Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | German |
| Published: |
2002
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Würzburg, Univ., Diss., 2002 |
| Physical Description: | III, 84 S. Ill., graph. Darst. |
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Record in the Search Index
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| adam_text |
CONTENT I
1* INTRODUCTION. L
1.1. HIGHER PLANT NITROGEN METABOLISM.1
1.2. NITRATE REDUCTASE.3
1.2.1. STRUCTURAL AND FUNCTIONAL CHARACTERISTICS.3
1.2.2. REGULATION.4
1.3. OXYGEN DEPRIVATION - CAUSE, EFFECT AND ADAPTATION
MECHANISMS.7
1.4. NITRATE, NITRATE REDUCTASE AND ANOXIA.10
1.5. THE AIMS OF THIS STUDY:.13
2. MATERIAL AND METHODS.14
2.1. PLANT MATERIAL AND GROWTH CONDITIONS.14
2.2. FEEDING EXPERIMENTS.15
2.2.1. TUNGSTATE FEEDING.15
2.2.2. SUCROSE FEEDING.15
2.3. IN VITRO ASSAYS OF ENZYME ACTIVITIES.16
2.3.1. IN VITRO ASSAY OF NITRATE REDUCTASE ACTIVITY AND ACTIVATION
STATE.16
2.3.2. IN VITRO ASSAY OF LACTATE DEHYDROGENASE AND ALCOHOL
DEHYDROGENASE ACTIVITIES.16
2.4. SOLUTE ANALYSIS.17
2.4.1. ANION DETERMINATION.17
2.4.2. NITRITE DETERMINATION.IS
2.4.3. CATION DETERMINATION.18
2.5. METABOLITE MEASUREMENTS 19
2.5.1. SUGARS.19
2.5.2. SUGAR PHOSPHATES. 19
2.5.3. STARCH ASSAY.20
2.5.4. ADENINE NUCLEOTIDES.20
2.5.5. L-LACTATE.21
2.5.6. ETHANOL. 22
2.5.7. AMINO ACIDS.22
2.6. TOTAL PROTEIN.23
2.7. OXYGEN CONSUMPTION OF ROOTS 23
2.8. ROOT MORPHOLOGY.23
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/967168015
CONTENT II
2.9.
3IP NMR SPECTROSCOPY.24
2.10. NO MEASUREMENTS.25
2.11. POROMETRY.26
2.12. LIST OF CHEMICALS.27
3. RESULTS.30
3.1. CHARACTERIZATION OF NORMOXIC METABOLISM OF LNR-H TOBACCO
PLANTS IN COMPARISON TO WT
.30
3.1.1. ROOT MORPHOLOGY AND ROOTISHOOT RATIO.30
3.1.2. POROMETRY.33
3.1.3. ANION AND CATION CONTENT.33
3.1.4. RESPIRATION.34
3.1.5. NUCLEOTIDE LEVELS.35
3.1.6. METABOLITE CONTENT IN WT AND LNR-H ROOTS.36
3.1.7. AMINO ACIDS AND TOTAL PROTEIN CONTENT.36
3.2.
ANOXIC METABOLISM OF LNR-H AND WT TOBACCO PLANTS.38
3.2.1. WHOLE PLANT RESPONSES TO SHORT TERM ANOXIA IN THE ROOT SYSTEM.38
3.2.2. NITRATE REDUCTASE ACTIVITY AND NITRITE PRODUCTION IN VIVO.39
3.2.3. ETHANOL PRODUCTION.41
3.2.4. LACTATE PRODUCTION.42
3.2.5. ALCOHOL DEHYDROGENASE ACTIVITY.44
3.2.6. LACTATE DEHYDROGENASE ACTIVITY.44
3.2.7. NITRATE REDUCTASE, ETHANOL AND LACTATE PRODUCTION IN TUNGSTATE ,
TREATED WT PLANTS.45
3.2.8. FREE SUGARS AND HEXOSE MONOPHOSPHATES.47
3.2.9. SUCROSE FEEDING EXPERIMENTS.51
3.2.10. ADENINE NUCLEOTIDES.53
3.2.11.
3,P NMR MEASUREMENTS OF CYTOPLASMIC PH.54
3.2.12. NO MEASUREMENTS.56
3.3. ANOXIC METABOLISM OF 35S-NR TOBACCO PLANTS.58
3.3.1. NITRATE REDUCTASE ACTIVITY.58
3.3.2. ETHANOL PRODUCTION.59
3.3.3. LACTATE PRODUCTION.59
4. DISCUSSION.61
CONTENT
III
4.1. NORMOXIC METABOLISM AND ROOT ARCHITECTURE OF WT AND LNR*
H PLANTS.61
4.1.1. NITRATE REDUCTION AND ROOT MORPHOLOGY AND ARCHITECTURE.61
4.1.2. NITRATE REDUCTION AND ENERGY STATUS.63
4.2. ANOXIC METABOLISM OF WT AND LNR-H PLANTS.63
5. SUMMARY.
YY
.68
6. ZUSAMMENFASSUNG.71
7. ABBREVIATIONS.74
8. REFERENCES.76 |
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| illustrated | Illustrated |
| indexdate | 2024-11-22T17:34:56Z |
| institution | BVB |
| language | German |
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| physical | III, 84 S. Ill., graph. Darst. |
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| spelling | Stoimenova, Maria 1972- Verfasser (DE-588)124364616 aut Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase von Maria Stoimenova 2002 III, 84 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2002 Nitratreduktase (DE-588)4324240-6 gnd rswk-swf Anoxie (DE-588)4142575-3 gnd rswk-swf Wurzel (DE-588)4136391-7 gnd rswk-swf Tabak (DE-588)4136670-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tabak (DE-588)4136670-0 s Wurzel (DE-588)4136391-7 s Nitratreduktase (DE-588)4324240-6 s Anoxie (DE-588)4142575-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010065735&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stoimenova, Maria 1972- Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase Nitratreduktase (DE-588)4324240-6 gnd Anoxie (DE-588)4142575-3 gnd Wurzel (DE-588)4136391-7 gnd Tabak (DE-588)4136670-0 gnd |
| subject_GND | (DE-588)4324240-6 (DE-588)4142575-3 (DE-588)4136391-7 (DE-588)4136670-0 (DE-588)4113937-9 |
| title | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase |
| title_auth | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase |
| title_exact_search | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase |
| title_full | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase von Maria Stoimenova |
| title_fullStr | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase von Maria Stoimenova |
| title_full_unstemmed | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase von Maria Stoimenova |
| title_short | Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase |
| title_sort | normoxic and anoxic metabolism of nicotiana tabacum transformants lacking root nitrate reductase |
| topic | Nitratreduktase (DE-588)4324240-6 gnd Anoxie (DE-588)4142575-3 gnd Wurzel (DE-588)4136391-7 gnd Tabak (DE-588)4136670-0 gnd |
| topic_facet | Nitratreduktase Anoxie Wurzel Tabak Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010065735&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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