Tetracycline aptamer mediated translational control:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 83 S. . Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV017184576 | ||
| 003 | DE-604 | ||
| 005 | 20030821 | ||
| 007 | t | ||
| 008 | 030602s2003 m||| 00||| eng d | ||
| 016 | 7 | |a 967927048 |2 DE-101 | |
| 035 | |a (OCoLC)249594692 | ||
| 035 | |a (DE-599)BVBBV017184576 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng |a ger | |
| 049 | |a DE-29 |a DE-29T |a DE-12 |a DE-355 |a DE-83 |a DE-188 | ||
| 084 | |a WD 5355 |0 (DE-625)148201: |2 rvk | ||
| 100 | 1 | |a Hanson, Shane |d 1972- |e Verfasser |0 (DE-588)124757391 |4 aut | |
| 245 | 1 | 0 | |a Tetracycline aptamer mediated translational control |c von Shane Hanson |
| 264 | 1 | |c 2003 | |
| 300 | |a 83 S. . Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2003 | ||
| 650 | 4 | |a Translationskontrolle - Aptamer - Tetracycline | |
| 650 | 0 | 7 | |a Tetracycline |0 (DE-588)4078215-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Aptamer |0 (DE-588)4441960-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Translationskontrolle |0 (DE-588)4379556-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Translationskontrolle |0 (DE-588)4379556-0 |D s |
| 689 | 0 | 1 | |a Aptamer |0 (DE-588)4441960-0 |D s |
| 689 | 0 | 2 | |a Tetracycline |0 (DE-588)4078215-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010355944&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010355944 | |
Datensatz im Suchindex
| _version_ | 1807682889359294464 |
|---|---|
| adam_text |
INDEX
I
1.
ZUSAMMENFASSUNG
/
SUMMARY
1
2.
INTRODUCTION
2.1
TARGETING
TRANSLATION
INITIATION
2
2.1.1
'
RIBOSWITCHES
'
-
RNA
AS
A
MOLECULAR
SENSOR
2
2.1.2
PATHWAYS
OF
TRANSLATION
INITIATION
IN
EUKARYOTES
3
2.I.2.I.
FACTORS
AFFECTING
TRANSLATION
INITIATION
AND
MRNA
TURNOVER
3
2.1.2.2
ROLE
OF
UPSTREAM
OPEN
READING
FRAMES
4
2.2
RNA
AS
A
DRUG
TARGET
5
2.2.1
SMALL
MOLECULE
DRUGS
THAT
TARGET
RNA
5
2.2.1.1
THE
AMINOGLYCOSIDES
5
2.2.1.2
THE
TETRACYCLINES
6
2.2.1.3
OTHER
ANTIBIOTICS
8
2.2.2
RNA
APTAMERS
9
23
CONDITIONALLY
REGULATED
GENE
EXPRESSION
SYSTEMS
10
2.3.1
TRANSCRIPTIONAL
REGULATION
SYSTEMS
11
2.3.2
TRANSLATIONAL
REGULATION
SYSTEMS
12
2.3.1.1
ANTI-SENSE,
RNAI
AND
RIBOZYME
TECHNOLOGIES
12
2.3.1.2
APTAMER
MEDIATED
TRANSLATIONAL
CONTROL
13
2.4
AIM
OF
THIS
WORK
14
3
RESULTS
3.1
ELUCIDATION
OF
STRUCTURE-FUNCTION
RELATIONSHIP
OF
APTAMER-TC
COMPLEX
15
3.1.1
ANALYSIS
OF
TC-BINDING
APTAMERS
FOR
THEIR
ABILITY
TO
REGULATE
GENE
EXPRESSION
15
3.1.2
BIOCHEMICAL
CHARACTERISATION
OF
TC-BINDING
APTAMERS
16
3.1.2.1
ENZYMATIC
PROBING
17
3.1.2.2
CHEMICAL
PROBING
20
3.1.2.3
THERMAL
DENATURATION
OF
TC-APTAMER
32SH
24
3.1.3
INVESTIGATING
STRUCTURE-FUNCTION
RELATIONSHIPS
BY
SITE-DIRECTED
MUTAGENESIS
25
3.2
IN
VIVO
SCREEN
FOR
APTAMERS
WITH
IMPROVED
REGULATORY
PROPERTIES
27
3.3
CHARACTERISATION
OF
TRANSLATIONAL
REGULATION
IN
YEAST
31
3.3.1
EFFECT
OF
POSITION
OF
APTAMER
IN
THE
5
'
UTR
31
3.3.2
EFFECT
OF
CFS-ACTING
ELEMENTS
ON
REGULATORY
PROPERTIES
AND
MRNA
LEVELS
32
INDEX
II
3.4
ELUCIDATION
OF
THE
MECHANISM
OF
APTAMER
MEDIATED
TRANSLATION
INHIBITION
35
3.4.1
TRANSLATIONAL
INHIBITION
IN
CELL-FREE
EXTRACTS
35
3.4.2
KINETICS
OF
TC
BINDING
TO
THE
APTAMER
CONTAINING
RNRNA
38
3.4.3
POLYSOMAL
GRADIENT
ANALYSIS
OF
TC-APTAMER
BEARING
MRNAS
38
3.5
TC-APTAMER
MEDIATED
TRANSLATIONAL
REGULATION
IN
HIGHER
EUKARYOTES
40
3.5.1.
