Bioassay techniques for drug development:
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| Main Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam
Harwood
2001
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XII, 223 S. graph. Darst. |
| ISBN: | 9058230511 |
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| 245 | 1 | 0 | |a Bioassay techniques for drug development |c Atta-ur-Rahman and M. Iqbal Choudhary and William J. Thomsen |
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| 700 | 1 | |a Choudhary, Muhammad Iqbal |e Verfasser |4 aut | |
| 700 | 1 | |a Thomsen, William J. |e Verfasser |4 aut | |
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Record in the Search Index
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| adam_text | BIOASSAY TECHNIQUES FOR DRUG DEVELOPMENT ATTA-UR-RAHTNAN AND M. IQBAL
CHOUDHARY H.E.J. RESEARCH INSTITUTE OF CHEMISTRY UNIVERSITY OF KARACHI,
PAKISTAN AND WILLIAM J. THOMSEN ARENA PHARMACEUTICALS SAN DIEGO, USA U A
HARWOOD ACADEMIC PUBLISHERS ^ | | ^ AUSTRALIA * CANADA * FRANCE *
GERMANY * INDIA * JAPAN * LUXEMBOURG MALAYSIA * THE NETHERLANDS * RUSSIA
* SINGAPORE * SWITZERLAND CONTENTS PREFACE XI 1.0 GENERAL INTRODUCTION 1
PART A: BENCH-TOP AND PRIMARY BIOASSAY SCREENING 7 1.1 TOXICITY ASSAYS 9
1.1.1 BRINE-SHRIMP LETHALITY ASSAY 9 1.1.2 BRINE-SHRIMP MICROWELL
CYTOTOXICITY ASSAY 10 1.1.3 CROWN GALL TUMOR INHIBITION ASSAY (POTATO
DISC ANTITUMOR ASSAY) 12 1.1.4 ANIMAL TOXICITY ASSAY 14 1.2
ANTIMICROBIAL ASSAYS 14 1.2.1 ANTIBACTERIAL ASSAYS 16 1.2.1.1 AGAR
DIFFUSION ASSAY 16 1.2.1.2 AGAR DILUTION ASSAY 17 1.2.1.2.1 MICROTITRE
PLATE METHOD 18 1.2.1.3 DIRECT BIOAUTOBIOGRAPHY METHOD 19 1.2.2
ANTIFUNGAL ASSAYS 22 1.2.2.1 AGAR TUBE DILUTION ASSAY 22 1.2.2.2 DIRECT
BIOAUTOBIOGRAPHY METHOD 24 1.3 ANTIVIRAL AND ANTICANCER ASSAYS 25 1.3.1
ANTI-HIV ASSAY 25 1.3.2 ANTICANCER ASSAYS 27 1.3.2.1 IN VITRO ANTICANCER
SCREENING 28 1.3.3 HCT CYTOTOXICITY ASSAY 37 1.4 ANTIMITOTIC ASSAY 39
1.4.1 ANTIMITOTIC ASSAY USING SEA URCHIN EGGS 39 1.5 GENOTOXICITY ASSAYS
41 1.5.1 SOS CHROMTEST FOR GENOTOXICITY 41 1.5.2 AMES TEST FOR DETECTING
CARCINOGENS AND MUTAGENS 44 1.5.3 ANTIPHAGE ACTIVITY ASSAY 47 1.6 ASSAYS
FOR CONTROL OF TROPICAL DISEASES 48 1.6.1 ANTIMALARIAL ASSAYS 48 1.6.1.1
96 WELL PLATE IN VITRO SCHIZONTICIDAL ASSAY 49 1.6.1.2 SUPPRESSIVE TEST
OF BLOOD SCHIZONTICIDAL ACTION (PETER S TEST) 50 1.6.1.3 RANE TEST OF
BLOOD SCHIZONTICIDAL ACTIVITY 51 1.6.2 LARVICIDAL ASSAY 51 VI CONTENTS
1.6.3 MOLLUSCICIDAL ASSAY 52 1.6.4 PISCICIDAL ASSAY 53 1.6.5 AMOEBICIDAL
ASSAY 54 1.6.6 CERCARICIDAL ASSAY 59 1.6.7 LEISHMANICIDIC ACTIVITY 60
