Fundamental bacterial genetics:
"Fundamental Bacterial Genetics presents a concise introduction to microbial genetics. The text focuses on one bacterial species, Escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. A solid balance of...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Malden, MA [u.a.]
Blackwell Science
2004
|
| Ausgabe: | 1. publ. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | "Fundamental Bacterial Genetics presents a concise introduction to microbial genetics. The text focuses on one bacterial species, Escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. A solid balance of concepts, techniques, and applications makes this book an accessible, essential introduction to the theory and practice of microbial genetics."--BOOK JACKET. |
| Beschreibung: | XIII, 287 S. Ill., graph. Darst. |
| ISBN: | 0632044489 |
Internformat
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| 100 | 1 | |a Trun, Nancy Jo |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fundamental bacterial genetics |c Nancy Trun and Janine Trempy |
| 250 | |a 1. publ. | ||
| 264 | 1 | |a Malden, MA [u.a.] |b Blackwell Science |c 2004 | |
| 300 | |a XIII, 287 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 520 | 1 | |a "Fundamental Bacterial Genetics presents a concise introduction to microbial genetics. The text focuses on one bacterial species, Escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. A solid balance of concepts, techniques, and applications makes this book an accessible, essential introduction to the theory and practice of microbial genetics."--BOOK JACKET. | |
| 650 | 4 | |a Escherichia coli - Génétique | |
| 650 | 4 | |a Génétique bactérienne | |
| 650 | 4 | |a Bacteria |x genetics | |
| 650 | 4 | |a Bacterial genetics | |
| 650 | 4 | |a DNA, Bacterial | |
| 650 | 4 | |a Escherichia coli |x Genetics | |
| 650 | 4 | |a Escherichia coli |x genetics | |
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Datensatz im Suchindex
| _version_ | 1804129803147673600 |
|---|---|
| adam_text | Brief con tents
V „ _ _ ^ _ ..._,..¦„¦. J
Full contents vi
Preface xii
Acknowledgments xiv
Chapter 1 Introduction to the cell 1
Chapter 2 The bacterial DNA molecule 17
Chapter 3 Mutations 38
Chapter 4 DNA repair 58
Chapter 5 Recombination 74
Chapter 6 Transposition 89
Chapter 7 Bacteriophage 105
Chapter 8 Transduction 126
Chapter 9 Natural plasmids 142
Chapter 10 Conjugation 156
Chapter 11 Transformation 175
Chapter 12 Gene expression and regulation 191
Chapter 13 Plasmids, bacteriophage, and transposons as tools 213
Chapter 14 DNA cloning 234
Chapter 15 Bioinformatics and proteomics 256
Glossary 267
Further reading 278
Index 281
^ — ^
Full contents I
Preface xii
Acknowledgments xxv
Chapter 1 Introduction to the cell 1
The bacterial cell: a quick overview 5
How do cells grow? * *
What is genetics? 15
Summary *6
Chapter 2 The bacterial DNA molecule 17
The structure of DNA and RNA 17
Deoxyribonucleosides and deoxyribonucleotides 17
DNA is only polymerized 5 to 3
Double stranded DNA 21
Supercoiling double stranded DNA ^
Replication of the Escherichia coli chromosome 26
Constraints that influence DNA replication ^
The replication machinery ^
DNApolymerases
DnaG primase
DnaA, DnaB, and DnaC 31
Replication of both strands
Theta mode replication 3
Minimizing mistakes in DNA replication
The DNA replication machinery as molecular tools
Summary ¦*
Chapter 3 Mutations *S
Phenotype and genotype
Classes of mutations
Point mutations and their consequences
Measuring mutations: rate and frequency
Spontaneous and induced mutations
Errors during DNA replication: incorporation errors ,.