TRANSIENT
TRANSFECTIONS
IN
HELA
CELL
LINE
40
3.5.2.
IN
VITRO
TRANSLATIONS
USING
WHEAT
GERM
EXTRACTS
(WGE)
43
4
DISCUSSION
4.1
IMPLICATIONS
OF
STRUCTURE-FUNCTION
RELATIONSHIP
OF
THE
APTAMER-TC
COMPLEX
45
4.1.1
MAPPING
THE
APTAMER
STRUCTURE
45
4.1.2
DEFINING
A
TC-BINDING
POCKET
46
4.1.3
IMPLICATIONS
OF
A
COMPOSITE
BINDING
SITE
48
4.2
DEVELOPMENT
OF
TC-APTAMER
BASED
TRANSLATIONAL
REGULATION
SYSTEMS
50
4.2.1
INFLUENCE
OF
APTAMER
INSERTION
ON
BASAL
TRANSLATION
50
4.2.2
TC-APTAMER
MEDIATED
INHIBITION
OF
TRANSLATION
52
4.2.3
INTERDEPENDENCE
OF
APTAMER
INSERTION
AND
TRANSLATION
INHIBITION
ON
MRNA
LEVELS
53
4.3
MECHANISM
OF
TC-DEPENDENT
TRANSLATIONAL
REGULATION
54
4.4
OUTLOOK
56
5
MATERIALS
AND
METHODS
5.1
MATERIALS
57
5.2
MEDIA,
BUFFERS
AND
SOLUTIONS
62
5.2.1
MEDIA
62
5.2.2
BUFFERS
AND
SOLUTIONS
63
5.2.2.1
AGAROSE
GEL
ELECTROPHORESIS
63
5.2.2.2
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
63
5.2.2.3
RNA
(DENATURING)
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
63
5.2.2.4
BIOCHEMICAL
ANALYSIS
OF
RNA
63
5.2.2.5
RNA
ISOLATION
AND
NORTHERN
HYBRIDIZATION
64
5.2.2.6
IN
VITRO
TRANSLATION
AND
SUCROSE
GRADIENT
ANALYSIS
64
5.2.2.7
TRANSIENT
TRANSFECTIONS
65
5.2.2.8
THERMAL
DENATURATION
OF
RNA
65
INDEX
III
53
GENERAL
METHODS
53.1
BIOCHEMICAL
ANALYSIS
66
53.1.1
PREPARATION
AND
PURIFICATION
OF
RNA
66
53.1.2
CHEMICAL
MODIFICATION
ASSAYS
67
5.3.13
PRIMER
EXTENSION
AND
REVERSE
TRANSCRIPTION
67
5.3.1.4
ENZYMATIC
PROBING
ASSAY
68
53.1.5
THERMAL
DENATURATION
OF
RNA
68
5.3.2
YEAST
PROTOCOLS
68
5.32.1
GFP
MEASUREMENTS
68
53.2.2
LUCIFERASE
ASSAY
69
5.3.23
PROTEIN
ESTIMATION
69
53.2.4
RNA
ISOLATION
69
53.2.5
NORTHERN
BLOT
ANALYSIS
69
53.2.6
PREPARATION
OF
YEAST
CELL-FREE
EXTRACTS
70
53.2.7
IN
VITRO
TRANSCRIPTION
AND
TRANSLATION
70
53.2.8
IN
VITRO
POLYSOMAL
SUCROSE
GRADIENTS
71
5.3.3
CELL
CULTURE
71
53.3.1
GENERAL
PROTOCOLS
71
5.33.2
TRANSIENT
TRANSFECTIONS
AND
ANALYSIS
OF
REPORTER
GENE
ACTIVITIES
72
6
LITERATURE
73
7
ABBREVIATION
INDEX
82 |
| any_adam_object | 1 |
| author | Hanson, Shane 1972- |
| author_GND | (DE-588)124757391 |
| author_facet | Hanson, Shane 1972- |
| author_role | aut |
| author_sort | Hanson, Shane 1972- |
| author_variant | s h sh |
| building | Verbundindex |
| bvnumber | BV017184576 |
| classification_rvk | WD 5355 |
| ctrlnum | (OCoLC)249594692 (DE-599)BVBBV017184576 |
| discipline | Biologie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV017184576</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20030821</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">030602s2003 m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">967927048</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)249594692</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV017184576</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield><subfield code="a">ger</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-83</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5355</subfield><subfield code="0">(DE-625)148201:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hanson, Shane</subfield><subfield code="d">1972-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)124757391</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Tetracycline aptamer mediated translational control</subfield><subfield code="c">von Shane Hanson</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">83 S. . Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Erlangen-Nürnberg, Univ., Diss., 2003</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Translationskontrolle - Aptamer - Tetracycline</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Tetracycline</subfield><subfield code="0">(DE-588)4078215-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Aptamer</subfield><subfield code="0">(DE-588)4441960-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Translationskontrolle</subfield><subfield code="0">(DE-588)4379556-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Translationskontrolle</subfield><subfield code="0">(DE-588)4379556-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Aptamer</subfield><subfield code="0">(DE-588)4441960-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Tetracycline</subfield><subfield code="0">(DE-588)4078215-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010355944&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-010355944</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017184576 |
| illustrated | Not Illustrated |
| indexdate | 2024-08-18T00:25:42Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010355944 |
| oclc_num | 249594692 |
| open_access_boolean | |
| owner | DE-29 DE-29T DE-12 DE-355 DE-BY-UBR DE-83 DE-188 |
| owner_facet | DE-29 DE-29T DE-12 DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | 83 S. . Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| record_format | marc |
| spelling | Hanson, Shane 1972- Verfasser (DE-588)124757391 aut Tetracycline aptamer mediated translational control von Shane Hanson 2003 83 S. . Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2003 Translationskontrolle - Aptamer - Tetracycline Tetracycline (DE-588)4078215-3 gnd rswk-swf Aptamer (DE-588)4441960-0 gnd rswk-swf Translationskontrolle (DE-588)4379556-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Translationskontrolle (DE-588)4379556-0 s Aptamer (DE-588)4441960-0 s Tetracycline (DE-588)4078215-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010355944&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hanson, Shane 1972- Tetracycline aptamer mediated translational control Translationskontrolle - Aptamer - Tetracycline Tetracycline (DE-588)4078215-3 gnd Aptamer (DE-588)4441960-0 gnd Translationskontrolle (DE-588)4379556-0 gnd |
| subject_GND | (DE-588)4078215-3 (DE-588)4441960-0 (DE-588)4379556-0 (DE-588)4113937-9 |
| title | Tetracycline aptamer mediated translational control |
| title_auth | Tetracycline aptamer mediated translational control |
| title_exact_search | Tetracycline aptamer mediated translational control |
| title_full | Tetracycline aptamer mediated translational control von Shane Hanson |
| title_fullStr | Tetracycline aptamer mediated translational control von Shane Hanson |
| title_full_unstemmed | Tetracycline aptamer mediated translational control von Shane Hanson |
| title_short | Tetracycline aptamer mediated translational control |
| title_sort | tetracycline aptamer mediated translational control |
| topic | Translationskontrolle - Aptamer - Tetracycline Tetracycline (DE-588)4078215-3 gnd Aptamer (DE-588)4441960-0 gnd Translationskontrolle (DE-588)4379556-0 gnd |
| topic_facet | Translationskontrolle - Aptamer - Tetracycline Tetracycline Aptamer Translationskontrolle Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010355944&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hansonshane tetracyclineaptamermediatedtranslationalcontrol |