DIFFERENCES . 25
2.2.10.2 INSERTIONS AND DELETIONS. 27
2.2.11 DETERMINATION OF THE MALE TO FEMALE MUTATION RATE RATIO 28
2.2.12 DATA STORAGE. 29
2.2.13 ANNOTATION TRACK FOR THE HUMAN GENOME.29
2.3 ELECTRONIC DATABASE INFORMATION. 29
5
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/968709435
6
CONTESTS
3 RESULTS 31
3.1 GENOME-WIDE DXA SEQUENCE COMPARISON BETWEEN HUMANS AND
CHIMPANZEES. 31
3.1.1 IDENTIFICATION OF HOMOLOGOUS DXA SEQUENCES IN HUMANS 31
3.1.1.1 CHOICE OF THE HUMAN GENOME ASSEMBLY . 31
3.1.1.2 ALIGNMENT TO THE HUMAN GENOME.32
3.1.1.3 DATA VISUALIZATION. 33
3.1.2 DXA SEQUENCE DIFFERENCES. 33
3.1.2.1 GENOME-WIDE DIFFERENCES.33
3.1.2.2 ANALYSIS BY CHROMOSOME.35
3.1.2.3 DISTRIBUTION OF DXA SEQUENCE DIFFERENCES AMONG
THE AUTOSOMES. 36
3.2 REGION-SPECIFIC VARIATION IN THE SUBSTITUTION RATE AND SUBSTI
TUTION PATTERN. 38
3.2.1 ISOLATION OF THE ZFY AND ZFX GENOMIC REGIONS.39
3.2.2 DETERMINATION OF THE CONSENSUS SEQUENCES .39
3.2.3 DX
TA SEQUENCE ALIGNMENT.40
3.2.4 DNA SEQUENCE DIFFERENCES .41
3.2.4.1 NUCLEOTIDE SUBSTITUTIONS.41
3.2.4.2 INSERTIONS AND DELETIONS.44
4 DISCUSSION 49
4.1 GENOME-WIDE DNA SEQUENCE COMPARISON.49
4.1.1 AVERAGE AMOUNT OF DNA SEQUENCE DIFFERENCES.49
4.1.2 PATTERN OF DNA SEQUENCE DIFFERENCES.50
4.1.3 SUBSTITUTION PATTERN WITHIN AND BETWEEN SPECIES . 51
4.1.4 PATTERNS OF SEQUENCE DIFFERENCES AMONG CHROMOSOMES . 52
4.1.4.1 HIGHER MUTATION RATE IN THE MALE GERM LINE . 52
4.1.4.2 DIFFERENCES IN THE EVOLUTIONARY RATE AMONG
AUTOSOMES.60
4.2 REGIONAL DIFFERENCES IN THE SUBSTITUTION PATTERN .61
4.2.1 TERMINOLOGY.61
4.2.2 CORRELATION BETWEEN MUTATION RATE AND SUBSTITUTION RATE 62
4.2.3 REGIONAL DIFFERENCES IN THE SUBSTITUTION RATE.62
4.2.4 CORRELATION OF BASE COMPOSITION AND NUCLEOTIDE SUBSTI
TUTION PROCESS. 63
4.2.5 MODELS EXPLAINING REGIONAL DIFFERENCES IN THE SUBSTITU
TION PATTERN AND BASE COMPOSITION. 63
4.2.6 THE IDEAL DATA SET.65
4.2.7 RELATIVE SUBSTITUTION RATES IN THE ZFX AND ZFY REGIONS 66
4.2.8 REGIONAL SUBSTITUTION PATTERN AND GC CONTENT.67
4.2.9 SUBSTITUTIONS IN CPG CONTEXT.67
4.2.10 FACTORS THAT INFLUENCE THE REGIONAL SUBSTITUTION PATTERN
IN THE ZFX AND ZFY REGION.69
4.2.11 CHOICE OF THE MODEL .69
CONTENTS
7
4.2.11.1 EXCLUSION OF BIASED GENE CONVERSION.69
4.2.11.2 EXCLUSION OF NATURAL SELECTION. 71
4.2.11.3 MUTATION BIAS AS THE MODEL OF CHOICE.71
4.2.12 EQUILIBRIUM BASE FREQUENCY AND MUTATION BIAS.72
4.2.13 SUBSTITUTION MODEL. 72
4.2.14 SUBSTITUTION PATTERN PREDICTED AND SEEN . 74
4.2.15 SUBSTITUTION PATTERN AND GENOMIC LANDSCAPE. 76
4.2.16 EQUILIBRIUM BASE FREQUENCY AS A DETERMINANT OF THE
LOCAL SUBSTITUTION RATE IN THE HUMAN GENOME.77
4.2.17 CONCLUSION. 78
4.3 INSERTIONS AND DELETIONS.80
4.3.1 NUMBER OF INSERTIONS AND DELETIONS. 81
4.3.2 CHROMOSOME SPECIFIC DIFFERENCES IN THE INSERTION/DELOTION
RATE . 81
4.3.3 CONCLUSION. 85
5 SUMMARY 87
BIBLIOGRAPHY 91
A DATABASE SCHEME 101
B ABBREVIATIONS 103
C ACKNOWLEDGMENTS 105
D CURRICULUM VITAE 107
E STATEMENT OF INDEPENDENCE 109 |
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| indexdate | 2024-11-22T17:38:15Z |
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| language | English |
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| physical | 109 S. Ill., graph. Darst. |
| publishDate | 2003 |
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| spelling | Ebersberger, Ingo 1969- Verfasser (DE-588)124960111 aut DNA sequence comparisons between humans and chimpanzees Ingo Ebersberger 2003 109 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Leipzig, Univ., Diss., 2003 DNS-Sequenz (DE-588)4150352-1 gnd rswk-swf Schimpanse (DE-588)4179632-9 gnd rswk-swf Mensch (DE-588)4038639-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Schimpanse (DE-588)4179632-9 s Mensch (DE-588)4038639-9 s DNS-Sequenz (DE-588)4150352-1 s b DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010539695&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ebersberger, Ingo 1969- DNA sequence comparisons between humans and chimpanzees DNS-Sequenz (DE-588)4150352-1 gnd Schimpanse (DE-588)4179632-9 gnd Mensch (DE-588)4038639-9 gnd |
| subject_GND | (DE-588)4150352-1 (DE-588)4179632-9 (DE-588)4038639-9 (DE-588)4113937-9 |
| title | DNA sequence comparisons between humans and chimpanzees |
| title_auth | DNA sequence comparisons between humans and chimpanzees |
| title_exact_search | DNA sequence comparisons between humans and chimpanzees |
| title_full | DNA sequence comparisons between humans and chimpanzees Ingo Ebersberger |
| title_fullStr | DNA sequence comparisons between humans and chimpanzees Ingo Ebersberger |
| title_full_unstemmed | DNA sequence comparisons between humans and chimpanzees Ingo Ebersberger |
| title_short | DNA sequence comparisons between humans and chimpanzees |
| title_sort | dna sequence comparisons between humans and chimpanzees |
| topic | DNS-Sequenz (DE-588)4150352-1 gnd Schimpanse (DE-588)4179632-9 gnd Mensch (DE-588)4038639-9 gnd |
| topic_facet | DNS-Sequenz Schimpanse Mensch Hochschulschrift |
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