Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Stuttgart
Selbstverl., Maierstr.9A, 70567 Stuttgart
2002
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Tübingen, Univ., Diss., 2002 |
| Physical Description: | VI, 91 S.: Ill., graph. Darst. 21 cm |
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MARC
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| 100 | 1 | |a Fricke, Cornelia |d 1972- |e Verfasser |0 (DE-588)124395627 |4 aut | |
| 245 | 1 | 0 | |a Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio |c von Cornelia Fricke |
| 264 | 1 | |a Stuttgart |b Selbstverl., Maierstr.9A, 70567 Stuttgart |c 2002 | |
| 300 | |a VI, 91 S.: Ill., graph. Darst. |b 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Tübingen, Univ., Diss., 2002 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010159308 | |
Record in the Search Index
| _version_ | 1808226281818423296 |
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| adam_text |
TABLE
OF
CONTENTS
1
INTRODUCTION
.
1
1.1
THE
ZEBRAFISH
AS
A
MODEL
SYSTEM
FOR
RGC
AXON
PATHFINDING
1
12
DEVELOPMENT
OF
THE RETINAL
PROJECTION
IN
ZEBRAFISH
13
ROUNDABOUT
(ROBO)
AND
THE
GUIDANCE
OF
AXONS
AT
THE
MIDLINE
_
_
_4
14
DCC
AND
ROBO
CAN
FORM
A
RECEPTOR
COMPLEX
AND
ACT
TOGETHER
IN
AXON
GUIDANCE
AT
THE
MIDLINE
15
ANALYSIS
OF
RGC
AXON
GUIDANCE
2
MATERIALS
AND
METHODS
.
9
2.1
FREQUENTLY
USED
22
FISH WORK
-
A
-
-
-
-
W
-
YY
12
23
KITS
_
12
24
PRIMERS
13
25
PAC
CLONES
_
-
_
_
_
_
_
13
16
D^J^^
O
-
O
-
-
ON
-
-
A
-
-
-
-
-
-
-
-
-
-
-
-
A
-
-
14
2.6.1
DNA
PREPARATION
FROM
SINGLE
ZEBRAFISH
LARVAE
FOR
POLYMERASE
CHAIN
REACTION
(PCR)
.
14
2.62
DNA
PREPARATION
FROM
FIN-CLIPS
FOR
PCR
.14
2.6.3
BOILING
MINIPREP
FOR
PLASMID
DNA
ISOLATION
.
15
2.6.4
ISOLATION
OF
PL-ARTIFICIAL
CHROMOSOME
(PAC)
DNA
.
15
2.7
2.7.1
SEQUENCE
ANALYSIS
.
16
23
GENETIC
AND
RH
MAPPING
_
_
-
_
_
_16
25
METHOD
FOR
ALLELE
SEQUENCING
-
-
O
-
-
-
-
-
-
O
-
-
-
16
2.10
METHOD
FOR
SEQUENCING
OF
FOUNDER
AND
CONTROL
DNA
_
_
_
17
2.11
RESTRICTION
FRAGMENT
LENGTH
POLYMORPHISM
(RFLP)
.
_
_
18
TABLE
OF
CONTENTS
2.12
ALLELE
SPECIFIC
AMPLIFICATION
(ASA)
.
18
2.13
LIBRARY
SCREENS
_
19
2.14
DEGENERATE
PCR
20
2.15
5
'
AND
3
'
RAPID
AMPLIFICATION
OF
CDNA
ENDS
(RACE).
-
-
.
-
.YY.YY.YY.YY.YY.YY.YY._.YY._.YY.YY._.20
2.16
RT-PCR
TO
SPAN
THE
MYSTERIOUS REGION
IN
CLONE
DCC16D-31.-.-.
-
.
21
2.17
GENERATION
OF
CONSTRUCTS
EXPRESSING
ASUROBOL
IN
RGCS._
.
21
2.18
ANALYSIS
OF
RETINOTECTAL PROJECTION
BY
LIPOPHILIC
DYE
INJECTIONS
.
23
2.19
WHOLE
MOUNT
STAINING
AND
SECTIONING.
