Protein kinase C protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, N.J.
Humana Press
2003
|
| Series: | Methods in molecular biology
233 |
| Subjects: | |
| Online Access: | Table of contents Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XVI, 582 S. Ill., graph. Darst. |
| ISBN: | 1588290689 |
Staff View
MARC
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| 245 | 1 | 0 | |a Protein kinase C protocols |c ed. by Alexandra C. Newton |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2003 | |
| 300 | |a XVI, 582 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 233 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Protéine kinase C - Manuels de laboratoire | |
| 650 | 4 | |a Protein Kinase C | |
| 650 | 4 | |a Protein kinase C |v Laboratory manuals | |
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Record in the Search Index
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| adam_text | Contents
Preface vii
Contributors xiii
Part I. Introduction
1 The Ins and Outs of Protein Kinase C
Alexandra C. Newton 3
2 Early Studies of Protein Kinase C: A Historical Perspective
Yasutomi Nishizuka and Ushio Kikkawa 9
Part II. Expression and Purification
3 Expression and Purification of Protein Kinase C
from Insect Cells
Hideyuki Mukai and Yoshitaka Ono 21
4 Expression and Purification of Protein Kinase C8 from Bacteria
Michael Gschwendt and Luise Stempka 35
Part III. Measuring the Activity of Protein Kinase C
5 Complexities in Protein Kinase C Activity Assays: An Introduction
Julianne J. Sando 45
6 Enzyme Assays for Protein Kinase C Activity
Julianne J. Sando and Jacquelyn K. Beals 63
7 Subcellular Translocation of Protein Kinase C
Julianne J. Sando, Jacquelyn K. Beals,
and Isa M. Hussaini 77
Part IV. Measuring the Interaction of Protein Kinase C with Membranes
8 Measuring the Interaction of Protein Kinase C with Membranes:
An Introduction
Alexandra C. Newton 89
ix
x Contents
9 Fluorescence Imaging of Protein Kinase C Translocation
in Living Cells
NaoakiSaito 93
10 Measuring the Binding of Protein Kinase C to
Sucrose-Loaded Vesicles
Jennifer Giorgione and Alexandra C. Newton 105
11 Use of Stopped-Flow Fluorescence Spectroscopy
to Measure Rapid Membrane Binding by Protein Kinase C
Eric A. Nalefski and Alexandra C. Newton 115
12 [3H]Phorbol 12,13-Dibutyrate Binding Assay for Protein
Kinase C and Related Proteins
Nancy E. Lewin and Peter M. Blumberg 129
Part V. Measuring the Phosphorylation of Protein Kinase C
13 Protein Kinase C Phosphorylation: An Introduction
Peter J. Parker 159
14 Pulse-Chase Analysis of Protein Kinase C
Mikiko Takahashi and Yoshitaka Ono 163
15 PDK-1 and Protein Kinase C Phosphorylation
AlexToker 171
16 In Vitro Autophosphorylation of Protein Kinase C Isozymes
Antonio M. Pepio and Wayne S. Sossin 191
17 Tyrosine Phosphorylation of Protein Kinase C
Toshiyoshi Yamamoto, Emiko Yamauchi, Hisaaki Taniguchi,
Hidenori Matsuzaki, and Ushio Kikkawa 207
18 Methods to Study Dephosphorylation of Protein
Kinase C In Vivo
Karen England and Martin Rumsby 217
19 Phosphopeptide-Specific Antibodies to Protein Kinase C
Wayne S. Sossin 233
Part VI. Identifying Protein Kinase C Substrates
20 Identifying Protein Kinase C Substrates: An Introduction
AlexToker 247
21 A Chemical Genetic Approach for the Identification of Direct
Substrates of Protein Kinases
Kavita Shah and Kevan M. Shokat 253
Contents xi
22 Studying the Optimal Peptide Substrate Motifs of Protein
Kinase C Using Oriented Peptide Libraries
Michael B. Yaffe 273
Part VII. Structural Analysis of Protein Kinase C
23 Structural Analysis of Protein Kinase C: An Introduction
James H. Hurley 289
24 Bacterial Expression and Purification of C1 and C2 Domains
of Protein Kinase C Isoforms
Wonhwa Cho, Michelle Digman, Bharath Ananthanarayanan,
and Robert V. Stahelin 291
25 Crystallization of the Protein Kinase C8 C1B Domain
Gongyi Zhang and James H. Hurley 299
Part VIII. Methods for Detecting Binding Proteins
26 Methods for Detecting Binding Proteins: An Introduction
Gerda Endemann and Daria Mochly-Rosen 307
27 Detection of Protein Kinase-Binding Partners by the Yeast
