Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2002
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | München, Techn. Univ., Diss., 2002 |
| Physical Description: | 107, VII Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 |c Markus Zimmer |
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TABLE OF CONTENT
TABLE OF CONTENT
TABLE OF CONTENT I
LIST OF FIGURES V
LIST OF TABLES V
ABBREVIATIONS VI
SUMMARY 1
ZUSAMMENFASSUNG 3
1 INTRODUCTION 5
1.1
CLOSTRIDIUM PERFRINGENS 5
1.1.1 GENETIC ORGANIZATION OF VIRULENCE FACTORS FROM C.
PERFRINGENS
6
1.1.2 HISTOTOXIC DISEASES IN HUMANS CAUSED BY C.
PERFRINGENS 7
1.1.3 FOOD POISONING 8
1.1.4 DISEASES CAUSED BY C.
PERFRINGENS
IN POULTRY 10
1.2 BACTERIOPHAGES 11
1.2.1 GENERAL INTRODUCTION 11
1.2.2 LIFECYCLES 12
1.2.3 BACTERIOPHAGES OF C.
PERFRINGENS
13
1.3 LYSIS BY BACTERIOPHAGES 14
1.3.1 HOLINS
14
1.3.2 ENDOLYSINS 15
1.4 AIMS OF THIS WORK 16
2 MATERIALS AND METHODS 17
2.1 ORGANISMS, BACTERIOPHAGES AND PLASMIDS 17
2.2 MEDIA AND GROWTH CONDITIONS 18
2.3 ISOLATION OF BACTERIOPHAGES
19
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/965518884
TABLE OF CONTENT
2.4 PROPAGATION AND PURIFICATION OF PHAGES 20
2.5 DETERMINATION OF THE LYTIC RANGE OF THE ISOLATED BACTERIOPHAGES 21
2.6 ELECTRON MICROSCOPY 21
2.7 DNA MANIPULATION TECHNIQUES 22
2.7.1 DNA ISOLATION FROM BACTERIOPHAGES 22
2.7.2 DNA ISOLATION FROM C.
PERFRINGENS 22
2.7.3 DNA PRECIPITATION BY ETHANOL / ISOPROPANOL 22
2.7.4 LIGATION 23
2.7.5 ELECTROTRANSFORMATION 23
2.7.6 SEPARATION OF DNA FRAGMENTS BY AGAROSE GEL ELECTROPHORESIS (AGE)
24
2.7.7 PREPARATION OF DNA FRAGMENTS FROM AGAROSE GELS 26
2.7.8 POLYMERASE CHAIN REACTION (PCR) 26
2.7.9 PURIFICATION OF PCR PRODUCTS 26
2.8 ANALYSIS AND AMINO-TERMINAL SEQUENCING OF | 3626
STRUCTURAL PROTEINS 26
2.9 BIOINFORMATICS 29
2.10 CLONING, NUCLEOTIDE SEQUENCING, AND IDENTIFICATION OF THE COS-SITE
29
2.11 IDENTIFICATION OF
ATTPP' AND ATTBB' 30
2.12 CLONING AND FUNCTIONAL ANALYSIS OF THE HOLIN GENE
HOL3626 31
2.13 CLONING OF ENDOIYSIN §EN
EPLY3626 31
2.14 OVEREXPRESSION AND PURIFICATION OF PLY3626 32
2.15 LYTIC ACTIVITY AND SUBSTRATE SPECIFICITY 33
2.16 NUCLEOTIDE SEQUENCE ACCESSION NUMBERS 34
3 RESULTS 35
3.1 ISOLATION OF C.
PERFRINGENS PHAGES 35
3.2 NUCLEOTIDE SEQUENCING AND DETERMINATION OF THE COS-SITES 35
3.3 IDENTIFICATION AND NUCLEOTIDE SEQUENCE OF THE ATTACHMENT
SITES
ATTPP' AND ATTBB' 38
II
TABLE OF CONTENT
3.4 IDENTIFICATION AND ORGANIZATION OF | 3626 ORFS 40
3.5 STRUCTURAL PROTEINS OF | 3626 41
3.6 BIOINFORMATIC ANALYSIS OF 03626 GENE PRODUCTS 42
3.6.1 ORF 1 AND 2 45
3.6.2 ORF 3-5 45
3.6.3 ORF 7 AND 9 45
3.6.4 ORF 12/12.1 46
3.6.5 ORF 13 46
3.6.6 ORF 15 46
3.6.7 ORF 19 AND 20 47
3.6.8 ORF 22 49
3.6.9 ORF 23 ~ 51
3.6.10 ORF 24-25 51
3.6.11 ORF 26 51
3.6.12 ORF27 51
3.6.13 ORF 32 52
3.6.14 ORF 34 52
3.6.15 ORF 35 52
3.6.16 ORF 39 52
3.6.17 ORF 42 52
3.6.18 ORF 46 53
3.6.19 ORF 50 ' 53
3.7 OTHER FEATURES OF THE 03626 GENOME 53
3.8 FUNCTIONAL CHARACTERIZATION OF THE 03626 HOLIN 54
J
3.9 FUNCTIONAL CHARACTERIZATION OF PLY3626 55
3.9.1 RECOMBINANT PLY3626 CAN ONLY BE SYNTHESIZED IN
E. COLI
SUPPLEMENTED
WITH RARE TRNAS 55
3.9.2 THE ENDOLYSIN LYSES
C.
PERFRINGENS CELL WALLS 56
4 DISCUSSION 59
4.1 GENOME ORGANIZATION OF 03626 AND RELATIONSHIPS TO OTHER PHAGES 59