-
--------
23
2.19.1
IN
SITU
HYBRIDIZATION
.
23
2.19.2
ASRNA
PROBES
FOR
IN
SITU
HYBRIDIZATION
.
24
2.193
ANTIBODY
STAINING
.
25
2.19.4
HISTOLOGICAL
ANALYSIS
.
25
3
RESULTS
.
26
3.1
CLOIULLG
OF
ASTRAY
^^0
MI
^6
3.1.1
SELECTION
OF
AST
.
26
3.1.2
MAPPING
OF
AST
.
26
3.1.3
SELECTION
OF
ROBO2
AS
A
CANDIDATE
GENE
FOR
AST
.
28
3.1.4
SPATIOTEMPORAL
ANALYSIS
OF
ROBO2
EXPRESSION IN
THE
EYE
AND
ALONG
THE
PATH
OF
RGC
AXONS
.29
3.1
S
MAPPING
OF
ROBO2
REVEALS
LINKAGE
BETWEEN
AST
AND
ROBO2
.
30
3.1.6
ANALYSIS
OF
RESTRICTION
FRAGMENT
LENGTH
POLYMORPHISMS
(RFLPS)
IN
ROBO2
CONFIRMS
LINKAGE
BETWEEN
AST
AND
ROBO2
.
31
3.1.7
SEQUENCING
OF
ROBO2
CODING
REGION
IN
TWO
MUTANT
AST
ALLELES
.
34
3.13
LINKAGE
ANALYSIS
BY
ALLELE
SPECIFIC
AMPLIFICATION
(ASA)
.37
3.1.9
AST*
376
AND
AST
031
MAP
ROUGHLY
TO
DIE
SAME
PLACE
AS
ROBO2
.39
3.1.10
ASTLROBO2
MRNA
EXPRESSION
IS
REDUCED
IN
ASF
272
*
.
40
N
TABLE
OF
CONTENTS
3.1.11
ALTERNATIVE
SPLICE
FORMS
OF
ASTLROBO2
.
40
3.1.12
GENERATION
OF
CONSTRUCTS
EXPRESSING
ASTIROBO2
UNDER
THE
CONTROL
OF
RGC-SPECIFIC
PROMOTERS
.40
3.1.13
ISOLATION
OF
P-1
DERIVED
ARTIFICIAL
CHROMOSOME
(PAC)
CLONES
COVERING
AST/ROBO2
.
42
3.1.13.1
TESTING
OF
PAC
ENDS
ON
THE
C7
DEFICIENCY
.
42
3.1.132
ASSEMBLY
OF
A
PAC
CONTIG
.
44
3.1.13.3
BEGINNING
STUDY
OF
THE
GENOMIC
STRUCTURE
OF
ROBO2
.
46
32
CHARACTERIZATION
OF
THE
ASTLROBO2
MUTANT
PHENOTYPE
32.1
RGC
AXONS
AT
THE
OPTIC
DISC
IN
ASTLROBO2
.
47
322
OPTIC
CHIASM
MALFORMATION
IN
AST!ROBO2
.
49
323
ZNP-1
STAINING
REVEALS
PATHFINDING
MISTAKES
OF
THE
OPTIC
NERVE
IN
ASTLROBO2
.
52
32.4
ASTLROBO2
IS
REQUIRED
FOR
OPTIC
TRACT
FORMATION
.
54
325
ANALYSIS
OF
THE
MORPHOLOGY
OF
THE
RETINA
IN
ASTLROBO2
.
55
32.6
SURVIVAL
OF
ASTHROBO2
HOMOZYGOUS
MUTANT
INDIVIDUALS
TO
ADULTHOOD
.
59
33
CLONING
AND
EXPRESSION
ANALYSIS
OF
A
DELETED
IN
COLORECTAL
CANCER
(DEC)
GENE
IN
ZEBRAFISH
~^
-
60
33.1
CLONING
OF
A
ZEBRAFISH
DEC
GENE
.
60
332
EXPRESSION
PATTERN
OF
DEC
IN
EMBRYONIC
AND
LARVAL
ZEBRAFISH
.
