The localization and characterization of the amino acid permeases in Arabidopsis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English German |
| Published: |
Tübingen, Ernst-Bloch-Str.14
Okumoto
2003
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zugl.: Tübingen, Univ., Diss.,2003 |
| Physical Description: | 53, 26 S.: Ill. |
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MARC
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| 100 | 1 | |a Okumoto, Sakiko |d 1975- |e Verfasser |0 (DE-588)124865550 |4 aut | |
| 245 | 1 | 0 | |a The localization and characterization of the amino acid permeases in Arabidopsis |c von Sakiko Okumoto |
| 264 | 1 | |a Tübingen, Ernst-Bloch-Str.14 |b Okumoto |c 2003 | |
| 300 | |a 53, 26 S.: Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zugl.: Tübingen, Univ., Diss.,2003 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010440525 | |
Record in the Search Index
| _version_ | 1808225728112623616 |
|---|---|
| adam_text |
CONTENTS
PART
1
THESIS
.
1
SUMMARY
.
2
ZUSAMMENFASSUNG
.
3
1.
INTRODUCTION
.
4
1.1
NITROGEN
ACQUISITION
AND
TRANSLOCATION
IN
PLANTS
.
4
1.2
AMINO
ACID
TRANSPORT
IN
THE
PLANT
BODY
.
4
1.3
MOLECULAR
CLONING
OF
PLANT
AMINO
ACID
TRANSPORTERS
.
5
1.4
FUNCTIONAL
CHARACTERIZATION
OF
AAPS
USING
XENOPUS
OOCYTES
AND
YEAST
.
8
1.5
SUBCELLULAR
LOCALIZATION
OF
AMINO
ACID
TRANSPORTERS
IN
PLANTS
.
8
1.6
TISSUE
SPECIFIC
LOCALIZATION
OF
AMINO
ACID
TRANSPORTERS
IN
PLANTS
.
8
1.7
REGULATION
OF
AMINO
ACID
TRANSPORTERS
IN
PLANTS
.
9
1.8
RESEARCH
GOALS
.
9
2.
MATERIALS
.
10
2.1
SYNTHETIC
OLIGONUCLEOTIDES
AND
OLIGOPEPTIDES
.
10
2.2
VECTORS
AND
PLASMIDS
.
10
2.3
BACTERIA
AND
YEAST
STRAINS
.
10
2.4
PLANT
MATERIAL
.
10
2.5
MEDIA
.
10
3.
METHODS
.
10
3.1
CLONING
AND
SEQUENCING
.
11
3.2
CONSTRUCTS
.
11
3.2.1
AAPPROMOTER-GUS
CONSTRUCTS
.
11
3.2.2
GFP
FUSION
CONSTRUCTS
.
11
3.2.3
C-MYCAAP3
CONSTRUCTS
.
12
3.3
BACTERIAL
AND
YEAST
TRANSFORMATION
.
12
3.4
ARABIDOPSIS
TRANSFORMATION
.
13
3.5
PLANT
GROWTH
CONDITIONS
.
13
3.5.1
GREEN
HOUSE
.
13
3.5.2
GROWTH
CABINET
.
13
3.5.3
HYDROPONIC
CULTURE
.
13
3.6
HISTOCHEMICAL
LOCALIZATION
OF
GUS
ACTIVITY
.
14
3.7
METHYLJASMONATE
TREATMENT
.
14
3.8
TRANSIENT
EXPRESSION
IN
PROTOPLASTS
.
14
3.9
MICROSOMAL
PROTEIN
ISOLATION
FROM
PLANTS
AND
CRUDE
MEMBRANE
FRACTIONS
.
14
3.10
PROTEINASE
TREATMENT
OF
YEAST
SPHEROPLASTS
.
14
3.11
RNA
PREPARATION
.
15
3.12RT-PCR
.
15
3.13
RNA
GEL-BIOT
ANALYSIS
.
15
3.14
MICROARRAY
.
15
3.14.1
FLUORESCENT
LABELING
OF
PROBES
.
15
3.14.2
HYBRIDIZATION
OF
MICROARRAYS
.
15
4.
RESULTS
.
17
4.1
EXPRESSION
ANALYSES
USING
AAPS-PROMOTER-GUS
STUDIES
.
17
4.1.1
AAP3
PROMOTER-GUS
STUDIES
.
17
4.1.2
AAP4
PROMOTER-GUS
STUDIES
.
18
4.1.3
AAP5
PROMOTER-GUS
STUDIES
.
18
4.1.4
AAP6
PROMOTER-GUS
STUDIES
.
19
4.1.5
AAP7
PROMOTER-GUS
STUDIES
.
19
4.2
REGULATION
OF
THE
EXPRESSION
OF
AAPS
.
19
4.2.1
THE
REGULATION
OF
AAP3
EXPRESSION
.
19
4.2.2
THE
REGULATION
OF
AAP6
EXPRESSION
.
19
4.3
SUBCELLUIAR
LOCALIZATION
OF
AAPS
.