1.6.7.1 IN VITRO LEISHMANICIDAL ASSAY (PROMASTIGOTES) 60 1.6.7.2 IN
VITRO LEISHMANICIDAL ASSAY (AMASTIGOTES) 62 1.6.7.3 LEISHMANICIDAL
ACTIVITY IN VIVO 64 1.7 ASSAYS FOR AGROCHEMICALS 65 1.7.1 LEMNA MINOR
FOR PHYTOTOXICITY AND GROWTH-STIMULATING ASSAY 65 1.7.2 CONTACT TOXICITY
INSECTICIDAL ASSAY 67 1.7.3 INSECT ANTIFEEDANT ASSAYS 68 1.7.3.1
LEAF-CHOICE ASSAY 68 1.7.3.2 ARTIFICIAL DIET FEEDING BIOASSAY 69 1.7.4
NEMATICIDAL ASSAY 72 1.8 HEPATOTOXICITY ASSAYS 74 1.8.1 ANTIHEPATOTOXIC
ACTIVITY USING CARBON TETRACHLORIDE-INDUCED CYTOTOXICITY 74 1.8.2 IN
VIVO HEPATOPROTECTIVE ASSAY 76 1.8.3 ANTIHEPATOTOXIC ACTIVITY USING
GALACTOSAMINE INDUCED CYTOTOXICITY 78 1.9 HYPOGLYCEMIC/ANTIDIABETIC
ACTIVITY ASSAYS 82 1.9.1 ANTIDIABETIC ACTIVITY ASSAY ON NORMAL AND
ALLOXAN-DIABETIC RABBITS 82 1.9.2 ANTIDIABETIC ACTIVITY ASSAY (GOD-PAP
METHOD) 84 1.9.3 HYPOGLYCEMIC ACTIVITY ASSAY 85 1.10 DIURETIC ACTIVITY
ASSAY 87 1.11 ANTHEHNINTIC ACTIVITY ASSAYS 88 1.11.1 IN VITRO
ANTHEHNINTIC ASSAY 88 1.11.2 IN VIVO ANTHELMINTIC ASSAY 90 1.12
ANTIFERTILITY/ANTI-IMPLANTATION ASSAYS 90 1.12.1 ANTIFERTILITY ACTIVITY
90 1.12.2 ANTI-IMPLANTATION ACTIVITY 91 1.13 IN VITRO ASSAY FOR PLATELET
AGGREGATION 92 1.14 ANTI-INFLAMMATORY ASSAY 94 1.14.1 RAT PAW EDEMA
ASSAY 94 1.15 IMMUNOMODULATING ASSAY 95 1.15.1 HEMOLYTIC PLAQUE ASSAY
95 1.16 ANTIEPILEPTIC (ANTICORIVULSANT) ASSAY 96 1.16.1 PILOCARPINE
MODEL 97 1.17 ANALGESIC ASSAYS 100 1.17.1 WRITHING SYNDROME METHOD 100
CONTENTS VII 1.17.2 HOT PLATE METHOD 100 1.18 GASTROPROTECTIVE/ANTIULCER
ASSAYS 101 1.18.1 GASTROPROTECTIVE ASSAY 101 1.18.2 ANTI-ULCEROGENIC
ASSAY-1 102 1.18.3 ANTI-ULCEROGENIC ASSAY-2 103 1.19 RADIOLABELLING
BIOASSAYS 103 1.19.1 MITOGENIC ACTIVITY ASSAY 103 1.20 ANTI-EMETIC ASSAY
106 PART B: HIGH-THROUGHPUT SCREENING 107 2.1 INTRODUCTION 109 2.1.1
CHARACTERISTICS OF AN IDEAL SCREENING ASSAY FOR SCREENING NATURAL
PRODUCT EXTRACTS 109 2.1.1.1 BIOCHEMICAL OR CELL BASED ASSAYS 110
2.1.1.2 ASSAY FORMAT 110 2.1.1.3 REAGENT SOURCE 111 2.1.1.4 ASSAY
SIGNAL-TO-NOISE RATIO 112 2.1.1.5 ASSAY INCUBATION 112 2.1.1.6 ASSAY
MANIPULATIONS 113 2.1.1.7 DMSO COMPATIBILITY 113 2.1.1.8 SCREENING
SOURCE MATERIAL COMPATIBILITY 113 2.2 ENZYME ASSAYS 114 2.2.1
INTRODUCTION 114 2.2.2 CONSIDERATIONS FOR DEVELOPMENT OF ENZYME ASSAYS
114 2.2.2.1 SELECTION OF ENZYME SOURCE 115 2.2.2.2 SELECTION OF ENZYME
CONCENTRATION 116 2.2.2.3 SELECTION OF SUBSTRATE AND SUBSTRATE
CONCENTRATION 116 2.2.2.4 SELECTION OF COFACTOR CONCENTRATIONS 121
2.2.2.5 SELECTION OF ASSAY ENDPOINT 122 2.2.2.6 SELECTION OF ASSAY PH
122 2.2.2.7 SELECTION OF ASSAY TEMPERATURE 123 2.2.2.8 DMSO