4.2 COHESIVE ENDS OF THE 03626 GENOME 60
TABLE OF CONTENT
4.3 INTEGRATION SITE 61
4.4 GENE PRODUCTS OF ({ 3626 62
4.5 A LINK BETWEEN SPORULATION AND LYSOGENY ? 63
4.6 LYSIS SYSTEM OF { 3626 65
REFERENCES 70
APPENDIX 88
ACKNOWLEDGMENTS VII
1
IV |
| any_adam_object | 1 |
| author | Zimmer, Markus 1971- |
| author_GND | (DE-588)124072453 |
| author_facet | Zimmer, Markus 1971- |
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| discipline | Biologie Lebensmitteltechnologie |
| format | Book |
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| indexdate | 2024-11-22T17:34:47Z |
| institution | BVB |
| language | English |
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| physical | 107, VII Bl. Ill., graph. Darst. |
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| spelling | Zimmer, Markus 1971- Verfasser (DE-588)124072453 aut Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 Markus Zimmer 2002 107, VII Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2002 Lyse Biologie (DE-588)4168396-1 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf Bakteriophagen (DE-588)4125508-2 gnd rswk-swf Enterotoxin (DE-588)4152342-8 gnd rswk-swf Clostridium perfringens (DE-588)4224052-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Clostridium perfringens (DE-588)4224052-9 s Bakteriophagen (DE-588)4125508-2 s Lyse Biologie (DE-588)4168396-1 s Genanalyse (DE-588)4200230-8 s Enterotoxin (DE-588)4152342-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010031940&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zimmer, Markus 1971- Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 Lyse Biologie (DE-588)4168396-1 gnd Genanalyse (DE-588)4200230-8 gnd Bakteriophagen (DE-588)4125508-2 gnd Enterotoxin (DE-588)4152342-8 gnd Clostridium perfringens (DE-588)4224052-9 gnd |
| subject_GND | (DE-588)4168396-1 (DE-588)4200230-8 (DE-588)4125508-2 (DE-588)4152342-8 (DE-588)4224052-9 (DE-588)4113937-9 |
| title | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 |
| title_auth | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 |
| title_exact_search | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 |
| title_full | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 Markus Zimmer |
| title_fullStr | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 Markus Zimmer |
| title_full_unstemmed | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 Markus Zimmer |
| title_short | Complete genome sequence and characterization of the lysis system of the temperate Clostridium perfringens bacteriophage phi 3626 |
| title_sort | complete genome sequence and characterization of the lysis system of the temperate clostridium perfringens bacteriophage phi 3626 |
| topic | Lyse Biologie (DE-588)4168396-1 gnd Genanalyse (DE-588)4200230-8 gnd Bakteriophagen (DE-588)4125508-2 gnd Enterotoxin (DE-588)4152342-8 gnd Clostridium perfringens (DE-588)4224052-9 gnd |
| topic_facet | Lyse Biologie Genanalyse Bakteriophagen Enterotoxin Clostridium perfringens Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010031940&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zimmermarkus completegenomesequenceandcharacterizationofthelysissystemofthetemperateclostridiumperfringensbacteriophagephi3626 |