Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2002
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Köln, Univ., Diss., 2002 |
| Physical Description: | X, 133 S. graph. Darst. |
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MARC
| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV017074510 | ||
| 003 | DE-604 | ||
| 005 | 20030417 | ||
| 007 | t | ||
| 008 | 030417s2002 d||| m||| 00||| eng d | ||
| 016 | 7 | |a 965884767 |2 DE-101 | |
| 035 | |a (OCoLC)51713003 | ||
| 035 | |a (DE-599)BVBBV017074510 | ||
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| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a LU, Zhongju |d 1972- |e Verfasser |0 (DE-588)124286895 |4 aut | |
| 245 | 1 | 0 | |a Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese |c von Zhongju Lu |
| 264 | 1 | |c 2002 | |
| 300 | |a X, 133 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Köln, Univ., Diss., 2002 | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010300892&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010300892 | |
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| _version_ | 1807593316937629696 |
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| adam_text |
CONTENTS
1.
INTRODUCTION
.
1
1.1
CELLULAR
MODELS
FOR
THE
INVESTIGATION
OF
IONIC
CHANNELS
DURING
CARDIOMYOGENESIS
.
1
1.1.1
THE
MURINE
EMBRYONIC
STEM
(ES)
CELL
IN
VITRO
MODEL
.
2
1.1.2
MURINE
EMBRYONIC
CARDIOMYOCYTES
AS
AN
IN
VIVO
MODEL
.
4
1.2
IONIC
BASIS
OF
UNDERLYING
THE
CONTROL
OF
ELECTRICAL
EXCITABILITY
IN
CARDIOMYOCYTES
.
5
1.3
EXPRESSION,
FUNCTION
AND
ASSOCIATED
MOLECULAR
PROPERTIES
OF
CARDIAC
POTASSIUM
CHANNELS
.
7
1.4.
CELLULAR
MECHANISMS
RESPONSIBLE
FOR
THE
GENERATION
OF
SPONTANEOUS
ELECTRICAL
ACTIVITY
.
9
1.5.
FUNCTIONAL
ANALYSIS
OF
CARDIAC
SUBTYPES
DURING
MOUSE
EMBRYONIC
DEVELOPMENT
.
10
1.5.1.
EXPRESSION
PATTERN
OF
MHC
GENES
DURING
EMBRYONIC
DEVELOPMENT
.
11
1.5.2.
EXPRESSION
PATTERN
OF
MLC2V
GENE
IN
THE
VENTRICULAR
CELLS
DURING
EMBRYONIC
DEVELOPMENT
.
12
2.
AIMS
OF
PRESENT
STUDY
.
13
3.
MATERIALS
AND
METHODS
.
14
3.1
ES
CELL
CULTURE
AND
DIFFERENTIATION
PROCEDURE
.
14
3.2.
VECTOR
CONSTRUCTION,
TRANSFECTION
AND
SELECTION
PROCEDURE
.
16
3.3
PREPARATION
OF
ISOLATED
CARDIOMYOCYTES
.
17
3.3.1
PREPARATION
OF
ISOLATED
ES-CELL
DERIVED
CARDIOMYOCYTES
.
17
3.3.2.
PREPARATION
OF
ISOLATED
MURINE
EMBRYONIC
CARDIOMYOCYTES
.
18
3.4.
ELECTROPHYSIOLOGICAL
MEASUREMENTS
.
19
3.4.1
PROTOCOLS
FOR
THE
RECORDING
OF
OUTWARD
K
+
CURRENTS
(I
K
)
AND
INWARD
K
+
CURRENT
(I
KI
)
.
19
3.4.2
PROTOCOLS
FOR
THE
RECORDINGS
OF
HYPERPOLARIZATION
ACTIVATED
NONE
SELECTIVE
CATION
CURRENTS
(IF)
.
21
3.4.3
PROTOCOLS
FOR
THE
SIMULTANEOUS
RECORDING
OF
I
F
AND
L
TYPE
CALCIUM
CURRENTS
(I
CA
,L)
.
21
3.5
GLASS
PIPETTES
AND
RECORDING
SOLUTIONS
.
22
3.6
COMBINED
IMAGING
AND
ELECTROPHYSIOLOGICAL
RECORDING
SYSTEM
.
23
3.7.
IMMUNOHISTOCHEMISTRY
.
25
3.8.
SOURCES
OF
TEST
SUBSTANCES
.
25
4.
RESULTS
.
26
4.1
FUNCTIONAL
EXPRESSION
OF
OUTWARD
K
+
CURRENTS
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
.