COMPATIBILITY 123 2.2.2.9 EXTRACT COMPATIBILITY 123 2.2.2.10 INHIBITOR
PROFILES 123 2.2.2.11 COMPETITIVE INHIBITION 124 2.2.2.12 UNCOMPETITIVE
INHIBITION 125 2.2.2.13 NONCOMPETITIVE INHIBITION 125 2.2.3 DEVELOPMENT
OF A HIGH THROUGHPUT ENZYME ASSAY FOR NATURAL PRODUCT SCREENING: AN
EXAMPLE 126 2.2.4 SOME COMMON IN VITRO ENZYME-BASED BIOASSAYS 133 2.2 A
A PROTEASE INHIBITION ASSAYS 133 2.2.4.2 TYROSINASE INHIBITION ASSAY 137
VIII CONTENTS 2.2.4.3 HYALURONIDASE INHIBITION ASSAY 139 2.2.4.4
ACETYLCHOLINESTERASE INHIBITION ASSAY 142 2.2 A A.I SPECTROPHOTOMETRIC
ASSAY 143 2.2.4.5 P-LACTAMASE INHIBITION ASSAY 145 2.2.4.5.1
SPECTROPHOTOMETRIC ASSAY 146 2.2.4.6 5-LIPOXYGENASE INHIBITION ASSAY 148
2.2.4.6.1 SPECTROPHOTOMETRIC ASSAY 149 2.3 CELL-BASED RECEPTOR
FUNCTIONAL ASSAYS 152 2.3.1 INTRODUCTION 152 2.3.1.1 UTILITY OF RECEPTOR
FUNCTIONAL ASSAYS 152 2.3.2 FUNCTIONAL ASSAYS FOR G-PROTEIN COUPLED
RECEPTORS 152 2.3.2.1 GS AND GI COUPLED RECEPTOR SIGNAL TRANSDUCTION
PATHWAYS 152 2.3.2.2 HIGH THROUGHPUT FUNCTIONAL ASSAYS FOR GS AND GI
COUPLED RECEPTORS 155 2.3.3 COMMON CELL-BASED RECEPTOR FUNCTIONAL ASSAYS
155 2.3.3.1 SPA CAMP ASSAY 155 2.3.3.2 FLASHPLATE CAMP ASSAY 156 2.3.3.3
ELISA-BASED CAMP ASSAYS 157 2.3.3.4 CAMP RESPONSE ELEMENT REPORTER GENE
ASSAY 160 2.3.3.5 GQ-COUPLED RECEPTOR SIGNAL TRANSDUCTION PATHWAYS 161
2.3.3.5.1 INTRODUCTION 161 2.3.3.5.2 HIGH THROUGHPUT ASSAYS FOR
GQ-COUPLED RECEPTORS 164 2.3.3.6 HIGH THROUGHPUT FUNCTIONAL ASSAYS FOR
LIGAND-GATED ION CHANNELS 166 2.3.3.7 RECEPTOR PROTEIN TYROSINE KINASE
FUNCTIONAL ASSAYS 167 2.3.3.7.1 INTRODUCTION 167 2.3.3.7.2
RPTK-ASSOCIATED SIGNAL TRANSDUCTION PATHWAYS 167 2.3.3.7.3 HIGH
THROUGHPUT FUNCTIONAL ASSAYS .FOR RPTK S 169 2.3.3.7.4 INTRACELLULAR
CALCIUM ASSAY 169 2.3.3.7.5 PROLIFERATION ASSAYS 170 2.3.3.8 CYTOKINE
RECEPTOR FUNCTIONAL ASSAYS 173 2.3.3.8.1 CYTOKINE RECEPTOR SIGNAL
TRANSDUCTION PATHWAYS 173 2.3.3.8.2 FUNCTIONAL ASSAYS FOR CYTOKINE
RECEPTORS 173 2.3.3.9 MIXED RECEPTOR FUNCTIONAL ASSAY 174 CONTENTS IX
2.3.3.9.1 MELANOPHORE FUNCTIONAL RECEPTOR ASSAY TECHNOLOGY 174 2.3.3.10
RECEPTOR SELECTION AND AMPLIFICATION FUNCTIONAL RECEPTOR ASSAY
TECHNOLOGY 178 2.4 RADIOLIGAND BINDING ASSAYS 179 2.4.1 INTRODUCTION 179
2.4.2 IMPORTANT CONSIDERATIONS FOR DEVELOPING RADIOLIGAND BINDING ASSAYS
180 2.4.2.1 RECEPTOR SOURCE AND ASSAY CONCENTRATION 180 2.4.2.1.1
NONRECOMBINANT HUMAN CELL LINES 180 2.4.2.1.2 MEMBRANE PREPARATIONS FROM
NONRECOMBINANT CELLS AND TISSUES 181 2.4.2.1.3 RECOMBINANT RECEPTOR
CONTAINING CELLS OR MEMBRANES 181 2.4.2.1.4 SELECTION OF RADIOLIGAND AND
