Proteomics and nanocrystallography:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York [u.a.]
Kluwer Academic / Plenum Publ.
2003
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XI, 190 S. Ill., graph. Darst. |
| ISBN: | 0306479028 |
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| 650 | 4 | |a Crystallography | |
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Record in the Search Index
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| adam_text | PROTEOMICS AND NANOCRYSTALLOGRAPHY EUGENIA PECHKOVA AND CLAUDIO NICOLINI
FONDAZIONE ELBA ROME, ITALY, AND UNIVERSITY OF GENOVA GENOVA, ITALY
KLUWER ACADEMIC / PLENUM PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON,
MOSCOW CONTENTS THE NEW FRONTIER AT THE CROSSING OF LIFE AND PHYSICAL
SCIENCES 1 STATE OF THE ART IN PROTEOMICS, CRYSTALLOGRAPHY AND
NANOBIOTECHNOLOGY 9 2.1 METHODS OF PROTEIN PRODUCTION 10 2.1.1
RECOMBINANT DNA TECHNOLOGY: EXPRESSION, PURIFICATION AND MUTAGENESIS 11
2.2 METHODS FOR PROTEIN IDENTIFICATION 15 2.2.1 TWO-DIMENSIONAL
ELECTROPHORESIS 16 2.2.2 MASS SPECTROMETRY AND ITS PRINCIPLES 17 2.3
METHODS OF PROTEIN CRYSTALLIZATION 20 2.3.1. IMPORTANCE OF PROTEIN
SAMPLE PREPARATION 21 2.3.2 PROTEIN SOLUBILITY AND SUPERSATURATION.
NUCLEATION AND CRYSTAL GROWTH 22 2.3.3 CLASSICAL METHODS 25 2.3.4 LESS
COMMON TECHNIQUES 27 2.3.5 ADVANCED METHODS 30 2.4 METHODS OF CRYSTAL
STRUCTURE DETERMINATION 35 2.4.1 PROTEIN X-RAY CRYSTALLOGRAPHY 35 2.4.2
CRYSTAL STRUCTURE DETERMINATION 38 2.4.3 DATA COLLECTION AND DATA
PROCESSING 39 2.4.4 TRADITIONAL X-RAY DIFFRACTOMETER 41 2.4.5 ELECTRON
MICROSCOPY 42 2.5 OTHER METHODS OF PROTEIN STRUCTURE INVESTIGATION 42 IX
CONTENTS 2.5.1 CIRCULAR DICHROISM MEASUREMENTS 42 2.5.2 HIGH RESOLUTION
NUCLEAR MAGNETIC RESONANCE 44 2.6 METHODS OF PROTEIN IMMOBILIZATION IN
SOLID THIN FILMS 50 2.6.1 PRESSURE-AREA ISOTHERMS 53 2.6.2 SURFACE
POTENTIAL 56 2.6.3 METHODS OF THIN FILM DEPOSITION 57 2.6.4 METHODS OF
THIN FILM CHARACTERIZATION 61 NANOCRYSTALLOGRAPHY 63 3.1 NANOBIOFILMS
TEMPLATE 63 3.1.1 SUBSTRATE PREPARATION 65 3.1.2 PROTEIN THIN FILM
FORMATION 65 3.1.3 PROTEIN THIN FILM CHARACTERIZATION 66 3.2 ATOMIC
FORCE MICROSCOPY 67 3.2.1 DESIGN OF THE CHAMBER 69 3.2.2 THE HARDWARE
AND SOFTWARE APPARATUS 71 3.2.3 PERFORMANCES OF THE SYSTEM 73 3.3
SYNCHROTRON RADIATION 78 3.3.1 PRINCIPLES OF SYNCHROTRON FUNCTIONING 80
3.3.2 MICROFOCUS BEAMLINE AND ULTRAMICROCRYSTAL DIFFRACTION 87 3.3.3
PROTEIN CRYSTALS SYNCHROTRON RADIATION ANALYSIS 95 3.3.4 STRUCTURE
DETERMINATION AND REFINEMENT 97 3.4 FROM ART TO SCIENCE WITH PROTEIN
NANOCRYSTALLOGRAPHY 97 FROM ART TO SCIENCE IN PROTEIN CRYSTALLOGRAPHY 99
4.1 NEW METHOD VALIDATION WITH LYSOZYME AS A MODEL PROTEIN 99 4.1.1
EFFECT OF THE THIN FILM TEMPLATE: KINETICS OF THE LYSOZYME CRYSTAL
GROWTH 101 4.1.2 THIN FILM SURFACE PRESSURE INFLUENCE ON THE
TEMPLATE-INDUCED CRYSTALLIZATION 105 4.1.3 X-RAY DIFFRACTION OF LYSOZYME
CRYSTALS 107 4.1.4 SYNCHROTRON RADIATION ANALYSIS OF LYSOZYME CRYSTALS
109 4.2 YET UNRESOLVED PROTEINS: BOVINE CYTOCHROME P450SCC ILL 4.2.1
CYTOCHROME P450SCC (SIDE-CHAIN CLEAVAGE) 111 4.2.2 SAMPLE HOMOGENEITY
111 4.2.3 PRESSURE-AREA ISOTHERM 112 4.2.4 QUARTZ CRYSTAL NANOBALANCE
113 CONTENTS X 4.2.5 CYTOCHROME P450SCC CRYSTALLIZATION 114 4.2.6 ATOMIC
FORCE MICROSCOPY OF CYTOCHROME MICROCRYSTALS 118 4.3 YET UNRESOLVED