26
4.1.1
EXPRESSION
OF
OUTWARD
CURRENTS
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
DURING
DEVELOPMENT
.
26
4.1.2
PHARMACOLOGICAL
DISSECTION
OF
MAIN
COMPONENTS
OF
FAST
ACTIVATING
OUTWARD
RECTIFIER
CURRENTS
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
.
27
4.1.3
EXPRESSION
AND
CURRENT
DENSITY
OF
THREE
OUTWARD
RECTIFIER
CURRENT
COMPONENTS
OF
OUTWARD
POTASSIUM
CURRENTS
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
DURING
DEVELOPMENT
.
30
4.1.4
BIOPHYSICAL
PROPERTIES
OF
I
T0
AND
I
K
,
SUS
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
32
4.1.5
EFFECTS
OF
4-AP
ON
THE
APS
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
DURING
DEVELOPMENT.
.
35
4.1.6
THE
FUNCTIONAL
EXPRESSION
OF
I
K
I
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
38
4.2
CELLULAR
MECHANISMS
RESPONSIBLE
FOR
THE
GENERATION
OF
CARDIAC
PACEMAKING
ACTIVITY
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
41
4.2.1
THE
BIOPHYSICAL
PROPERTIES
OF
IF
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
41
4.2.2
FUNCTIONAL
EXPRESSION
OF
IF
IN
ES
CELL-DERIVED
CARDIOMYOCYTES
AT
DIFFERENT
STAGES
OF
DEVELOPMENT
.
43
4.2.3
IF
IS
STIMULATED
BY
PKA
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
46
4.2.4
IF
IS
STIMULATED
BY
PKA
PHOSPHORYLATION
VERSUS
DIRECT
CAMP
MODULATION
IN
EDS
ES
CELL-DERIVED
CARDIOMYOCYTES
.
46
4.3
IDENTIFICATION,
SEPARATION
AND
FUNCTIONAL
ANALYSIS
OF
EARLY
CARDIAC
PHENOTYPES
USING
A
CARDIAC
SPECIFIC
PROMOTER
DRIVING
EXPRESSION
OF
A
LIVE
REPORTER
GENE
.
49
4.3.1
CARDIAC
SPECIFIC
EXPRESSION
OF
A-MHC
PROMOTER-DRIVING
EGFP
REPORTER
GENE
DURING
THE
DEVELOPMENT
OF
ES
CELL-DERIVED
CARDIOMYOCYTES
.
49
4.3.2
SPECIFICITY
OF
EGFP
EXPRESSION
IN
AMHC-EGFP
POSITIVE
CELLS
.
51
4.3.3.
DIFFERENTIAL
EGFP
INTENSITY
DISTRIBUTION
AMONG
DIFFERENT
POPULATION
OF
AMHC-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
AT
EDS
STAGE
.
51
4.3.4
IDENTIFICATION
OF
ATRIAL/PACEMAKER
CELLS
IN
EDS
AMHC-EGFP
POSITIVE
CELLS
.
51
4.3.5.
BIOPHYSICAL
AND
PHARMACOLOGICAL
CHARACTERISTICS
OF
IF
IN
AMHC-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
AT
EDS
STAGE
.
55
4.3.6.THE
EXPRESSION
OF
I
K)
IN
AMHC-EGFP
POSITIVE
CARDIOMYOCYTES
AT
EDS
STAGE
.
58
4.3.7
P-ADRENERGIC
MODULATION
OF
SPONTANEOUS
APS
IN
AMHC-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
AT
EDS
STAGE
.
60
4.3.8.
MUSCARINIC
MODULATION
OF
SPONTANEOUS
APS
IN
AMHC-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
AT
EDS
STAGE
.
60
4.3.9
IDENTIFICATION
AND
FUNCTIONAL
ANALYSIS
OF
EARLY
VENTRICULAR
CELLS
USING
A
CARDIAC
SPECIFIC
PROMOTER
MLC2V
DRIVING
EGFP
REPORTER
GENE
.
63
4.3.9.1
DISTRIBUTION
OF
CARDIAC
PHENOTYPES
AMONG
MLC2V-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
DURING
EMBRYONIC
DEVELOPMENT
.
63
4.3.9.2
HORMONAL
MODULATION
OF
SPONTANEOUS
APS
IN
MLC2V-EGFP
POSITIVE
VENTRICULAR
CEIL
AT
EDS
STAGE
.
