Methods in modern biophysics:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin [u.a.]
Springer
2004
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 254 S. graph. Darst. |
| ISBN: | 3540012974 |
Staff View
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| 245 | 1 | 0 | |a Methods in modern biophysics |c Bengt Nölting |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2004 | |
| 300 | |a XIV, 254 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Biophysics |x Methodology | |
| 650 | 4 | |a Biophysics |x methods | |
| 650 | 4 | |a Microscopy, Electron |x methods | |
| 650 | 4 | |a Spectrum Analysis |x methods | |
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Record in the Search Index
| _version_ | 1808044740281630720 |
|---|---|
| adam_text |
CONTENTS
1
THE
THREE-DIMENSIONAL
STRUCTURE
OF
PROTEINS
.
1
1.1
STRUCTURE
OF
THE
NATIVE
STATE
.
1
1.2
PROTEIN
FOLDING
TRANSITION
STATES
.
9
1.3
STRUCTURAL
DETERMINANTS
OF
THE
FOLDING
RATE
CONSTANTS
.
12
1.4
SUPPORT
OF
STRUCTURE
DETERMINATION
BY
PROTEIN
FOLDING
SIMULATIONS
.
20
2
LIQUID
CHROMATOGRAPHY
OF
BIOMOLECULES
.
23
2.1
ION
EXCHANGE
CHROMATOGRAPHY
.
23
2.2
GEL
FILTRATION
CHROMATOGRAPHY
.
28
2.3
AFFINITY
CHROMATOGRAPHY
.
31
2.4 COUNTER-CURRENT
CHROMATOGRAPHY
AND
ULTRAFILTRATION
.
33
3
MASS
SPECTROMETRY
.
37
3.1
PRINCIPLES
OF
OPERATION
AND
TYPES
OF
SPECTROMETERS
.
37
3.1.1
SECTOR
MASS
SPECTROMETER
.
38
3.1.2
QUADRUPOLE
MASS
SPECTROMETER
.
39
3.1.3
ION
TRAP
MASS
SPECTROMETER
.
39
3.1.4
TIME-OF-FLIGHT
MASS
SPECTROMETER
.
40
3.1.5
FOURIER
TRANSFORM
MASS
SPECTROMETER
.
43
3.1.6
IONIZATION,
ION
TRANSPORT
AND
ION
DETECTION
.
44
3.1.7
ION
FRAGMENTATION
.
45
3.1.8
COMBINATION
WITH
CHROMATOGRAPHIC
METHODS
.
46
3.2
BIOPHYSICAL
APPLICATIONS
.
49
4
X-RAY
STRUCTURAL
ANALYSIS
.
59
4.1
FOURIER
TRANSFORM
AND
X-RAY
CRYSTALLOGRAPHY
.
59
4.1.1
FOURIER
TRANSFORM
.
59
4.1.2
PROTEIN
X-RAY
CRYSTALLOGRAPHY
.
69
4.1.2.1
OVERVIEW
.
69
4.1.2.2
PRODUCTION
OF
SUITABLE
CRYSTALS
.
69
4.1.2.3
ACQUISITION
OF
THE
DIFFRACTION
PATTERN
.
71
X
CONTENTS
4.1.2.4
DETERMINATION
OF
THE
PHASES:
HEAVY
ATOM
REPLACEMENT
.
76
4.1.2.5
CALCULATION
OF
THE
ELECTRON
DENSITY
AND
REFINEMENT
.
83
4.1.2.6
CRYOCRYSTALLOGRAPHY
AND
TIME-RESOLVED
CRYSTALLOGRAPHY
.
84
4.2
X-RAY
SCATTERING
.
85
4.2.1
SMALL
ANGLE
X-RAY
SCATTERING
(SAXS)
.
85
4.2.2
X-RAY
BACKSCATTERING
.
88
5
PROTEIN
INFRARED
SPECTROSCOPY
.
91
5.1
SPECTROMETERS
AND
DEVICES
.
92
5.1.1
SCANNING
INFRARED
SPECTROMETERS
.
92
5.1.2
FOURIER
TRANSFORM
INFRARED
(FTIR)
SPECTROMETERS
.
92
5.1.3
LIDAR,
OPTICAL
COHERENCE
TOMOGRAPHY,
ATTENUATED
TOTAL
.
96
REFLECTION
AND
IR
MICROSCOPES
5.2
APPLICATIONS
.
102
6
ELECTRON
MICROSCOPY
.
107
6.1
TRANSMISSION
ELECTRON
MICROSCOPE
(TEM)
.
107
6.1.1
GENERAL
DESIGN
.
107
6.1.2
RESOLUTION
.
109
6.1.3
ELECTRON
SOURCES
.
110
6.1.4
TEM
GRIDS
.
112
6.1.5
ELECTRON
LENSES
.
112
6.1.6
ELECTRON-SAMPLE
INTERACTIONS
AND
ELECTRON
SPECTROSCOPY
.
115
6.1.7
EXAMPLES
OF
BIOPHYSICAL
APPLICATIONS
.
117
6.2
SCANNING
TRANSMISSION
ELECTRON
MICROSCOPE
(STEM)
.
118
7
SCANNING
PROBE
MICROSCOPY
.
121
7.1
ATOMIC
FORCE
MICROSCOPE
(AFM)
.
121
7.2
SCANNING
TUNNELING
MICROSCOPE
(STM)
.
133
7.3
SCANNING
NEARFIELD
OPTICAL
MICROSCOPE
(SNOM)
.
135
7.3.1
OVERCOMING
THE
CLASSICAL
LIMITS
OF
OPTICS
.
135
7.3.2
DESIGN
OF
THE
SUBWAVELENGTH
APERTURE
.