ASSAY CONCENTRATION 182 2.4.2.1.5 SELECTION OF ASSAY INCUBATION
CONDITIONS 184 2.4.2.1.6 SELECTION OF ASSAY BUFFER 184 2.4.2.1.7
SELECTION OF ASSAY VOLUME 184 2.4.2.1.8 SELECTION OF A METHODOLOGY FOR
SEPARATION OF FREE AND BOUND RADIOLIGAND 185 2.4.3 STEPS IN DEVELOPING
AND VALIDATING RADIOLIGAND BINDING ASSAYS 185 2.4.3.1 CELL MEMBRANE
LINEARITY EVALUATION 186 2.4.3.2 ASSOCIATION AND DISSOCIATION TIME
COURSES 187 2.4.3.3 SATURATION ISOTHERM EVALUATION 192 2.4.3.4
COMPETITION STUDIES 194 2.4.4 ALTERNATIVE RADIOLIGAND BINDING ASSAYS 198
2.4.4.1 FLASHPLATE ASSAYS 198 2.4.4.2 SCINTILLATION PROXIMITY ASSAYS 200
2.4.4.3 SCINTISTRIP PLATE ASSAYS 201 2.4.4.4 FLUORESCENCE BASED RECEPTOR
BINDING 203 2.4.4.4.1 TIME RESOLVED FLUORESCENCE 203 2.4.4.4.2
FLUORESCENCE POLARIZATION 203 2.5 REFERENCES 205 2.6 INDEX 219
|
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| isbn | 9058230511 |
| language | English |
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| spelling | Atta-ur-Rahman 1942- Verfasser (DE-588)111146356 aut Bioassay techniques for drug development Atta-ur-Rahman and M. Iqbal Choudhary and William J. Thomsen Amsterdam Harwood 2001 XII, 223 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Arzneimittelentwicklung Bioassay Biological Assay Drug Design Choudhary, Muhammad Iqbal Verfasser aut Thomsen, William J. Verfasser aut GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009993503&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Atta-ur-Rahman 1942- Choudhary, Muhammad Iqbal Thomsen, William J. Bioassay techniques for drug development Arzneimittelentwicklung Bioassay Biological Assay Drug Design |
| title | Bioassay techniques for drug development |
| title_auth | Bioassay techniques for drug development |
| title_exact_search | Bioassay techniques for drug development |
| title_full | Bioassay techniques for drug development Atta-ur-Rahman and M. Iqbal Choudhary and William J. Thomsen |
| title_fullStr | Bioassay techniques for drug development Atta-ur-Rahman and M. Iqbal Choudhary and William J. Thomsen |
| title_full_unstemmed | Bioassay techniques for drug development Atta-ur-Rahman and M. Iqbal Choudhary and William J. Thomsen |
| title_short | Bioassay techniques for drug development |
| title_sort | bioassay techniques for drug development |
| topic | Arzneimittelentwicklung Bioassay Biological Assay Drug Design |
| topic_facet | Arzneimittelentwicklung Bioassay Biological Assay Drug Design |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009993503&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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