The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity:
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| Format: | Book |
| Language: | English |
| Published: |
2002
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2002 |
| Physical Description: | 128 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity |c von Gordon Chan |
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TABLE OF CONTENTS
TABLE OF CONTENTS.4
ACKNOWLEDGEMENTS.6
SUMMARY.7
ZUSAMMENFASSUNG.9
ABBREVIATIONS.11
1. INTRODUCTION.13.
1.1 NKT CELLS.13
1.2 NK CELLS.17.
1.2.1 INHIBITORY RECEPTORS OF NK CELL ACTIVATION.17.
LY49 PROTEIN FAMILY.17.
CD94/NKG2.21.
1.2.2 STIMULATORY RECEPTORS OF NK CELL ACTIVATION.21.
NKP30, NKP44 AND NKP46.22.
LY49D AND LY49H.23.
CD94/NKG2C AND NKG2E.24.
NKG2D.24.
NKR-P1.25.
2B4.26.
CD16.26.
1.2.3 MECHANISMS OF NK CELL MEDIATED TUMOR LYSIS.27.
1.3 VAV FAMILY OF RHO GEFS.28.
1.3.1 VAV-1.32.
1.3.2 VAV-2. 35.
1.3.3 VAV-3.36.
1.4 VAV-1 , VAV-2 AND NK CELLS.37.
1.5 LSC/P115 RHO GEF.39.
1.6 AIMS OF THE PROJECT.43.
2. MATERIALS AND METHODS. 45.
2.1 MICE.
45.
2.2 CELL LINES.
45.
2.3 ANTIBODIES.45.
2.4 TISSUE HARVESTING AND CELL CULTURE.46.
2.4.1 SPLENECTOMY.46.
2.4.2 SPLENIC NK CELLS ENRICHMENT.46.
2.4.3 THYMECTOMY AND THYMOCYTE PREPARATION.49.
2.4.4 HARVESTING LIVER LYMPHOCYTES.50
2.5.5 HARVESTING LYMPHOCYTES FROM THE BONE MARROW.51
2.5 FLOW CYTOMETRY.
4
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/967167922
2.6 IN VIVO INJECTION OF ANTI-CD3.53
2.7 ELISA.53
2.8 CYTOTOXIC ASSAYS.55.
2.8.1 SPONTANEOUS CYTOTOXICITY.55.
2.8.2 ADCC.56.
2.9 CELL STIMULATION.57.
2.9.1 FCR MEDIATED MAPK ACTIVATION.57.
2.9.2 STIMULATION OF NK CELLS WITH TUMOR TARGETS.57.
2.10 WESTERN BLOT ANALYSIS.58.
3. RESULTS.60
3.1 DEVELOPMENT OF NK AND NK T CELLS IN VAV-1 -DEFICIENT MICE.60
3.2 VAV-1 IS NOT REQUIRED FOR ADCC AND CD16-MEDIATED MAPK ACTIVATION.65
3.3 SPONTANEOUS CYTOTOXICITY IS COMPROMISED IN VAV-1'- NK CELLS.70
3.4 DEVELOPMENT OF NK AND NK T CELLS IN VAV-2- AND VAV-1 /VAV-2
DEFICIENT MICE.78
3.5 VAV-1 AND VAV-2 ARE NOT REQUIRED FOR ADCC AND CD16-MEDIATED MAPK
ACTIVATION.75
3.6 SPONTANEOUS CYTOTOXICITY IN VAV-2 AND VAV-1/VAV-2 DEFICIENT NK
CELLS.81
3.7 ANALYSIS OF NK CELL FUNCTION IN LSC '" MICE.-.84
4. DISCUSSION.
4.1 VAV-1 REGULATES THE DEVELOPMENT OF NKT CELLS AND THE ACTIVATION OF
NK CELLS.92
4.2 LSC IS A NEGATIVE REGULATOR OF NK CELL CYTOTOXICITY.102
REFERENCES. 105
CURRICULUM VITAE.
.
PUBLICATIONS AND ABSTRACTS. 127
5 |
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| indexdate | 2024-11-22T17:34:43Z |
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| physical | 128 Bl. Ill., graph. Darst. |
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| spelling | Chan, Gordon Verfasser aut The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity von Gordon Chan 2002 128 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2002 Maus (DE-588)4169148-9 gnd rswk-swf Cytotoxizität (DE-588)4193696-6 gnd rswk-swf Natürliche Killerzelle (DE-588)4171290-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s Natürliche Killerzelle (DE-588)4171290-0 s Cytotoxizität (DE-588)4193696-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010014192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chan, Gordon The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity Maus (DE-588)4169148-9 gnd Cytotoxizität (DE-588)4193696-6 gnd Natürliche Killerzelle (DE-588)4171290-0 gnd |
| subject_GND | (DE-588)4169148-9 (DE-588)4193696-6 (DE-588)4171290-0 (DE-588)4113937-9 |
| title | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity |
| title_auth | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity |
| title_exact_search | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity |
| title_full | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity von Gordon Chan |
| title_fullStr | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity von Gordon Chan |
| title_full_unstemmed | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity von Gordon Chan |
| title_short | The role of Vav-1, Vav-2 and Lsc in NK T cell development and NK cell cytotoxicity |
| title_sort | the role of vav 1 vav 2 and lsc in nk t cell development and nk cell cytotoxicity |
| topic | Maus (DE-588)4169148-9 gnd Cytotoxizität (DE-588)4193696-6 gnd Natürliche Killerzelle (DE-588)4171290-0 gnd |
| topic_facet | Maus Cytotoxizität Natürliche Killerzelle Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010014192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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