138
7.3.3
EXAMPLES
OF
SNOM
APPLICATIONS
.
142
7.4
SCANNING
ION
CONDUCTANCE
MICROSCOPE,
SCANNING
THERMAL
.
143
MICROSCOPE
AND
FURTHER
SCANNING
PROBE
MICROSCOPES
8
BIOPHYSICAL
NANOTECHNOLOGY
.
147
8.1
FORCE
MEASUREMENTS
IN
SINGLE
PROTEIN
MOLECULES
.
147
CONTENTS
XI
8.2
FORCE
MEASUREMENTS
IN
A
SINGLE
POLYMERASE-DNA
COMPLEX
.
150
8.3
MOLECULAR
RECOGNITION
.
152
8.4
PROTEIN
NANOARRAYS
AND
PROTEIN
ENGINEERING
.
155
8.5
STUDY
AND
MANIPULATION
OF
PROTEIN
CRYSTAL
GROWTH
.
158
8.6
NANOPIPETTES,
MOLECULAR
DIODES,
SELF-ASSEMBLED
NANOTRANSISTORS,
.
159
NANOPARTICLE-MEDIATED
TRANSFECTION
AND
FURTHER
BIOPHYSICAL
NANOTECHNOLOGIES
9
PROTEOMICS:
HIGH
THROUGHPUT
PROTEIN
FUNCTIONAL
ANALYSIS
.
165
9.1
TARGET
DISCOVERY
.
166
9.2
INTERACTION
PROTEOMICS
.
168
9.3
CHEMICAL
PROTEOMICS
.
172
9.4
LAB-ON-A-CHIP
TECHNOLOGY
AND
MASS-SPECTROMETRIC
ARRAY
SCANNERS
.
173
9.5
STRUCTURAL
PROTEOMICS
.
174
10
ION
MOBILITY
SPECTROMETRY
.
175
10.1
GENERAL
DESIGN
OF
SPECTROMETERS
.
175
10.2
RESOLUTION
AND
SENSITIVITY
.
180
10.3
IMS-BASED
"
SNIFFERS
"
.
183
10.4
DESIGN
DETAILS
.
184
10.5
DETECTION
OF
BIOLOGICAL
AGENTS
.
193
11
MICROWAVE
AUDITORY
EFFECTS
AND
THE
THEORETICAL
CONCEPT
OF
THOUGHT
.
197
TRANSMISSION
TECHNOLOGY
11.1
THEORETICAL
CONCEPT
.
197
11.1.1
BACKGROUND
OF
THE
CONCEPT
OF
THOUGHT
TRANSMISSION
TECHNOLOGY
.
198
11.1.2
DESCRIPTION
OF
THE
TECHNOLOGY
.
199
11.1.2.1
FREQUENCIES
.
200
11.1.2.2
RADIATION
SOURCES
.
201
11.2
EXAMPLES
OF
POTENTIAL
APPLICATIONS
.
202
11.2.1
AN
AUTOMATED
DEVICE
.
202
11.2.2
A
SMALL
HANDHELD
DEVICE
.
203
11.2.3
BIOMEDICAL
MICROWAVE
AUDITORY
AND
THOUGHT
TRANSMISSIONS
.
204
12
CONCLUSIONS
.
211
REFERENCES
.
213
INDEX
.
245 |
| any_adam_object | 1 |
| author | Nölting, Bengt 1962- |
| author_GND | (DE-588)113522673 |
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| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 2050 |
| classification_tum | CHE 802f PHY 820f |
| ctrlnum | (OCoLC)54483972 (DE-599)BVBBV017258873 |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.4 |
| dewey-search | 571.4 |
| dewey-sort | 3571.4 |
| dewey-tens | 570 - Biology |
| discipline | Physik Biologie Chemie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-08-22T00:17:11Z |
| institution | BVB |
| isbn | 3540012974 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010402814 |
| oclc_num | 54483972 |
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| owner | DE-29 DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-20 DE-703 DE-M347 DE-11 DE-188 |
| owner_facet | DE-29 DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-20 DE-703 DE-M347 DE-11 DE-188 |
| physical | XIV, 254 S. graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Springer |
| record_format | marc |
| spelling | Nölting, Bengt 1962- Verfasser (DE-588)113522673 aut Methods in modern biophysics Bengt Nölting Berlin [u.a.] Springer 2004 XIV, 254 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biophysics Methodology Biophysics methods Microscopy, Electron methods Spectrum Analysis methods Methode (DE-588)4038971-6 gnd rswk-swf Biophysik (DE-588)4006891-2 gnd rswk-swf Biophysik (DE-588)4006891-2 s Methode (DE-588)4038971-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010402814&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nölting, Bengt 1962- Methods in modern biophysics Biophysics Methodology Biophysics methods Microscopy, Electron methods Spectrum Analysis methods Methode (DE-588)4038971-6 gnd Biophysik (DE-588)4006891-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4006891-2 |
| title | Methods in modern biophysics |
| title_auth | Methods in modern biophysics |
| title_exact_search | Methods in modern biophysics |
| title_full | Methods in modern biophysics Bengt Nölting |
| title_fullStr | Methods in modern biophysics Bengt Nölting |
| title_full_unstemmed | Methods in modern biophysics Bengt Nölting |
| title_short | Methods in modern biophysics |
| title_sort | methods in modern biophysics |
| topic | Biophysics Methodology Biophysics methods Microscopy, Electron methods Spectrum Analysis methods Methode (DE-588)4038971-6 gnd Biophysik (DE-588)4006891-2 gnd |
| topic_facet | Biophysics Methodology Biophysics methods Microscopy, Electron methods Spectrum Analysis methods Methode Biophysik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010402814&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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