Errors due to tautomerism
( Full Contents ) vl
Spontaneous alteration by depurination 45
Spontaneous alteration by deamination 46
Alterations by spontaneous genetic rearrangement 48
Alterations caused by transposition 48
Induced mutations 48
Chemicals that mimic normal DNA bases: base analogs 49
Chemicals that react with DNA bases: base modifiers 49
Chemicals that bind DNA bases: intercalators 51
Mutagens that physically damage the DNA: ultraviolet light and ionizing
radiation 51
Mutator strains 52
Reverting mutations 52
Suppression 53
Ames test 54
How have we exploited bacterial mutants? 5 6
Summary 56
Chapter 4 DNA repair 58
Lesions that constitute DNA damage 58
Reverse, excise or tolerate? 60
Mechanisms that reverse DNA damage 60
Photoreactivation 60
O6 methylguanine or O4 methylthymine methyltransferase 61
Mechanisms that excise DNA damage 62
UvrABC directed nucleotide excision repair 62
MutHLS methyl directed mismatch repair 65
Very short patch repair 65
Glycosylases 67
Uracil N glycosylase coupled with AP excision repair 67
Deaminated bases removed by DNA glycosylase 68
Alkylated bases removed by DNA glycosylase 68
MutM/MutY: oxidative damage 68
N glycosylases specific for pyrimidine dimers 68
Mechanisms that tolerate DNA damage 69
Transdimer synthesis 69
Post replication/recombinational repair (PRR) 69
Introduction to the SOS regulon 71
Summary 72
Chapter 5 Recombination 74
Homologous recombination 74
Models for homologous recombination 78
The Holliday or double strand invasion model of recombination 79
An alternative to the Holliday model: the single strand invasion model
of Meselson and Radding 81
Further enzymatic considerations 82
Site specific recombination 83
A typical site specific recombinational event 83
Bacteriophage X: a model for site specific recombination 84
Other microbial examples of site specific recombination 85
(_ Full Contents^)
Illegitimate recombination 86
Summary 87
Chapter 6 Transposition 89
The structure of transposons 89
The frequency of transposition 91
The two types of transposition reactions 93
The transposition machinery 93
Accessory proteins encoded by the transposon 94
Accessory proteins encoded by the host 94
Non replicative transposition 95
Replicative transposition 95
Does the formation of a cointegrate predict the transposition mechanism? 97
The fate of the donor site 97
Target immunity 99
Transposons as molecular tools 100
Summary 104
Chapter 7 Bacteriophage 105
The structure of phage 105
The lifecycle of a bacteriophage 106
Lytic lysogenic options 107
The X lifecycle 1°7
X adsorption 107
X DNA injection 1°7
Protecting the X genome in the bacterial cytoplasm 109
What happens to the X genome after it is stabilized? 109
X and the lytic lysogenic decision HO
The X lysogenic pathway 1 ^
The X lytic pathway ll3
DNA replication during the X lytic pathway 114
Making X phage 1 ^
Getting out of the cell the X S and R proteins 1l6
Induction of A, by the SOS system 117
Superinfection H
Restriction and modification of DNA 1*
The lifecycle of M13 ll8
M13 adsorption and injection l
Protection of the Ml 3 genome ll8
M13 DNA replication ll8
M13 phage production and release from the cell *
The lifecycle of PI ll9
Adsorption, injection, and protection of the genome *
PI DNA replication and phage assembly
The location of the P1 prophage in a lysogen *
PI transducing particles *
The lifecycle of T4 l21
T4 adsorption and injection
T4rII mutations and the nature of the genetic code
Summary l2
( Full Contents ) I
Chapter 8 Transduction 126
Generalised transduction vs. specialized transduction 126
PI as a model for generalized transducing phage 126
Packaging the chromosome 127
Moving pieces of the chromosome from one cell to another 128
Identifying transduced bacteria: selection vs. screening 129
Carrying out a transduction 129
Uses for transduction 130
Two factor crosses to determine gene linkage 131
Mapping the order of genes three factor crosses 132
Strain construction 133
Localized mutagenesis 133
Specialized transducing phage 134
Making merodiploids with specialized transducing phage 135
Moving mutations from plasmids to specialized transducing phage to
the chromosome 138
Summary 140
Chapter 9 Natural plasmids 142
Origins of replication 142
Plasmid copy number 145
Setting the copy number 148
Plasmid incompatibility 148
Plasmid amplification 149
Other genes that can be carried by plasmids 149
Plasmids can be circular or linear DNA 152
Broad host range plasmids 152
Moving plasmids from cell to cell 153
Summary 154
Chapter 10 Conjugation 156
The F factor 156
The R factors 156
The conjugation machinery 158
Transfer of the DNA 158
Surface exclusion 159
F,Hfr,orF prime 160
Formation of the Hf r 161
Transfer of DNA from an Hfr to another cell 162
Formation of F primes 163
Transfer of F primes from one cell to another 164
Genetic uses of F primes 165
Genetic uses of Hfr strains—mapping genes on the E. coli chromosome
using Hfr crosses 167
The 50% rule 171
Using several Hfr strains to cover the chromosome 171
Mobilization of non conjugatible plasmids by R and F 172
Conjugation from prokaryotes to eukaryotes 173
Summary 173
f~FuH Contents *)
Chapter 11 Transformation 175
Natural competency 176
The process of natural transformation 183
The machinery of naturally transformable cells 184
Artificial transformation 187
Transformation as a genetic tool: gene mapping 188
Transformation as a molecular tool 188
Summary 189
