G protein signaling: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2003
|
| Series: | Methods in molecular biology
237 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XII, 249 S. Ill. |
| ISBN: | 1588291375 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Preface v
Contributors xi
Part I. Purification of G Protein Subunits and G Protein Effectors
1 Purification of Recombinant G Protein a Subunits
from Escherichia coli
Wendy K. Greentree and Maurine E. Under 3
2 Purification of G Protein Subunits from Sf9 Insect Cells Using
Hexahistidine Tagged a and Py Subunits
Tohru Kozasa 21
3 Expression and Purification of Soluble Adenylyl Cyclase from
Eschericia coli
Jeff A. Beelerand Wei Jen Tang 39
4 Purification of Phospholipase C p and Phospholipase C e
from Sf9 Cells
Mousumi Ghosh, Huan Wang, Grant G. Kelley,
and Alan V. Smrcka 55
Part II. Assays for G Protein Dependent Effector Activation
In Vitro
5 Assay for G Protein Dependent Activation of Phospholipase C p
Using Purified Protein Components
Mousumi Ghosh and Alan V. Smrcka 67
6 Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells
Ronald Taussig 77
Part III. Assays for G Protein Coupled Receptor Mediated
and G Protein Subunit Mediated Effector Activation in Intact
Cells
7 Measurement of G Protein Coupled Receptor Stimulated
Phospholipase D Activity in Intact Cells
Stephanie J. Walker and H. Alex Brown 89
8 Analysis of G Protein Mediated Activation of Phospholipase C
in Cultured Cells
Bo Liu and Dianqing Wu 99
vii
viii Contents
Part IV. Receptor Mechanisms, Activity, and Localization
9 Intensive Mutational Analysis of G Protein Coupled Receptors
in Yeast
Andjelka Celic, Sara M. Connelly, Negin P. Martin,
and Mark E. Dumont 105
10 Green Fluorescent Protein Tagged p Arrestin Translocation
as a Measure of G Protein Coupled Receptor Activation
Stephens. G. Ferguson and Marc G. Caron 121
11 Detection of G Protein Coupled Receptors by Immunofluorescence
Microscopy
Patricia M. Hinkle and John A. Puskas 127
12 Assay of G Protein Coupled Receptor Activation of G Proteins
in Native Cell Membranes Using [35S]GTPyS Binding
Jean M. Bidlack and Amy L. Parkhill 135
13 Analysis of the Coupling of G12/13 to G Protein Coupled Receptors
Using a Luciferase Reporter Assay
Bo Liu and Dianqing Wu 145
Part V. Disruption of Endogenous G Protein Signaling Systems
to Understand the Role of G Protein Subunits in Intact Systems
14 Selective Inhibition of G Protein Mediated Pathways Using
RGS Domains
Tohru Kozasa and Richard D. Ye 153
15 Ribozymes as Tools for Suppression of G Protein y Subunits
Janet D. Robishaw, Zheng ping Guo, and Qin Wang 169
16 In Vivo Adenoviral Mediated Gene Transfer of the pARKct
to Study the Role of Gpy in Arterial Restenosis
Guido laccarino and Walter J. Koch 181
Part VI. Posttranslational Modifications of Regulators of G
Protein Signaling
17 Analysis of RGS Protein Palmitoylation
Leah S. Bernstein, Maurine E. Under, and John R. Hepler 195
18 Methods for Measuring RGS Protein Phosphorylation by G
Protein Regulated Kinases
Susanne Hollinger and John R. Hepler 205
ix Contents
Part VII. Use of GFP Tagged G Proteins and Effectors
in Intact Cells
19 The Use of Green Fluorescent Proteins to View Association
Between Phospholipase Cp and G Protein Subunits in Cells
Suzanne Scarlata and Louisa Dowal 223
20 Cellular Localization of GFP Tagged a Subunits
Thomas R. Hynes, Thomas E. Hughes,
and Catherine H. Berlot 233
Index 247
|
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| indexdate | 2024-07-09T19:12:31Z |
| institution | BVB |
| isbn | 1588291375 |
| language | English |
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| physical | XII, 249 S. Ill. |
| publishDate | 2003 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | G protein signaling methods and protocols ed. by Alan V. Smrcka Totowa, NJ Humana Press 2003 XII, 249 S. Ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 237 Includes bibliographical references and index GTP-bindende Proteine (DE-588)4248354-2 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content GTP-bindende Proteine (DE-588)4248354-2 s Signaltransduktion (DE-588)4318717-1 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Smrcka, Alan V. Sonstige oth Methods in molecular biology 237 (DE-604)BV035362695 237 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010257941&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | G protein signaling methods and protocols Methods in molecular biology GTP-bindende Proteine (DE-588)4248354-2 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4248354-2 (DE-588)4324907-3 (DE-588)4318717-1 (DE-588)4143413-4 |
| title | G protein signaling methods and protocols |
| title_auth | G protein signaling methods and protocols |
| title_exact_search | G protein signaling methods and protocols |
| title_full | G protein signaling methods and protocols ed. by Alan V. Smrcka |
| title_fullStr | G protein signaling methods and protocols ed. by Alan V. Smrcka |
| title_full_unstemmed | G protein signaling methods and protocols ed. by Alan V. Smrcka |
| title_short | G protein signaling |
| title_sort | g protein signaling methods and protocols |
| title_sub | methods and protocols |
| topic | GTP-bindende Proteine (DE-588)4248354-2 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | GTP-bindende Proteine Untersuchungsmethode Signaltransduktion Aufsatzsammlung |
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