20
4.3.1
THE
LOCALIZATION
OF
GFP
FUSION
PROTEINS
IN
YEAST
CELLS
.
20
4.3.2
TRANSIENT
EXPRESSION
OF
GFP
FUSION
PROTEINS
IN
PROTOPLASTS
.
22
4.3.3
LOCALIZATION
STUDIES
OF
C-MYCAAP3
.
22
4.4
THE
TOPOLOGY
OF
AAP3
PROTEIN
.
24
4.5
ANALYSIS
OF
AAP3
AND
AAP6
T-DNA
INSERTION
LINES
.
25
4.5.1
ISOLATION
AND
ANALYSIS
OF
AAP3-L
AND
AAP3-2
LINES
.
25
4.5.2
ISOLATION
AND
ANALYSIS
OF
THE
AAP6-L
LINE
.
26
4.5.3
MICROARRAY
OF
AAP6-L
PLANTS
.
26
5.
DISCUSSION
.
29
5.1
SUBCELLUIAR
LOCALIZATION
OF
AAPS
IN
PLANT
.
29
5.2
DISTINCT
EXPRESSION
PATTERNS
OF
AAPS
AND
THEIR
FUNCTION
.
30
5.2.1
THE
ROOT-SPECIFIC
ROLE
OF
AAP3
.
30
5.2.2
EXPRESSION
OF
AAP4
IN
THE
PHLOEM
TISSUE
OF
LEAVES
.
30
5.2.3
THE
EXPRESSION
OF
AAP5
IN
ARABIDOPSIS
.
31
5.2.4
THE
ROLE
OF
AAP6
IN
XYLEM
PARENCHYMA
CELLS
.
31
5.3
REGULATION
OF
AMINO
ACID
TRANSPORTERS
IN
PLANTS
.
31
5.4
THE
TOPOLOGY
OF
AAPS
.
32
5.5
FUNCTIONAL
ANALYSIS
OF
AAPS
USING
T-DNA
INSERTION
LINES
.
33
5.6
THE
ROLE
OF
ARABIDOPSIS
AAPS
IN
PLANTA
.
33
5.6.1
AMINO
ACID
TRANSPORT
IN
THE
ROOT
.
33
5.6.2
AMINO
ACID
TRANSPORT
IN
THE
LEAF
.
33
5.6.3
AMINO
ACID
TRANSPORT
DURING
REPRODUCTION
.
34
5.6.4
AMINO
ACID
TRANSPORT
BETWEEN
XYLEM
AND
PHLOEM
.
34
6.
REFERENCES
.
38
7.
ACKNOWLEDGEMENTS
.
45
8.
APPENDICES
.
46
8.1
LIST
OF
PRIMERS
.
46
8.2
ABBREVIATIONS
.
48
8.3
MAP
OF
CONSTRUCTS
.
50
CONTRIBUTION
TO
THE
MANUSCRIPTS
.
52
LEBENSLAUF
53 |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV017317080 |
| illustrated | Not Illustrated |
| indexdate | 2024-08-24T00:13:53Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010440525 |
| oclc_num | 76580747 |
| open_access_boolean | |
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| owner_facet | DE-29T DE-12 |
| physical | 53, 26 S.: Ill. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Okumoto |
| record_format | marc |
| spelling | Okumoto, Sakiko 1975- Verfasser (DE-588)124865550 aut The localization and characterization of the amino acid permeases in Arabidopsis von Sakiko Okumoto Tübingen, Ernst-Bloch-Str.14 Okumoto 2003 53, 26 S.: Ill. txt rdacontent n rdamedia nc rdacarrier Zugl.: Tübingen, Univ., Diss.,2003 Permeasen (DE-588)4367251-6 gnd rswk-swf Aminosäuren (DE-588)4142205-3 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Aminosäuren (DE-588)4142205-3 s Permeasen (DE-588)4367251-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010440525&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Okumoto, Sakiko 1975- The localization and characterization of the amino acid permeases in Arabidopsis Permeasen (DE-588)4367251-6 gnd Aminosäuren (DE-588)4142205-3 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4367251-6 (DE-588)4142205-3 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | The localization and characterization of the amino acid permeases in Arabidopsis |
| title_auth | The localization and characterization of the amino acid permeases in Arabidopsis |
| title_exact_search | The localization and characterization of the amino acid permeases in Arabidopsis |
| title_full | The localization and characterization of the amino acid permeases in Arabidopsis von Sakiko Okumoto |
| title_fullStr | The localization and characterization of the amino acid permeases in Arabidopsis von Sakiko Okumoto |
| title_full_unstemmed | The localization and characterization of the amino acid permeases in Arabidopsis von Sakiko Okumoto |
| title_short | The localization and characterization of the amino acid permeases in Arabidopsis |
| title_sort | the localization and characterization of the amino acid permeases in arabidopsis |
| topic | Permeasen (DE-588)4367251-6 gnd Aminosäuren (DE-588)4142205-3 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Permeasen Aminosäuren Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010440525&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT okumotosakiko thelocalizationandcharacterizationoftheaminoacidpermeasesinarabidopsis |