Chapter 12 Gene expression and regulation 191
The players in the regulation game 192
Operons and regulons 196
Repression of the lac operon 197
Activation of the lac operon by cyclic AMP and the CAP protein 200
Regulation of the tryptophan biosynthesis operon by attenuation 200
Regulation of the heat shock regulon by an alternate sigma factor, mRNA
stability, and proteolysis 205
Regulation of the SOS regulon by proteolytic cleavage of the repressor 208
Two component regulatory systems: signal transduction and the cps regulon 209
Summary 211
Chapter 13 Plasmids, bacteriophage,
and transposons as tools 213
What is a cloning vector? 213
Why not use naturally occurring plasmids as vectors? 215
The importance of copy number 215
An example of how a cloning vector works—pBR322 215
Multiple cloning sites 216
Determining which plasmids contain an insert 217
Expression vectors 217
Vectors for purifying the cloned gene product 219
Vectors for localizing the gene product 221
Vectors for studying gene expression 221
Shuttle vectors 224
Artificial chromosomes 225
Constructing phage vectors 225
Suicide vectors 227
Phage display vectors 228
Combining phage vectors and transposons 2
Summary 232
Chapter 14 DNA cloning 234
Isolating DNA from cells 23
Plasmid DNA isolation 23
Chromosomal DNA isolation 2
Cutting DNA molecules 23
Type I restriction modification systems ,
Type II restriction modification systems l
Type III restriction modification systems
( Full Contents ) ;
Restriction modification as a molecular tool 23 7
Generate double stranded breaks in DNA by shearing the DNA 239
Joining DNA molecules 239
Manipulating the ends of molecules 240
Visualizing the cloning process 241
Constructing libraries of clones 242
DNA detection—Southern blotting 243
DNA amplification—polymerase chain reaction 245
Adding novel DNA sequences to the ends of a PCR amplified sequence 248
Site directed mutagenesis using PCR 248
Cloning and expressing a gene 249
DNA sequencing using dideoxy sequencing 251
DNA sequence searches 253
Summary 254
Chapter 15 Bioinformatics and proteomics 256
Bioinformatics 256
Strategies for sequencing genomes 257
Bacterial genomes 259
Analyzing genomes 260
The E. coli K12 genome 261
Proteomics 262
Techniques for examining the proteome—SDS PAGE and 2 D PAGE 262
Techniques for examining the proteome—microarray technology 264
Summary 266
Glossary 267
Further reading 278
Index 281
|
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| discipline | Biologie |
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| id | DE-604.BV016474895 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:10:56Z |
| institution | BVB |
| isbn | 0632044489 |
| language | English |
| lccn | 2003000141 |
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| physical | XIII, 287 S. Ill., graph. Darst. |
| publishDate | 2004 |
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| publisher | Blackwell Science |
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| spelling | Trun, Nancy Jo Verfasser aut Fundamental bacterial genetics Nancy Trun and Janine Trempy 1. publ. Malden, MA [u.a.] Blackwell Science 2004 XIII, 287 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier "Fundamental Bacterial Genetics presents a concise introduction to microbial genetics. The text focuses on one bacterial species, Escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. A solid balance of concepts, techniques, and applications makes this book an accessible, essential introduction to the theory and practice of microbial genetics."--BOOK JACKET. Escherichia coli - Génétique Génétique bactérienne Bacteria genetics Bacterial genetics DNA, Bacterial Escherichia coli Genetics Escherichia coli genetics Bakterien (DE-588)4004296-0 gnd rswk-swf Genetik (DE-588)4071711-2 gnd rswk-swf Bakterien (DE-588)4004296-0 s Genetik (DE-588)4071711-2 s DE-604 Trempy, Janine E. Verfasser aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010185548&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Trun, Nancy Jo Trempy, Janine E. Fundamental bacterial genetics Escherichia coli - Génétique Génétique bactérienne Bacteria genetics Bacterial genetics DNA, Bacterial Escherichia coli Genetics Escherichia coli genetics Bakterien (DE-588)4004296-0 gnd Genetik (DE-588)4071711-2 gnd |
| subject_GND | (DE-588)4004296-0 (DE-588)4071711-2 |
| title | Fundamental bacterial genetics |
| title_auth | Fundamental bacterial genetics |
| title_exact_search | Fundamental bacterial genetics |
| title_full | Fundamental bacterial genetics Nancy Trun and Janine Trempy |
| title_fullStr | Fundamental bacterial genetics Nancy Trun and Janine Trempy |
| title_full_unstemmed | Fundamental bacterial genetics Nancy Trun and Janine Trempy |
| title_short | Fundamental bacterial genetics |
| title_sort | fundamental bacterial genetics |
| topic | Escherichia coli - Génétique Génétique bactérienne Bacteria genetics Bacterial genetics DNA, Bacterial Escherichia coli Genetics Escherichia coli genetics Bakterien (DE-588)4004296-0 gnd Genetik (DE-588)4071711-2 gnd |
| topic_facet | Escherichia coli - Génétique Génétique bactérienne Bacteria genetics Bacterial genetics DNA, Bacterial Escherichia coli Genetics Escherichia coli genetics Bakterien Genetik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010185548&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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