PROTEINS: HUMAN KJNASE CK2A CATALYTIC SUBUNIT 119 4.3.1 PROTEIN KINASE
CK2A CATALYTIC SUBUNIT 119 4.3.2 SAMPLE HOMOGENITY 120 4.3.3
PRESSURE-AREA ISOTHERM 123 4.3.4 QUARTZ CRYSTAL NANOBALANCE 124 4.3.5
HUMAN PROTEIN KINASE CK2CT CRYSTALLIZATION 125 4.3.6 SYNCHROTRON
RADIATION: ANALYSIS, DATA PROCESSING AND STRUCTURE DETERMINATION 128 4.4
CONCLUSIONS 136 5 FROM SCIENCE TECHNOLOGY IN PROTEOMICS 137 5.1
AUTOMATED MASS SPECTROMETRY 138 5.2 BLOMOLECULAR ARRAYS 141 5.2.1 THE
SURFACE PATTERNING 142 5.2.2 THE INSTRUMENTAL LAYOUT AND DATA ANALYSIS
153 5.3 UNSOLVED PROTEIN STRUCTURES OF SCIENTIFIC AND INDUSTRIAL
INTEREST 164 6 FUTURE TRENDS 167 6.1 NOVEL COMPOSITE BIOMATERIALS AND
NANODEVICES 168 6.2 NANOCRYSTALS 172 REFERENCES 175 INDEX 189
|
| any_adam_object | 1 |
| author | Pechkova, Eugenia Nicolini, Claudio |
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| dewey-ones | 572 - Biochemistry |
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| dewey-sort | 3572 16 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Physik Biologie |
| format | Book |
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| id | DE-604.BV017446548 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:18:06Z |
| institution | BVB |
| isbn | 0306479028 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010510672 |
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| owner | DE-703 DE-11 |
| owner_facet | DE-703 DE-11 |
| physical | XI, 190 S. Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Kluwer Academic / Plenum Publ. |
| record_format | marc |
| spelling | Pechkova, Eugenia Verfasser aut Proteomics and nanocrystallography Eugenia Pechkova and Claudio Nicolini New York [u.a.] Kluwer Academic / Plenum Publ. 2003 XI, 190 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Crystallography Nanostructures Protein Conformation Proteins Structure Proteins genetics Proteomics Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 s DE-604 Nicolini, Claudio Verfasser aut GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010510672&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pechkova, Eugenia Nicolini, Claudio Proteomics and nanocrystallography Crystallography Nanostructures Protein Conformation Proteins Structure Proteins genetics Proteomics Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4596545-6 |
| title | Proteomics and nanocrystallography |
| title_auth | Proteomics and nanocrystallography |
| title_exact_search | Proteomics and nanocrystallography |
| title_full | Proteomics and nanocrystallography Eugenia Pechkova and Claudio Nicolini |
| title_fullStr | Proteomics and nanocrystallography Eugenia Pechkova and Claudio Nicolini |
| title_full_unstemmed | Proteomics and nanocrystallography Eugenia Pechkova and Claudio Nicolini |
| title_short | Proteomics and nanocrystallography |
| title_sort | proteomics and nanocrystallography |
| topic | Crystallography Nanostructures Protein Conformation Proteins Structure Proteins genetics Proteomics Proteomanalyse (DE-588)4596545-6 gnd |
| topic_facet | Crystallography Nanostructures Protein Conformation Proteins Structure Proteins genetics Proteomics Proteomanalyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010510672&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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