Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2002 |
| Beschreibung: | IV, 126 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text |
TABLE OF CONTENTS
1
INTRODUCTION._._._._._.1
1.1 MOTIVATION AND BACKGROUND.1
1.2 TOPIC OF THIS WORK.3
1.2.1 CHARGE TRANSFER IN MODIFIED DNA.3
1.2.2 COMPLEX BETWEEN FLUORESCEIN AND AN ENGINEERED LIPOCALIN PROTEIN.5
1.3 OVERVIEW OF THE CONTENT.6
2 EXPERIMENTAL
METHODS._.7
2.1 TIME-RESOLVED ABSORPTION MEASUREMENTS.7
2.1.1 THE PUMP-PROBE TECHNIQUE.7
2.1.2 DIFFERENTIAL ABSORPTION SPECTROSCOPY.8
2.1.3 THE TITANIUM:SAPPHIRE FEMTOSECOND LASER SYSTEM.10
2.1.4 CALCULATION OF THE TRANSIENT ABSORPTION SIGNAL.13
2.1.5 NUMERICAL ANALYSIS OF THE MEASUREMENTS.14
2.1.6 FEMTOSECOND PUMP-PROBE MEASUREMENTS WITH EXCITATION AT 390 NM.15
2.1.7 NANOSECOND PUMP-PROBE MEASUREMENTS.15
2.1.8 BROADBAND FEMTOSECOND ABSORPTION SPECTROSCOPY.15
2.2 STEADY-STATE MEASUREMENTS.16
2.3 SAMPLE PREPARATION.16
2.3.1 THE DNA OLIGONUCLEOTIDES.16
2.3.2 THE FLUA FLUORESCEIN COMPLEX.17
3 THEORETICAL BASICS--._.19
3.1 PHOTOINDUCED CHARGE TRANSFER.19
3.2 FUNDAMENTALS OF NONADIABATIC ELECTRON TRANSFER THEORY.20
3.2.1 CLASSICAL THEORY.21
3.2.2 QUANTUM MECHANICAL THEORY.24
3.2.3 SUPEREXCHANGE
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/965168069
II
TABLE OF CONTENTS
4.2.1 STEADY-STATE SPECTROSCOPY.31
4.2.2 TRANSIENT ABSORPTION SPECTROSCOPY.35
4.3 DISCUSSION.41
4.3.1 HOLE TRANSFER IN 3'-X
+G AND 3'-X+Z.44
4.3.2 HOLE TRANSFER IN 3'-X
+AG AND S'-X^AAG.45
4.3.3 HOLE TRANSFER IN 3'-X
+AZ AND 3'-X+AAZ.46
4.3.4 COMPARISON TO PREVIOUS RESULTS.46
4.3.5 DISTANCE DEPENDENCE OF THE ACTIVATION ENERGY.47
5 EXCITED-STATE PHOTOPHYSICS OF ACMA SELECTIVELY INTERCALATED IN DUPLEX
DNA. 51
5.1 INTRODUCTION.51
5.2 PHOTOPHYSICS OF ACMA IN DNA.52
5.3 COMPARISON TO THE SOLUTION PHASE.54
5.4 CONCLUSIONS.58
6 DISTANCE-DEPENDENT ACTIVATION ENERGIES FOR HOLE INJECTION INTO DUPLEX
DNA. 61
6.1 PREPARATIONS FOR THE MEASUREMENTS.61
6.1.1 ACCESSIBLE TEMPERATURE RANGE.61
6.1.2 CHARACTERIZATION OF THE SAMPLES IN BUFFER/SUCROSE SOLUTION.62
6.2 TEMPERATURE-DEPENDENT MEASUREMENTS.63
6.3 DISCUSSION.69
6.3.1 ANALYSIS OF THE ACTIVATION ENERGIES AND RATE CONSTANTS.69
6.3.2 DISTANCE DEPENDENCE OF THE REORGANIZATION ENERGY.70
6.3.3 SENSITIVITY OF THE REORGANIZATION ENERGY TO PARAMETERS.
)
.76
6.3.4 COMPARISON BETWEEN THEORY AND EXPERIMENT.76
6.4 CONCLUSIONS.81
7 NUCLEOBASE OXIDATION KINETICS AND THE ROLE OF THE NUCLEOBASE
ENVIRONMENT. 83
7.1 HOLE TRANSFER TO VARIOUS PURINE NUCLEOBASES.83
7.1.1 EXPERIMENTAL RESULTS.85
7.1.2 DISCUSSION.88
7.2 HOLE TRANSFER ACROSS A (T)YY BRIDGE.92
7.3 INTERSTRAND HOLE TRANSFER.93
7.4 COOPERATIVE EFFECTS OF ADJACENT BASES.95
8 ULTRAFAST ELECTRON TRANSFER IN THE COMPLEX BETWEEN FLUORESCEIN AND AN
ENGINEERED LIPOCALIN PROTEIN.99
8.1 RESULTS.99
8.1.1 STEADY-STATE SPECTROSCOPY OF FREE AND PROTEIN-BOUND FLUORESCEIN.99
TABLE OF CONTENTS
III
8.1.2 FEMTOSECOND TRANSIENT ABSORPTION SPECTROSCOPY.103
8.2 DISCUSSION.108
8.2.1 MECHANISM OF FLUORESCENCE QUENCHING IN FLUA-FL.108
8.2.2 CHARACTERIZATION OF THE FLUORESCING MINORITY.110
8.3 CONCLUSIONS.ILL
9
SUMMARY._.
113
REFERENCES_.117 |
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| classification_tum | CHE 164d CHE 860d CHE 189d |
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| discipline | Physik Chemie |
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| institution | BVB |
| language | English |
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| physical | IV, 126 S. Ill., graph. Darst. |
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| spelling | Hess, Stephan Verfasser aut Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein Stephan Hess 2002 IV, 126 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2002 Femtosekundenspektroskopie (DE-588)4276916-4 gnd rswk-swf Fotochemische Reaktion (DE-588)4138378-3 gnd rswk-swf Elektronentransfer (DE-588)4151907-3 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Acridin (DE-588)4347531-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content DNS (DE-588)4070512-2 s Elektronentransfer (DE-588)4151907-3 s Acridin (DE-588)4347531-0 s Fotochemische Reaktion (DE-588)4138378-3 s Femtosekundenspektroskopie (DE-588)4276916-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009962749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hess, Stephan Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein Femtosekundenspektroskopie (DE-588)4276916-4 gnd Fotochemische Reaktion (DE-588)4138378-3 gnd Elektronentransfer (DE-588)4151907-3 gnd DNS (DE-588)4070512-2 gnd Acridin (DE-588)4347531-0 gnd |
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| title | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein |
| title_auth | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein |
| title_exact_search | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein |
| title_full | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein Stephan Hess |
| title_fullStr | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein Stephan Hess |
| title_full_unstemmed | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein Stephan Hess |
| title_short | Dynamics of photoinduced charge transfer processes in modified DNA and an engineered protein |
| title_sort | dynamics of photoinduced charge transfer processes in modified dna and an engineered protein |
| topic | Femtosekundenspektroskopie (DE-588)4276916-4 gnd Fotochemische Reaktion (DE-588)4138378-3 gnd Elektronentransfer (DE-588)4151907-3 gnd DNS (DE-588)4070512-2 gnd Acridin (DE-588)4347531-0 gnd |
| topic_facet | Femtosekundenspektroskopie Fotochemische Reaktion Elektronentransfer DNS Acridin Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009962749&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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