Two-Hybrid Analysis
Betty Y. Chang and Christine A. Cartwright 327
28 Glutathione S-Transferase Pull-Down Assay
Deborah Schechtman, Daria Mochly-Rosen, and Dorit Ron 345
29 Overlay Method for Detecting Protein-Protein Interactions
Deborah Schechtman, Christopher Murriel, Rachel Bright,
and Daria Mochly-Rosen 351
30 An Overlay Assay for Detecting Protein Kinase C-Binding Proteins
and Substrates
Susan Jaken 359
31 Functional Proteomic Analysis of the Protein Kinase C
Signaling System
Jason M. Pass, Jun Zhang, Thomas M. Vondriska,
and Peipei Ping 369
Part IX. Pharmacological Probes for Protein Kinase C
32 Pharmacological Probes for Protein Kinase C: An Introduction
MarceloG. Kazanietz 389
xii Contents
33 Applications of Inhibitors for Protein Kinase C and Their Isoforms
Garry X. Shen, Kerrie J. Way, Judith R. C. Jacobs,
and George L. King 397
34 Phorbol Esters as Probes for the Study of Protein
Kinase C Function
Marcelo G. Kazanietz and Patricia S. Lorenzo 423
35 Irreversible Inactivation of Protein Kinase C Isozymes by
Thiol-Reactive Peptide Substrate Analogs
Catherine A. O Brian, Nancy E. Ward, Feng Chu,
and Jubilee R. Stewart 441
Part X. Genetic Approaches to Studying Protein Kinase C
36 Genetic Approaches to Studying Protein Kinase C: An Introduction
Robert O. Messing 453
37 Animal Models in the Study of Protein Kinase C Isozymes
Doo-Sup Choi and Robert O. Messing 455
38 Genetic Manipulation of Protein Kinase C In Vivo
AlexToker 475
39 Yeast as a Host to Screen for Modulators and Regulatory Regions
of Mammalian Protein Kinase C Isoforms
Amadeo M. Parissenti and Heimo Riedel 491
Part XI. Protein Kinase C in Disease
40 Protein Kinase C in Disease: Cancer
Alan P. Fields and W. Clay Gustafson 519
41 Characterization of the Role of Protein Kinase C Isozymes in
Colon Carcinogenesis Using Transgenic Mouse Models
Alan P. Fields, Nicole R. Murray, and W. Clay Gustafson 539
42 Alcohol Addiction
M. Foster Olive and Dorit Ron 555
Index 571
|
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| indexdate | 2024-07-09T19:12:31Z |
| institution | BVB |
| isbn | 1588290689 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protein kinase C protocols ed. by Alexandra C. Newton Totowa, N.J. Humana Press 2003 XVI, 582 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 233 Includes bibliographical references and index Protéine kinase C - Manuels de laboratoire Protein Kinase C Protein kinase C Laboratory manuals Signal Transduction Proteinkinase C (DE-588)4306407-3 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteinkinase C (DE-588)4306407-3 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Newton, Alexandra C. Sonstige oth Methods in molecular biology 233 (DE-604)BV035362695 233 http://www.loc.gov/catdir/toc/fy038/2003042346.html Table of contents HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010257943&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein kinase C protocols Methods in molecular biology Protéine kinase C - Manuels de laboratoire Protein Kinase C Protein kinase C Laboratory manuals Signal Transduction Proteinkinase C (DE-588)4306407-3 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)4306407-3 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Protein kinase C protocols |
| title_auth | Protein kinase C protocols |
| title_exact_search | Protein kinase C protocols |
| title_full | Protein kinase C protocols ed. by Alexandra C. Newton |
| title_fullStr | Protein kinase C protocols ed. by Alexandra C. Newton |
| title_full_unstemmed | Protein kinase C protocols ed. by Alexandra C. Newton |
| title_short | Protein kinase C protocols |
| title_sort | protein kinase c protocols |
| topic | Protéine kinase C - Manuels de laboratoire Protein Kinase C Protein kinase C Laboratory manuals Signal Transduction Proteinkinase C (DE-588)4306407-3 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Protéine kinase C - Manuels de laboratoire Protein Kinase C Protein kinase C Laboratory manuals Signal Transduction Proteinkinase C Untersuchungsmethode Aufsatzsammlung |
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