63
4.4
IDENTIFICATION
AND
FUNCTIONAL
ANALYSIS
OF
THE
EARLY
VENTRICULAR
CELLS
USING
A
CARDIAC
SPECIFIC
PROMOTER
MLC2V
DRIVEN
EGFP
REPORTER
GENE
.
66
4.4.1.
DISTRIBUTION
OF
CARDIAC
PHENOTYPES
AMONG
MLC2V-EGFP
POSITIVE
ES
CELL-DERIVED
CARDIOMYOCYTES
DURING
EMBRYONIC
DEVELOPMENT
.
66
4.4.2
HORMONAL
MODULATION
OF
SPONTANEOUS
APS
IN
THE
MLC2V-EGFP
POSITIVE
VENTRICULAR
CELL
AT
THE
EDS
STAGE
.
66
4.5
FUNCTIONAL
ANALYSIS
OF
IF
AND
ICA,L
CURRENT
IN
THE
EARLY
MURINE
EMBRYONIC
CARDIOMYOCYTES
.
69
4.5.1
IDENTIFICATION
OF
EARLY
MURINE
EMBRYONIC
CARDIOMYOCYTES
.
69
4.5.2
HORMONAL
MODULATION
OF
SPONTANEOUS
APS
IN
THE
MURINE
EMBRYONIC
CARDIOMYOCYTES
AT
THE
EARLY
DEVELOPMENTAL
STAGE
.
71
4.5.3
IDENTIFICATION
OF
IF
IN
THE
EARLY
MURINE
EMBRYONIC
ATRIAL
CELLS
.
74
4.5.3.1
BIOPHYSICAL
PROPERTIES
OF
IF
IN
THE
EARLY
MURINE
ATRIAL
CELLS
.
74
4.5.3.2
EFFECT
OF
EXTRACELLULAR
POTASSIUM
ON
THE
REVERSAL
POTENTIAL
OF
IF
IN
THE
EARLY
MURINE
ATRIAL
CELLS
.
74
4.5.3.3
THE
EXTRACELLULAR
EFFECT
OF
CS
+
ON
IF
IN
THE
EARLY
MURINE
ATRIAL
CELLS
74
4.5.4
EFFECT
OF
ISO
ON
THE
IF
IN
THE
EARLY
MURINE
EMBRYONIC
CARDIOMYOCYTES
78
4.5.5
EFFECT
OF
8-BR-CAMP
ON
IF
IN
THE
MURINE
EMBRYONIC
CARDIOMYOCYTES
AT
THE
EARLY
DEVELOPMENTAL
STAGE
.
82
4.5.6
EFFECT
OF
CATALYTIC
SUBUNIT
OF
PKA
ON
THE
IF
IN
THE
MURINE
EMBRYONIC
CARDIOMYOCYTES
AT
THE
EARLY
DEVELOPMENTAL
STAGE
.
86
4.5.7
P-ADRENERGIC
MODULATION
OF
IF
IN
THE
MURINE
EMBRYONIC
CARDIOMYOCYTES
AT
THE
LATER
DEVELOPMENTAL
STAGE
.
90
5.
DISCUSSION
.
95
6.
SUMMARY
.
108
7.
ZUSSAMMENFASSUNG
.
ILL
8.
REFERENCES
.
113
9.PUBLICATIONS
.
131
10.
CURRICULUM
VITAE
.
133 |
| any_adam_object | 1 |
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| author_GND | (DE-588)124286895 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017074510 |
| illustrated | Illustrated |
| indexdate | 2024-08-17T00:41:58Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010300892 |
| oclc_num | 51713003 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | X, 133 S. graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| record_format | marc |
| spelling | LU, Zhongju 1972- Verfasser (DE-588)124286895 aut Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese von Zhongju Lu 2002 X, 133 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2002 Hochschulschrift gtt (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010300892&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | LU, Zhongju 1972- Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese Hochschulschrift gtt |
| subject_GND | (DE-588)4113937-9 |
| title | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese |
| title_auth | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese |
| title_exact_search | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese |
| title_full | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese von Zhongju Lu |
| title_fullStr | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese von Zhongju Lu |
| title_full_unstemmed | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese von Zhongju Lu |
| title_short | Etablierung der ionalen Mechanismen kardialer Erregung während der frühen Kardiomyogenese |
| title_sort | etablierung der ionalen mechanismen kardialer erregung wahrend der fruhen kardiomyogenese |
| topic | Hochschulschrift gtt |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010300892&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT luzhongju etablierungderionalenmechanismenkardialererregungwahrendderfruhenkardiomyogenese |