63
4
DISCUSSION
.
70
*.1
CLONING
AND
CHARACTERIZATION
OF
AST!ROBO2
MAOAOEOOAOOAWAOAEOAOAMOEOOEMMEOAWMMMAMMAAAEOMMAOWMMMAOAOMAOWOO*********
70
4.1.1
AST
ENCODES
ZEBRAFISH
ROBO2
.
70
4.1
2
INTERPRETATION
OF
EYE
TRANSPLANTATION
RESULTS
IN
LIGHT
OF
THE
EXPRESSION
PATTERN
OF
AST/ROBO2
AND
THE
MOLECULAR
NATURE
OF
THE
ASF
2721
ALLELE
.
71
4.13
ALTERNATIVE
SPLICING
IN
AST!ROBO2
.
72
4.1.4
ANALYSIS
OF
THE
ASTLROBO2
MUTANT
PHENOTYPE
.
74
4.15
LOCAL
RGC
AXON
PATHFINDING
AT
THE
OPTIC
DISC
APPEARS
NORMAL
IN
ASTIROBO2
.74
4.1.6
MALFORMATION
OF
THE
OPTIC
CHIASM
IN
ASTLROBO2
.
75
III
TABLE
OF
CONTENTS
4.1.7
MALFORMATION
OF
THE
OPTIC
TRACT
IN
ASTLROBO2
.
77
4
J
CLONING
AND
EXPRESSION
OF
DEC
IN
5
APPENDICES
1-5
.
81
6
REFERENCES
.
84
7
CURRICULUM
VITAE
.
91
IV |
| any_adam_object | 1 |
| author | Fricke, Cornelia 1972- |
| author_GND | (DE-588)124395627 |
| author_facet | Fricke, Cornelia 1972- |
| author_role | aut |
| author_sort | Fricke, Cornelia 1972- |
| author_variant | c f cf |
| building | Verbundindex |
| bvnumber | BV016429364 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV016429364 |
| illustrated | Not Illustrated |
| indexdate | 2024-08-24T00:22:42Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010159308 |
| oclc_num | 643998109 |
| open_access_boolean | |
| owner | DE-29T DE-12 |
| owner_facet | DE-29T DE-12 |
| physical | VI, 91 S.: Ill., graph. Darst. 21 cm |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Selbstverl., Maierstr.9A, 70567 Stuttgart |
| record_format | marc |
| spelling | Fricke, Cornelia 1972- Verfasser (DE-588)124395627 aut Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio von Cornelia Fricke Stuttgart Selbstverl., Maierstr.9A, 70567 Stuttgart 2002 VI, 91 S.: Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2002 Sehnerv (DE-588)4205981-1 gnd rswk-swf Morphogenese (DE-588)4132460-2 gnd rswk-swf Zebrabärbling (DE-588)4079468-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zebrabärbling (DE-588)4079468-4 s Sehnerv (DE-588)4205981-1 s Morphogenese (DE-588)4132460-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010159308&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fricke, Cornelia 1972- Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio Sehnerv (DE-588)4205981-1 gnd Morphogenese (DE-588)4132460-2 gnd Zebrabärbling (DE-588)4079468-4 gnd |
| subject_GND | (DE-588)4205981-1 (DE-588)4132460-2 (DE-588)4079468-4 (DE-588)4113937-9 |
| title | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio |
| title_auth | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio |
| title_exact_search | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio |
| title_full | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio von Cornelia Fricke |
| title_fullStr | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio von Cornelia Fricke |
| title_full_unstemmed | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio von Cornelia Fricke |
| title_short | Genetic analysis of retinal axon pathfinding in zebrafish, Danio rerio |
| title_sort | genetic analysis of retinal axon pathfinding in zebrafish danio rerio |
| topic | Sehnerv (DE-588)4205981-1 gnd Morphogenese (DE-588)4132460-2 gnd Zebrabärbling (DE-588)4079468-4 gnd |
| topic_facet | Sehnerv Morphogenese Zebrabärbling Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010159308&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT frickecornelia geneticanalysisofretinalaxonpathfindinginzebrafishdaniorerio |